Salmonella phage 7-11
Average proteome isoelectric point is 6.61
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 151 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|G0X509|G0X509_9CAUD Uncharacterized protein OS=Salmonella phage 7-11 OX=1054968 PE=4 SV=1
MM1 pKa = 7.63 KK2 pKa = 10.5 VGLLIANGGDD12 pKa = 3.51 GSASIHH18 pKa = 5.42 YY19 pKa = 9.81 FKK21 pKa = 11.19 DD22 pKa = 3.69 LIYY25 pKa = 10.78 ASQLQDD31 pKa = 3.77 CDD33 pKa = 3.87 THH35 pKa = 8.74 CEE37 pKa = 4.12 TFGLNDD43 pKa = 3.48 SMDD46 pKa = 4.14 IIDD49 pKa = 4.2 VPEE52 pKa = 4.26 GFYY55 pKa = 10.79 PPGGFSDD62 pKa = 4.38 YY63 pKa = 11.16 EE64 pKa = 3.95 WDD66 pKa = 4.16 LEE68 pKa = 4.34 EE69 pKa = 4.59
Molecular weight: 7.65 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.83
IPC2_protein 3.719
IPC_protein 3.656
Toseland 3.452
ProMoST 3.808
Dawson 3.668
Bjellqvist 3.91
Wikipedia 3.63
Rodwell 3.49
Grimsley 3.363
Solomon 3.63
Lehninger 3.592
Nozaki 3.795
DTASelect 4.012
Thurlkill 3.528
EMBOSS 3.643
Sillero 3.783
Patrickios 0.223
IPC_peptide 3.63
IPC2_peptide 3.745
IPC2.peptide.svr19 3.765
Protein with the highest isoelectric point:
>tr|G0X578|G0X578_9CAUD Uncharacterized protein OS=Salmonella phage 7-11 OX=1054968 PE=4 SV=1
MM1 pKa = 7.29 RR2 pKa = 11.84 QSAFQLLKK10 pKa = 11.08 ASLDD14 pKa = 3.95 NNNGAPVSQVQRR26 pKa = 11.84 EE27 pKa = 3.96 VLLNKK32 pKa = 9.95 KK33 pKa = 9.2 VLYY36 pKa = 10.14 RR37 pKa = 11.84 YY38 pKa = 9.04 ARR40 pKa = 11.84 PTQQVFGTITAFTGNIQRR58 pKa = 11.84 VWVKK62 pKa = 9.53 WSDD65 pKa = 3.52 GAVEE69 pKa = 3.92 ARR71 pKa = 11.84 SIKK74 pKa = 10.28 SLEE77 pKa = 4.17 VIKK80 pKa = 10.97
Molecular weight: 9.06 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.27
IPC2_protein 9.736
IPC_protein 10.189
Toseland 10.701
ProMoST 10.365
Dawson 10.789
Bjellqvist 10.438
Wikipedia 10.95
Rodwell 11.213
Grimsley 10.833
Solomon 10.877
Lehninger 10.847
Nozaki 10.672
DTASelect 10.438
Thurlkill 10.687
EMBOSS 11.096
Sillero 10.716
Patrickios 10.994
IPC_peptide 10.877
IPC2_peptide 9.004
IPC2.peptide.svr19 8.741
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
151
0
151
26783
27
976
177.4
20.0
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
7.919 ± 0.315
1.217 ± 0.102
6.239 ± 0.167
7.158 ± 0.309
4.144 ± 0.197
6.963 ± 0.264
2.061 ± 0.114
5.119 ± 0.156
6.624 ± 0.241
7.113 ± 0.202
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.953 ± 0.093
5.022 ± 0.149
3.696 ± 0.139
4.073 ± 0.226
5.395 ± 0.209
6.138 ± 0.18
5.686 ± 0.231
7.143 ± 0.194
1.729 ± 0.14
3.607 ± 0.15
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here