Ewingella americana ATCC 33852

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales; Yersiniaceae; Ewingella; Ewingella americana

Average proteome isoelectric point is 6.44

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4395 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A085G6A0|A0A085G6A0_9GAMM HTH-type transcriptional regulator ArgP OS=Ewingella americana ATCC 33852 OX=910964 GN=argP PE=3 SV=1
MM1 pKa = 7.42SNVPSEE7 pKa = 4.39LKK9 pKa = 10.53YY10 pKa = 10.37AASHH14 pKa = 5.0EE15 pKa = 4.52WVRR18 pKa = 11.84SEE20 pKa = 4.01GNGVYY25 pKa = 9.34TVGITEE31 pKa = 4.48HH32 pKa = 6.19AQEE35 pKa = 4.67LLGDD39 pKa = 3.97MVFVDD44 pKa = 4.72LPEE47 pKa = 4.27VGRR50 pKa = 11.84QVAAGEE56 pKa = 4.18DD57 pKa = 3.85CAVAEE62 pKa = 4.4SVKK65 pKa = 10.43AASDD69 pKa = 3.4IYY71 pKa = 11.29SPISGEE77 pKa = 3.91IIAVNAEE84 pKa = 4.41LEE86 pKa = 4.32SSPEE90 pKa = 4.01LVNSEE95 pKa = 4.23PYY97 pKa = 10.85GDD99 pKa = 4.23GFLFQIKK106 pKa = 10.29ASDD109 pKa = 3.49EE110 pKa = 4.32SEE112 pKa = 3.94IANLLSADD120 pKa = 4.26AYY122 pKa = 11.29LSGIDD127 pKa = 3.62EE128 pKa = 4.34

Molecular weight: 13.6 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A085G4Y7|A0A085G4Y7_9GAMM 30S ribosomal protein S17 OS=Ewingella americana ATCC 33852 OX=910964 GN=rpsQ PE=3 SV=1
MM1 pKa = 7.45KK2 pKa = 9.51RR3 pKa = 11.84TFQPSVLKK11 pKa = 10.6RR12 pKa = 11.84NRR14 pKa = 11.84SHH16 pKa = 7.16GFRR19 pKa = 11.84ARR21 pKa = 11.84MATKK25 pKa = 10.4NGRR28 pKa = 11.84QVLARR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 10.55GRR39 pKa = 11.84TRR41 pKa = 11.84LSASKK46 pKa = 10.74

Molecular weight: 5.37 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4395

0

4395

1403935

19

5013

319.4

35.19

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.825 ± 0.044

0.949 ± 0.013

5.205 ± 0.028

5.494 ± 0.036

3.889 ± 0.026

7.469 ± 0.041

2.198 ± 0.018

5.734 ± 0.03

4.371 ± 0.03

10.995 ± 0.05

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.677 ± 0.019

3.791 ± 0.032

4.501 ± 0.024

4.765 ± 0.033

5.29 ± 0.035

6.339 ± 0.026

5.29 ± 0.044

7.075 ± 0.032

1.38 ± 0.014

2.763 ± 0.022
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski