Beihai weivirus-like virus 8
Average proteome isoelectric point is 7.62
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A1L3KL95|A0A1L3KL95_9VIRU RNA-dependent RNA polymerase OS=Beihai weivirus-like virus 8 OX=1922755 PE=4 SV=1
MM1 pKa = 7.44 TLQLSRR7 pKa = 11.84 ANTTGIRR14 pKa = 11.84 DD15 pKa = 3.48 VEE17 pKa = 3.79 PWMFKK22 pKa = 9.95 IARR25 pKa = 11.84 EE26 pKa = 3.93 RR27 pKa = 11.84 PGMYY31 pKa = 9.09 TIKK34 pKa = 10.36 IFIPSMPDD42 pKa = 3.07 CPFDD46 pKa = 3.28 IAKK49 pKa = 10.41 AEE51 pKa = 4.14 SVMRR55 pKa = 11.84 KK56 pKa = 8.48 VLLYY60 pKa = 10.93 GSVHH64 pKa = 7.12 LLLCPVAHH72 pKa = 7.04 LMLGMPGFFYY82 pKa = 10.8 FLAVVNVIGFVFVYY96 pKa = 7.11 TQWPEE101 pKa = 4.24 TIGEE105 pKa = 4.22 TLEE108 pKa = 4.09 VNFTGEE114 pKa = 4.03 EE115 pKa = 3.89 FAKK118 pKa = 10.23 LDD120 pKa = 3.86 KK121 pKa = 11.24 VIMNQTYY128 pKa = 8.46 TEE130 pKa = 4.37 SKK132 pKa = 10.67 SKK134 pKa = 9.94 VQSAIVFQHH143 pKa = 6.72 ASVIWPSHH151 pKa = 5.47 TPDD154 pKa = 3.84 DD155 pKa = 3.72 VTMRR159 pKa = 11.84 TLGPWAITEE168 pKa = 4.07 YY169 pKa = 10.27 RR170 pKa = 11.84 CNGTWMKK177 pKa = 11.05 NFFKK181 pKa = 10.67 RR182 pKa = 11.84 FPYY185 pKa = 10.16 LPCGYY190 pKa = 10.64 SMKK193 pKa = 10.51 KK194 pKa = 7.81 QVRR197 pKa = 11.84 LCLRR201 pKa = 11.84 QGIGGATVTSKK212 pKa = 11.25 DD213 pKa = 3.08 GDD215 pKa = 3.94 LPEE218 pKa = 5.73 ADD220 pKa = 4.54 PTPKK224 pKa = 9.97 PAPPVDD230 pKa = 3.97 LSSDD234 pKa = 3.32 TGVPVDD240 pKa = 3.38 KK241 pKa = 10.56 RR242 pKa = 11.84 YY243 pKa = 10.68 VRR245 pKa = 11.84 TQDD248 pKa = 2.74 VSQIEE253 pKa = 4.25 RR254 pKa = 11.84 HH255 pKa = 4.51 AHH257 pKa = 5.17 KK258 pKa = 10.49 CCEE261 pKa = 4.13 CGCTYY266 pKa = 10.99 EE267 pKa = 4.56 HH268 pKa = 6.73 GHH270 pKa = 6.7 PGGHH274 pKa = 6.29 GKK276 pKa = 9.61 HH277 pKa = 5.53 GQCLGNCPEE286 pKa = 4.3 PTCPRR291 pKa = 11.84 HH292 pKa = 6.96 DD293 pKa = 4.37 PGVDD297 pKa = 3.17 YY298 pKa = 11.4 AGAQKK303 pKa = 10.42 LQPTPPKK310 pKa = 9.96 EE311 pKa = 4.15 EE312 pKa = 4.3 PKK314 pKa = 8.4 TTEE317 pKa = 3.91 SKK319 pKa = 10.68 PPRR322 pKa = 11.84 NDD324 pKa = 2.92 LGYY327 pKa = 10.48 VEE329 pKa = 5.4 PGHH332 pKa = 6.56 GNEE335 pKa = 4.92 DD336 pKa = 3.65 LHH338 pKa = 7.35 PLGGASAEE346 pKa = 4.14 DD347 pKa = 3.67 TEE349 pKa = 5.02 IRR351 pKa = 11.84 AEE353 pKa = 3.96 RR354 pKa = 11.84 QDD356 pKa = 4.01 RR357 pKa = 11.84 IVEE360 pKa = 4.1 NVAGVGVVGQSTDD373 pKa = 2.83 SSNTKK378 pKa = 10.19 QIVGVVSLPVTDD390 pKa = 4.0 TPNVYY395 pKa = 10.42 ARR397 pKa = 11.84 EE398 pKa = 4.04 AANVQSAIDD407 pKa = 3.49 NRR409 pKa = 11.84 ITKK412 pKa = 9.11 KK413 pKa = 10.28 QRR415 pKa = 11.84 PFTANKK421 pKa = 9.09 EE422 pKa = 4.14 DD423 pKa = 3.62 KK424 pKa = 10.72 ALLGRR429 pKa = 11.84 LVYY432 pKa = 10.32 AAIGDD437 pKa = 3.8 HH438 pKa = 6.34 PRR440 pKa = 11.84 RR441 pKa = 11.84 SLFSARR447 pKa = 11.84 RR448 pKa = 11.84 VTAWWEE454 pKa = 3.78 THH456 pKa = 6.39 LFADD460 pKa = 4.81 LKK462 pKa = 9.91 SGKK465 pKa = 6.74 WTEE468 pKa = 4.25 DD469 pKa = 2.98 RR470 pKa = 11.84 LSRR473 pKa = 11.84 AIEE476 pKa = 4.0 NLCGRR481 pKa = 11.84 IQPGFKK487 pKa = 9.98 LACDD491 pKa = 3.82 VKK493 pKa = 10.74 LEE495 pKa = 4.01 PMPEE499 pKa = 3.92 GKK501 pKa = 10.26 APRR504 pKa = 11.84 MLIADD509 pKa = 3.75 GDD511 pKa = 4.09 EE512 pKa = 4.48 GQVLALLTVCCIEE525 pKa = 5.12 DD526 pKa = 3.89 SIKK529 pKa = 10.83 KK530 pKa = 9.47 HH531 pKa = 5.8 LPKK534 pKa = 9.8 KK535 pKa = 8.18 TIKK538 pKa = 10.87 GLGKK542 pKa = 8.82 RR543 pKa = 11.84 AAMEE547 pKa = 3.73 RR548 pKa = 11.84 VAAEE552 pKa = 3.7 LRR554 pKa = 11.84 APKK557 pKa = 9.94 AAYY560 pKa = 10.58 SNTKK564 pKa = 7.57 TQSTQRR570 pKa = 11.84 TGNGKK575 pKa = 7.52 MLKK578 pKa = 9.3 PGVSIFEE585 pKa = 4.53 GDD587 pKa = 3.13 GSAWDD592 pKa = 4.16 TTCSIQLRR600 pKa = 11.84 DD601 pKa = 3.75 CVEE604 pKa = 4.01 NPVILHH610 pKa = 5.91 VGSILKK616 pKa = 10.24 VFMAQPQSWIQAHH629 pKa = 7.04 DD630 pKa = 5.0 DD631 pKa = 3.31 ISVLDD636 pKa = 3.94 KK637 pKa = 10.86 LTITFKK643 pKa = 11.2 KK644 pKa = 9.7 NGEE647 pKa = 4.05 FRR649 pKa = 11.84 KK650 pKa = 10.28 HH651 pKa = 6.4 IIDD654 pKa = 4.97 AIRR657 pKa = 11.84 RR658 pKa = 11.84 SGHH661 pKa = 6.55 RR662 pKa = 11.84 GTSCLNWWINFTCWHH677 pKa = 6.1 CAIFEE682 pKa = 4.16 EE683 pKa = 5.05 PEE685 pKa = 4.13 KK686 pKa = 10.94 FLDD689 pKa = 3.52 PDD691 pKa = 3.12 VRR693 pKa = 11.84 YY694 pKa = 10.4 GRR696 pKa = 11.84 DD697 pKa = 2.88 HH698 pKa = 7.03 AGTYY702 pKa = 9.42 RR703 pKa = 11.84 WIASAFEE710 pKa = 4.57 GDD712 pKa = 4.43 DD713 pKa = 4.47 SILSTTPRR721 pKa = 11.84 IEE723 pKa = 4.44 EE724 pKa = 3.81 KK725 pKa = 10.91 DD726 pKa = 3.52 EE727 pKa = 4.95 LYY729 pKa = 11.05 VSLMQRR735 pKa = 11.84 WEE737 pKa = 3.86 RR738 pKa = 11.84 FGFNMKK744 pKa = 9.92 IFIRR748 pKa = 11.84 AKK750 pKa = 8.55 RR751 pKa = 11.84 ALFTGYY757 pKa = 10.67 HH758 pKa = 5.56 MALDD762 pKa = 3.98 NDD764 pKa = 4.25 GPTGVLMPEE773 pKa = 3.99 VDD775 pKa = 3.19 RR776 pKa = 11.84 CFARR780 pKa = 11.84 AGISCSPSMIEE791 pKa = 3.86 YY792 pKa = 9.88 FKK794 pKa = 11.21 KK795 pKa = 10.47 GDD797 pKa = 3.46 RR798 pKa = 11.84 AGCQSVSRR806 pKa = 11.84 AAALSRR812 pKa = 11.84 AHH814 pKa = 6.76 EE815 pKa = 4.32 FAGCAPTISTKK826 pKa = 7.95 YY827 pKa = 9.13 LRR829 pKa = 11.84 YY830 pKa = 9.77 YY831 pKa = 10.52 EE832 pKa = 4.14 SLAVKK837 pKa = 9.74 TNVDD841 pKa = 3.15 RR842 pKa = 11.84 DD843 pKa = 3.55 LQMRR847 pKa = 11.84 TCGGDD852 pKa = 3.37 AEE854 pKa = 4.64 FSEE857 pKa = 4.62 PDD859 pKa = 2.99 IVAEE863 pKa = 4.09 INLKK867 pKa = 10.53 NGAAMTFDD875 pKa = 3.05 SAEE878 pKa = 4.14 RR879 pKa = 11.84 DD880 pKa = 3.12 RR881 pKa = 11.84 LAAVGFEE888 pKa = 4.25 CTEE891 pKa = 4.09 EE892 pKa = 3.99 EE893 pKa = 4.32 LSRR896 pKa = 11.84 FSLRR900 pKa = 11.84 VWDD903 pKa = 3.96 YY904 pKa = 11.89 DD905 pKa = 4.16 VLKK908 pKa = 10.32 DD909 pKa = 3.35 WEE911 pKa = 4.63 GFRR914 pKa = 11.84 EE915 pKa = 4.15 SLPEE919 pKa = 3.87 SWRR922 pKa = 11.84 MAA924 pKa = 3.76
Molecular weight: 103.4 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 6.503
IPC2_protein 6.62
IPC_protein 6.59
Toseland 6.576
ProMoST 7.117
Dawson 7.117
Bjellqvist 7.263
Wikipedia 7.088
Rodwell 7.132
Grimsley 6.664
Solomon 7.132
Lehninger 7.146
Nozaki 7.6
DTASelect 7.439
Thurlkill 7.483
EMBOSS 7.454
Sillero 7.614
Patrickios 4.317
IPC_peptide 7.146
IPC2_peptide 7.073
IPC2.peptide.svr19 6.883
Protein with the highest isoelectric point:
>tr|A0A1L3KL95|A0A1L3KL95_9VIRU RNA-dependent RNA polymerase OS=Beihai weivirus-like virus 8 OX=1922755 PE=4 SV=1
MM1 pKa = 7.72 ALTAKK6 pKa = 7.91 QQRR9 pKa = 11.84 AIADD13 pKa = 3.55 AAPAAKK19 pKa = 10.06 AAMRR23 pKa = 11.84 AAFALQNRR31 pKa = 11.84 GRR33 pKa = 11.84 VPGVDD38 pKa = 3.35 RR39 pKa = 11.84 NAKK42 pKa = 6.97 QARR45 pKa = 11.84 ARR47 pKa = 11.84 GRR49 pKa = 11.84 RR50 pKa = 11.84 GIPTAIQNMPRR61 pKa = 11.84 GAAGPRR67 pKa = 11.84 GEE69 pKa = 4.8 MPDD72 pKa = 2.99 ATRR75 pKa = 11.84 FHH77 pKa = 7.45 WDD79 pKa = 3.11 QTPASSNVVAPRR91 pKa = 11.84 GFGYY95 pKa = 10.95 YY96 pKa = 10.19 DD97 pKa = 4.28 AFTHH101 pKa = 6.05 SPSDD105 pKa = 3.23 AVTAFSVGPATPIQANTRR123 pKa = 11.84 ANVTTSAPDD132 pKa = 3.55 GVGAGDD138 pKa = 4.82 LGLSMIIVYY147 pKa = 8.74 PAATDD152 pKa = 3.39 AQAALFKK159 pKa = 10.84 CGATATDD166 pKa = 4.4 PVTTTYY172 pKa = 10.19 FRR174 pKa = 11.84 SPQLALSSGPEE185 pKa = 3.72 SVMATRR191 pKa = 11.84 CSLQIRR197 pKa = 11.84 NITQVLNQGGVVRR210 pKa = 11.84 SLRR213 pKa = 11.84 VTTGIINPGIVNGPTQDD230 pKa = 4.15 LLDD233 pKa = 4.2 LAEE236 pKa = 4.38 HH237 pKa = 5.92 VRR239 pKa = 11.84 NHH241 pKa = 5.33 ARR243 pKa = 11.84 TRR245 pKa = 11.84 TYY247 pKa = 11.03 AGADD251 pKa = 3.22 LVEE254 pKa = 4.47 NKK256 pKa = 9.88 QINCTVVDD264 pKa = 3.76 QSRR267 pKa = 11.84 ATTFLDD273 pKa = 3.77 FGLQVPVEE281 pKa = 4.35 DD282 pKa = 5.27 LPWIPPGSGVSVGPYY297 pKa = 9.54 TLGLHH302 pKa = 6.07 NPTYY306 pKa = 10.54 TPIILLFEE314 pKa = 4.51 SFSTTNEE321 pKa = 3.7 YY322 pKa = 10.47 EE323 pKa = 3.71 ISIRR327 pKa = 11.84 TQFLAHH333 pKa = 5.8 YY334 pKa = 8.64 VQGSMLANLAITPPSVGDD352 pKa = 3.51 KK353 pKa = 11.21 LNVHH357 pKa = 7.06 RR358 pKa = 11.84 DD359 pKa = 3.11 NEE361 pKa = 4.11 EE362 pKa = 3.76 RR363 pKa = 11.84 KK364 pKa = 10.44 GSVLHH369 pKa = 6.61 DD370 pKa = 3.2 VSVALKK376 pKa = 10.49 HH377 pKa = 5.8 AGNWAMEE384 pKa = 4.25 HH385 pKa = 6.39 GGEE388 pKa = 4.03 IMGGIGAAKK397 pKa = 8.73 WAVPRR402 pKa = 11.84 VGQALGWGAKK412 pKa = 8.94 LAPLLL417 pKa = 4.31
Molecular weight: 44.19 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 8.736
IPC2_protein 8.609
IPC_protein 8.653
Toseland 9.048
ProMoST 9.004
Dawson 9.399
Bjellqvist 9.268
Wikipedia 9.604
Rodwell 9.487
Grimsley 9.458
Solomon 9.502
Lehninger 9.458
Nozaki 9.311
DTASelect 9.194
Thurlkill 9.253
EMBOSS 9.516
Sillero 9.414
Patrickios 4.838
IPC_peptide 9.487
IPC2_peptide 8.112
IPC2.peptide.svr19 7.713
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2
0
2
1341
417
924
670.5
73.79
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
9.545 ± 1.859
2.237 ± 0.726
5.593 ± 0.496
5.444 ± 1.228
3.729 ± 0.407
8.054 ± 0.622
2.535 ± 0.066
5.071 ± 0.131
4.922 ± 1.208
7.159 ± 0.132
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.461 ± 0.145
3.43 ± 0.539
6.264 ± 0.33
3.505 ± 0.503
6.562 ± 0.042
5.742 ± 0.108
6.786 ± 0.54
6.786 ± 0.425
1.715 ± 0.247
2.461 ± 0.145
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here