Cyanophage BHS3

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Podoviridae; unclassified Podoviridae

Average proteome isoelectric point is 6.26

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 9 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2H4KIS7|A0A2H4KIS7_9CAUD Uncharacterized protein OS=Cyanophage BHS3 OX=2024348 PE=4 SV=1
MM1 pKa = 7.57NEE3 pKa = 3.57QTFPLEE9 pKa = 4.04QYY11 pKa = 10.89GITPEE16 pKa = 3.84AAQAFIDD23 pKa = 3.85YY24 pKa = 9.77LLSYY28 pKa = 10.16EE29 pKa = 4.47PIRR32 pKa = 11.84SRR34 pKa = 11.84FEE36 pKa = 4.43FIDD39 pKa = 3.17EE40 pKa = 3.9RR41 pKa = 11.84RR42 pKa = 11.84RR43 pKa = 11.84YY44 pKa = 9.05HH45 pKa = 6.36ALKK48 pKa = 10.01PLRR51 pKa = 11.84DD52 pKa = 3.25EE53 pKa = 4.34WGDD56 pKa = 3.51DD57 pKa = 3.6FEE59 pKa = 6.57SNFEE63 pKa = 4.03KK64 pKa = 10.62VRR66 pKa = 11.84EE67 pKa = 4.18RR68 pKa = 11.84FASLPPNLQGLYY80 pKa = 10.73DD81 pKa = 3.92NVDD84 pKa = 3.54GARR87 pKa = 11.84LLYY90 pKa = 10.1QQIKK94 pKa = 9.99KK95 pKa = 8.39EE96 pKa = 3.93QEE98 pKa = 3.62AAAEE102 pKa = 4.15AQQQQQQQASPPPTFDD118 pKa = 4.34RR119 pKa = 11.84GRR121 pKa = 11.84TSNDD125 pKa = 1.9ISAGVASAYY134 pKa = 9.6KK135 pKa = 10.15YY136 pKa = 10.01KK137 pKa = 10.7QSDD140 pKa = 3.75ILRR143 pKa = 11.84MPRR146 pKa = 11.84DD147 pKa = 3.34EE148 pKa = 4.4YY149 pKa = 11.0QRR151 pKa = 11.84QARR154 pKa = 11.84EE155 pKa = 3.49IQAAYY160 pKa = 10.6AEE162 pKa = 4.42GLVDD166 pKa = 5.74LSAA169 pKa = 5.45

Molecular weight: 19.62 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2H4KIV0|A0A2H4KIV0_9CAUD Uncharacterized protein OS=Cyanophage BHS3 OX=2024348 PE=4 SV=1
MM1 pKa = 7.08AAKK4 pKa = 9.81RR5 pKa = 11.84RR6 pKa = 11.84KK7 pKa = 8.07PSSYY11 pKa = 9.76IDD13 pKa = 3.46PNAIRR18 pKa = 11.84MMEE21 pKa = 4.04EE22 pKa = 3.71QQRR25 pKa = 11.84LADD28 pKa = 3.65EE29 pKa = 4.21ARR31 pKa = 11.84ARR33 pKa = 11.84EE34 pKa = 3.83QAFIEE39 pKa = 4.29QQTKK43 pKa = 7.62QQQDD47 pKa = 3.34AYY49 pKa = 10.79NNMLSVYY56 pKa = 7.69QQQIAALQNARR67 pKa = 11.84AEE69 pKa = 4.08QQRR72 pKa = 11.84YY73 pKa = 8.8IDD75 pKa = 4.25EE76 pKa = 4.4LMKK79 pKa = 10.67QQEE82 pKa = 4.49EE83 pKa = 4.06QNKK86 pKa = 9.64AVEE89 pKa = 4.21EE90 pKa = 3.85QRR92 pKa = 11.84ARR94 pKa = 11.84EE95 pKa = 3.88EE96 pKa = 3.57QLARR100 pKa = 11.84MQEE103 pKa = 3.9AEE105 pKa = 4.9RR106 pKa = 11.84IQQATRR112 pKa = 11.84AYY114 pKa = 10.59SIFDD118 pKa = 3.62EE119 pKa = 4.22RR120 pKa = 11.84RR121 pKa = 11.84KK122 pKa = 10.0LANQRR127 pKa = 11.84RR128 pKa = 11.84ATIFGNVLPPGYY140 pKa = 8.4TLYY143 pKa = 10.77RR144 pKa = 11.84GILGG148 pKa = 3.75

Molecular weight: 17.48 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

9

0

9

3109

71

800

345.4

38.61

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.816 ± 0.553

0.708 ± 0.126

6.143 ± 0.488

5.339 ± 0.862

4.246 ± 0.193

6.819 ± 0.55

1.254 ± 0.335

5.146 ± 0.405

3.57 ± 0.618

7.623 ± 0.259

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.316 ± 0.414

5.372 ± 0.6

4.953 ± 0.383

4.696 ± 0.912

6.787 ± 0.984

6.336 ± 0.976

8.009 ± 1.159

7.72 ± 0.611

1.383 ± 0.147

3.763 ± 0.262
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski