Mastacembelus armatus (zig-zag eel)

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Metazoa; Eumetazoa; Bilateria; Deuterostomia; Chordata; Craniata; Vertebrata; Gnathostomata; Teleostomi; Euteleostomi; Actinopterygii; Actinopteri; Neopterygii; Teleostei; Osteoglossocephalai; Clupeocephala; Euteleosteomorpha; Neoteleostei; Eurypterygia;

Average proteome isoelectric point is 6.46

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 59116 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A7N8YH35|A0A7N8YH35_9TELE Isoform of A0A7N8YM48 Protein unc-13 homolog B-like OS=Mastacembelus armatus OX=205130 PE=4 SV=1
MM1 pKa = 7.42SLLSGMGGMQPRR13 pKa = 11.84PSCVPNPCHH22 pKa = 6.72PGVKK26 pKa = 9.69CVVTPEE32 pKa = 4.07GTKK35 pKa = 10.48CGPCPDD41 pKa = 3.56GTEE44 pKa = 4.51GNGTHH49 pKa = 6.04CTDD52 pKa = 2.87VDD54 pKa = 4.23EE55 pKa = 4.65VCRR58 pKa = 11.84DD59 pKa = 3.15INEE62 pKa = 4.46CEE64 pKa = 4.11GPNNGGCVEE73 pKa = 4.76NSVCMNTPGSFRR85 pKa = 11.84CGPCNPGYY93 pKa = 11.0VGDD96 pKa = 4.05QRR98 pKa = 11.84QGCKK102 pKa = 9.8PEE104 pKa = 3.97RR105 pKa = 11.84ACGNGQPNPCHH116 pKa = 6.71ASAEE120 pKa = 4.62CIVHH124 pKa = 6.66RR125 pKa = 11.84EE126 pKa = 4.01GTIEE130 pKa = 4.18CQCGVGWAGNGFLCGSDD147 pKa = 2.85IDD149 pKa = 4.15IDD151 pKa = 3.95GFPDD155 pKa = 3.4EE156 pKa = 5.89KK157 pKa = 10.96LDD159 pKa = 4.25CPEE162 pKa = 4.42RR163 pKa = 11.84NCDD166 pKa = 3.95KK167 pKa = 10.9DD168 pKa = 3.45NCVNVPNSGQEE179 pKa = 3.78DD180 pKa = 4.02ADD182 pKa = 4.12RR183 pKa = 11.84DD184 pKa = 4.5GIGDD188 pKa = 4.0ACDD191 pKa = 3.53EE192 pKa = 4.74DD193 pKa = 5.71ADD195 pKa = 4.76GDD197 pKa = 4.64GIPNTQDD204 pKa = 2.78NCVLVPNLDD213 pKa = 3.49QRR215 pKa = 11.84NVDD218 pKa = 3.55EE219 pKa = 6.13DD220 pKa = 4.52DD221 pKa = 5.62FGDD224 pKa = 3.87ACDD227 pKa = 3.29NCRR230 pKa = 11.84AVKK233 pKa = 10.88NNDD236 pKa = 3.36QKK238 pKa = 11.3DD239 pKa = 3.35TDD241 pKa = 3.93LDD243 pKa = 3.96KK244 pKa = 11.55FGDD247 pKa = 3.9EE248 pKa = 4.55CDD250 pKa = 3.68EE251 pKa = 5.81DD252 pKa = 4.18IDD254 pKa = 5.21GDD256 pKa = 4.45GIPNHH261 pKa = 7.08LDD263 pKa = 2.97NCKK266 pKa = 9.84RR267 pKa = 11.84VPNADD272 pKa = 3.2QKK274 pKa = 11.71DD275 pKa = 3.63RR276 pKa = 11.84DD277 pKa = 3.66GDD279 pKa = 4.12KK280 pKa = 11.46VGDD283 pKa = 4.05ACDD286 pKa = 3.38SCPYY290 pKa = 10.12VPNPDD295 pKa = 3.95QMDD298 pKa = 3.39VDD300 pKa = 4.01NDD302 pKa = 4.86LIGDD306 pKa = 3.96PCDD309 pKa = 3.62TNKK312 pKa = 10.93DD313 pKa = 3.49SDD315 pKa = 4.16GDD317 pKa = 3.81GHH319 pKa = 6.91QDD321 pKa = 4.2SRR323 pKa = 11.84DD324 pKa = 3.47NCPAVINSSQLDD336 pKa = 3.5TDD338 pKa = 3.78KK339 pKa = 11.37DD340 pKa = 4.07GKK342 pKa = 11.12GDD344 pKa = 3.71EE345 pKa = 5.3CDD347 pKa = 5.61DD348 pKa = 5.63DD349 pKa = 6.35DD350 pKa = 7.03DD351 pKa = 6.2NDD353 pKa = 5.44GIPDD357 pKa = 4.89LLPPGPDD364 pKa = 3.07NCRR367 pKa = 11.84LIYY370 pKa = 10.78NPLQEE375 pKa = 5.74DD376 pKa = 3.52SDD378 pKa = 4.45GDD380 pKa = 4.06GVGNVCEE387 pKa = 4.73KK388 pKa = 11.08DD389 pKa = 3.31FDD391 pKa = 4.21NDD393 pKa = 3.98TVIDD397 pKa = 4.44TIDD400 pKa = 3.4VCPEE404 pKa = 3.65NAEE407 pKa = 4.04VTLTDD412 pKa = 3.59FRR414 pKa = 11.84EE415 pKa = 4.27YY416 pKa = 9.18QTVVLDD422 pKa = 4.15PEE424 pKa = 5.32GDD426 pKa = 3.65AQIDD430 pKa = 4.13PNWVVLNQGRR440 pKa = 11.84EE441 pKa = 3.9IVQTMNSDD449 pKa = 3.19PGLAVGYY456 pKa = 7.12TAFSGVDD463 pKa = 3.72FEE465 pKa = 5.24GTFHH469 pKa = 6.81VNTVTDD475 pKa = 3.57DD476 pKa = 4.08DD477 pKa = 4.35YY478 pKa = 12.0AGFIFGYY485 pKa = 9.58QDD487 pKa = 2.83SSSFYY492 pKa = 9.91VVMWKK497 pKa = 9.52QVEE500 pKa = 4.18QIYY503 pKa = 8.33WQANPFRR510 pKa = 11.84AVAEE514 pKa = 4.08PGIQLKK520 pKa = 10.06AVKK523 pKa = 10.35SNTGPGEE530 pKa = 4.06NLRR533 pKa = 11.84NALWHH538 pKa = 6.46TGDD541 pKa = 3.25TTDD544 pKa = 4.87QVKK547 pKa = 10.41LLWKK551 pKa = 10.3DD552 pKa = 3.26SRR554 pKa = 11.84NVGWKK559 pKa = 10.42DD560 pKa = 2.97KK561 pKa = 10.83TSYY564 pKa = 10.52RR565 pKa = 11.84WFLQHH570 pKa = 6.87RR571 pKa = 11.84PADD574 pKa = 3.73GYY576 pKa = 10.45IRR578 pKa = 11.84VRR580 pKa = 11.84FYY582 pKa = 11.03EE583 pKa = 4.61GPKK586 pKa = 9.26MVADD590 pKa = 3.62TGVIIDD596 pKa = 3.35ATMRR600 pKa = 11.84GGRR603 pKa = 11.84LGVFCFSQEE612 pKa = 3.95NIIWANLRR620 pKa = 11.84YY621 pKa = 9.71RR622 pKa = 11.84CNDD625 pKa = 3.48TLPEE629 pKa = 4.41DD630 pKa = 3.9FDD632 pKa = 3.96TYY634 pKa = 10.72RR635 pKa = 11.84AQQVQLVAA643 pKa = 4.4

Molecular weight: 70.3 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A3Q3NFI3|A0A3Q3NFI3_9TELE Uncharacterized protein OS=Mastacembelus armatus OX=205130 PE=4 SV=1
SS1 pKa = 4.63SHH3 pKa = 5.15KK4 pKa = 8.9TFRR7 pKa = 11.84IKK9 pKa = 10.64RR10 pKa = 11.84FLAKK14 pKa = 9.71KK15 pKa = 9.58QKK17 pKa = 8.69QNRR20 pKa = 11.84PIPQWIRR27 pKa = 11.84MKK29 pKa = 9.89TGNKK33 pKa = 8.61IRR35 pKa = 11.84YY36 pKa = 7.09NSKK39 pKa = 8.3RR40 pKa = 11.84RR41 pKa = 11.84HH42 pKa = 3.95WRR44 pKa = 11.84RR45 pKa = 11.84TKK47 pKa = 10.83LGLL50 pKa = 3.67

Molecular weight: 6.28 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

22295

36821

59116

40357664

17

8413

682.7

76.49

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.359 ± 0.008

2.313 ± 0.008

5.24 ± 0.006

6.721 ± 0.01

3.819 ± 0.007

6.167 ± 0.008

2.669 ± 0.004

4.754 ± 0.007

5.686 ± 0.009

9.775 ± 0.011

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.428 ± 0.004

3.968 ± 0.006

5.376 ± 0.011

4.726 ± 0.008

5.446 ± 0.007

8.266 ± 0.011

5.669 ± 0.007

6.482 ± 0.008

1.194 ± 0.003

2.94 ± 0.005
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski