Faba bean necrotic stunt alphasatellite

Taxonomy: Viruses; Alphasatellitidae; Nanoalphasatellitinae; Mivedwarsatellite

Average proteome isoelectric point is 6.7

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 1 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|V9TSQ1|V9TSQ1_9VIRU Satellite replication initiator protein 2 OS=Faba bean necrotic stunt alphasatellite OX=1441773 PE=4 SV=1
MM1 pKa = 7.38QSVSWVFTLNFSGEE15 pKa = 4.21VPLLSFDD22 pKa = 3.79EE23 pKa = 4.35RR24 pKa = 11.84VQYY27 pKa = 10.7AVWQHH32 pKa = 4.84EE33 pKa = 4.66RR34 pKa = 11.84VNHH37 pKa = 6.17DD38 pKa = 3.43HH39 pKa = 6.34LQGVIQMKK47 pKa = 9.87RR48 pKa = 11.84KK49 pKa = 7.79TRR51 pKa = 11.84MNTVKK56 pKa = 10.57SLIGGNPHH64 pKa = 7.06LEE66 pKa = 4.11PMRR69 pKa = 11.84SRR71 pKa = 11.84IEE73 pKa = 3.86EE74 pKa = 3.92ASAYY78 pKa = 10.16AMKK81 pKa = 10.57EE82 pKa = 3.97EE83 pKa = 4.32SRR85 pKa = 11.84VAGPWFHH92 pKa = 7.46GEE94 pKa = 3.99LLKK97 pKa = 11.05KK98 pKa = 10.69GSNKK102 pKa = 9.34RR103 pKa = 11.84SLMEE107 pKa = 4.5KK108 pKa = 10.04YY109 pKa = 10.15KK110 pKa = 10.41EE111 pKa = 4.44DD112 pKa = 3.8PVSMEE117 pKa = 4.63LEE119 pKa = 4.52DD120 pKa = 3.86PGKK123 pKa = 10.53ARR125 pKa = 11.84RR126 pKa = 11.84CRR128 pKa = 11.84ARR130 pKa = 11.84LDD132 pKa = 3.39KK133 pKa = 10.82EE134 pKa = 4.02KK135 pKa = 10.55FVAAFKK141 pKa = 11.3VEE143 pKa = 4.55DD144 pKa = 5.56DD145 pKa = 3.65EE146 pKa = 5.61QEE148 pKa = 3.86WKK150 pKa = 10.6RR151 pKa = 11.84LLEE154 pKa = 4.27EE155 pKa = 4.17EE156 pKa = 4.54LEE158 pKa = 4.41KK159 pKa = 11.23VPSPRR164 pKa = 11.84SILWVYY170 pKa = 10.6GPTGGEE176 pKa = 4.16GKK178 pKa = 6.22TTKK181 pKa = 10.45AKK183 pKa = 10.55EE184 pKa = 4.01LITRR188 pKa = 11.84GWFYY192 pKa = 11.06TRR194 pKa = 11.84GGRR197 pKa = 11.84KK198 pKa = 9.47DD199 pKa = 3.67DD200 pKa = 3.42VAYY203 pKa = 10.73SYY205 pKa = 11.22IEE207 pKa = 4.41DD208 pKa = 3.8PTRR211 pKa = 11.84HH212 pKa = 5.0VVFDD216 pKa = 3.91IPRR219 pKa = 11.84DD220 pKa = 3.59MKK222 pKa = 10.99DD223 pKa = 3.1YY224 pKa = 11.11CNYY227 pKa = 11.01SLIEE231 pKa = 3.98MLKK234 pKa = 10.7DD235 pKa = 4.34RR236 pKa = 11.84IIISNKK242 pKa = 8.9YY243 pKa = 9.9EE244 pKa = 4.05PVTNCQLSNIHH255 pKa = 6.33VIVMANFLPDD265 pKa = 3.29YY266 pKa = 10.6CKK268 pKa = 10.41ISEE271 pKa = 4.01DD272 pKa = 4.08RR273 pKa = 11.84INIIYY278 pKa = 10.08CC279 pKa = 3.79

Molecular weight: 32.63 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|V9TSQ1|V9TSQ1_9VIRU Satellite replication initiator protein 2 OS=Faba bean necrotic stunt alphasatellite OX=1441773 PE=4 SV=1
MM1 pKa = 7.38QSVSWVFTLNFSGEE15 pKa = 4.21VPLLSFDD22 pKa = 3.79EE23 pKa = 4.35RR24 pKa = 11.84VQYY27 pKa = 10.7AVWQHH32 pKa = 4.84EE33 pKa = 4.66RR34 pKa = 11.84VNHH37 pKa = 6.17DD38 pKa = 3.43HH39 pKa = 6.34LQGVIQMKK47 pKa = 9.87RR48 pKa = 11.84KK49 pKa = 7.79TRR51 pKa = 11.84MNTVKK56 pKa = 10.57SLIGGNPHH64 pKa = 7.06LEE66 pKa = 4.11PMRR69 pKa = 11.84SRR71 pKa = 11.84IEE73 pKa = 3.86EE74 pKa = 3.92ASAYY78 pKa = 10.16AMKK81 pKa = 10.57EE82 pKa = 3.97EE83 pKa = 4.32SRR85 pKa = 11.84VAGPWFHH92 pKa = 7.46GEE94 pKa = 3.99LLKK97 pKa = 11.05KK98 pKa = 10.69GSNKK102 pKa = 9.34RR103 pKa = 11.84SLMEE107 pKa = 4.5KK108 pKa = 10.04YY109 pKa = 10.15KK110 pKa = 10.41EE111 pKa = 4.44DD112 pKa = 3.8PVSMEE117 pKa = 4.63LEE119 pKa = 4.52DD120 pKa = 3.86PGKK123 pKa = 10.53ARR125 pKa = 11.84RR126 pKa = 11.84CRR128 pKa = 11.84ARR130 pKa = 11.84LDD132 pKa = 3.39KK133 pKa = 10.82EE134 pKa = 4.02KK135 pKa = 10.55FVAAFKK141 pKa = 11.3VEE143 pKa = 4.55DD144 pKa = 5.56DD145 pKa = 3.65EE146 pKa = 5.61QEE148 pKa = 3.86WKK150 pKa = 10.6RR151 pKa = 11.84LLEE154 pKa = 4.27EE155 pKa = 4.17EE156 pKa = 4.54LEE158 pKa = 4.41KK159 pKa = 11.23VPSPRR164 pKa = 11.84SILWVYY170 pKa = 10.6GPTGGEE176 pKa = 4.16GKK178 pKa = 6.22TTKK181 pKa = 10.45AKK183 pKa = 10.55EE184 pKa = 4.01LITRR188 pKa = 11.84GWFYY192 pKa = 11.06TRR194 pKa = 11.84GGRR197 pKa = 11.84KK198 pKa = 9.47DD199 pKa = 3.67DD200 pKa = 3.42VAYY203 pKa = 10.73SYY205 pKa = 11.22IEE207 pKa = 4.41DD208 pKa = 3.8PTRR211 pKa = 11.84HH212 pKa = 5.0VVFDD216 pKa = 3.91IPRR219 pKa = 11.84DD220 pKa = 3.59MKK222 pKa = 10.99DD223 pKa = 3.1YY224 pKa = 11.11CNYY227 pKa = 11.01SLIEE231 pKa = 3.98MLKK234 pKa = 10.7DD235 pKa = 4.34RR236 pKa = 11.84IIISNKK242 pKa = 8.9YY243 pKa = 9.9EE244 pKa = 4.05PVTNCQLSNIHH255 pKa = 6.33VIVMANFLPDD265 pKa = 3.29YY266 pKa = 10.6CKK268 pKa = 10.41ISEE271 pKa = 4.01DD272 pKa = 4.08RR273 pKa = 11.84INIIYY278 pKa = 10.08CC279 pKa = 3.79

Molecular weight: 32.63 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

1

0

1

279

279

279

279.0

32.63

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

4.301 ± 0.0

1.792 ± 0.0

5.735 ± 0.0

9.677 ± 0.0

3.226 ± 0.0

5.376 ± 0.0

2.509 ± 0.0

6.093 ± 0.0

8.244 ± 0.0

7.168 ± 0.0

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.584 ± 0.0

3.943 ± 0.0

4.659 ± 0.0

2.509 ± 0.0

7.527 ± 0.0

6.452 ± 0.0

3.584 ± 0.0

7.168 ± 0.0

2.151 ± 0.0

4.301 ± 0.0
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski