Petrochirus diogenes giant hermit crab associated circular virus
Average proteome isoelectric point is 8.58
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A0K1RLL5|A0A0K1RLL5_9CIRC Putative replication initiation protein OS=Petrochirus diogenes giant hermit crab associated circular virus OX=1692261 PE=4 SV=1
MM1 pKa = 6.99 EE2 pKa = 5.46 GKK4 pKa = 9.64 RR5 pKa = 11.84 VRR7 pKa = 11.84 YY8 pKa = 9.22 RR9 pKa = 11.84 RR10 pKa = 11.84 YY11 pKa = 10.17 CITINNEE18 pKa = 3.7 SDD20 pKa = 2.96 NKK22 pKa = 10.59 RR23 pKa = 11.84 ILGLFLEE30 pKa = 5.08 DD31 pKa = 4.48 GIKK34 pKa = 10.34 KK35 pKa = 10.25 GVIGVEE41 pKa = 4.11 TAPTTGTKK49 pKa = 9.96 HH50 pKa = 4.62 LQGYY54 pKa = 9.09 IEE56 pKa = 5.03 YY57 pKa = 7.41 PTQRR61 pKa = 11.84 DD62 pKa = 2.95 WSSIKK67 pKa = 10.17 KK68 pKa = 9.0 VCPNAHH74 pKa = 6.13 IEE76 pKa = 4.11 AAKK79 pKa = 8.95 GTSRR83 pKa = 11.84 QNYY86 pKa = 7.03 EE87 pKa = 3.86 YY88 pKa = 9.51 CTKK91 pKa = 10.18 EE92 pKa = 3.83 GNFVVHH98 pKa = 6.21 GHH100 pKa = 6.2 FEE102 pKa = 3.81 TGKK105 pKa = 10.04 RR106 pKa = 11.84 KK107 pKa = 9.87 RR108 pKa = 11.84 RR109 pKa = 11.84 DD110 pKa = 3.47 YY111 pKa = 11.46 SLSEE115 pKa = 3.92 YY116 pKa = 10.21 VKK118 pKa = 10.61 QVLQDD123 pKa = 3.71 EE124 pKa = 5.02 LPNDD128 pKa = 3.42 STFIRR133 pKa = 11.84 NYY135 pKa = 10.61 EE136 pKa = 4.27 KK137 pKa = 10.55 IQTHH141 pKa = 6.17 AKK143 pKa = 9.62 LVSSRR148 pKa = 11.84 QEE150 pKa = 3.65 KK151 pKa = 10.03 VRR153 pKa = 11.84 LFGEE157 pKa = 4.54 LSCCKK162 pKa = 8.76 VTSWQRR168 pKa = 11.84 KK169 pKa = 8.0 AIEE172 pKa = 4.13 SLFLQGKK179 pKa = 9.51 RR180 pKa = 11.84 EE181 pKa = 3.96 VLWIYY186 pKa = 10.62 EE187 pKa = 4.34 SVGGKK192 pKa = 10.19 GKK194 pKa = 8.24 TFLATILEE202 pKa = 4.4 VVYY205 pKa = 10.56 GFTRR209 pKa = 11.84 FDD211 pKa = 4.77 GITKK215 pKa = 10.36 SRR217 pKa = 11.84 DD218 pKa = 2.98 IALLIPPIPKK228 pKa = 10.15 GFVFDD233 pKa = 3.61 VTRR236 pKa = 11.84 DD237 pKa = 3.52 DD238 pKa = 4.83 ASNFSYY244 pKa = 9.44 NTLEE248 pKa = 4.04 QVKK251 pKa = 9.94 NGYY254 pKa = 9.36 VMSGKK259 pKa = 10.55 YY260 pKa = 10.08 GGSQHH265 pKa = 7.06 LFRR268 pKa = 11.84 PVPVIVLSNFEE279 pKa = 4.93 PIRR282 pKa = 11.84 SSLSEE287 pKa = 4.27 DD288 pKa = 2.76 RR289 pKa = 11.84 WKK291 pKa = 10.79 VWNIEE296 pKa = 3.93 NASNEE301 pKa = 4.15 KK302 pKa = 10.68 KK303 pKa = 10.33 EE304 pKa = 4.11 DD305 pKa = 3.57 LPEE308 pKa = 4.21 EE309 pKa = 4.37 GKK311 pKa = 10.25 IPPKK315 pKa = 10.4 AVLFEE320 pKa = 4.16 EE321 pKa = 4.81 EE322 pKa = 4.41 EE323 pKa = 4.24 NKK325 pKa = 10.37 EE326 pKa = 4.03 NQPNAEE332 pKa = 4.4 ASNSQQSNKK341 pKa = 10.13 DD342 pKa = 3.58 VEE344 pKa = 4.31 QHH346 pKa = 6.07 RR347 pKa = 11.84 EE348 pKa = 3.66 KK349 pKa = 11.16
Molecular weight: 40.12 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 7.925
IPC2_protein 8.053
IPC_protein 7.951
Toseland 8.331
ProMoST 8.595
Dawson 8.814
Bjellqvist 8.843
Wikipedia 8.873
Rodwell 8.931
Grimsley 8.39
Solomon 8.946
Lehninger 8.916
Nozaki 8.975
DTASelect 8.697
Thurlkill 8.799
EMBOSS 8.99
Sillero 9.019
Patrickios 4.647
IPC_peptide 8.931
IPC2_peptide 7.527
IPC2.peptide.svr19 7.628
Protein with the highest isoelectric point:
>tr|A0A0K1RLL5|A0A0K1RLL5_9CIRC Putative replication initiation protein OS=Petrochirus diogenes giant hermit crab associated circular virus OX=1692261 PE=4 SV=1
MM1 pKa = 7.42 PRR3 pKa = 11.84 MRR5 pKa = 11.84 RR6 pKa = 11.84 KK7 pKa = 8.73 KK8 pKa = 8.33 TFRR11 pKa = 11.84 KK12 pKa = 9.26 KK13 pKa = 10.75 GKK15 pKa = 6.67 FHH17 pKa = 7.05 RR18 pKa = 11.84 RR19 pKa = 11.84 RR20 pKa = 11.84 YY21 pKa = 8.13 YY22 pKa = 9.25 SKK24 pKa = 10.38 KK25 pKa = 9.59 RR26 pKa = 11.84 RR27 pKa = 11.84 TRR29 pKa = 11.84 RR30 pKa = 11.84 INLMQKK36 pKa = 9.12 QVIHH40 pKa = 6.38 NKK42 pKa = 6.64 VTRR45 pKa = 11.84 TLNNIVKK52 pKa = 9.97 SEE54 pKa = 3.98 WNTPTEE60 pKa = 3.99 LRR62 pKa = 11.84 LSIMDD67 pKa = 5.15 LLTTTEE73 pKa = 3.95 QEE75 pKa = 3.88 VFKK78 pKa = 10.97 AFKK81 pKa = 9.29 YY82 pKa = 9.59 YY83 pKa = 10.56 KK84 pKa = 9.38 IRR86 pKa = 11.84 GVKK89 pKa = 9.97 VEE91 pKa = 5.21 FKK93 pKa = 9.78 CTPPVAAIAPQAVLTSDD110 pKa = 2.84 IVHH113 pKa = 6.84 IPNVLPIMYY122 pKa = 9.59 CWEE125 pKa = 4.1 RR126 pKa = 11.84 NNDD129 pKa = 2.76 VDD131 pKa = 4.03 YY132 pKa = 10.28 TISRR136 pKa = 11.84 MKK138 pKa = 10.37 EE139 pKa = 3.61 NPNRR143 pKa = 11.84 KK144 pKa = 8.87 QFDD147 pKa = 3.87 PYY149 pKa = 10.96 KK150 pKa = 9.91 GYY152 pKa = 10.81 CKK154 pKa = 9.85 TYY156 pKa = 10.43 RR157 pKa = 11.84 GLLPEE162 pKa = 4.62 FQDD165 pKa = 3.84 KK166 pKa = 11.07 SGNNLFLNKK175 pKa = 9.97 KK176 pKa = 8.74 SWISSADD183 pKa = 3.16 TTYY186 pKa = 11.21 LYY188 pKa = 11.27 GRR190 pKa = 11.84 FIFMPNIISTDD201 pKa = 3.44 ALIVNYY207 pKa = 10.42 NFDD210 pKa = 3.77 IDD212 pKa = 3.78 LTIYY216 pKa = 10.84 YY217 pKa = 9.81 SLKK220 pKa = 8.54 TLQYY224 pKa = 10.05 TII226 pKa = 4.6
Molecular weight: 27.04 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.227
IPC2_protein 9.619
IPC_protein 9.736
Toseland 10.175
ProMoST 9.867
Dawson 10.379
Bjellqvist 10.043
Wikipedia 10.54
Rodwell 10.818
Grimsley 10.452
Solomon 10.394
Lehninger 10.35
Nozaki 10.16
DTASelect 10.043
Thurlkill 10.218
EMBOSS 10.57
Sillero 10.277
Patrickios 10.409
IPC_peptide 10.394
IPC2_peptide 8.756
IPC2.peptide.svr19 8.574
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2
0
2
575
226
349
287.5
33.58
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
3.478 ± 0.211
1.391 ± 0.035
4.174 ± 0.139
7.304 ± 2.081
4.522 ± 0.191
5.217 ± 1.417
1.739 ± 0.228
6.783 ± 0.653
9.565 ± 0.338
6.957 ± 0.313
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.565 ± 0.847
6.261 ± 0.453
4.348 ± 0.287
3.826 ± 0.403
7.13 ± 0.706
6.435 ± 0.867
6.435 ± 1.09
6.435 ± 0.622
1.391 ± 0.035
5.043 ± 0.881
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here