Amino acid dipepetide frequency for Vibrio phage P23

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
6.984AlaAla: 6.984 ± 1.184
0.575AlaCys: 0.575 ± 0.25
3.862AlaAsp: 3.862 ± 0.544
4.93AlaGlu: 4.93 ± 0.702
3.451AlaPhe: 3.451 ± 0.624
4.848AlaGly: 4.848 ± 0.73
1.15AlaHis: 1.15 ± 0.277
6.738AlaIle: 6.738 ± 0.927
5.423AlaLys: 5.423 ± 0.652
6.82AlaLeu: 6.82 ± 0.718
3.205AlaMet: 3.205 ± 0.789
3.698AlaAsn: 3.698 ± 0.574
2.136AlaPro: 2.136 ± 0.494
3.205AlaGln: 3.205 ± 0.881
3.78AlaArg: 3.78 ± 0.701
4.684AlaSer: 4.684 ± 0.655
4.437AlaThr: 4.437 ± 1.053
4.273AlaVal: 4.273 ± 0.544
0.74AlaTrp: 0.74 ± 0.267
2.301AlaTyr: 2.301 ± 0.438
0.0AlaXaa: 0.0 ± 0.0
Cys
0.575CysAla: 0.575 ± 0.216
0.164CysCys: 0.164 ± 0.133
1.068CysAsp: 1.068 ± 0.41
1.643CysGlu: 1.643 ± 0.412
0.493CysPhe: 0.493 ± 0.184
1.15CysGly: 1.15 ± 0.309
0.247CysHis: 0.247 ± 0.155
0.986CysIle: 0.986 ± 0.299
1.068CysLys: 1.068 ± 0.263
1.479CysLeu: 1.479 ± 0.354
0.082CysMet: 0.082 ± 0.082
0.575CysAsn: 0.575 ± 0.226
0.411CysPro: 0.411 ± 0.19
0.904CysGln: 0.904 ± 0.333
0.904CysArg: 0.904 ± 0.346
0.411CysSer: 0.411 ± 0.207
0.575CysThr: 0.575 ± 0.222
1.233CysVal: 1.233 ± 0.313
0.247CysTrp: 0.247 ± 0.138
0.493CysTyr: 0.493 ± 0.214
0.0CysXaa: 0.0 ± 0.0
Asp
5.259AspAla: 5.259 ± 0.632
1.068AspCys: 1.068 ± 0.255
5.012AspAsp: 5.012 ± 0.692
4.601AspGlu: 4.601 ± 0.654
3.287AspPhe: 3.287 ± 0.596
5.012AspGly: 5.012 ± 0.788
0.986AspHis: 0.986 ± 0.34
4.437AspIle: 4.437 ± 0.558
4.93AspLys: 4.93 ± 0.599
4.601AspLeu: 4.601 ± 0.645
1.561AspMet: 1.561 ± 0.392
2.794AspAsn: 2.794 ± 0.435
1.726AspPro: 1.726 ± 0.351
1.972AspGln: 1.972 ± 0.41
1.561AspArg: 1.561 ± 0.427
4.026AspSer: 4.026 ± 0.527
2.794AspThr: 2.794 ± 0.524
3.78AspVal: 3.78 ± 0.536
1.068AspTrp: 1.068 ± 0.479
2.629AspTyr: 2.629 ± 0.43
0.0AspXaa: 0.0 ± 0.0
Glu
5.341GluAla: 5.341 ± 0.858
0.904GluCys: 0.904 ± 0.325
2.465GluAsp: 2.465 ± 0.416
4.273GluGlu: 4.273 ± 0.732
3.78GluPhe: 3.78 ± 0.506
3.615GluGly: 3.615 ± 0.499
1.561GluHis: 1.561 ± 0.402
3.944GluIle: 3.944 ± 0.66
4.766GluLys: 4.766 ± 0.711
7.477GluLeu: 7.477 ± 0.847
2.547GluMet: 2.547 ± 0.456
4.273GluAsn: 4.273 ± 0.571
1.726GluPro: 1.726 ± 0.402
3.205GluGln: 3.205 ± 0.621
2.876GluArg: 2.876 ± 0.457
4.355GluSer: 4.355 ± 0.63
3.698GluThr: 3.698 ± 0.495
5.423GluVal: 5.423 ± 0.708
1.068GluTrp: 1.068 ± 0.3
3.04GluTyr: 3.04 ± 0.529
0.0GluXaa: 0.0 ± 0.0
Phe
2.383PheAla: 2.383 ± 0.418
0.986PheCys: 0.986 ± 0.337
3.369PheAsp: 3.369 ± 0.564
2.876PheGlu: 2.876 ± 0.554
1.15PhePhe: 1.15 ± 0.303
3.533PheGly: 3.533 ± 0.628
0.822PheHis: 0.822 ± 0.293
2.465PheIle: 2.465 ± 0.554
2.712PheLys: 2.712 ± 0.428
2.465PheLeu: 2.465 ± 0.411
0.657PheMet: 0.657 ± 0.229
2.958PheAsn: 2.958 ± 0.499
0.74PhePro: 0.74 ± 0.236
1.479PheGln: 1.479 ± 0.375
1.315PheArg: 1.315 ± 0.28
3.615PheSer: 3.615 ± 0.403
2.629PheThr: 2.629 ± 0.601
2.465PheVal: 2.465 ± 0.646
0.657PheTrp: 0.657 ± 0.275
2.054PheTyr: 2.054 ± 0.43
0.0PheXaa: 0.0 ± 0.0
Gly
4.684GlyAla: 4.684 ± 0.675
1.233GlyCys: 1.233 ± 0.338
4.601GlyAsp: 4.601 ± 0.643
4.519GlyGlu: 4.519 ± 0.569
3.78GlyPhe: 3.78 ± 0.671
5.67GlyGly: 5.67 ± 0.746
0.657GlyHis: 0.657 ± 0.221
4.601GlyIle: 4.601 ± 0.575
4.766GlyLys: 4.766 ± 0.73
5.834GlyLeu: 5.834 ± 0.794
1.972GlyMet: 1.972 ± 0.507
3.944GlyAsn: 3.944 ± 0.455
0.493GlyPro: 0.493 ± 0.193
2.794GlyGln: 2.794 ± 0.681
2.383GlyArg: 2.383 ± 0.374
4.026GlySer: 4.026 ± 0.637
4.191GlyThr: 4.191 ± 0.792
4.766GlyVal: 4.766 ± 0.709
1.315GlyTrp: 1.315 ± 0.327
2.794GlyTyr: 2.794 ± 0.487
0.0GlyXaa: 0.0 ± 0.0
His
1.643HisAla: 1.643 ± 0.422
0.247HisCys: 0.247 ± 0.137
1.068HisAsp: 1.068 ± 0.356
1.068HisGlu: 1.068 ± 0.329
0.657HisPhe: 0.657 ± 0.235
1.068HisGly: 1.068 ± 0.267
0.329HisHis: 0.329 ± 0.178
1.233HisIle: 1.233 ± 0.32
0.904HisLys: 0.904 ± 0.421
1.15HisLeu: 1.15 ± 0.291
0.411HisMet: 0.411 ± 0.203
0.657HisAsn: 0.657 ± 0.288
0.986HisPro: 0.986 ± 0.33
0.493HisGln: 0.493 ± 0.187
0.822HisArg: 0.822 ± 0.241
0.74HisSer: 0.74 ± 0.305
0.575HisThr: 0.575 ± 0.225
0.904HisVal: 0.904 ± 0.359
0.822HisTrp: 0.822 ± 0.269
1.15HisTyr: 1.15 ± 0.335
0.0HisXaa: 0.0 ± 0.0
Ile
4.601IleAla: 4.601 ± 0.608
1.397IleCys: 1.397 ± 0.375
5.998IleAsp: 5.998 ± 0.71
5.423IleGlu: 5.423 ± 0.706
1.397IlePhe: 1.397 ± 0.36
4.601IleGly: 4.601 ± 0.635
1.068IleHis: 1.068 ± 0.322
2.301IleIle: 2.301 ± 0.398
5.588IleLys: 5.588 ± 0.804
3.944IleLeu: 3.944 ± 0.511
1.643IleMet: 1.643 ± 0.344
3.451IleAsn: 3.451 ± 0.54
2.301IlePro: 2.301 ± 0.437
1.808IleGln: 1.808 ± 0.375
2.794IleArg: 2.794 ± 0.477
3.122IleSer: 3.122 ± 0.425
3.862IleThr: 3.862 ± 0.487
3.04IleVal: 3.04 ± 0.47
0.411IleTrp: 0.411 ± 0.194
2.301IleTyr: 2.301 ± 0.427
0.0IleXaa: 0.0 ± 0.0
Lys
5.67LysAla: 5.67 ± 0.593
0.986LysCys: 0.986 ± 0.297
4.355LysAsp: 4.355 ± 0.623
5.505LysGlu: 5.505 ± 0.785
2.958LysPhe: 2.958 ± 0.589
3.533LysGly: 3.533 ± 0.458
0.986LysHis: 0.986 ± 0.329
3.944LysIle: 3.944 ± 0.544
5.834LysLys: 5.834 ± 0.818
6.656LysLeu: 6.656 ± 0.731
2.547LysMet: 2.547 ± 0.442
3.287LysAsn: 3.287 ± 0.478
2.465LysPro: 2.465 ± 0.543
2.547LysGln: 2.547 ± 0.444
3.615LysArg: 3.615 ± 0.64
5.341LysSer: 5.341 ± 0.647
3.944LysThr: 3.944 ± 0.398
5.259LysVal: 5.259 ± 0.565
1.233LysTrp: 1.233 ± 0.333
2.958LysTyr: 2.958 ± 0.742
0.0LysXaa: 0.0 ± 0.0
Leu
6.245LeuAla: 6.245 ± 1.064
1.643LeuCys: 1.643 ± 0.463
4.93LeuAsp: 4.93 ± 0.812
6.081LeuGlu: 6.081 ± 0.732
2.301LeuPhe: 2.301 ± 0.454
5.998LeuGly: 5.998 ± 1.014
1.643LeuHis: 1.643 ± 0.49
5.094LeuIle: 5.094 ± 0.909
5.998LeuLys: 5.998 ± 0.699
4.93LeuLeu: 4.93 ± 0.545
2.301LeuMet: 2.301 ± 0.38
4.273LeuAsn: 4.273 ± 0.571
2.876LeuPro: 2.876 ± 0.532
2.054LeuGln: 2.054 ± 0.434
3.78LeuArg: 3.78 ± 0.55
6.163LeuSer: 6.163 ± 0.787
5.588LeuThr: 5.588 ± 0.753
4.108LeuVal: 4.108 ± 0.609
0.986LeuTrp: 0.986 ± 0.287
1.89LeuTyr: 1.89 ± 0.41
0.0LeuXaa: 0.0 ± 0.0
Met
3.287MetAla: 3.287 ± 0.795
0.411MetCys: 0.411 ± 0.183
1.643MetAsp: 1.643 ± 0.408
1.397MetGlu: 1.397 ± 0.319
1.561MetPhe: 1.561 ± 0.312
1.972MetGly: 1.972 ± 0.506
0.493MetHis: 0.493 ± 0.207
2.054MetIle: 2.054 ± 0.353
2.136MetLys: 2.136 ± 0.566
1.808MetLeu: 1.808 ± 0.422
0.329MetMet: 0.329 ± 0.152
1.233MetAsn: 1.233 ± 0.34
0.986MetPro: 0.986 ± 0.309
1.397MetGln: 1.397 ± 0.321
1.233MetArg: 1.233 ± 0.394
3.04MetSer: 3.04 ± 0.558
1.972MetThr: 1.972 ± 0.402
1.643MetVal: 1.643 ± 0.397
0.329MetTrp: 0.329 ± 0.167
0.822MetTyr: 0.822 ± 0.295
0.0MetXaa: 0.0 ± 0.0
Asn
4.108AsnAla: 4.108 ± 0.553
0.74AsnCys: 0.74 ± 0.273
3.287AsnAsp: 3.287 ± 0.551
3.451AsnGlu: 3.451 ± 0.586
1.972AsnPhe: 1.972 ± 0.518
4.519AsnGly: 4.519 ± 0.722
1.15AsnHis: 1.15 ± 0.309
2.465AsnIle: 2.465 ± 0.496
3.615AsnLys: 3.615 ± 0.54
4.108AsnLeu: 4.108 ± 0.604
1.233AsnMet: 1.233 ± 0.284
3.287AsnAsn: 3.287 ± 0.44
2.547AsnPro: 2.547 ± 0.498
2.547AsnGln: 2.547 ± 0.514
1.808AsnArg: 1.808 ± 0.386
3.862AsnSer: 3.862 ± 0.626
3.205AsnThr: 3.205 ± 0.531
2.629AsnVal: 2.629 ± 0.532
0.904AsnTrp: 0.904 ± 0.275
2.136AsnTyr: 2.136 ± 0.365
0.0AsnXaa: 0.0 ± 0.0
Pro
1.972ProAla: 1.972 ± 0.398
0.411ProCys: 0.411 ± 0.179
2.136ProAsp: 2.136 ± 0.474
2.629ProGlu: 2.629 ± 0.591
1.561ProPhe: 1.561 ± 0.402
1.15ProGly: 1.15 ± 0.419
0.74ProHis: 0.74 ± 0.266
1.397ProIle: 1.397 ± 0.39
2.383ProLys: 2.383 ± 0.501
1.726ProLeu: 1.726 ± 0.309
0.904ProMet: 0.904 ± 0.312
1.479ProAsn: 1.479 ± 0.351
0.74ProPro: 0.74 ± 0.243
1.479ProGln: 1.479 ± 0.379
1.068ProArg: 1.068 ± 0.31
1.89ProSer: 1.89 ± 0.343
2.465ProThr: 2.465 ± 0.555
2.465ProVal: 2.465 ± 0.556
0.411ProTrp: 0.411 ± 0.208
1.726ProTyr: 1.726 ± 0.308
0.0ProXaa: 0.0 ± 0.0
Gln
3.287GlnAla: 3.287 ± 0.846
0.575GlnCys: 0.575 ± 0.196
2.547GlnAsp: 2.547 ± 0.48
2.547GlnGlu: 2.547 ± 0.549
1.561GlnPhe: 1.561 ± 0.333
2.465GlnGly: 2.465 ± 0.582
0.329GlnHis: 0.329 ± 0.22
3.369GlnIle: 3.369 ± 0.502
2.383GlnLys: 2.383 ± 0.384
3.122GlnLeu: 3.122 ± 0.65
1.643GlnMet: 1.643 ± 0.379
1.726GlnAsn: 1.726 ± 0.353
1.068GlnPro: 1.068 ± 0.314
3.04GlnGln: 3.04 ± 1.061
1.315GlnArg: 1.315 ± 0.322
2.465GlnSer: 2.465 ± 0.465
2.054GlnThr: 2.054 ± 0.46
2.219GlnVal: 2.219 ± 0.424
0.657GlnTrp: 0.657 ± 0.242
1.15GlnTyr: 1.15 ± 0.305
0.0GlnXaa: 0.0 ± 0.0
Arg
3.287ArgAla: 3.287 ± 0.558
0.575ArgCys: 0.575 ± 0.238
1.972ArgAsp: 1.972 ± 0.404
3.451ArgGlu: 3.451 ± 0.542
2.054ArgPhe: 2.054 ± 0.464
2.054ArgGly: 2.054 ± 0.394
0.493ArgHis: 0.493 ± 0.261
2.629ArgIle: 2.629 ± 0.554
3.533ArgLys: 3.533 ± 0.595
4.026ArgLeu: 4.026 ± 0.517
2.219ArgMet: 2.219 ± 0.507
2.629ArgAsn: 2.629 ± 0.465
1.315ArgPro: 1.315 ± 0.356
2.383ArgGln: 2.383 ± 0.446
1.89ArgArg: 1.89 ± 0.369
2.054ArgSer: 2.054 ± 0.487
1.972ArgThr: 1.972 ± 0.421
2.301ArgVal: 2.301 ± 0.538
0.247ArgTrp: 0.247 ± 0.139
0.904ArgTyr: 0.904 ± 0.332
0.0ArgXaa: 0.0 ± 0.0
Ser
4.601SerAla: 4.601 ± 1.004
1.15SerCys: 1.15 ± 0.319
4.355SerAsp: 4.355 ± 0.655
4.848SerGlu: 4.848 ± 0.707
2.629SerPhe: 2.629 ± 0.476
5.505SerGly: 5.505 ± 0.631
1.15SerHis: 1.15 ± 0.267
3.78SerIle: 3.78 ± 0.457
5.588SerLys: 5.588 ± 0.656
4.93SerLeu: 4.93 ± 0.638
2.054SerMet: 2.054 ± 0.446
2.794SerAsn: 2.794 ± 0.492
2.301SerPro: 2.301 ± 0.436
2.465SerGln: 2.465 ± 0.369
2.794SerArg: 2.794 ± 0.556
4.273SerSer: 4.273 ± 0.579
3.533SerThr: 3.533 ± 0.574
4.684SerVal: 4.684 ± 0.65
1.233SerTrp: 1.233 ± 0.348
2.794SerTyr: 2.794 ± 0.512
0.0SerXaa: 0.0 ± 0.0
Thr
4.684ThrAla: 4.684 ± 0.873
0.493ThrCys: 0.493 ± 0.25
4.191ThrAsp: 4.191 ± 0.562
4.108ThrGlu: 4.108 ± 0.645
1.726ThrPhe: 1.726 ± 0.466
4.684ThrGly: 4.684 ± 0.823
0.74ThrHis: 0.74 ± 0.256
3.451ThrIle: 3.451 ± 0.604
3.369ThrLys: 3.369 ± 0.499
4.519ThrLeu: 4.519 ± 0.647
1.15ThrMet: 1.15 ± 0.417
2.712ThrAsn: 2.712 ± 0.43
2.958ThrPro: 2.958 ± 0.506
2.054ThrGln: 2.054 ± 0.455
1.808ThrArg: 1.808 ± 0.295
3.862ThrSer: 3.862 ± 0.721
3.451ThrThr: 3.451 ± 0.692
3.78ThrVal: 3.78 ± 0.579
0.986ThrTrp: 0.986 ± 0.336
2.136ThrTyr: 2.136 ± 0.579
0.0ThrXaa: 0.0 ± 0.0
Val
5.094ValAla: 5.094 ± 0.728
0.493ValCys: 0.493 ± 0.218
3.698ValAsp: 3.698 ± 0.51
3.78ValGlu: 3.78 ± 0.448
2.629ValPhe: 2.629 ± 0.515
4.601ValGly: 4.601 ± 0.728
0.74ValHis: 0.74 ± 0.263
3.862ValIle: 3.862 ± 0.491
4.848ValLys: 4.848 ± 0.696
5.012ValLeu: 5.012 ± 0.546
2.136ValMet: 2.136 ± 0.38
4.766ValAsn: 4.766 ± 0.613
1.15ValPro: 1.15 ± 0.285
1.315ValGln: 1.315 ± 0.331
2.547ValArg: 2.547 ± 0.412
6.163ValSer: 6.163 ± 0.738
3.862ValThr: 3.862 ± 0.57
4.355ValVal: 4.355 ± 0.638
0.493ValTrp: 0.493 ± 0.211
1.643ValTyr: 1.643 ± 0.494
0.0ValXaa: 0.0 ± 0.0
Trp
0.904TrpAla: 0.904 ± 0.229
0.329TrpCys: 0.329 ± 0.167
0.575TrpAsp: 0.575 ± 0.245
0.986TrpGlu: 0.986 ± 0.33
0.822TrpPhe: 0.822 ± 0.279
0.575TrpGly: 0.575 ± 0.267
0.657TrpHis: 0.657 ± 0.254
0.657TrpIle: 0.657 ± 0.254
1.15TrpLys: 1.15 ± 0.367
1.726TrpLeu: 1.726 ± 0.391
0.329TrpMet: 0.329 ± 0.164
1.068TrpAsn: 1.068 ± 0.271
0.329TrpPro: 0.329 ± 0.195
0.575TrpGln: 0.575 ± 0.22
1.15TrpArg: 1.15 ± 0.314
0.904TrpSer: 0.904 ± 0.327
0.247TrpThr: 0.247 ± 0.141
0.575TrpVal: 0.575 ± 0.216
0.247TrpTrp: 0.247 ± 0.148
0.411TrpTyr: 0.411 ± 0.198
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.629TyrAla: 2.629 ± 0.389
0.329TyrCys: 0.329 ± 0.169
1.972TyrAsp: 1.972 ± 0.554
2.219TyrGlu: 2.219 ± 0.439
1.561TyrPhe: 1.561 ± 0.349
2.465TyrGly: 2.465 ± 0.521
0.986TyrHis: 0.986 ± 0.308
1.643TyrIle: 1.643 ± 0.494
2.629TyrLys: 2.629 ± 0.528
2.547TyrLeu: 2.547 ± 0.618
0.74TyrMet: 0.74 ± 0.241
1.972TyrAsn: 1.972 ± 0.335
1.479TyrPro: 1.479 ± 0.344
1.808TyrGln: 1.808 ± 0.374
2.547TyrArg: 2.547 ± 0.453
2.465TyrSer: 2.465 ± 0.405
1.808TyrThr: 1.808 ± 0.332
3.369TyrVal: 3.369 ± 0.708
0.164TyrTrp: 0.164 ± 0.117
1.15TyrTyr: 1.15 ± 0.374
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 72 proteins (12171 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski