Ostreococcus lucimarinus virus OlV3
Average proteome isoelectric point is 6.63
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 246 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|I3UL68|I3UL68_9PHYC RING-type domain-containing protein OS=Ostreococcus lucimarinus virus OlV3 OX=754062 GN=OMVG_00239 PE=4 SV=1
MM1 pKa = 7.4 IVDD4 pKa = 3.97 VQCEE8 pKa = 3.97 DD9 pKa = 3.69 DD10 pKa = 4.21 TIQIANLVRR19 pKa = 11.84 DD20 pKa = 4.25 EE21 pKa = 4.22 NTHH24 pKa = 5.33 DD25 pKa = 3.52 VEE27 pKa = 4.61 VRR29 pKa = 11.84 FLKK32 pKa = 10.5 RR33 pKa = 11.84 IKK35 pKa = 10.81 SNLYY39 pKa = 10.66 DD40 pKa = 3.86 FDD42 pKa = 4.93 NEE44 pKa = 3.77 ISVVPKK50 pKa = 10.36 EE51 pKa = 4.74 SVCGWYY57 pKa = 10.01 DD58 pKa = 3.66 CEE60 pKa = 4.13 NLEE63 pKa = 4.75 DD64 pKa = 3.71 TEE66 pKa = 5.76 LYY68 pKa = 10.61 VKK70 pKa = 10.87 VSGGYY75 pKa = 10.44 DD76 pKa = 3.22 LVDD79 pKa = 4.16 DD80 pKa = 5.48 SEE82 pKa = 6.45 DD83 pKa = 3.58 EE84 pKa = 5.27 DD85 pKa = 4.64 FTCSEE90 pKa = 3.97 SDD92 pKa = 3.6 EE93 pKa = 4.64 PDD95 pKa = 3.54 SEE97 pKa = 4.66 SEE99 pKa = 4.29 SLVDD103 pKa = 3.89 EE104 pKa = 5.27 DD105 pKa = 4.48 EE106 pKa = 4.2 AA107 pKa = 6.24
Molecular weight: 12.26 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.793
IPC2_protein 3.592
IPC_protein 3.579
Toseland 3.376
ProMoST 3.745
Dawson 3.579
Bjellqvist 3.732
Wikipedia 3.503
Rodwell 3.414
Grimsley 3.287
Solomon 3.554
Lehninger 3.516
Nozaki 3.694
DTASelect 3.897
Thurlkill 3.427
EMBOSS 3.516
Sillero 3.706
Patrickios 1.799
IPC_peptide 3.554
IPC2_peptide 3.681
IPC2.peptide.svr19 3.684
Protein with the highest isoelectric point:
>tr|I3UKQ3|I3UKQ3_9PHYC Uncharacterized protein OS=Ostreococcus lucimarinus virus OlV3 OX=754062 GN=OMVG_00066 PE=4 SV=1
MM1 pKa = 7.24 HH2 pKa = 6.92 VKK4 pKa = 10.19 ILRR7 pKa = 11.84 SPDD10 pKa = 2.97 RR11 pKa = 11.84 KK12 pKa = 10.34 KK13 pKa = 10.71 KK14 pKa = 10.13 FRR16 pKa = 11.84 AILGDD21 pKa = 3.19 GRR23 pKa = 11.84 TVDD26 pKa = 5.31 FGASGYY32 pKa = 10.89 SDD34 pKa = 3.53 YY35 pKa = 10.9 TKK37 pKa = 10.58 HH38 pKa = 6.49 KK39 pKa = 9.02 NPSRR43 pKa = 11.84 MRR45 pKa = 11.84 SYY47 pKa = 11.14 VLRR50 pKa = 11.84 HH51 pKa = 6.09 GGQIPKK57 pKa = 10.04 RR58 pKa = 11.84 VVAEE62 pKa = 4.3 RR63 pKa = 11.84 EE64 pKa = 3.5 PAMIHH69 pKa = 6.48 RR70 pKa = 11.84 MMRR73 pKa = 11.84 NIDD76 pKa = 3.27 KK77 pKa = 10.62 SDD79 pKa = 3.71 RR80 pKa = 11.84 EE81 pKa = 4.1 DD82 pKa = 3.09 WKK84 pKa = 11.38 LSGIGGAGFWSRR96 pKa = 11.84 WYY98 pKa = 10.29 LWSQPTIPAVQRR110 pKa = 11.84 FMSKK114 pKa = 9.89 RR115 pKa = 11.84 FGIKK119 pKa = 10.36 FII121 pKa = 4.63
Molecular weight: 14.18 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.296
IPC2_protein 9.999
IPC_protein 10.979
Toseland 11.155
ProMoST 11.155
Dawson 11.213
Bjellqvist 10.994
Wikipedia 11.506
Rodwell 11.374
Grimsley 11.242
Solomon 11.462
Lehninger 11.403
Nozaki 11.14
DTASelect 10.994
Thurlkill 11.155
EMBOSS 11.594
Sillero 11.155
Patrickios 11.096
IPC_peptide 11.477
IPC2_peptide 10.087
IPC2.peptide.svr19 8.528
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
246
0
246
55301
62
2651
224.8
25.57
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
5.763 ± 0.236
1.609 ± 0.126
5.893 ± 0.182
6.824 ± 0.229
4.249 ± 0.138
6.105 ± 0.305
2.21 ± 0.12
6.4 ± 0.167
7.821 ± 0.339
8.058 ± 0.177
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.919 ± 0.124
5.922 ± 0.421
4.027 ± 0.15
3.349 ± 0.13
5.081 ± 0.233
6.032 ± 0.162
6.183 ± 0.294
6.488 ± 0.189
1.072 ± 0.077
3.995 ± 0.177
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here