Amino acid dipepetide frequency for Synechococcus sp. CB0101

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	16.736AlaAla: 16.736 ± 0.212	1.523AlaCys: 1.523 ± 0.047	6.11AlaAsp: 6.11 ± 0.09	7.417AlaGlu: 7.417 ± 0.117	3.278AlaPhe: 3.278 ± 0.063	9.841AlaGly: 9.841 ± 0.128	1.881AlaHis: 1.881 ± 0.051	5.011AlaIle: 5.011 ± 0.086	2.61AlaLys: 2.61 ± 0.078	15.68AlaLeu: 15.68 ± 0.205	2.932AlaMet: 2.932 ± 0.06	2.594AlaAsn: 2.594 ± 0.06	5.858AlaPro: 5.858 ± 0.113	4.955AlaGln: 4.955 ± 0.089	6.704AlaArg: 6.704 ± 0.106	6.843AlaSer: 6.843 ± 0.092	4.809AlaThr: 4.809 ± 0.083	8.659AlaVal: 8.659 ± 0.115	1.954AlaTrp: 1.954 ± 0.049	2.064AlaTyr: 2.064 ± 0.051	0.0AlaXaa: 0.0 ± 0.0
Cys	0.972CysAla: 0.972 ± 0.041	0.271CysCys: 0.271 ± 0.019	0.635CysAsp: 0.635 ± 0.026	0.61CysGlu: 0.61 ± 0.028	0.435CysPhe: 0.435 ± 0.024	1.282CysGly: 1.282 ± 0.043	0.301CysHis: 0.301 ± 0.021	0.437CysIle: 0.437 ± 0.024	0.248CysLys: 0.248 ± 0.016	1.376CysLeu: 1.376 ± 0.039	0.182CysMet: 0.182 ± 0.015	0.323CysAsn: 0.323 ± 0.021	0.678CysPro: 0.678 ± 0.033	0.511CysGln: 0.511 ± 0.025	1.041CysArg: 1.041 ± 0.037	0.918CysSer: 0.918 ± 0.031	0.591CysThr: 0.591 ± 0.029	0.751CysVal: 0.751 ± 0.033	0.276CysTrp: 0.276 ± 0.018	0.206CysTyr: 0.206 ± 0.016	0.002CysXaa: 0.002 ± 0.002
Asp	5.302AspAla: 5.302 ± 0.076	0.514AspCys: 0.514 ± 0.026	2.226AspAsp: 2.226 ± 0.065	2.446AspGlu: 2.446 ± 0.06	1.46AspPhe: 1.46 ± 0.044	4.58AspGly: 4.58 ± 0.083	1.17AspHis: 1.17 ± 0.043	1.367AspIle: 1.367 ± 0.047	0.957AspLys: 0.957 ± 0.039	6.752AspLeu: 6.752 ± 0.098	0.644AspMet: 0.644 ± 0.034	1.024AspAsn: 1.024 ± 0.037	3.971AspPro: 3.971 ± 0.069	2.85AspGln: 2.85 ± 0.062	3.547AspArg: 3.547 ± 0.066	2.184AspSer: 2.184 ± 0.056	1.914AspThr: 1.914 ± 0.06	2.95AspVal: 2.95 ± 0.061	0.987AspTrp: 0.987 ± 0.033	1.076AspTyr: 1.076 ± 0.038	0.0AspXaa: 0.0 ± 0.0
Glu	8.279GluAla: 8.279 ± 0.124	0.392GluCys: 0.392 ± 0.02	1.934GluAsp: 1.934 ± 0.059	3.177GluGlu: 3.177 ± 0.074	1.222GluPhe: 1.222 ± 0.044	4.33GluGly: 4.33 ± 0.083	1.372GluHis: 1.372 ± 0.039	2.17GluIle: 2.17 ± 0.053	1.399GluLys: 1.399 ± 0.045	8.737GluLeu: 8.737 ± 0.13	0.893GluMet: 0.893 ± 0.036	1.011GluAsn: 1.011 ± 0.035	3.427GluPro: 3.427 ± 0.07	4.471GluGln: 4.471 ± 0.079	5.654GluArg: 5.654 ± 0.095	2.435GluSer: 2.435 ± 0.051	2.194GluThr: 2.194 ± 0.051	3.7GluVal: 3.7 ± 0.085	0.787GluTrp: 0.787 ± 0.033	0.609GluTyr: 0.609 ± 0.036	0.0GluXaa: 0.0 ± 0.0
Phe	3.068PheAla: 3.068 ± 0.072	0.488PheCys: 0.488 ± 0.022	1.863PheAsp: 1.863 ± 0.047	1.785PheGlu: 1.785 ± 0.053	0.982PhePhe: 0.982 ± 0.039	2.758PheGly: 2.758 ± 0.063	0.622PheHis: 0.622 ± 0.032	1.065PheIle: 1.065 ± 0.046	0.741PheLys: 0.741 ± 0.029	2.958PheLeu: 2.958 ± 0.058	0.494PheMet: 0.494 ± 0.028	1.159PheAsn: 1.159 ± 0.04	1.337PhePro: 1.337 ± 0.04	1.222PheGln: 1.222 ± 0.039	1.909PheArg: 1.909 ± 0.045	2.3PheSer: 2.3 ± 0.055	1.562PheThr: 1.562 ± 0.047	1.823PheVal: 1.823 ± 0.05	0.579PheTrp: 0.579 ± 0.026	0.717PheTyr: 0.717 ± 0.032	0.0PheXaa: 0.0 ± 0.0
Gly	8.073GlyAla: 8.073 ± 0.11	1.307GlyCys: 1.307 ± 0.044	4.401GlyAsp: 4.401 ± 0.078	4.511GlyGlu: 4.511 ± 0.08	3.092GlyPhe: 3.092 ± 0.068	7.356GlyGly: 7.356 ± 0.13	1.739GlyHis: 1.739 ± 0.051	3.676GlyIle: 3.676 ± 0.073	2.339GlyLys: 2.339 ± 0.071	11.14GlyLeu: 11.14 ± 0.151	1.983GlyMet: 1.983 ± 0.057	2.008GlyAsn: 2.008 ± 0.056	3.506GlyPro: 3.506 ± 0.067	3.695GlyGln: 3.695 ± 0.074	5.96GlyArg: 5.96 ± 0.091	5.813GlySer: 5.813 ± 0.101	3.921GlyThr: 3.921 ± 0.086	6.241GlyVal: 6.241 ± 0.097	2.018GlyTrp: 2.018 ± 0.061	2.001GlyTyr: 2.001 ± 0.049	0.0GlyXaa: 0.0 ± 0.0
His	1.886HisAla: 1.886 ± 0.044	0.399HisCys: 0.399 ± 0.026	0.912HisAsp: 0.912 ± 0.038	0.868HisGlu: 0.868 ± 0.033	0.662HisPhe: 0.662 ± 0.03	1.996HisGly: 1.996 ± 0.055	0.829HisHis: 0.829 ± 0.039	0.587HisIle: 0.587 ± 0.03	0.36HisLys: 0.36 ± 0.023	2.537HisLeu: 2.537 ± 0.057	0.226HisMet: 0.226 ± 0.016	0.571HisAsn: 0.571 ± 0.027	1.6HisPro: 1.6 ± 0.047	1.167HisGln: 1.167 ± 0.042	1.826HisArg: 1.826 ± 0.049	1.207HisSer: 1.207 ± 0.039	0.903HisThr: 0.903 ± 0.036	1.104HisVal: 1.104 ± 0.039	0.652HisTrp: 0.652 ± 0.03	0.54HisTyr: 0.54 ± 0.03	0.0HisXaa: 0.0 ± 0.0
Ile	4.845IleAla: 4.845 ± 0.092	0.506IleCys: 0.506 ± 0.027	2.451IleAsp: 2.451 ± 0.056	2.675IleGlu: 2.675 ± 0.056	0.924IlePhe: 0.924 ± 0.037	3.878IleGly: 3.878 ± 0.075	0.923IleHis: 0.923 ± 0.033	0.878IleIle: 0.878 ± 0.035	1.088IleLys: 1.088 ± 0.04	2.963IleLeu: 2.963 ± 0.066	0.313IleMet: 0.313 ± 0.017	1.393IleAsn: 1.393 ± 0.046	2.35IlePro: 2.35 ± 0.053	1.643IleGln: 1.643 ± 0.045	2.774IleArg: 2.774 ± 0.062	2.658IleSer: 2.658 ± 0.071	2.704IleThr: 2.704 ± 0.06	2.249IleVal: 2.249 ± 0.069	0.632IleTrp: 0.632 ± 0.03	0.78IleTyr: 0.78 ± 0.034	0.0IleXaa: 0.0 ± 0.0
Lys	3.106LysAla: 3.106 ± 0.076	0.153LysCys: 0.153 ± 0.015	1.021LysAsp: 1.021 ± 0.038	1.372LysGlu: 1.372 ± 0.049	0.52LysPhe: 0.52 ± 0.028	2.13LysGly: 2.13 ± 0.057	0.422LysHis: 0.422 ± 0.026	0.837LysIle: 0.837 ± 0.037	0.797LysLys: 0.797 ± 0.042	2.806LysLeu: 2.806 ± 0.066	0.279LysMet: 0.279 ± 0.018	0.55LysAsn: 0.55 ± 0.032	1.898LysPro: 1.898 ± 0.063	1.277LysGln: 1.277 ± 0.041	1.916LysArg: 1.916 ± 0.056	1.121LysSer: 1.121 ± 0.041	1.24LysThr: 1.24 ± 0.051	1.706LysVal: 1.706 ± 0.054	0.165LysTrp: 0.165 ± 0.014	0.4LysTyr: 0.4 ± 0.027	0.0LysXaa: 0.0 ± 0.0
Leu	15.503LeuAla: 15.503 ± 0.213	1.346LeuCys: 1.346 ± 0.042	6.068LeuAsp: 6.068 ± 0.098	9.17LeuGlu: 9.17 ± 0.133	3.432LeuPhe: 3.432 ± 0.074	9.95LeuGly: 9.95 ± 0.131	2.498LeuHis: 2.498 ± 0.055	5.283LeuIle: 5.283 ± 0.081	3.278LeuLys: 3.278 ± 0.076	17.278LeuLeu: 17.278 ± 0.241	2.487LeuMet: 2.487 ± 0.06	3.205LeuAsn: 3.205 ± 0.066	7.657LeuPro: 7.657 ± 0.13	7.346LeuGln: 7.346 ± 0.138	9.284LeuArg: 9.284 ± 0.117	7.634LeuSer: 7.634 ± 0.117	5.197LeuThr: 5.197 ± 0.101	9.624LeuVal: 9.624 ± 0.146	1.966LeuTrp: 1.966 ± 0.061	1.949LeuTyr: 1.949 ± 0.053	0.0LeuXaa: 0.0 ± 0.0
Met	3.116MetAla: 3.116 ± 0.061	0.092MetCys: 0.092 ± 0.012	0.782MetAsp: 0.782 ± 0.036	1.049MetGlu: 1.049 ± 0.04	0.327MetPhe: 0.327 ± 0.02	1.732MetGly: 1.732 ± 0.048	0.343MetHis: 0.343 ± 0.023	0.575MetIle: 0.575 ± 0.027	0.518MetLys: 0.518 ± 0.024	1.872MetLeu: 1.872 ± 0.045	0.202MetMet: 0.202 ± 0.017	0.455MetAsn: 0.455 ± 0.022	1.307MetPro: 1.307 ± 0.038	0.862MetGln: 0.862 ± 0.029	1.268MetArg: 1.268 ± 0.04	1.126MetSer: 1.126 ± 0.038	1.049MetThr: 1.049 ± 0.033	1.433MetVal: 1.433 ± 0.042	0.092MetTrp: 0.092 ± 0.01	0.183MetTyr: 0.183 ± 0.016	0.0MetXaa: 0.0 ± 0.0
Asn	2.669AsnAla: 2.669 ± 0.06	0.253AsnCys: 0.253 ± 0.019	1.207AsnAsp: 1.207 ± 0.042	0.975AsnGlu: 0.975 ± 0.039	0.752AsnPhe: 0.752 ± 0.035	2.371AsnGly: 2.371 ± 0.068	0.518AsnHis: 0.518 ± 0.027	0.803AsnIle: 0.803 ± 0.031	0.605AsnLys: 0.605 ± 0.03	3.054AsnLeu: 3.054 ± 0.066	0.302AsnMet: 0.302 ± 0.018	0.761AsnAsn: 0.761 ± 0.041	1.997AsnPro: 1.997 ± 0.057	1.119AsnGln: 1.119 ± 0.038	2.006AsnArg: 2.006 ± 0.044	1.394AsnSer: 1.394 ± 0.04	1.216AsnThr: 1.216 ± 0.046	1.414AsnVal: 1.414 ± 0.041	0.46AsnTrp: 0.46 ± 0.026	0.548AsnTyr: 0.548 ± 0.027	0.0AsnXaa: 0.0 ± 0.0
Pro	7.497ProAla: 7.497 ± 0.13	0.587ProCys: 0.587 ± 0.027	3.118ProAsp: 3.118 ± 0.067	4.349ProGlu: 4.349 ± 0.076	1.7ProPhe: 1.7 ± 0.048	4.897ProGly: 4.897 ± 0.08	1.132ProHis: 1.132 ± 0.042	2.141ProIle: 2.141 ± 0.043	1.206ProLys: 1.206 ± 0.044	7.529ProLeu: 7.529 ± 0.121	1.207ProMet: 1.207 ± 0.045	1.21ProAsn: 1.21 ± 0.04	3.2ProPro: 3.2 ± 0.09	2.854ProGln: 2.854 ± 0.061	3.099ProArg: 3.099 ± 0.06	3.622ProSer: 3.622 ± 0.064	2.529ProThr: 2.529 ± 0.052	4.222ProVal: 4.222 ± 0.074	1.206ProTrp: 1.206 ± 0.044	1.121ProTyr: 1.121 ± 0.038	0.0ProXaa: 0.0 ± 0.0
Gln	6.555GlnAla: 6.555 ± 0.099	0.465GlnCys: 0.465 ± 0.023	1.433GlnAsp: 1.433 ± 0.043	2.761GlnGlu: 2.761 ± 0.08	1.14GlnPhe: 1.14 ± 0.04	3.858GlnGly: 3.858 ± 0.072	1.12GlnHis: 1.12 ± 0.04	1.843GlnIle: 1.843 ± 0.051	1.029GlnLys: 1.029 ± 0.038	8.259GlnLeu: 8.259 ± 0.14	0.711GlnMet: 0.711 ± 0.032	0.836GlnAsn: 0.836 ± 0.038	3.49GlnPro: 3.49 ± 0.079	4.012GlnGln: 4.012 ± 0.108	5.217GlnArg: 5.217 ± 0.104	2.246GlnSer: 2.246 ± 0.056	1.586GlnThr: 1.586 ± 0.05	3.491GlnVal: 3.491 ± 0.07	0.938GlnTrp: 0.938 ± 0.036	0.511GlnTyr: 0.511 ± 0.025	0.0GlnXaa: 0.0 ± 0.0
Arg	6.09ArgAla: 6.09 ± 0.09	1.159ArgCys: 1.159 ± 0.036	3.392ArgAsp: 3.392 ± 0.073	3.969ArgGlu: 3.969 ± 0.085	2.809ArgPhe: 2.809 ± 0.061	4.85ArgGly: 4.85 ± 0.087	1.836ArgHis: 1.836 ± 0.051	3.172ArgIle: 3.172 ± 0.066	1.605ArgLys: 1.605 ± 0.047	10.066ArgLeu: 10.066 ± 0.139	1.468ArgMet: 1.468 ± 0.041	1.73ArgAsn: 1.73 ± 0.054	3.809ArgPro: 3.809 ± 0.077	4.495ArgGln: 4.495 ± 0.089	6.609ArgArg: 6.609 ± 0.128	4.993ArgSer: 4.993 ± 0.072	2.89ArgThr: 2.89 ± 0.066	4.413ArgVal: 4.413 ± 0.082	1.972ArgTrp: 1.972 ± 0.056	1.75ArgTyr: 1.75 ± 0.047	0.0ArgXaa: 0.0 ± 0.0
Ser	6.532SerAla: 6.532 ± 0.09	0.824SerCys: 0.824 ± 0.04	3.094SerAsp: 3.094 ± 0.067	3.084SerGlu: 3.084 ± 0.062	2.072SerPhe: 2.072 ± 0.052	6.095SerGly: 6.095 ± 0.086	1.266SerHis: 1.266 ± 0.04	2.23SerIle: 2.23 ± 0.052	1.364SerLys: 1.364 ± 0.044	7.174SerLeu: 7.174 ± 0.121	1.146SerMet: 1.146 ± 0.043	1.603SerAsn: 1.603 ± 0.043	3.217SerPro: 3.217 ± 0.068	2.405SerGln: 2.405 ± 0.054	4.238SerArg: 4.238 ± 0.08	4.323SerSer: 4.323 ± 0.086	3.158SerThr: 3.158 ± 0.065	3.705SerVal: 3.705 ± 0.072	1.224SerTrp: 1.224 ± 0.043	1.255SerTyr: 1.255 ± 0.043	0.0SerXaa: 0.0 ± 0.0
Thr	5.694ThrAla: 5.694 ± 0.088	0.469ThrCys: 0.469 ± 0.022	2.049ThrAsp: 2.049 ± 0.052	2.098ThrGlu: 2.098 ± 0.051	1.251ThrPhe: 1.251 ± 0.042	4.046ThrGly: 4.046 ± 0.091	0.847ThrHis: 0.847 ± 0.036	1.888ThrIle: 1.888 ± 0.049	0.968ThrLys: 0.968 ± 0.039	5.291ThrLeu: 5.291 ± 0.093	0.683ThrMet: 0.683 ± 0.03	1.147ThrAsn: 1.147 ± 0.046	3.459ThrPro: 3.459 ± 0.06	1.49ThrGln: 1.49 ± 0.038	2.458ThrArg: 2.458 ± 0.057	2.779ThrSer: 2.779 ± 0.066	2.638ThrThr: 2.638 ± 0.06	3.29ThrVal: 3.29 ± 0.067	0.686ThrTrp: 0.686 ± 0.03	1.103ThrTyr: 1.103 ± 0.041	0.0ThrXaa: 0.0 ± 0.0
Val	8.143ValAla: 8.143 ± 0.111	0.76ValCys: 0.76 ± 0.032	3.582ValAsp: 3.582 ± 0.066	4.278ValGlu: 4.278 ± 0.092	2.23ValPhe: 2.23 ± 0.064	5.385ValGly: 5.385 ± 0.09	1.256ValHis: 1.256 ± 0.04	3.274ValIle: 3.274 ± 0.066	1.751ValLys: 1.751 ± 0.052	9.329ValLeu: 9.329 ± 0.133	1.495ValMet: 1.495 ± 0.044	1.861ValAsn: 1.861 ± 0.051	3.545ValPro: 3.545 ± 0.071	3.053ValGln: 3.053 ± 0.058	4.187ValArg: 4.187 ± 0.077	3.982ValSer: 3.982 ± 0.075	2.822ValThr: 2.822 ± 0.074	6.555ValVal: 6.555 ± 0.109	0.965ValTrp: 0.965 ± 0.034	1.142ValTyr: 1.142 ± 0.044	0.0ValXaa: 0.0 ± 0.0
Trp	1.477TrpAla: 1.477 ± 0.045	0.293TrpCys: 0.293 ± 0.018	0.711TrpAsp: 0.711 ± 0.031	0.718TrpGlu: 0.718 ± 0.026	0.569TrpPhe: 0.569 ± 0.028	1.348TrpGly: 1.348 ± 0.044	0.413TrpHis: 0.413 ± 0.023	0.804TrpIle: 0.804 ± 0.035	0.408TrpLys: 0.408 ± 0.021	3.194TrpLeu: 3.194 ± 0.086	0.458TrpMet: 0.458 ± 0.025	0.491TrpAsn: 0.491 ± 0.024	1.056TrpPro: 1.056 ± 0.041	1.277TrpGln: 1.277 ± 0.042	1.615TrpArg: 1.615 ± 0.052	1.161TrpSer: 1.161 ± 0.042	0.604TrpThr: 0.604 ± 0.029	1.043TrpVal: 1.043 ± 0.032	0.466TrpTrp: 0.466 ± 0.028	0.298TrpTyr: 0.298 ± 0.019	0.0TrpXaa: 0.0 ± 0.0
Tyr	1.919TyrAla: 1.919 ± 0.049	0.244TyrCys: 0.244 ± 0.017	0.929TyrAsp: 0.929 ± 0.042	0.882TyrGlu: 0.882 ± 0.034	0.575TyrPhe: 0.575 ± 0.03	2.098TyrGly: 2.098 ± 0.053	0.347TyrHis: 0.347 ± 0.023	0.582TyrIle: 0.582 ± 0.024	0.447TyrLys: 0.447 ± 0.027	2.158TyrLeu: 2.158 ± 0.06	0.277TyrMet: 0.277 ± 0.018	0.491TyrAsn: 0.491 ± 0.026	1.068TyrPro: 1.068 ± 0.036	0.797TyrGln: 0.797 ± 0.031	1.761TyrArg: 1.761 ± 0.048	1.268TyrSer: 1.268 ± 0.038	0.806TyrThr: 0.806 ± 0.03	1.224TyrVal: 1.224 ± 0.037	0.38TyrTrp: 0.38 ± 0.018	0.419TyrTyr: 0.419 ± 0.026	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.001XaaIle: 0.001 ± 0.001	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.001XaaArg: 0.001 ± 0.001	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.002XaaXaa: 0.002 ± 0.002

Statistics based on 2840 proteins (801788 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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