Nocardioides sp. (strain ATCC BAA-499 / JS614)

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Propionibacteriales; Nocardioidaceae; Nocardioides; unclassified Nocardioides

Average proteome isoelectric point is 6.02

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4888 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A1SK70|A1SK70_NOCSJ Putative exonuclease OS=Nocardioides sp. (strain ATCC BAA-499 / JS614) OX=196162 GN=Noca_2702 PE=4 SV=1
MM1 pKa = 7.5SLRR4 pKa = 11.84SAAAVASLATVALLLGGCGSDD25 pKa = 5.09DD26 pKa = 4.42GADD29 pKa = 3.31QKK31 pKa = 11.56SATDD35 pKa = 3.47AGATTSCTYY44 pKa = 10.79AADD47 pKa = 4.0GSAPAKK53 pKa = 10.29EE54 pKa = 4.2VDD56 pKa = 4.45PPAEE60 pKa = 4.48DD61 pKa = 3.98APASGTVALTMATSVGDD78 pKa = 3.98LGLTLDD84 pKa = 4.6AAAAPCTVNSFVSLAEE100 pKa = 3.54QGYY103 pKa = 10.28FDD105 pKa = 4.75DD106 pKa = 4.74TSCHH110 pKa = 6.71RR111 pKa = 11.84LTTQGIHH118 pKa = 5.87VLQCGDD124 pKa = 3.47PTGTGTGGPGYY135 pKa = 8.92TIQDD139 pKa = 3.56EE140 pKa = 4.68VDD142 pKa = 3.34GSEE145 pKa = 4.47SYY147 pKa = 10.36PAGTLAMARR156 pKa = 11.84TQAPDD161 pKa = 2.92SGGSQFFIVYY171 pKa = 10.01GDD173 pKa = 3.73TPLPPDD179 pKa = 3.38YY180 pKa = 10.62TVFGTVDD187 pKa = 3.48DD188 pKa = 5.23AGLKK192 pKa = 10.33AIEE195 pKa = 4.33KK196 pKa = 9.74VAGAGTDD203 pKa = 3.57EE204 pKa = 4.76ANGPGDD210 pKa = 3.97GHH212 pKa = 7.29PLTPVDD218 pKa = 3.45IRR220 pKa = 11.84SVTVGG225 pKa = 3.12

Molecular weight: 22.24 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>sp|A1SE34|NUOH_NOCSJ NADH-quinone oxidoreductase subunit H OS=Nocardioides sp. (strain ATCC BAA-499 / JS614) OX=196162 GN=nuoH PE=3 SV=1
MM1 pKa = 7.4GSVIKK6 pKa = 10.42KK7 pKa = 8.47RR8 pKa = 11.84RR9 pKa = 11.84KK10 pKa = 9.22RR11 pKa = 11.84MAKK14 pKa = 9.41KK15 pKa = 9.99KK16 pKa = 9.77HH17 pKa = 5.81RR18 pKa = 11.84KK19 pKa = 8.51LLKK22 pKa = 8.15KK23 pKa = 9.24TRR25 pKa = 11.84VQRR28 pKa = 11.84RR29 pKa = 11.84KK30 pKa = 10.07LGKK33 pKa = 9.87

Molecular weight: 4.07 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4888

0

4888

1591492

33

1980

325.6

34.89

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.272 ± 0.047

0.756 ± 0.008

6.398 ± 0.027

5.78 ± 0.034

2.768 ± 0.021

9.262 ± 0.032

2.279 ± 0.016

3.476 ± 0.025

1.847 ± 0.022

10.304 ± 0.04

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.84 ± 0.013

1.745 ± 0.017

5.654 ± 0.025

2.772 ± 0.018

7.913 ± 0.042

5.114 ± 0.023

5.998 ± 0.026

9.318 ± 0.033

1.521 ± 0.015

1.983 ± 0.014
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski