Chicory yellow mottle virus large satellite RNA
Average proteome isoelectric point is 9.16
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 1 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|Q04325|Q04325_9VIRU Uncharacterized protein OS=Chicory yellow mottle virus large satellite RNA OX=192021 PE=4 SV=1
MM1 pKa = 7.44 ARR3 pKa = 11.84 TVIIHH8 pKa = 6.6 AVASTRR14 pKa = 11.84 SVTASPTTANGRR26 pKa = 11.84 GRR28 pKa = 11.84 ASRR31 pKa = 11.84 KK32 pKa = 6.92 CTKK35 pKa = 10.48 NNAAVEE41 pKa = 4.22 SSRR44 pKa = 11.84 PRR46 pKa = 11.84 KK47 pKa = 9.16 LQRR50 pKa = 11.84 TAPIKK55 pKa = 10.1 TGTRR59 pKa = 11.84 DD60 pKa = 3.24 NRR62 pKa = 11.84 SYY64 pKa = 11.3 AQVVMEE70 pKa = 4.57 NCHH73 pKa = 6.59 HH74 pKa = 5.83 SHH76 pKa = 5.66 QSKK79 pKa = 10.37 ARR81 pKa = 11.84 SSEE84 pKa = 3.84 KK85 pKa = 10.08 FVAAKK90 pKa = 10.01 KK91 pKa = 9.21 VARR94 pKa = 11.84 PSVAVPSKK102 pKa = 10.1 LTTGNKK108 pKa = 9.45 FDD110 pKa = 3.41 VLGFEE115 pKa = 4.15 RR116 pKa = 11.84 HH117 pKa = 5.95 AIDD120 pKa = 3.43 RR121 pKa = 11.84 AVATSVPTKK130 pKa = 10.36 SVQEE134 pKa = 4.15 VVSVQKK140 pKa = 9.64 GTQYY144 pKa = 10.78 VVNHH148 pKa = 6.63 TGHH151 pKa = 6.68 SYY153 pKa = 9.88 GCSRR157 pKa = 11.84 KK158 pKa = 10.05 GNYY161 pKa = 10.04 ASTSYY166 pKa = 10.35 RR167 pKa = 11.84 CCAKK171 pKa = 10.36 VVEE174 pKa = 4.45 EE175 pKa = 4.14 PVMEE179 pKa = 4.52 KK180 pKa = 9.31 KK181 pKa = 8.82 TVFTDD186 pKa = 3.71 VPDD189 pKa = 3.81 EE190 pKa = 4.09 AAEE193 pKa = 4.09 LLRR196 pKa = 11.84 QEE198 pKa = 5.39 FLAKK202 pKa = 9.6 MPQLGHH208 pKa = 6.3 SVTLCPVGVDD218 pKa = 3.08 TLAWGNCSEE227 pKa = 4.87 LDD229 pKa = 3.53 SAVAAKK235 pKa = 9.02 PAWFGVPVKK244 pKa = 8.31 QQRR247 pKa = 11.84 KK248 pKa = 8.56 KK249 pKa = 9.64 MSRR252 pKa = 11.84 RR253 pKa = 11.84 DD254 pKa = 3.56 LRR256 pKa = 11.84 NLLSALRR263 pKa = 11.84 LARR266 pKa = 11.84 TTVNKK271 pKa = 10.41 SCSLGDD277 pKa = 3.29 AFMWAWRR284 pKa = 11.84 TLGFGGAAPLPDD296 pKa = 3.6 LKK298 pKa = 10.89 GWEE301 pKa = 3.94 LSHH304 pKa = 8.28 RR305 pKa = 11.84 EE306 pKa = 3.72 EE307 pKa = 3.99 RR308 pKa = 11.84 AAAPLHH314 pKa = 6.11 MYY316 pKa = 10.39 VLVIRR321 pKa = 11.84 CGGVGFSLWTGCFDD335 pKa = 4.73 APGKK339 pKa = 9.73 FIPAMVFHH347 pKa = 6.86 GFLLPLSYY355 pKa = 10.4 SWQSLEE361 pKa = 3.93 VV362 pKa = 3.37
Molecular weight: 39.66 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.16
IPC2_protein 9.18
IPC_protein 9.165
Toseland 10.101
ProMoST 9.692
Dawson 10.248
Bjellqvist 9.911
Wikipedia 10.379
Rodwell 10.73
Grimsley 10.292
Solomon 10.277
Lehninger 10.248
Nozaki 10.175
DTASelect 9.867
Thurlkill 10.131
EMBOSS 10.496
Sillero 10.175
Patrickios 10.394
IPC_peptide 10.277
IPC2_peptide 8.785
IPC2.peptide.svr19 8.062
Protein with the highest isoelectric point:
>tr|Q04325|Q04325_9VIRU Uncharacterized protein OS=Chicory yellow mottle virus large satellite RNA OX=192021 PE=4 SV=1
MM1 pKa = 7.44 ARR3 pKa = 11.84 TVIIHH8 pKa = 6.6 AVASTRR14 pKa = 11.84 SVTASPTTANGRR26 pKa = 11.84 GRR28 pKa = 11.84 ASRR31 pKa = 11.84 KK32 pKa = 6.92 CTKK35 pKa = 10.48 NNAAVEE41 pKa = 4.22 SSRR44 pKa = 11.84 PRR46 pKa = 11.84 KK47 pKa = 9.16 LQRR50 pKa = 11.84 TAPIKK55 pKa = 10.1 TGTRR59 pKa = 11.84 DD60 pKa = 3.24 NRR62 pKa = 11.84 SYY64 pKa = 11.3 AQVVMEE70 pKa = 4.57 NCHH73 pKa = 6.59 HH74 pKa = 5.83 SHH76 pKa = 5.66 QSKK79 pKa = 10.37 ARR81 pKa = 11.84 SSEE84 pKa = 3.84 KK85 pKa = 10.08 FVAAKK90 pKa = 10.01 KK91 pKa = 9.21 VARR94 pKa = 11.84 PSVAVPSKK102 pKa = 10.1 LTTGNKK108 pKa = 9.45 FDD110 pKa = 3.41 VLGFEE115 pKa = 4.15 RR116 pKa = 11.84 HH117 pKa = 5.95 AIDD120 pKa = 3.43 RR121 pKa = 11.84 AVATSVPTKK130 pKa = 10.36 SVQEE134 pKa = 4.15 VVSVQKK140 pKa = 9.64 GTQYY144 pKa = 10.78 VVNHH148 pKa = 6.63 TGHH151 pKa = 6.68 SYY153 pKa = 9.88 GCSRR157 pKa = 11.84 KK158 pKa = 10.05 GNYY161 pKa = 10.04 ASTSYY166 pKa = 10.35 RR167 pKa = 11.84 CCAKK171 pKa = 10.36 VVEE174 pKa = 4.45 EE175 pKa = 4.14 PVMEE179 pKa = 4.52 KK180 pKa = 9.31 KK181 pKa = 8.82 TVFTDD186 pKa = 3.71 VPDD189 pKa = 3.81 EE190 pKa = 4.09 AAEE193 pKa = 4.09 LLRR196 pKa = 11.84 QEE198 pKa = 5.39 FLAKK202 pKa = 9.6 MPQLGHH208 pKa = 6.3 SVTLCPVGVDD218 pKa = 3.08 TLAWGNCSEE227 pKa = 4.87 LDD229 pKa = 3.53 SAVAAKK235 pKa = 9.02 PAWFGVPVKK244 pKa = 8.31 QQRR247 pKa = 11.84 KK248 pKa = 8.56 KK249 pKa = 9.64 MSRR252 pKa = 11.84 RR253 pKa = 11.84 DD254 pKa = 3.56 LRR256 pKa = 11.84 NLLSALRR263 pKa = 11.84 LARR266 pKa = 11.84 TTVNKK271 pKa = 10.41 SCSLGDD277 pKa = 3.29 AFMWAWRR284 pKa = 11.84 TLGFGGAAPLPDD296 pKa = 3.6 LKK298 pKa = 10.89 GWEE301 pKa = 3.94 LSHH304 pKa = 8.28 RR305 pKa = 11.84 EE306 pKa = 3.72 EE307 pKa = 3.99 RR308 pKa = 11.84 AAAPLHH314 pKa = 6.11 MYY316 pKa = 10.39 VLVIRR321 pKa = 11.84 CGGVGFSLWTGCFDD335 pKa = 4.73 APGKK339 pKa = 9.73 FIPAMVFHH347 pKa = 6.86 GFLLPLSYY355 pKa = 10.4 SWQSLEE361 pKa = 3.93 VV362 pKa = 3.37
Molecular weight: 39.66 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.16
IPC2_protein 9.18
IPC_protein 9.165
Toseland 10.101
ProMoST 9.692
Dawson 10.248
Bjellqvist 9.911
Wikipedia 10.379
Rodwell 10.73
Grimsley 10.292
Solomon 10.277
Lehninger 10.248
Nozaki 10.175
DTASelect 9.867
Thurlkill 10.131
EMBOSS 10.496
Sillero 10.175
Patrickios 10.394
IPC_peptide 10.277
IPC2_peptide 8.785
IPC2.peptide.svr19 8.062
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
1
0
1
362
362
362
362.0
39.66
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
10.773 ± 0.0
2.762 ± 0.0
3.039 ± 0.0
4.42 ± 0.0
3.591 ± 0.0
6.63 ± 0.0
3.039 ± 0.0
1.657 ± 0.0
6.63 ± 0.0
7.459 ± 0.0
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.21 ± 0.0
3.039 ± 0.0
4.972 ± 0.0
3.039 ± 0.0
7.459 ± 0.0
8.84 ± 0.0
6.63 ± 0.0
9.945 ± 0.0
1.934 ± 0.0
1.934 ± 0.0
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here