Phage NC-G

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Myoviridae; Tevenvirinae; Dhakavirus; unclassified Dhakavirus

Average proteome isoelectric point is 6.1

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 275 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A482JTX9|A0A482JTX9_9CAUD Baseplate OS=Phage NC-G OX=2528530 PE=4 SV=1
MM1 pKa = 7.65LYY3 pKa = 10.55DD4 pKa = 4.35PNGCSEE10 pKa = 4.05NGIIVLFADD19 pKa = 4.71NKK21 pKa = 11.18DD22 pKa = 3.36SDD24 pKa = 4.69DD25 pKa = 4.05DD26 pKa = 4.42VFVTEE31 pKa = 4.74WDD33 pKa = 3.62CHH35 pKa = 7.03GGITLQQEE43 pKa = 3.95NDD45 pKa = 4.4MICLDD50 pKa = 3.73ASQVEE55 pKa = 4.32EE56 pKa = 4.74LYY58 pKa = 11.11SILKK62 pKa = 9.82HH63 pKa = 5.91NRR65 pKa = 2.92

Molecular weight: 7.38 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A482JSS3|A0A482JSS3_9CAUD Single stranded DNA-binding protein OS=Phage NC-G OX=2528530 PE=4 SV=1
MM1 pKa = 7.53AKK3 pKa = 10.16KK4 pKa = 9.46EE5 pKa = 4.17VKK7 pKa = 10.55VVDD10 pKa = 4.16GTSKK14 pKa = 9.78RR15 pKa = 11.84AGYY18 pKa = 9.8KK19 pKa = 9.18RR20 pKa = 11.84ASNKK24 pKa = 10.11RR25 pKa = 11.84INQVVDD31 pKa = 3.24KK32 pKa = 10.27LQARR36 pKa = 11.84ARR38 pKa = 11.84AVLRR42 pKa = 11.84ADD44 pKa = 3.76AACFGKK50 pKa = 10.89VIAA53 pKa = 5.26

Molecular weight: 5.79 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

273

2

275

53411

32

1322

194.2

21.98

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.077 ± 0.189

1.024 ± 0.063

6.428 ± 0.11

7.242 ± 0.157

4.349 ± 0.127

6.278 ± 0.196

1.693 ± 0.068

6.806 ± 0.111

7.502 ± 0.192

7.693 ± 0.137

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.788 ± 0.092

5.332 ± 0.12

3.643 ± 0.088

3.467 ± 0.1

4.274 ± 0.099

6.184 ± 0.126

5.795 ± 0.274

6.746 ± 0.119

1.404 ± 0.066

4.274 ± 0.124
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski