Sapajus apella (Brown-capped capuchin) (Cebus apella)
Average proteome isoelectric point is 6.64
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 49368 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A6J3HGH0|A0A6J3HGH0_SAPAP arrestin domain-containing protein 1 isoform X1 OS=Sapajus apella OX=9515 GN=ARRDC1 PE=3 SV=1
MM1 pKa = 7.62 TFTLDD6 pKa = 3.58 DD7 pKa = 3.74 GTGVLEE13 pKa = 4.69 AYY15 pKa = 11.0 LMDD18 pKa = 4.05 SDD20 pKa = 5.86 KK21 pKa = 10.89 FFQIPASEE29 pKa = 4.08 VLMDD33 pKa = 6.21 DD34 pKa = 5.09 DD35 pKa = 4.88 LQKK38 pKa = 11.56 SMDD41 pKa = 3.91 MIMDD45 pKa = 3.93 MFCPPGIKK53 pKa = 9.57 VDD55 pKa = 4.05 AYY57 pKa = 9.77 PWLEE61 pKa = 4.05 CLIKK65 pKa = 10.55 SYY67 pKa = 11.3 NVTNGTEE74 pKa = 3.94 NQICYY79 pKa = 10.31 QIFDD83 pKa = 3.7 TRR85 pKa = 11.84 VAEE88 pKa = 4.48 DD89 pKa = 3.65 VII91 pKa = 4.67
Molecular weight: 10.41 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.728
IPC2_protein 3.668
IPC_protein 3.643
Toseland 3.414
ProMoST 3.834
Dawson 3.656
Bjellqvist 3.821
Wikipedia 3.63
Rodwell 3.478
Grimsley 3.338
Solomon 3.63
Lehninger 3.579
Nozaki 3.783
DTASelect 4.037
Thurlkill 3.503
EMBOSS 3.63
Sillero 3.77
Patrickios 0.769
IPC_peptide 3.617
IPC2_peptide 3.732
IPC2.peptide.svr19 3.707
Protein with the highest isoelectric point:
>tr|A0A6J3JNK7|A0A6J3JNK7_SAPAP Alkaline ceramidase OS=Sapajus apella OX=9515 GN=ACER2 PE=3 SV=1
MM1 pKa = 7.1 RR2 pKa = 11.84 AKK4 pKa = 9.12 WRR6 pKa = 11.84 KK7 pKa = 9.1 KK8 pKa = 9.32 RR9 pKa = 11.84 MRR11 pKa = 11.84 RR12 pKa = 11.84 LKK14 pKa = 10.08 RR15 pKa = 11.84 KK16 pKa = 8.21 RR17 pKa = 11.84 RR18 pKa = 11.84 KK19 pKa = 8.46 MRR21 pKa = 11.84 QRR23 pKa = 11.84 SKK25 pKa = 11.41
Molecular weight: 3.46 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.517
IPC2_protein 11.213
IPC_protein 12.793
Toseland 12.969
ProMoST 13.451
Dawson 12.969
Bjellqvist 12.954
Wikipedia 13.437
Rodwell 12.705
Grimsley 12.998
Solomon 13.451
Lehninger 13.364
Nozaki 12.969
DTASelect 12.954
Thurlkill 12.969
EMBOSS 13.466
Sillero 12.969
Patrickios 12.427
IPC_peptide 13.466
IPC2_peptide 12.442
IPC2.peptide.svr19 9.142
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
20350
29018
49368
36383883
24
34567
737.0
82.1
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.956 ± 0.013
2.052 ± 0.008
4.927 ± 0.008
7.447 ± 0.016
3.399 ± 0.008
6.347 ± 0.013
2.582 ± 0.005
4.295 ± 0.01
5.951 ± 0.015
9.724 ± 0.013
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.126 ± 0.005
3.624 ± 0.008
6.317 ± 0.016
5.003 ± 0.013
5.671 ± 0.01
8.636 ± 0.013
5.376 ± 0.009
5.902 ± 0.009
1.13 ± 0.003
2.531 ± 0.007
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here