Streptococcus phage Javan35

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.52

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 60 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4D6ACS0|A0A4D6ACS0_9CAUD Uncharacterized protein OS=Streptococcus phage Javan35 OX=2548120 GN=Javan35_0034 PE=4 SV=1
MM1 pKa = 7.89EE2 pKa = 5.85LLQYY6 pKa = 10.68NNKK9 pKa = 9.88KK10 pKa = 10.05ISLVDD15 pKa = 3.22IDD17 pKa = 4.66DD18 pKa = 5.14EE19 pKa = 4.43IWTGTAYY26 pKa = 11.08YY27 pKa = 10.52CDD29 pKa = 4.3ADD31 pKa = 4.11TNEE34 pKa = 4.15TPEE37 pKa = 4.28DD38 pKa = 3.52VLVVKK43 pKa = 10.12NEE45 pKa = 3.8RR46 pKa = 11.84GYY48 pKa = 9.13TEE50 pKa = 3.79ISEE53 pKa = 4.55SEE55 pKa = 3.94IKK57 pKa = 10.61SIEE60 pKa = 4.11III62 pKa = 3.76

Molecular weight: 7.16 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4D6A8Q5|A0A4D6A8Q5_9CAUD Single-stranded DNA-binding protein OS=Streptococcus phage Javan35 OX=2548120 GN=Javan35_0040 PE=3 SV=1
MM1 pKa = 7.36TKK3 pKa = 7.85QHH5 pKa = 6.91RR6 pKa = 11.84EE7 pKa = 3.47TLIWYY12 pKa = 7.66RR13 pKa = 11.84ASHH16 pKa = 5.37QEE18 pKa = 4.01RR19 pKa = 11.84EE20 pKa = 4.04KK21 pKa = 11.28LLDD24 pKa = 3.94FGLVDD29 pKa = 3.68KK30 pKa = 10.91SQYY33 pKa = 8.62VTLLRR38 pKa = 11.84QLRR41 pKa = 11.84KK42 pKa = 9.91KK43 pKa = 10.48YY44 pKa = 10.73AII46 pKa = 4.1

Molecular weight: 5.69 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

60

0

60

11139

43

1374

185.7

21.06

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.293 ± 0.37

0.682 ± 0.103

6.383 ± 0.275

7.047 ± 0.458

3.959 ± 0.229

6.266 ± 0.504

1.517 ± 0.178

7.487 ± 0.267

8.879 ± 0.443

8.223 ± 0.334

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.406 ± 0.207

6.078 ± 0.264

2.801 ± 0.172

3.86 ± 0.224

4.022 ± 0.221

6.545 ± 0.298

6.096 ± 0.299

5.916 ± 0.171

1.302 ± 0.11

4.237 ± 0.248
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski