Proteome-pI 2.0 - Proteome Isoelectric Point Database

Alternanthera yellow vein betasatellite

Taxonomy: Viruses; Tolecusatellitidae; Betasatellite

Average proteome isoelectric point is 4.92

Get precalculated fractions of proteins

Acidic

pI < 6.8

6.8-7.4

pI > 7.4

Basic

All

Virtual 2D-PAGE plot for 1 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions

Protein with the lowest isoelectric point:

>tr|A5H1G0|A5H1G0_9VIRU C1 protein OS=Alternanthera yellow vein betasatellite OX=447606 GN=C1 PE=4 SV=1

MM1 pKa = 7.23TIKK4 pKa = 10.82YY5 pKa = 9.38NNKK8 pKa = 9.72KK9 pKa = 10.34GMEE12 pKa = 4.37FIINVKK18 pKa = 10.29LKK20 pKa = 9.15EE21 pKa = 4.38DD22 pKa = 3.44NSIVVQIEE30 pKa = 4.42LISTKK35 pKa = 10.57SPALAKK41 pKa = 10.17KK42 pKa = 10.06IYY44 pKa = 8.31TISYY48 pKa = 7.95AHH50 pKa = 6.74SGIIPPFNFNNLEE63 pKa = 3.86EE64 pKa = 4.97GIRR67 pKa = 11.84NLLRR71 pKa = 11.84IMYY74 pKa = 9.37KK75 pKa = 10.15EE76 pKa = 3.66STIRR80 pKa = 11.84DD81 pKa = 4.08FKK83 pKa = 11.11QEE85 pKa = 4.48DD86 pKa = 3.8MVQTIDD92 pKa = 3.2ILMMQEE98 pKa = 3.74AAVEE102 pKa = 4.43DD103 pKa = 3.96IEE105 pKa = 6.04IEE107 pKa = 4.06DD108 pKa = 4.11EE109 pKa = 4.27YY110 pKa = 11.46GIGTIASII118 pKa = 4.47

Molecular weight: 13.53 kDa
Isoelectric point according different methods:

Protein with the highest isoelectric point:

>tr|A5H1G0|A5H1G0_9VIRU C1 protein OS=Alternanthera yellow vein betasatellite OX=447606 GN=C1 PE=4 SV=1

MM1 pKa = 7.23TIKK4 pKa = 10.82YY5 pKa = 9.38NNKK8 pKa = 9.72KK9 pKa = 10.34GMEE12 pKa = 4.37FIINVKK18 pKa = 10.29LKK20 pKa = 9.15EE21 pKa = 4.38DD22 pKa = 3.44NSIVVQIEE30 pKa = 4.42LISTKK35 pKa = 10.57SPALAKK41 pKa = 10.17KK42 pKa = 10.06IYY44 pKa = 8.31TISYY48 pKa = 7.95AHH50 pKa = 6.74SGIIPPFNFNNLEE63 pKa = 3.86EE64 pKa = 4.97GIRR67 pKa = 11.84NLLRR71 pKa = 11.84IMYY74 pKa = 9.37KK75 pKa = 10.15EE76 pKa = 3.66STIRR80 pKa = 11.84DD81 pKa = 4.08FKK83 pKa = 11.11QEE85 pKa = 4.48DD86 pKa = 3.8MVQTIDD92 pKa = 3.2ILMMQEE98 pKa = 3.74AAVEE102 pKa = 4.43DD103 pKa = 3.96IEE105 pKa = 6.04IEE107 pKa = 4.06DD108 pKa = 4.11EE109 pKa = 4.27YY110 pKa = 11.46GIGTIASII118 pKa = 4.47

Molecular weight: 13.53 kDa
Isoelectric point according different methods:

Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try ESI

ChTry ESI

ArgC ESI

LysN ESI

TryLysC ESI

Try MALDI

ChTry MALDI

ArgC MALDI

LysN MALDI

TryLysC MALDI

Try LTQ

ChTry LTQ

ArgC LTQ

LysN LTQ

TryLysC LTQ

Try MSlow

ChTry MSlow

ArgC MSlow

LysN MSlow

TryLysC MSlow

Try MShigh

ChTry MShigh

ArgC MShigh

LysN MShigh

TryLysC MShigh

General Statistics

Number of major isoforms	Number of additional isoforms	Number of all proteins	Number of amino acids	Min. Seq. Length	Max. Seq. Length	Avg. Seq. Length	Avg. Mol. Weight
1	0	1	118	118	118	118.0	13.53

Amino acid frequency

Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu
5.085 ± 0.0	0.0 ± 0.0	5.085 ± 0.0	10.169 ± 0.0	3.39 ± 0.0	4.237 ± 0.0	0.847 ± 0.0	16.949 ± 0.0	8.475 ± 0.0	5.932 ± 0.0

Met	Asn	Gln	Pro	Arg	Ser	Thr	Val	Trp	Tyr
5.085 ± 0.0	6.78 ± 0.0	2.542 ± 0.0	3.39 ± 0.0	2.542 ± 0.0	5.932 ± 0.0	5.085 ± 0.0	4.237 ± 0.0	0.0 ± 0.0	4.237 ± 0.0

Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here

See this proteome in:

Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski