Actinomycetospora cinnamomea
Average proteome isoelectric point is 6.18
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 5694 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A2U1F6R2|A0A2U1F6R2_9PSEU Uncharacterized protein OS=Actinomycetospora cinnamomea OX=663609 GN=C8D89_11026 PE=4 SV=1
MM1 pKa = 7.83 RR2 pKa = 11.84 GRR4 pKa = 11.84 RR5 pKa = 11.84 SSRR8 pKa = 11.84 SAAVVLAGCVGLAVMAPGLALAAPVPTSEE37 pKa = 4.94 DD38 pKa = 3.19 ACEE41 pKa = 3.89 AAGGRR46 pKa = 11.84 WTAGTCVPQRR56 pKa = 11.84 TAPLTTSPAVEE67 pKa = 4.72 DD68 pKa = 4.0 EE69 pKa = 4.47 TGGAATTPDD78 pKa = 3.91 TPEE81 pKa = 3.68 TAAPTTTTDD90 pKa = 3.53 GLPSGGNPSSGTPATGGGSGGAGGAVGAGAGAQEE124 pKa = 4.68 TPDD127 pKa = 3.62 PTADD131 pKa = 3.58 EE132 pKa = 4.83 ASASADD138 pKa = 3.14 QTTRR142 pKa = 11.84 SPEE145 pKa = 4.09 EE146 pKa = 3.86 IGTQQSTARR155 pKa = 11.84 ALIGPSALTSADD167 pKa = 3.36 PAALVQDD174 pKa = 4.35 LVEE177 pKa = 4.67 GASGPGIDD185 pKa = 4.32 FTNVPSPLTEE195 pKa = 4.55 GGLPFEE201 pKa = 4.37 NLEE204 pKa = 4.13 IPEE207 pKa = 4.57 LPADD211 pKa = 3.97 GVFASPRR218 pKa = 11.84 DD219 pKa = 3.47 ACAYY223 pKa = 10.12 LAGSVPAPEE232 pKa = 4.48 QGAEE236 pKa = 3.91 DD237 pKa = 3.46 LAAAFEE243 pKa = 4.7 SFCGALPEE251 pKa = 5.15 DD252 pKa = 4.08 FTEE255 pKa = 4.82 FDD257 pKa = 3.33 PTTIVGGLLDD267 pKa = 6.29 LINKK271 pKa = 8.39 LLKK274 pKa = 10.52 ALPTPGNPIPSNPPAHH290 pKa = 6.39 VPHH293 pKa = 7.25 EE294 pKa = 4.23 YY295 pKa = 9.5 WGWWHH300 pKa = 6.29 KK301 pKa = 10.96 QYY303 pKa = 11.38 DD304 pKa = 4.23 VNCDD308 pKa = 3.14 DD309 pKa = 3.55 VTYY312 pKa = 11.01 DD313 pKa = 3.4 EE314 pKa = 5.55 ANAILAWDD322 pKa = 3.71 YY323 pKa = 11.78 SDD325 pKa = 5.14 PFNLDD330 pKa = 2.8 GDD332 pKa = 4.36 NDD334 pKa = 4.08 GEE336 pKa = 4.1 ACEE339 pKa = 5.02 RR340 pKa = 11.84 NWHH343 pKa = 6.39 HH344 pKa = 7.68 DD345 pKa = 3.17 DD346 pKa = 3.6 VEE348 pKa = 4.25 YY349 pKa = 11.18 VEE351 pKa = 4.21 YY352 pKa = 10.92 VEE354 pKa = 4.04 YY355 pKa = 11.04 VEE357 pKa = 4.19 YY358 pKa = 11.08 VEE360 pKa = 4.23 YY361 pKa = 10.69 DD362 pKa = 3.48 SYY364 pKa = 11.7 PVGGVATGDD373 pKa = 3.47 GSTGADD379 pKa = 2.92 SSAATGAEE387 pKa = 3.99 VALAAGALSGLGLTGLVLVRR407 pKa = 11.84 RR408 pKa = 11.84 FARR411 pKa = 11.84 QGG413 pKa = 3.07
Molecular weight: 41.83 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.713
IPC2_protein 3.859
IPC_protein 3.859
Toseland 3.643
ProMoST 3.999
Dawson 3.834
Bjellqvist 3.986
Wikipedia 3.732
Rodwell 3.681
Grimsley 3.554
Solomon 3.821
Lehninger 3.783
Nozaki 3.935
DTASelect 4.139
Thurlkill 3.681
EMBOSS 3.745
Sillero 3.973
Patrickios 1.303
IPC_peptide 3.821
IPC2_peptide 3.948
IPC2.peptide.svr19 3.86
Protein with the highest isoelectric point:
>tr|A0A2U1FR97|A0A2U1FR97_9PSEU Methylmalonyl-CoA epimerase OS=Actinomycetospora cinnamomea OX=663609 GN=C8D89_101562 PE=3 SV=1
MM1 pKa = 7.74 PATAGRR7 pKa = 11.84 VPARR11 pKa = 11.84 PRR13 pKa = 11.84 VRR15 pKa = 11.84 GPAAARR21 pKa = 11.84 PSRR24 pKa = 11.84 ARR26 pKa = 11.84 PAARR30 pKa = 11.84 PVAPPAPVRR39 pKa = 11.84 PPARR43 pKa = 11.84 VPGRR47 pKa = 11.84 RR48 pKa = 11.84 PRR50 pKa = 11.84 PTARR54 pKa = 11.84 APAAPRR60 pKa = 11.84 VPAPVPAPPRR70 pKa = 11.84 ARR72 pKa = 11.84 VPAPSPARR80 pKa = 11.84 PPRR83 pKa = 11.84 RR84 pKa = 11.84 RR85 pKa = 11.84 RR86 pKa = 11.84 RR87 pKa = 11.84 PRR89 pKa = 11.84 PPRR92 pKa = 11.84 RR93 pKa = 11.84 RR94 pKa = 11.84 RR95 pKa = 11.84 LPRR98 pKa = 11.84 PVTPPAARR106 pKa = 11.84 RR107 pKa = 11.84 RR108 pKa = 11.84 AHH110 pKa = 6.9 RR111 pKa = 11.84 APAGTVPVPVATARR125 pKa = 11.84 VRR127 pKa = 11.84 VARR130 pKa = 11.84 RR131 pKa = 11.84 GPVPSVPPSAVATSARR147 pKa = 11.84 AEE149 pKa = 4.14 VEE151 pKa = 4.01 TAGTVVTARR160 pKa = 11.84 PRR162 pKa = 11.84 APVSSSSVRR171 pKa = 11.84 PRR173 pKa = 11.84 RR174 pKa = 11.84 RR175 pKa = 11.84 TQARR179 pKa = 11.84 AVTARR184 pKa = 11.84 RR185 pKa = 11.84 SPRR188 pKa = 11.84 ARR190 pKa = 11.84 RR191 pKa = 11.84 PRR193 pKa = 11.84 SPGRR197 pKa = 11.84 RR198 pKa = 11.84 PRR200 pKa = 11.84 ASATTPSGWAPAPRR214 pKa = 11.84 RR215 pKa = 11.84 ARR217 pKa = 11.84 SVRR220 pKa = 11.84 SRR222 pKa = 11.84 VRR224 pKa = 11.84 AARR227 pKa = 11.84 VRR229 pKa = 11.84 RR230 pKa = 11.84 VTARR234 pKa = 11.84 PAPTPRR240 pKa = 11.84 RR241 pKa = 11.84 ARR243 pKa = 11.84 RR244 pKa = 11.84 VTASVRR250 pKa = 11.84 RR251 pKa = 11.84 RR252 pKa = 11.84 RR253 pKa = 11.84 TACPVPAAPVVPGRR267 pKa = 11.84 RR268 pKa = 11.84 RR269 pKa = 11.84 RR270 pKa = 11.84 RR271 pKa = 11.84 ATCPRR276 pKa = 11.84 VRR278 pKa = 11.84 TPAA281 pKa = 3.42
Molecular weight: 30.42 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.521
IPC2_protein 11.389
IPC_protein 13.013
Toseland 13.173
ProMoST 13.671
Dawson 13.173
Bjellqvist 13.173
Wikipedia 13.656
Rodwell 12.676
Grimsley 13.217
Solomon 13.671
Lehninger 13.568
Nozaki 13.173
DTASelect 13.173
Thurlkill 13.173
EMBOSS 13.671
Sillero 13.173
Patrickios 12.369
IPC_peptide 13.671
IPC2_peptide 12.661
IPC2.peptide.svr19 9.317
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
5694
0
5694
1792867
27
4335
314.9
33.41
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
14.567 ± 0.058
0.728 ± 0.009
6.542 ± 0.027
5.646 ± 0.031
2.402 ± 0.017
9.841 ± 0.031
2.329 ± 0.017
2.65 ± 0.02
1.158 ± 0.019
10.272 ± 0.038
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.559 ± 0.012
1.264 ± 0.014
6.626 ± 0.037
2.287 ± 0.018
8.886 ± 0.033
4.534 ± 0.021
5.878 ± 0.023
9.724 ± 0.04
1.471 ± 0.013
1.636 ± 0.015
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here