Acanthamoeba polyphaga moumouvirus

Taxonomy: Viruses; Varidnaviria; Bamfordvirae; Nucleocytoviricota; Megaviricetes; Imitervirales; Mimiviridae; unclassified Mimiviridae; Moumouvirus

Average proteome isoelectric point is 6.75

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 894 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|L7RCT0|L7RCT0_9VIRU Thymidylate synthase OS=Acanthamoeba polyphaga moumouvirus OX=1269028 GN=Moumou_00308 PE=3 SV=1
MM1 pKa = 7.45SKK3 pKa = 10.81YY4 pKa = 9.56GTINFTYY11 pKa = 9.94GGPYY15 pKa = 10.0NDD17 pKa = 4.82IIGSASYY24 pKa = 11.28YY25 pKa = 10.8DD26 pKa = 4.57DD27 pKa = 3.25GTVNIKK33 pKa = 8.87YY34 pKa = 8.43TLLSLKK40 pKa = 10.4NLLLHH45 pKa = 6.24NVNIIDD51 pKa = 5.41KK52 pKa = 10.59LLEE55 pKa = 4.18NNNIKK60 pKa = 10.78NIMVNDD66 pKa = 3.65TDD68 pKa = 3.52ISVEE72 pKa = 3.79VDD74 pKa = 3.29SNEE77 pKa = 4.5SYY79 pKa = 10.6EE80 pKa = 4.15QLVKK84 pKa = 11.2DD85 pKa = 3.48EE86 pKa = 4.2VLINEE91 pKa = 4.31TDD93 pKa = 3.29NFSDD97 pKa = 3.76YY98 pKa = 11.16SGEE101 pKa = 3.95IEE103 pKa = 4.34TNQDD107 pKa = 2.65RR108 pKa = 11.84LNRR111 pKa = 11.84VLNMTNINEE120 pKa = 4.28TEE122 pKa = 4.06NIFGNEE128 pKa = 3.92IKK130 pKa = 10.58SDD132 pKa = 4.23DD133 pKa = 3.86IFNSDD138 pKa = 3.97SEE140 pKa = 4.51NSDD143 pKa = 3.59SEE145 pKa = 5.34SNDD148 pKa = 3.42LLNDD152 pKa = 4.05DD153 pKa = 4.93KK154 pKa = 11.74AHH156 pKa = 7.04KK157 pKa = 10.32YY158 pKa = 10.17LFNAFEE164 pKa = 4.28LLINSEE170 pKa = 4.21KK171 pKa = 10.62DD172 pKa = 2.93IHH174 pKa = 7.02SDD176 pKa = 2.99SDD178 pKa = 3.36

Molecular weight: 20.29 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|L7RC44|L7RC44_9VIRU Uncharacterized protein OS=Acanthamoeba polyphaga moumouvirus OX=1269028 GN=Moumou_00297 PE=4 SV=1
MM1 pKa = 7.23KK2 pKa = 10.27QKK4 pKa = 10.07QQFKK8 pKa = 10.43QKK10 pKa = 9.64QQLRR14 pKa = 11.84QASKK18 pKa = 11.29KK19 pKa = 9.42NVEE22 pKa = 4.16PNFTVVNNKK31 pKa = 10.32AIDD34 pKa = 3.95LLVEE38 pKa = 3.95EE39 pKa = 5.08LVSIRR44 pKa = 11.84CASDD48 pKa = 3.11VTQRR52 pKa = 11.84GPARR56 pKa = 11.84HH57 pKa = 6.25CAAIFIEE64 pKa = 4.41KK65 pKa = 9.85NQSCFKK71 pKa = 9.85PTINWYY77 pKa = 7.99QSRR80 pKa = 11.84EE81 pKa = 4.08LLPEE85 pKa = 3.96EE86 pKa = 3.7ARR88 pKa = 11.84KK89 pKa = 9.69RR90 pKa = 11.84SRR92 pKa = 11.84KK93 pKa = 6.93KK94 pKa = 8.81QRR96 pKa = 11.84RR97 pKa = 11.84RR98 pKa = 11.84RR99 pKa = 11.84YY100 pKa = 9.61RR101 pKa = 11.84SISVV105 pKa = 3.03

Molecular weight: 12.49 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

894

0

894

290542

43

2914

325.0

37.73

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

2.975 ± 0.074

1.835 ± 0.053

6.319 ± 0.073

5.727 ± 0.077

4.734 ± 0.064

4.53 ± 0.174

1.991 ± 0.039

10.514 ± 0.118

9.252 ± 0.142

8.526 ± 0.084

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.049 ± 0.043

9.926 ± 0.144

3.255 ± 0.063

3.004 ± 0.056

3.035 ± 0.069

6.428 ± 0.072

4.854 ± 0.073

4.396 ± 0.068

0.819 ± 0.027

5.833 ± 0.076
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski