Pseudoflavonifractor capillosus ATCC 29799
Average proteome isoelectric point is 6.4
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 4804 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A6NRS0|A6NRS0_9FIRM Uncharacterized protein OS=Pseudoflavonifractor capillosus ATCC 29799 OX=411467 GN=BACCAP_00898 PE=4 SV=1
MM1 pKa = 7.48 KK2 pKa = 10.13 GKK4 pKa = 10.5 KK5 pKa = 9.17 IFSAALSLSLAAVLCLSGCTSAAAEE30 pKa = 4.38 DD31 pKa = 4.57 SNASSAVMPSVPPAEE46 pKa = 4.33 TTVDD50 pKa = 3.71 VPTSDD55 pKa = 3.79 PLAPPEE61 pKa = 4.39 GPVYY65 pKa = 10.47 TIAQQHH71 pKa = 5.24 QPDD74 pKa = 4.08 LVRR77 pKa = 11.84 VQGTVSVDD85 pKa = 2.88 GDD87 pKa = 3.92 RR88 pKa = 11.84 ILVTNSNEE96 pKa = 3.38 WAQYY100 pKa = 9.59 PEE102 pKa = 4.15 IVLSLEE108 pKa = 3.86 KK109 pKa = 10.28 DD110 pKa = 3.66 TLILNAVDD118 pKa = 4.63 GSAMAAEE125 pKa = 4.7 DD126 pKa = 4.01 LRR128 pKa = 11.84 EE129 pKa = 4.11 GEE131 pKa = 4.08 YY132 pKa = 10.84 VYY134 pKa = 10.83 AYY136 pKa = 9.89 VSPAMTDD143 pKa = 3.18 SLPPITTAAVILAAIPADD161 pKa = 3.79 YY162 pKa = 10.61 AVPAYY167 pKa = 10.71 SIVEE171 pKa = 3.95 QVNVWEE177 pKa = 4.74 GRR179 pKa = 11.84 TSILMSDD186 pKa = 3.74 DD187 pKa = 3.15 VTYY190 pKa = 11.55 SMSDD194 pKa = 3.17 AEE196 pKa = 4.62 LLAGPGFTCGTITLADD212 pKa = 3.56 ITPGCGLLAWYY223 pKa = 10.29 DD224 pKa = 3.61 MLAEE228 pKa = 4.44 SFPAQAWPVKK238 pKa = 10.34 VMAIPSPYY246 pKa = 9.93 DD247 pKa = 2.77 AWAEE251 pKa = 4.02 LEE253 pKa = 4.1 PGRR256 pKa = 11.84 LAVNGTEE263 pKa = 4.21 VEE265 pKa = 4.24 LTEE268 pKa = 5.07 AEE270 pKa = 4.07 QPYY273 pKa = 10.07 VEE275 pKa = 5.48 GEE277 pKa = 4.15 TLMVPVRR284 pKa = 11.84 AFAEE288 pKa = 4.02 ALGCEE293 pKa = 4.78 VIWNADD299 pKa = 3.4 QPEE302 pKa = 4.67 SISVWGEE309 pKa = 3.35 GAEE312 pKa = 4.05 YY313 pKa = 10.94 LLTLGSDD320 pKa = 3.64 RR321 pKa = 11.84 AATGDD326 pKa = 3.79 GGDD329 pKa = 3.7 GTALTGAVTARR340 pKa = 11.84 NGVSFLPADD349 pKa = 4.42 DD350 pKa = 5.37 LLNLHH355 pKa = 6.08 NVRR358 pKa = 11.84 AEE360 pKa = 4.15 GLWPVV365 pKa = 3.43
Molecular weight: 38.38 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.727
IPC2_protein 3.821
IPC_protein 3.808
Toseland 3.605
ProMoST 3.948
Dawson 3.783
Bjellqvist 3.935
Wikipedia 3.681
Rodwell 3.643
Grimsley 3.516
Solomon 3.77
Lehninger 3.732
Nozaki 3.884
DTASelect 4.075
Thurlkill 3.643
EMBOSS 3.694
Sillero 3.923
Patrickios 0.922
IPC_peptide 3.77
IPC2_peptide 3.91
IPC2.peptide.svr19 3.82
Protein with the highest isoelectric point:
>tr|A6NST1|A6NST1_9FIRM Uncharacterized protein OS=Pseudoflavonifractor capillosus ATCC 29799 OX=411467 GN=BACCAP_01260 PE=4 SV=1
MM1 pKa = 7.57 IFFGMINHH9 pKa = 6.64 TPSFSFLAQILWSSRR24 pKa = 11.84 QAARR28 pKa = 11.84 RR29 pKa = 11.84 ARR31 pKa = 11.84 FLIFWHH37 pKa = 6.5 PNIIAAQNRR46 pKa = 11.84 KK47 pKa = 9.01 AVNPP51 pKa = 4.06
Molecular weight: 5.97 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.393
IPC2_protein 10.833
IPC_protein 12.442
Toseland 12.603
ProMoST 13.1
Dawson 12.603
Bjellqvist 12.603
Wikipedia 13.086
Rodwell 12.193
Grimsley 12.647
Solomon 13.1
Lehninger 12.998
Nozaki 12.603
DTASelect 12.603
Thurlkill 12.603
EMBOSS 13.1
Sillero 12.603
Patrickios 11.974
IPC_peptide 13.1
IPC2_peptide 12.091
IPC2.peptide.svr19 9.127
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
4804
0
4804
1302578
17
4122
271.1
30.0
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
9.327 ± 0.045
1.649 ± 0.02
5.672 ± 0.03
6.816 ± 0.037
3.867 ± 0.027
7.82 ± 0.041
1.817 ± 0.016
5.403 ± 0.03
4.683 ± 0.04
9.885 ± 0.053
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.801 ± 0.021
3.342 ± 0.028
4.265 ± 0.026
3.523 ± 0.025
5.677 ± 0.047
5.794 ± 0.032
5.762 ± 0.049
7.147 ± 0.034
1.152 ± 0.013
3.598 ± 0.026
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here