Thermobispora bispora (strain ATCC 19993 / DSM 43833 / CBS 139.67 / JCM 10125 / KCTC 9307 / NBRC 14880 / R51)

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Streptosporangiales; Streptosporangiaceae; Thermobispora; Thermobispora bispora

Average proteome isoelectric point is 6.78

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3545 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|D6YBB3|D6YBB3_THEBD Methyltransferase type 11 OS=Thermobispora bispora (strain ATCC 19993 / DSM 43833 / CBS 139.67 / JCM 10125 / KCTC 9307 / NBRC 14880 / R51) OX=469371 GN=Tbis_1760 PE=4 SV=1
MM1 pKa = 7.8KK2 pKa = 9.39ITVDD6 pKa = 3.34MSLCQDD12 pKa = 3.88HH13 pKa = 6.38GQCAIAAPAVFQMNEE28 pKa = 3.83EE29 pKa = 4.06GKK31 pKa = 10.32LVYY34 pKa = 9.54EE35 pKa = 4.63ANPDD39 pKa = 3.36DD40 pKa = 4.13SLRR43 pKa = 11.84EE44 pKa = 4.05EE45 pKa = 4.44VEE47 pKa = 4.07QAADD51 pKa = 3.55VCPVQAIFIEE61 pKa = 4.64DD62 pKa = 3.17

Molecular weight: 6.83 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|D6YB11|D6YB11_THEBD Binding-protein-dependent transport systems inner membrane component OS=Thermobispora bispora (strain ATCC 19993 / DSM 43833 / CBS 139.67 / JCM 10125 / KCTC 9307 / NBRC 14880 / R51) OX=469371 GN=Tbis_1658 PE=3 SV=1
MM1 pKa = 7.62TKK3 pKa = 9.09RR4 pKa = 11.84TYY6 pKa = 10.32QPNNRR11 pKa = 11.84RR12 pKa = 11.84RR13 pKa = 11.84AKK15 pKa = 8.7THH17 pKa = 5.15GFRR20 pKa = 11.84LRR22 pKa = 11.84MRR24 pKa = 11.84TRR26 pKa = 11.84AGRR29 pKa = 11.84AILAARR35 pKa = 11.84RR36 pKa = 11.84RR37 pKa = 11.84KK38 pKa = 9.45GRR40 pKa = 11.84ARR42 pKa = 11.84LTVV45 pKa = 3.04

Molecular weight: 5.41 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3545

0

3545

1161504

30

1807

327.6

35.33

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.268 ± 0.062

0.837 ± 0.011

5.372 ± 0.033

6.218 ± 0.042

2.781 ± 0.024

9.265 ± 0.039

2.077 ± 0.02

4.224 ± 0.026

2.206 ± 0.028

10.326 ± 0.046

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.813 ± 0.017

1.702 ± 0.021

6.392 ± 0.038

2.459 ± 0.018

8.866 ± 0.046

4.511 ± 0.028

5.524 ± 0.029

8.54 ± 0.032

1.476 ± 0.016

2.145 ± 0.019
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski