Pseudarthrobacter phenanthrenivorans (strain DSM 18606 / JCM 16027 / LMG 23796 / Sphe3) (Arthrobacter phenanthrenivorans)

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Micrococcales; Micrococcaceae; Pseudarthrobacter; Pseudarthrobacter phenanthrenivorans

Average proteome isoelectric point is 6.18

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4081 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|F0M649|F0M649_PSEPM Probable 2-phosphosulfolactate phosphatase OS=Pseudarthrobacter phenanthrenivorans (strain DSM 18606 / JCM 16027 / LMG 23796 / Sphe3) OX=930171 GN=Asphe3_36040 PE=3 SV=1
MM1 pKa = 7.26SRR3 pKa = 11.84ALIIVDD9 pKa = 3.67VQNDD13 pKa = 3.63FCEE16 pKa = 4.91GGSLAVSGGADD27 pKa = 3.13VAGAISEE34 pKa = 4.37YY35 pKa = 11.07VEE37 pKa = 3.92AHH39 pKa = 6.25HH40 pKa = 7.22GEE42 pKa = 4.01FDD44 pKa = 5.04HH45 pKa = 7.71IVATQDD51 pKa = 2.81WHH53 pKa = 6.78IDD55 pKa = 3.48PGGHH59 pKa = 6.49FSEE62 pKa = 4.92TPDD65 pKa = 5.2FKK67 pKa = 11.34DD68 pKa = 2.73SWPPHH73 pKa = 5.87CVAGTPGAEE82 pKa = 4.06LHH84 pKa = 7.01PDD86 pKa = 3.48LDD88 pKa = 3.77TEE90 pKa = 4.48YY91 pKa = 10.49IQAYY95 pKa = 6.27FQKK98 pKa = 10.09GQYY101 pKa = 8.56AAAYY105 pKa = 9.73SGFEE109 pKa = 3.96GLLAPEE115 pKa = 4.76DD116 pKa = 3.76AVPTGEE122 pKa = 4.32RR123 pKa = 11.84QPGALPGPGDD133 pKa = 4.01AEE135 pKa = 4.03RR136 pKa = 11.84FAPDD140 pKa = 3.97DD141 pKa = 4.05DD142 pKa = 7.03AIGLDD147 pKa = 4.21DD148 pKa = 4.31WLQSHH153 pKa = 6.64DD154 pKa = 3.82VEE156 pKa = 4.63DD157 pKa = 4.06VVVVGIATDD166 pKa = 3.74YY167 pKa = 11.35CVMATALDD175 pKa = 4.0AVQAGYY181 pKa = 10.65SVTVLRR187 pKa = 11.84SLTAGIAEE195 pKa = 4.43DD196 pKa = 3.95LEE198 pKa = 4.29EE199 pKa = 4.82AVAEE203 pKa = 4.17MEE205 pKa = 4.47LGGVDD210 pKa = 3.52VAA212 pKa = 5.52

Molecular weight: 22.32 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|F0M2W7|F0M2W7_PSEPM Uncharacterized protein OS=Pseudarthrobacter phenanthrenivorans (strain DSM 18606 / JCM 16027 / LMG 23796 / Sphe3) OX=930171 GN=Asphe3_09340 PE=4 SV=1
MM1 pKa = 7.41NLGSMIRR8 pKa = 11.84NAAGRR13 pKa = 11.84FSGRR17 pKa = 11.84TGTSGTVRR25 pKa = 11.84GSGLNAPGRR34 pKa = 11.84TPGTTGAPGARR45 pKa = 11.84TGGVAGRR52 pKa = 11.84IMGMLRR58 pKa = 11.84RR59 pKa = 11.84RR60 pKa = 3.58

Molecular weight: 5.99 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4081

0

4081

1317772

26

2234

322.9

34.56

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.147 ± 0.055

0.623 ± 0.008

5.533 ± 0.034

5.727 ± 0.034

3.225 ± 0.024

9.186 ± 0.033

2.084 ± 0.02

4.26 ± 0.025

2.798 ± 0.026

10.255 ± 0.044

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.007 ± 0.016

2.476 ± 0.02

5.548 ± 0.03

3.213 ± 0.022

6.52 ± 0.038

5.824 ± 0.027

5.724 ± 0.029

8.301 ± 0.029

1.454 ± 0.016

2.095 ± 0.017
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski