Epstein-Barr virus (strain AG876) (HHV-4) (Human herpesvirus 4)

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Peploviricota; Herviviricetes; Herpesvirales; Herpesviridae; Gammaherpesvirinae; Lymphocryptovirus; Human gammaherpesvirus 4

Average proteome isoelectric point is 6.76

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 82 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>sp|Q1HVB5|BNL2B_EBVA8 Uncharacterized protein BNLF2b OS=Epstein-Barr virus (strain AG876) OX=82830 GN=BNLF2b PE=3 SV=1
MM1 pKa = 7.25EE2 pKa = 6.06HH3 pKa = 7.2DD4 pKa = 4.84LEE6 pKa = 5.12RR7 pKa = 11.84GPPGPRR13 pKa = 11.84RR14 pKa = 11.84PPRR17 pKa = 11.84GPPLSSSLGLALLLLLLALLFWLYY41 pKa = 10.78IVMSDD46 pKa = 3.13WTGGALLVLYY56 pKa = 10.28SFALILIIIILIIFIFRR73 pKa = 11.84RR74 pKa = 11.84DD75 pKa = 3.73LLCPLGALCLLLLMITLLLIALWNLHH101 pKa = 5.13GQALYY106 pKa = 10.82LGIVLFIFGCLLVLGLWIYY125 pKa = 10.87LLEE128 pKa = 4.3ILWRR132 pKa = 11.84LGATIWQLLAFFLAFFLDD150 pKa = 5.18LILLIIALYY159 pKa = 9.75LQQNWWTLLVDD170 pKa = 5.12LLWLLLFLAILIWMYY185 pKa = 10.53YY186 pKa = 8.99HH187 pKa = 6.85GQRR190 pKa = 11.84HH191 pKa = 5.39SDD193 pKa = 3.35EE194 pKa = 4.43HH195 pKa = 7.75HH196 pKa = 6.96HH197 pKa = 7.46DD198 pKa = 4.28DD199 pKa = 5.28SLPHH203 pKa = 6.25PQQATDD209 pKa = 3.84DD210 pKa = 4.24SGHH213 pKa = 6.22EE214 pKa = 4.08SDD216 pKa = 4.7SNSNEE221 pKa = 3.73GRR223 pKa = 11.84HH224 pKa = 5.67HH225 pKa = 6.75LLVTGAGDD233 pKa = 4.29GPPLCSQNLGAPGGGPDD250 pKa = 5.41NGPQDD255 pKa = 4.7PDD257 pKa = 3.47NTDD260 pKa = 3.56DD261 pKa = 5.97NGPQDD266 pKa = 5.0PDD268 pKa = 3.47NTDD271 pKa = 3.56DD272 pKa = 5.97NGPQDD277 pKa = 5.0PDD279 pKa = 3.47NTDD282 pKa = 3.56DD283 pKa = 5.97NGPQDD288 pKa = 4.43PDD290 pKa = 3.16NTADD294 pKa = 4.25NGPHH298 pKa = 7.25DD299 pKa = 5.18PLPHH303 pKa = 6.42NPSDD307 pKa = 3.85SAGNDD312 pKa = 3.31GGPPNLTEE320 pKa = 3.9EE321 pKa = 4.49VEE323 pKa = 4.4NKK325 pKa = 10.5GGDD328 pKa = 3.31RR329 pKa = 11.84GPPSMTDD336 pKa = 2.93GGGGDD341 pKa = 3.49PHH343 pKa = 8.36LPTLLLGTSGSGGDD357 pKa = 4.67DD358 pKa = 4.4DD359 pKa = 6.1DD360 pKa = 4.11PHH362 pKa = 8.7GPVQLSYY369 pKa = 11.75YY370 pKa = 10.41DD371 pKa = 3.38

Molecular weight: 40.47 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1P7U1E1|A0A1P7U1E1_EBVA8 Packaging protein UL32 OS=Epstein-Barr virus (strain AG876) OX=82830 PE=3 SV=1
MM1 pKa = 7.62ARR3 pKa = 11.84RR4 pKa = 11.84LPKK7 pKa = 9.12PTLQGRR13 pKa = 11.84LEE15 pKa = 4.27ADD17 pKa = 3.98FPDD20 pKa = 4.6SPLLPKK26 pKa = 10.41FQEE29 pKa = 4.16LNQNNLPNDD38 pKa = 3.73VFRR41 pKa = 11.84EE42 pKa = 4.15AQRR45 pKa = 11.84SYY47 pKa = 11.54LVFLTSQFCYY57 pKa = 10.33EE58 pKa = 4.52EE59 pKa = 4.17YY60 pKa = 9.89VQRR63 pKa = 11.84TFGVPRR69 pKa = 11.84RR70 pKa = 11.84QRR72 pKa = 11.84AIDD75 pKa = 3.21KK76 pKa = 9.86RR77 pKa = 11.84QRR79 pKa = 11.84ASVAGAGAHH88 pKa = 5.34AHH90 pKa = 6.49LGGSSATPVQQAQAAASAGTGALASSAPSTAVAQSATPSVSSSISSLRR138 pKa = 11.84AATSGATAAASAAAAVDD155 pKa = 3.72TGSGGGGQPQDD166 pKa = 3.46TAPRR170 pKa = 11.84GARR173 pKa = 11.84KK174 pKa = 9.13KK175 pKa = 8.65QQ176 pKa = 3.08

Molecular weight: 18.14 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

79

3

82

41182

60

3154

502.2

54.96

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.128 ± 0.413

1.969 ± 0.156

4.507 ± 0.153

5.434 ± 0.195

3.715 ± 0.168

7.418 ± 0.542

2.496 ± 0.134

3.723 ± 0.155

3.132 ± 0.158

10.497 ± 0.385

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.998 ± 0.119

3.052 ± 0.175

7.872 ± 0.433

3.943 ± 0.195

6.539 ± 0.243

7.71 ± 0.215

6.318 ± 0.374

6.486 ± 0.232

1.185 ± 0.078

2.875 ± 0.134
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski