Bacteroidales bacterium CF

Taxonomy: cellular organisms; Bacteria; FCB group; Bacteroidetes/Chlorobi group; Bacteroidetes; Bacteroidia; Bacteroidales; unclassified Bacteroidales

Average proteome isoelectric point is 6.63

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2289 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|U5Q811|U5Q811_9BACT Uncharacterized protein OS=Bacteroidales bacterium CF OX=1400053 GN=BRDCF_p1961 PE=4 SV=1
MM1 pKa = 7.58KK2 pKa = 10.15FYY4 pKa = 11.18NLDD7 pKa = 3.48TEE9 pKa = 4.63FTFGKK14 pKa = 10.77YY15 pKa = 8.57EE16 pKa = 4.1GKK18 pKa = 8.02TVRR21 pKa = 11.84EE22 pKa = 3.88ILEE25 pKa = 4.01IQPAYY30 pKa = 10.85LDD32 pKa = 3.31WCAINLDD39 pKa = 3.51HH40 pKa = 7.24FYY42 pKa = 10.59ISEE45 pKa = 4.07EE46 pKa = 4.0VIEE49 pKa = 4.63EE50 pKa = 4.13IKK52 pKa = 11.05VSNSDD57 pKa = 3.49FKK59 pKa = 11.06ISEE62 pKa = 4.06EE63 pKa = 4.25GVNKK67 pKa = 10.43LNEE70 pKa = 4.5KK71 pKa = 8.93YY72 pKa = 9.75EE73 pKa = 4.0QWEE76 pKa = 4.81GEE78 pKa = 4.04QEE80 pKa = 4.68DD81 pKa = 4.18YY82 pKa = 11.52DD83 pKa = 4.51EE84 pKa = 5.37EE85 pKa = 4.8YY86 pKa = 11.38NDD88 pKa = 3.42YY89 pKa = 11.43DD90 pKa = 4.31DD91 pKa = 4.29YY92 pKa = 11.98NEE94 pKa = 4.12RR95 pKa = 11.84EE96 pKa = 4.35SYY98 pKa = 10.62GKK100 pKa = 10.5YY101 pKa = 9.83AGSYY105 pKa = 8.12AQDD108 pKa = 3.52VEE110 pKa = 4.38GLSDD114 pKa = 4.95DD115 pKa = 5.56FIDD118 pKa = 5.28DD119 pKa = 4.07VLDD122 pKa = 4.22GEE124 pKa = 5.0PDD126 pKa = 3.51AYY128 pKa = 10.1WNIDD132 pKa = 2.95

Molecular weight: 15.71 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|U5Q7T9|U5Q7T9_9BACT 50S ribosomal protein L28 OS=Bacteroidales bacterium CF OX=1400053 GN=rpmB PE=3 SV=1
MM1 pKa = 8.15AIRR4 pKa = 11.84KK5 pKa = 7.55FKK7 pKa = 10.23PATPGTRR14 pKa = 11.84HH15 pKa = 5.73KK16 pKa = 10.59AISAFTEE23 pKa = 4.48ITCTTPEE30 pKa = 4.1KK31 pKa = 10.93SLLEE35 pKa = 4.67PIRR38 pKa = 11.84KK39 pKa = 7.82TGGRR43 pKa = 11.84NAQGKK48 pKa = 5.97MTMRR52 pKa = 11.84YY53 pKa = 9.1IGGGHH58 pKa = 5.7KK59 pKa = 10.21RR60 pKa = 11.84MYY62 pKa = 10.29RR63 pKa = 11.84VIDD66 pKa = 3.63FLRR69 pKa = 11.84DD70 pKa = 3.16KK71 pKa = 11.14DD72 pKa = 4.0NYY74 pKa = 7.98TATVKK79 pKa = 10.13TIEE82 pKa = 4.02YY83 pKa = 10.02DD84 pKa = 3.43PNRR87 pKa = 11.84SARR90 pKa = 11.84IALVVYY96 pKa = 10.66ADD98 pKa = 3.39GEE100 pKa = 4.1KK101 pKa = 10.31RR102 pKa = 11.84YY103 pKa = 10.18IIAPNGLKK111 pKa = 10.17VGQVIASGSGIAPEE125 pKa = 4.93LGNTLPLSEE134 pKa = 5.05IPLGTLIHH142 pKa = 6.51NIEE145 pKa = 4.24LRR147 pKa = 11.84PGQGAAMARR156 pKa = 11.84SAGTYY161 pKa = 9.1AQLTARR167 pKa = 11.84EE168 pKa = 4.24GNHH171 pKa = 7.25AILKK175 pKa = 9.5LPSGEE180 pKa = 3.81TRR182 pKa = 11.84MVLVACRR189 pKa = 11.84ATVGTVSNPDD199 pKa = 3.39HH200 pKa = 6.66NLEE203 pKa = 4.25SDD205 pKa = 3.74GKK207 pKa = 10.17AGRR210 pKa = 11.84KK211 pKa = 8.34RR212 pKa = 11.84WLGRR216 pKa = 11.84RR217 pKa = 11.84PRR219 pKa = 11.84NRR221 pKa = 11.84GVVMNPVDD229 pKa = 3.54HH230 pKa = 7.05PMGGGEE236 pKa = 3.89GRR238 pKa = 11.84ASGGHH243 pKa = 5.06PRR245 pKa = 11.84SRR247 pKa = 11.84KK248 pKa = 8.98GLLAKK253 pKa = 10.22GYY255 pKa = 7.57KK256 pKa = 8.18TRR258 pKa = 11.84NPKK261 pKa = 8.55KK262 pKa = 6.78TTKK265 pKa = 10.46KK266 pKa = 10.46FIIEE270 pKa = 4.04RR271 pKa = 11.84RR272 pKa = 11.84KK273 pKa = 10.07KK274 pKa = 10.15

Molecular weight: 29.94 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2289

0

2289

808366

37

1740

353.2

39.7

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.704 ± 0.046

1.044 ± 0.018

5.369 ± 0.03

6.548 ± 0.05

4.747 ± 0.033

6.826 ± 0.048

1.56 ± 0.018

7.985 ± 0.042

7.246 ± 0.046

9.133 ± 0.051

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.617 ± 0.024

5.188 ± 0.037

3.622 ± 0.024

2.894 ± 0.024

4.411 ± 0.03

7.119 ± 0.045

5.315 ± 0.035

6.406 ± 0.037

1.024 ± 0.016

4.24 ± 0.035
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski