Halomonas taeanensis
Average proteome isoelectric point is 6.0
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3429 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A1G7N2X4|A0A1G7N2X4_9GAMM Transcription elongation factor GreB OS=Halomonas taeanensis OX=284577 GN=greB PE=3 SV=1
MM1 pKa = 7.71 KK2 pKa = 9.06 KK3 pKa = 8.27 TLLATAIAGAMVVSASASAATVYY26 pKa = 10.79 NQDD29 pKa = 3.01 GTKK32 pKa = 10.43 LDD34 pKa = 3.43 IYY36 pKa = 11.42 GNIQMVYY43 pKa = 10.28 TNINDD48 pKa = 3.92 AAGDD52 pKa = 4.09 SEE54 pKa = 6.59 DD55 pKa = 4.82 EE56 pKa = 4.12 ITDD59 pKa = 3.32 NGTTFGFAAEE69 pKa = 4.18 HH70 pKa = 7.0 AITSDD75 pKa = 2.92 VTGYY79 pKa = 9.51 MKK81 pKa = 11.03 LEE83 pKa = 4.02 FDD85 pKa = 5.9 GIQADD90 pKa = 4.0 EE91 pKa = 4.36 KK92 pKa = 10.33 KK93 pKa = 7.5 TTGFDD98 pKa = 3.53 DD99 pKa = 5.33 GGDD102 pKa = 3.49 QAYY105 pKa = 10.47 VGAKK109 pKa = 10.24 GSFGDD114 pKa = 3.92 VRR116 pKa = 11.84 LGSYY120 pKa = 10.15 DD121 pKa = 3.62 QLIDD125 pKa = 4.93 DD126 pKa = 5.85 WIQDD130 pKa = 3.91 PITNNEE136 pKa = 3.93 YY137 pKa = 10.85 FDD139 pKa = 5.56 ASDD142 pKa = 3.34 STYY145 pKa = 11.57 DD146 pKa = 3.21 RR147 pKa = 11.84 TNDD150 pKa = 3.43 NNVGSIRR157 pKa = 11.84 EE158 pKa = 4.13 TDD160 pKa = 3.12 KK161 pKa = 10.77 VTYY164 pKa = 9.0 TSPSFGGLQFAVGSQYY180 pKa = 11.19 KK181 pKa = 10.58 GDD183 pKa = 4.13 AEE185 pKa = 4.44 DD186 pKa = 4.72 EE187 pKa = 4.32 NVTDD191 pKa = 3.5 SGEE194 pKa = 3.8 ASFFGGAKK202 pKa = 7.75 YY203 pKa = 8.77 TVGAFSIAAVYY214 pKa = 10.89 DD215 pKa = 3.63 NLDD218 pKa = 3.61 TFDD221 pKa = 3.67 ATTTPEE227 pKa = 3.92 TDD229 pKa = 3.57 FGDD232 pKa = 3.8 QYY234 pKa = 11.86 GVTAQYY240 pKa = 9.28 TAGALRR246 pKa = 11.84 LSAKK250 pKa = 9.44 YY251 pKa = 10.46 EE252 pKa = 3.96 KK253 pKa = 10.58 FDD255 pKa = 4.95 DD256 pKa = 4.83 GSSAEE261 pKa = 3.98 NDD263 pKa = 2.87 VDD265 pKa = 5.13 FAALGARR272 pKa = 11.84 YY273 pKa = 9.24 GYY275 pKa = 11.56 GMGDD279 pKa = 3.04 VYY281 pKa = 11.38 GAVQEE286 pKa = 4.23 VSYY289 pKa = 11.17 DD290 pKa = 3.27 EE291 pKa = 4.53 SGRR294 pKa = 11.84 DD295 pKa = 3.38 DD296 pKa = 3.59 RR297 pKa = 11.84 TEE299 pKa = 4.08 VIVGGTYY306 pKa = 10.41 NISSAMYY313 pKa = 9.16 TYY315 pKa = 10.54 IEE317 pKa = 4.31 VANYY321 pKa = 10.15 DD322 pKa = 3.98 ADD324 pKa = 3.67 EE325 pKa = 4.41 DD326 pKa = 4.69 AYY328 pKa = 11.16 DD329 pKa = 4.15 GVATGITYY337 pKa = 9.77 LFF339 pKa = 4.37
Molecular weight: 36.29 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.73
IPC2_protein 3.668
IPC_protein 3.706
Toseland 3.478
ProMoST 3.884
Dawson 3.706
Bjellqvist 3.872
Wikipedia 3.668
Rodwell 3.528
Grimsley 3.376
Solomon 3.706
Lehninger 3.668
Nozaki 3.821
DTASelect 4.101
Thurlkill 3.528
EMBOSS 3.668
Sillero 3.834
Patrickios 0.617
IPC_peptide 3.694
IPC2_peptide 3.808
IPC2.peptide.svr19 3.757
Protein with the highest isoelectric point:
>tr|A0A1G7S8V3|A0A1G7S8V3_9GAMM Iron complex transport system permease protein OS=Halomonas taeanensis OX=284577 GN=SAMN05216571_10624 PE=3 SV=1
MM1 pKa = 7.45 KK2 pKa = 9.51 RR3 pKa = 11.84 TFQPSVLKK11 pKa = 10.46 RR12 pKa = 11.84 KK13 pKa = 9.37 RR14 pKa = 11.84 NHH16 pKa = 5.37 GFRR19 pKa = 11.84 ARR21 pKa = 11.84 MATKK25 pKa = 10.3 NGRR28 pKa = 11.84 AVLARR33 pKa = 11.84 RR34 pKa = 11.84 RR35 pKa = 11.84 AKK37 pKa = 9.93 GRR39 pKa = 11.84 KK40 pKa = 8.85 RR41 pKa = 11.84 LSAA44 pKa = 3.96
Molecular weight: 5.16 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.523
IPC2_protein 11.257
IPC_protein 12.837
Toseland 13.013
ProMoST 13.495
Dawson 13.013
Bjellqvist 12.998
Wikipedia 13.481
Rodwell 12.705
Grimsley 13.042
Solomon 13.51
Lehninger 13.408
Nozaki 13.013
DTASelect 12.998
Thurlkill 13.013
EMBOSS 13.51
Sillero 13.013
Patrickios 12.427
IPC_peptide 13.51
IPC2_peptide 12.486
IPC2.peptide.svr19 9.173
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3429
0
3429
1106853
29
2858
322.8
35.33
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
11.401 ± 0.055
0.928 ± 0.013
6.004 ± 0.046
6.386 ± 0.041
3.378 ± 0.027
8.228 ± 0.041
2.392 ± 0.025
4.647 ± 0.032
2.862 ± 0.032
11.445 ± 0.056
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.534 ± 0.022
2.565 ± 0.022
4.864 ± 0.029
3.942 ± 0.028
7.003 ± 0.04
5.701 ± 0.03
4.961 ± 0.026
7.008 ± 0.035
1.424 ± 0.018
2.327 ± 0.018
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here