Mycoplasma canadense

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Tenericutes; Mollicutes; Mycoplasmatales; Mycoplasmataceae; Mycoplasma

Average proteome isoelectric point is 7.54

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 481 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A077L6F7|A0A077L6F7_9MOLU Oligopeptide ABC transporter OS=Mycoplasma canadense OX=29554 GN=oppB PE=3 SV=1
MM1 pKa = 7.79EE2 pKa = 5.27IKK4 pKa = 10.22KK5 pKa = 10.79DD6 pKa = 3.73NIKK9 pKa = 10.02KK10 pKa = 10.08QKK12 pKa = 10.53INICSSEE19 pKa = 3.91RR20 pKa = 11.84QIILEE25 pKa = 4.37NGDD28 pKa = 2.88IFYY31 pKa = 11.22VLFEE35 pKa = 4.59IDD37 pKa = 3.28EE38 pKa = 4.52NGEE41 pKa = 3.94HH42 pKa = 7.33FIALTDD48 pKa = 3.48KK49 pKa = 11.13KK50 pKa = 10.92SILFAKK56 pKa = 9.81IDD58 pKa = 3.81SKK60 pKa = 11.59NEE62 pKa = 3.57EE63 pKa = 4.1LVEE66 pKa = 4.22VEE68 pKa = 4.58DD69 pKa = 3.87EE70 pKa = 4.24AVIEE74 pKa = 4.13ILLDD78 pKa = 3.82LLDD81 pKa = 4.4EE82 pKa = 4.47FLEE85 pKa = 4.41NVDD88 pKa = 4.25IVDD91 pKa = 3.69EE92 pKa = 4.67KK93 pKa = 11.74GNDD96 pKa = 3.4LSKK99 pKa = 11.23LLLVDD104 pKa = 3.88QEE106 pKa = 4.42EE107 pKa = 4.55SS108 pKa = 3.2

Molecular weight: 12.48 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A077L9S8|A0A077L9S8_9MOLU DUF31 domain-containing protein OS=Mycoplasma canadense OX=29554 GN=MCAN360_0759 PE=4 SV=1
MM1 pKa = 7.73ADD3 pKa = 3.69KK4 pKa = 10.46IRR6 pKa = 11.84YY7 pKa = 8.14YY8 pKa = 11.18GLGRR12 pKa = 11.84RR13 pKa = 11.84KK14 pKa = 10.01SSVARR19 pKa = 11.84VYY21 pKa = 10.32LIPGKK26 pKa = 10.97GNFVINGKK34 pKa = 7.41EE35 pKa = 3.47ARR37 pKa = 11.84QYY39 pKa = 11.24LNSDD43 pKa = 3.47ILLKK47 pKa = 10.77DD48 pKa = 3.32ALSPFVVTEE57 pKa = 3.92TTNQFDD63 pKa = 3.01IFANVNGGGLTGQAGAIRR81 pKa = 11.84LGIARR86 pKa = 11.84GLLEE90 pKa = 4.47ASNNEE95 pKa = 3.78YY96 pKa = 10.57RR97 pKa = 11.84NKK99 pKa = 10.73LKK101 pKa = 10.82DD102 pKa = 3.29AGFLTRR108 pKa = 11.84DD109 pKa = 3.24ARR111 pKa = 11.84VKK113 pKa = 9.75EE114 pKa = 4.1RR115 pKa = 11.84KK116 pKa = 9.77KK117 pKa = 10.69FGLRR121 pKa = 11.84KK122 pKa = 9.46ARR124 pKa = 11.84RR125 pKa = 11.84ARR127 pKa = 11.84QFSKK131 pKa = 10.87RR132 pKa = 3.51

Molecular weight: 14.81 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

481

0

481

195216

99

3189

405.9

46.81

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

4.562 ± 0.104

0.494 ± 0.027

5.281 ± 0.077

7.491 ± 0.141

5.556 ± 0.115

4.197 ± 0.122

1.235 ± 0.036

10.578 ± 0.127

11.647 ± 0.125

9.587 ± 0.097

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.541 ± 0.045

8.868 ± 0.165

2.534 ± 0.063

3.07 ± 0.049

2.574 ± 0.058

6.591 ± 0.08

4.67 ± 0.061

4.638 ± 0.076

0.867 ± 0.034

4.02 ± 0.071
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski