Petrochirus diogenes giant hermit crab associated circular virus 
Average proteome isoelectric point is 8.58 
Get precalculated fractions of proteins 
 
  
    Acidic  
 
  
    pI < 6.8  
 
  
    6.8-7.4  
 
  
    pI > 7.4  
 
  
    Basic  
    
 
  
    All  
 
Note: above files contain also dissociation constants (pKa) 
Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein) 
 
Get csv file with sequences according to given criteria: 
* You can choose from 21 different methods for calculating isoelectric point 
 Summary statistics related to proteome-wise predictions 
Protein with the lowest isoelectric point: 
>tr|A0A0K1RLL5|A0A0K1RLL5_9CIRC Putative replication initiation protein OS=Petrochirus diogenes giant hermit crab associated circular virus OX=1692261 PE=4 SV=1MM1 pKa = 6.99  EE2 pKa = 5.46  GKK4 pKa = 9.64  RR5 pKa = 11.84  VRR7 pKa = 11.84  YY8 pKa = 9.22  RR9 pKa = 11.84  RR10 pKa = 11.84  YY11 pKa = 10.17  CITINNEE18 pKa = 3.7  SDD20 pKa = 2.96  NKK22 pKa = 10.59  RR23 pKa = 11.84  ILGLFLEE30 pKa = 5.08  DD31 pKa = 4.48  GIKK34 pKa = 10.34  KK35 pKa = 10.25  GVIGVEE41 pKa = 4.11  TAPTTGTKK49 pKa = 9.96  HH50 pKa = 4.62  LQGYY54 pKa = 9.09  IEE56 pKa = 5.03  YY57 pKa = 7.41  PTQRR61 pKa = 11.84  DD62 pKa = 2.95  WSSIKK67 pKa = 10.17  KK68 pKa = 9.0  VCPNAHH74 pKa = 6.13  IEE76 pKa = 4.11  AAKK79 pKa = 8.95  GTSRR83 pKa = 11.84  QNYY86 pKa = 7.03  EE87 pKa = 3.86  YY88 pKa = 9.51  CTKK91 pKa = 10.18  EE92 pKa = 3.83  GNFVVHH98 pKa = 6.21  GHH100 pKa = 6.2  FEE102 pKa = 3.81  TGKK105 pKa = 10.04  RR106 pKa = 11.84  KK107 pKa = 9.87  RR108 pKa = 11.84  RR109 pKa = 11.84  DD110 pKa = 3.47  YY111 pKa = 11.46  SLSEE115 pKa = 3.92  YY116 pKa = 10.21  VKK118 pKa = 10.61  QVLQDD123 pKa = 3.71  EE124 pKa = 5.02  LPNDD128 pKa = 3.42  STFIRR133 pKa = 11.84  NYY135 pKa = 10.61  EE136 pKa = 4.27  KK137 pKa = 10.55  IQTHH141 pKa = 6.17  AKK143 pKa = 9.62  LVSSRR148 pKa = 11.84  QEE150 pKa = 3.65  KK151 pKa = 10.03  VRR153 pKa = 11.84  LFGEE157 pKa = 4.54  LSCCKK162 pKa = 8.76  VTSWQRR168 pKa = 11.84  KK169 pKa = 8.0  AIEE172 pKa = 4.13  SLFLQGKK179 pKa = 9.51  RR180 pKa = 11.84  EE181 pKa = 3.96  VLWIYY186 pKa = 10.62  EE187 pKa = 4.34  SVGGKK192 pKa = 10.19  GKK194 pKa = 8.24  TFLATILEE202 pKa = 4.4  VVYY205 pKa = 10.56  GFTRR209 pKa = 11.84  FDD211 pKa = 4.77  GITKK215 pKa = 10.36  SRR217 pKa = 11.84  DD218 pKa = 2.98  IALLIPPIPKK228 pKa = 10.15  GFVFDD233 pKa = 3.61  VTRR236 pKa = 11.84  DD237 pKa = 3.52  DD238 pKa = 4.83  ASNFSYY244 pKa = 9.44  NTLEE248 pKa = 4.04  QVKK251 pKa = 9.94  NGYY254 pKa = 9.36  VMSGKK259 pKa = 10.55  YY260 pKa = 10.08  GGSQHH265 pKa = 7.06  LFRR268 pKa = 11.84  PVPVIVLSNFEE279 pKa = 4.93  PIRR282 pKa = 11.84  SSLSEE287 pKa = 4.27  DD288 pKa = 2.76  RR289 pKa = 11.84  WKK291 pKa = 10.79  VWNIEE296 pKa = 3.93  NASNEE301 pKa = 4.15  KK302 pKa = 10.68  KK303 pKa = 10.33  EE304 pKa = 4.11  DD305 pKa = 3.57  LPEE308 pKa = 4.21  EE309 pKa = 4.37  GKK311 pKa = 10.25  IPPKK315 pKa = 10.4  AVLFEE320 pKa = 4.16  EE321 pKa = 4.81  EE322 pKa = 4.41  EE323 pKa = 4.24  NKK325 pKa = 10.37  EE326 pKa = 4.03  NQPNAEE332 pKa = 4.4  ASNSQQSNKK341 pKa = 10.13  DD342 pKa = 3.58  VEE344 pKa = 4.31  QHH346 pKa = 6.07  RR347 pKa = 11.84  EE348 pKa = 3.66  KK349 pKa = 11.16  
 40.12 kDa
Isoelectric point according different methods: 
IPC2.protein.svr19  7.925 
IPC2_protein 8.053 
IPC_protein 7.951 
Toseland    8.331 
ProMoST     8.595 
Dawson      8.814 
Bjellqvist  8.843 
Wikipedia   8.873 
Rodwell     8.931 
Grimsley    8.39 
Solomon     8.946 
Lehninger   8.916 
Nozaki      8.975 
DTASelect   8.697 
Thurlkill   8.799 
EMBOSS      8.99 
Sillero     9.019 
Patrickios  4.647 
IPC_peptide 8.931 
IPC2_peptide  7.527 
IPC2.peptide.svr19  7.628 
 Protein with the highest isoelectric point: 
>tr|A0A0K1RLL5|A0A0K1RLL5_9CIRC Putative replication initiation protein OS=Petrochirus diogenes giant hermit crab associated circular virus OX=1692261 PE=4 SV=1MM1 pKa = 7.42  PRR3 pKa = 11.84  MRR5 pKa = 11.84  RR6 pKa = 11.84  KK7 pKa = 8.73  KK8 pKa = 8.33  TFRR11 pKa = 11.84  KK12 pKa = 9.26  KK13 pKa = 10.75  GKK15 pKa = 6.67  FHH17 pKa = 7.05  RR18 pKa = 11.84  RR19 pKa = 11.84  RR20 pKa = 11.84  YY21 pKa = 8.13  YY22 pKa = 9.25  SKK24 pKa = 10.38  KK25 pKa = 9.59  RR26 pKa = 11.84  RR27 pKa = 11.84  TRR29 pKa = 11.84  RR30 pKa = 11.84  INLMQKK36 pKa = 9.12  QVIHH40 pKa = 6.38  NKK42 pKa = 6.64  VTRR45 pKa = 11.84  TLNNIVKK52 pKa = 9.97  SEE54 pKa = 3.98  WNTPTEE60 pKa = 3.99  LRR62 pKa = 11.84  LSIMDD67 pKa = 5.15  LLTTTEE73 pKa = 3.95  QEE75 pKa = 3.88  VFKK78 pKa = 10.97  AFKK81 pKa = 9.29  YY82 pKa = 9.59  YY83 pKa = 10.56  KK84 pKa = 9.38  IRR86 pKa = 11.84  GVKK89 pKa = 9.97  VEE91 pKa = 5.21  FKK93 pKa = 9.78  CTPPVAAIAPQAVLTSDD110 pKa = 2.84  IVHH113 pKa = 6.84  IPNVLPIMYY122 pKa = 9.59  CWEE125 pKa = 4.1  RR126 pKa = 11.84  NNDD129 pKa = 2.76  VDD131 pKa = 4.03  YY132 pKa = 10.28  TISRR136 pKa = 11.84  MKK138 pKa = 10.37  EE139 pKa = 3.61  NPNRR143 pKa = 11.84  KK144 pKa = 8.87  QFDD147 pKa = 3.87  PYY149 pKa = 10.96  KK150 pKa = 9.91  GYY152 pKa = 10.81  CKK154 pKa = 9.85  TYY156 pKa = 10.43  RR157 pKa = 11.84  GLLPEE162 pKa = 4.62  FQDD165 pKa = 3.84  KK166 pKa = 11.07  SGNNLFLNKK175 pKa = 9.97  KK176 pKa = 8.74  SWISSADD183 pKa = 3.16  TTYY186 pKa = 11.21  LYY188 pKa = 11.27  GRR190 pKa = 11.84  FIFMPNIISTDD201 pKa = 3.44  ALIVNYY207 pKa = 10.42  NFDD210 pKa = 3.77  IDD212 pKa = 3.78  LTIYY216 pKa = 10.84  YY217 pKa = 9.81  SLKK220 pKa = 8.54  TLQYY224 pKa = 10.05  TII226 pKa = 4.6  
 27.04 kDa
Isoelectric point according different methods: 
IPC2.protein.svr19  9.227 
IPC2_protein 9.619 
IPC_protein 9.736 
Toseland    10.175 
ProMoST     9.867 
Dawson      10.379 
Bjellqvist  10.043 
Wikipedia   10.54 
Rodwell     10.818 
Grimsley    10.452 
Solomon     10.394 
Lehninger   10.35 
Nozaki      10.16 
DTASelect   10.043 
Thurlkill   10.218 
EMBOSS      10.57 
Sillero     10.277 
Patrickios  10.409 
IPC_peptide 10.394 
IPC2_peptide  8.756 
IPC2.peptide.svr19  8.574 
 Peptides (in silico  digests for buttom-up proteomics) 
Below you can find 
in silico  digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
 
  
    Try  
 
  
    ChTry  
 
  
    ArgC  
 
  
    LysN  
 
  
    TryLysC  
 
  
    Try  
 
  
    ChTry  
 
  
    ArgC  
 
  
    LysN  
 
  
    TryLysC  
 
  
    Try  
 
  
    ChTry  
 
  
    ArgC  
 
  
    LysN  
 
  
    TryLysC  
 
  
    Try  
 
  
    ChTry  
 
  
    ArgC  
 
  
    LysN  
 
  
    TryLysC  
 
  
    Try  
 
  
    ChTry  
 
  
    ArgC  
 
  
    LysN  
 
  
    TryLysC  
   
  
General Statistics 
    
      
        Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
 
     
    
      
        2 
0
2 
575
226
349
287.5
33.58
          
     
  
 
    Amino acid frequency 
  
  
    
      
        Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
 
     
    
                 
        3.478 ± 0.211
1.391 ± 0.035
4.174 ± 0.139
7.304 ± 2.081
4.522 ± 0.191
5.217 ± 1.417
1.739 ± 0.228
6.783 ± 0.653
9.565 ± 0.338
6.957 ± 0.313
          
     
  
  
  
      
          
        Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
 
     
    
                 
        1.565 ± 0.847
6.261 ± 0.453
4.348 ± 0.287
3.826 ± 0.403
7.13 ± 0.706
6.435 ± 0.867
6.435 ± 1.09
6.435 ± 0.622
1.391 ± 0.035
5.043 ± 0.881
          
     
  
  
  
Note:  For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein levelMost of the basic statistics you can see at this page can be downloaded from this CSV file  
For dipeptide frequency statistics click here