Halonotius sp. J07HN4

Taxonomy: cellular organisms; Archaea; Euryarchaeota; Stenosarchaea group; Halobacteria; Haloferacales; Halorubraceae; Halonotius; unclassified Halonotius

Average proteome isoelectric point is 4.89

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3224 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|U1PA90|U1PA90_9EURY Uncharacterized protein OS=Halonotius sp. J07HN4 OX=1070774 GN=J07HN4v3_02535 PE=4 SV=1
MM1 pKa = 7.79ADD3 pKa = 3.72IDD5 pKa = 4.65LTLDD9 pKa = 3.13WTPNTNHH16 pKa = 6.31TGFYY20 pKa = 9.49VALAEE25 pKa = 5.32GYY27 pKa = 9.85YY28 pKa = 10.33ADD30 pKa = 4.93HH31 pKa = 6.73GLDD34 pKa = 3.62VAIHH38 pKa = 6.08SPAADD43 pKa = 4.03GYY45 pKa = 8.37EE46 pKa = 4.09TTPAKK51 pKa = 10.11QVATGEE57 pKa = 4.21ATLAIAPSEE66 pKa = 4.35SVISYY71 pKa = 7.52QTHH74 pKa = 6.79PDD76 pKa = 3.8YY77 pKa = 11.14PSLTAVAAVCQEE89 pKa = 4.17DD90 pKa = 3.51KK91 pKa = 11.32SAIVTLGDD99 pKa = 3.53SDD101 pKa = 4.83LDD103 pKa = 3.9RR104 pKa = 11.84PADD107 pKa = 3.99LDD109 pKa = 3.66GATYY113 pKa = 10.85ASYY116 pKa = 10.75DD117 pKa = 3.43ARR119 pKa = 11.84FEE121 pKa = 4.14DD122 pKa = 4.36HH123 pKa = 7.18IVRR126 pKa = 11.84QLVRR130 pKa = 11.84NDD132 pKa = 3.7GGDD135 pKa = 3.41GDD137 pKa = 4.43IEE139 pKa = 4.32IVTPPKK145 pKa = 10.41LGIWNTLLDD154 pKa = 4.27GDD156 pKa = 5.6ADD158 pKa = 4.08ATWVFMPWEE167 pKa = 4.49GVLAARR173 pKa = 11.84DD174 pKa = 4.57GIDD177 pKa = 3.42LTPFYY182 pKa = 10.64LDD184 pKa = 3.95TYY186 pKa = 9.83DD187 pKa = 3.37VPYY190 pKa = 10.74GYY192 pKa = 8.78TPLLLARR199 pKa = 11.84PEE201 pKa = 4.29TIADD205 pKa = 3.51GDD207 pKa = 3.98ALADD211 pKa = 4.06FLAATAHH218 pKa = 7.04GYY220 pKa = 9.28QFAVDD225 pKa = 3.8NPDD228 pKa = 3.14RR229 pKa = 11.84AAEE232 pKa = 4.06ILGEE236 pKa = 4.18TAEE239 pKa = 5.36GMDD242 pKa = 4.43NDD244 pKa = 4.93DD245 pKa = 4.01PDD247 pKa = 4.04FLRR250 pKa = 11.84EE251 pKa = 4.17SQHH254 pKa = 6.06EE255 pKa = 4.2LTDD258 pKa = 3.74AYY260 pKa = 10.05LTADD264 pKa = 4.01GQWGRR269 pKa = 11.84MAHH272 pKa = 6.14DD273 pKa = 3.22RR274 pKa = 11.84WDD276 pKa = 3.84AFVDD280 pKa = 3.53WLADD284 pKa = 3.59TDD286 pKa = 3.95ILTTVDD292 pKa = 4.12GDD294 pKa = 4.55PIPADD299 pKa = 3.89EE300 pKa = 5.63LPTDD304 pKa = 3.92DD305 pKa = 6.12LYY307 pKa = 11.41TNEE310 pKa = 4.96LLSDD314 pKa = 3.7SS315 pKa = 4.2

Molecular weight: 34.27 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|U1QMW0|U1QMW0_9EURY Metal-dependent carboxypeptidase OS=Halonotius sp. J07HN4 OX=1070774 GN=J07HN4v3_02543 PE=3 SV=1
MM1 pKa = 8.02SLMAAGVVLAAVGGDD16 pKa = 3.61HH17 pKa = 7.51LFGEE21 pKa = 5.03PPNRR25 pKa = 11.84LHH27 pKa = 6.87PVALFGRR34 pKa = 11.84LVGPLDD40 pKa = 4.11RR41 pKa = 11.84SWPAPRR47 pKa = 11.84LIGGVAALSLPLLAAGVVAVVVWIAGRR74 pKa = 11.84SHH76 pKa = 6.78PVVGSVVAAGVLFSTISLRR95 pKa = 11.84LLCATASNVLNQTEE109 pKa = 4.5TNLTDD114 pKa = 3.35ARR116 pKa = 11.84EE117 pKa = 4.12ALLALAGRR125 pKa = 11.84DD126 pKa = 3.72ATKK129 pKa = 10.79LSADD133 pKa = 3.84HH134 pKa = 6.17VRR136 pKa = 11.84SAAVEE141 pKa = 4.13SAAEE145 pKa = 3.97NLSDD149 pKa = 4.35GFVAPLVGFLIASVVGSRR167 pKa = 11.84WLTPGATLAVATAAAAWIKK186 pKa = 10.64AINTMDD192 pKa = 3.37SMVGYY197 pKa = 7.96RR198 pKa = 11.84TKK200 pKa = 10.65PVGWAAARR208 pKa = 11.84LDD210 pKa = 4.26DD211 pKa = 4.6LVMWVPARR219 pKa = 11.84LTAIAISVAALSPDD233 pKa = 3.39PLLVGRR239 pKa = 11.84RR240 pKa = 11.84WARR243 pKa = 11.84EE244 pKa = 3.67PASPNAGWPMATLSAALGVRR264 pKa = 11.84LDD266 pKa = 3.78KK267 pKa = 10.81PGHH270 pKa = 4.84YY271 pKa = 8.94TLNAVASLPTTAAGHH286 pKa = 6.12RR287 pKa = 11.84GVAIVRR293 pKa = 11.84RR294 pKa = 11.84AGWLTALSVAVIAGVV309 pKa = 3.08

Molecular weight: 31.64 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3224

0

3224

812135

25

1521

251.9

27.23

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

11.111 ± 0.056

0.77 ± 0.015

8.628 ± 0.056

8.047 ± 0.065

3.101 ± 0.029

8.223 ± 0.045

1.937 ± 0.025

4.826 ± 0.036

1.923 ± 0.026

8.73 ± 0.052

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.763 ± 0.019

2.533 ± 0.022

4.584 ± 0.029

2.876 ± 0.026

6.043 ± 0.041

5.676 ± 0.036

7.153 ± 0.038

8.379 ± 0.044

1.027 ± 0.018

2.54 ± 0.025
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski