Pantoea phage vB_PagS_AAS23
Average proteome isoelectric point is 6.69
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 92 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A3S9U7V1|A0A3S9U7V1_9CAUD Uncharacterized protein OS=Pantoea phage vB_PagS_AAS23 OX=2499073 GN=AAS23_gp91 PE=4 SV=1
MM1 pKa = 7.91 EE2 pKa = 4.46 IQEE5 pKa = 4.31 LNEE8 pKa = 3.8 DD9 pKa = 3.95 LMWILFQGMVCTALVDD25 pKa = 3.85 AGFNGDD31 pKa = 3.68 AWNMASCLDD40 pKa = 3.68 MSFDD44 pKa = 4.01 DD45 pKa = 4.07 ARR47 pKa = 11.84 DD48 pKa = 3.58 YY49 pKa = 11.5 AVEE52 pKa = 4.08 DD53 pKa = 3.12 WGSIIKK59 pKa = 10.5 EE60 pKa = 3.92 EE61 pKa = 4.0 LNEE64 pKa = 3.84 YY65 pKa = 10.49 RR66 pKa = 11.84 EE67 pKa = 4.23 EE68 pKa = 4.23 NPNSFSNLHH77 pKa = 5.76
Molecular weight: 8.9 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.791
IPC2_protein 3.706
IPC_protein 3.617
Toseland 3.439
ProMoST 3.783
Dawson 3.617
Bjellqvist 3.783
Wikipedia 3.554
Rodwell 3.465
Grimsley 3.35
Solomon 3.579
Lehninger 3.541
Nozaki 3.745
DTASelect 3.91
Thurlkill 3.503
EMBOSS 3.554
Sillero 3.745
Patrickios 1.825
IPC_peptide 3.579
IPC2_peptide 3.719
IPC2.peptide.svr19 3.716
Protein with the highest isoelectric point:
>tr|A0A3S9U7Q6|A0A3S9U7Q6_9CAUD Putative phosphoesterase OS=Pantoea phage vB_PagS_AAS23 OX=2499073 GN=AAS23_gp38 PE=4 SV=1
MM1 pKa = 7.57 SEE3 pKa = 4.54 RR4 pKa = 11.84 IAVSTGEE11 pKa = 3.73 PDD13 pKa = 3.71 KK14 pKa = 11.2 RR15 pKa = 11.84 PKK17 pKa = 10.43 NGNNGTRR24 pKa = 11.84 RR25 pKa = 11.84 GPDD28 pKa = 2.73 KK29 pKa = 10.72 RR30 pKa = 11.84 PRR32 pKa = 11.84 KK33 pKa = 9.54 RR34 pKa = 11.84 CIGYY38 pKa = 9.78 DD39 pKa = 3.16 ILKK42 pKa = 10.62 DD43 pKa = 3.63 EE44 pKa = 4.9 VKK46 pKa = 10.81 ASLANRR52 pKa = 11.84 LQTVLAYY59 pKa = 10.33 YY60 pKa = 7.55 GTKK63 pKa = 10.59 AEE65 pKa = 3.95 MGRR68 pKa = 11.84 RR69 pKa = 11.84 LKK71 pKa = 10.82 VSKK74 pKa = 8.9 QTINAWIMRR83 pKa = 11.84 GRR85 pKa = 11.84 ISTEE89 pKa = 3.44 GARR92 pKa = 11.84 RR93 pKa = 11.84 VHH95 pKa = 6.88 RR96 pKa = 11.84 DD97 pKa = 3.17 YY98 pKa = 11.19 IRR100 pKa = 11.84 NGFSGYY106 pKa = 9.51 RR107 pKa = 11.84 ASFCRR112 pKa = 11.84 PDD114 pKa = 3.91 LRR116 pKa = 11.84 FDD118 pKa = 4.13 ANGKK122 pKa = 9.47 AVSQRR127 pKa = 11.84 CNRR130 pKa = 11.84 KK131 pKa = 10.45 DD132 pKa = 3.31 MVKK135 pKa = 10.77
Molecular weight: 15.46 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.327
IPC2_protein 9.853
IPC_protein 10.818
Toseland 10.994
ProMoST 10.877
Dawson 11.052
Bjellqvist 10.818
Wikipedia 11.316
Rodwell 11.213
Grimsley 11.096
Solomon 11.257
Lehninger 11.213
Nozaki 10.979
DTASelect 10.818
Thurlkill 10.979
EMBOSS 11.418
Sillero 10.994
Patrickios 10.935
IPC_peptide 11.257
IPC2_peptide 9.955
IPC2.peptide.svr19 8.567
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
92
0
92
16002
27
1178
173.9
19.38
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
9.055 ± 0.528
1.487 ± 0.184
6.018 ± 0.226
6.299 ± 0.27
4.087 ± 0.178
7.268 ± 0.271
1.856 ± 0.234
6.262 ± 0.157
6.449 ± 0.295
6.93 ± 0.222
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
3.031 ± 0.167
4.556 ± 0.208
3.525 ± 0.246
4.187 ± 0.313
5.281 ± 0.214
6.83 ± 0.267
5.181 ± 0.261
6.905 ± 0.216
1.381 ± 0.092
3.412 ± 0.169
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here