Amino acid dipepetide frequency for Sphingomonas sp. Leaf20

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	20.483AlaAla: 20.483 ± 0.197	1.05AlaCys: 1.05 ± 0.032	8.255AlaAsp: 8.255 ± 0.1	6.786AlaGlu: 6.786 ± 0.086	4.492AlaPhe: 4.492 ± 0.07	12.229AlaGly: 12.229 ± 0.119	2.32AlaHis: 2.32 ± 0.046	7.511AlaIle: 7.511 ± 0.078	4.051AlaLys: 4.051 ± 0.07	13.859AlaLeu: 13.859 ± 0.126	4.001AlaMet: 4.001 ± 0.069	3.35AlaAsn: 3.35 ± 0.07	6.676AlaPro: 6.676 ± 0.108	4.614AlaGln: 4.614 ± 0.082	10.195AlaArg: 10.195 ± 0.133	6.92AlaSer: 6.92 ± 0.088	8.152AlaThr: 8.152 ± 0.107	9.59AlaVal: 9.59 ± 0.111	1.642AlaTrp: 1.642 ± 0.045	2.635AlaTyr: 2.635 ± 0.052	0.002AlaXaa: 0.002 ± 0.001
Cys	0.951CysAla: 0.951 ± 0.03	0.081CysCys: 0.081 ± 0.009	0.507CysAsp: 0.507 ± 0.024	0.345CysGlu: 0.345 ± 0.017	0.262CysPhe: 0.262 ± 0.016	0.764CysGly: 0.764 ± 0.029	0.152CysHis: 0.152 ± 0.011	0.327CysIle: 0.327 ± 0.018	0.142CysLys: 0.142 ± 0.012	0.605CysLeu: 0.605 ± 0.022	0.136CysMet: 0.136 ± 0.012	0.187CysAsn: 0.187 ± 0.013	0.382CysPro: 0.382 ± 0.017	0.141CysGln: 0.141 ± 0.011	0.51CysArg: 0.51 ± 0.024	0.373CysSer: 0.373 ± 0.017	0.388CysThr: 0.388 ± 0.018	0.555CysVal: 0.555 ± 0.021	0.101CysTrp: 0.101 ± 0.01	0.16CysTyr: 0.16 ± 0.011	0.0CysXaa: 0.0 ± 0.0
Asp	8.884AspAla: 8.884 ± 0.11	0.443AspCys: 0.443 ± 0.021	3.665AspAsp: 3.665 ± 0.063	3.005AspGlu: 3.005 ± 0.062	2.062AspPhe: 2.062 ± 0.044	6.048AspGly: 6.048 ± 0.092	1.335AspHis: 1.335 ± 0.038	2.863AspIle: 2.863 ± 0.047	1.503AspLys: 1.503 ± 0.043	5.854AspLeu: 5.854 ± 0.077	1.323AspMet: 1.323 ± 0.037	1.178AspAsn: 1.178 ± 0.034	3.949AspPro: 3.949 ± 0.067	1.904AspGln: 1.904 ± 0.04	5.445AspArg: 5.445 ± 0.076	2.328AspSer: 2.328 ± 0.043	3.299AspThr: 3.299 ± 0.048	4.538AspVal: 4.538 ± 0.067	1.098AspTrp: 1.098 ± 0.03	1.545AspTyr: 1.545 ± 0.04	0.0AspXaa: 0.0 ± 0.0
Glu	6.696GluAla: 6.696 ± 0.09	0.256GluCys: 0.256 ± 0.016	2.426GluAsp: 2.426 ± 0.054	2.044GluGlu: 2.044 ± 0.054	1.272GluPhe: 1.272 ± 0.039	3.896GluGly: 3.896 ± 0.062	1.041GluHis: 1.041 ± 0.032	2.605GluIle: 2.605 ± 0.048	1.493GluLys: 1.493 ± 0.041	4.115GluLeu: 4.115 ± 0.062	1.195GluMet: 1.195 ± 0.034	1.093GluAsn: 1.093 ± 0.029	2.306GluPro: 2.306 ± 0.04	1.762GluGln: 1.762 ± 0.044	4.587GluArg: 4.587 ± 0.068	2.019GluSer: 2.019 ± 0.039	3.279GluThr: 3.279 ± 0.054	3.081GluVal: 3.081 ± 0.055	0.633GluTrp: 0.633 ± 0.025	0.844GluTyr: 0.844 ± 0.026	0.0GluXaa: 0.0 ± 0.0
Phe	5.079PheAla: 5.079 ± 0.072	0.282PheCys: 0.282 ± 0.015	2.723PheAsp: 2.723 ± 0.047	1.777PheGlu: 1.777 ± 0.042	1.201PhePhe: 1.201 ± 0.032	3.697PheGly: 3.697 ± 0.06	0.682PheHis: 0.682 ± 0.023	1.279PheIle: 1.279 ± 0.029	0.819PheLys: 0.819 ± 0.03	2.87PheLeu: 2.87 ± 0.054	0.663PheMet: 0.663 ± 0.026	0.93PheAsn: 0.93 ± 0.03	1.37PhePro: 1.37 ± 0.037	0.846PheGln: 0.846 ± 0.028	2.153PheArg: 2.153 ± 0.044	1.85PheSer: 1.85 ± 0.038	2.089PheThr: 2.089 ± 0.039	2.704PheVal: 2.704 ± 0.054	0.492PheTrp: 0.492 ± 0.02	0.841PheTyr: 0.841 ± 0.028	0.0PheXaa: 0.0 ± 0.0
Gly	10.764GlyAla: 10.764 ± 0.129	0.825GlyCys: 0.825 ± 0.027	5.245GlyAsp: 5.245 ± 0.071	4.337GlyGlu: 4.337 ± 0.075	3.677GlyPhe: 3.677 ± 0.058	8.678GlyGly: 8.678 ± 0.141	1.832GlyHis: 1.832 ± 0.039	4.756GlyIle: 4.756 ± 0.071	2.94GlyLys: 2.94 ± 0.065	8.482GlyLeu: 8.482 ± 0.098	2.247GlyMet: 2.247 ± 0.05	2.418GlyAsn: 2.418 ± 0.08	3.516GlyPro: 3.516 ± 0.056	2.931GlyGln: 2.931 ± 0.057	6.616GlyArg: 6.616 ± 0.079	5.014GlySer: 5.014 ± 0.086	5.569GlyThr: 5.569 ± 0.156	6.853GlyVal: 6.853 ± 0.087	1.68GlyTrp: 1.68 ± 0.039	2.497GlyTyr: 2.497 ± 0.05	0.001GlyXaa: 0.001 ± 0.001
His	2.581HisAla: 2.581 ± 0.053	0.186HisCys: 0.186 ± 0.011	1.329HisAsp: 1.329 ± 0.035	0.914HisGlu: 0.914 ± 0.027	0.679HisPhe: 0.679 ± 0.026	1.941HisGly: 1.941 ± 0.045	0.557HisHis: 0.557 ± 0.024	0.825HisIle: 0.825 ± 0.024	0.384HisLys: 0.384 ± 0.021	1.762HisLeu: 1.762 ± 0.042	0.407HisMet: 0.407 ± 0.017	0.389HisAsn: 0.389 ± 0.019	1.22HisPro: 1.22 ± 0.037	0.526HisGln: 0.526 ± 0.02	1.429HisArg: 1.429 ± 0.037	0.745HisSer: 0.745 ± 0.025	0.74HisThr: 0.74 ± 0.026	1.503HisVal: 1.503 ± 0.038	0.32HisTrp: 0.32 ± 0.017	0.509HisTyr: 0.509 ± 0.023	0.0HisXaa: 0.0 ± 0.0
Ile	8.588IleAla: 8.588 ± 0.087	0.348IleCys: 0.348 ± 0.019	4.273IleAsp: 4.273 ± 0.065	3.222IleGlu: 3.222 ± 0.055	1.354IlePhe: 1.354 ± 0.041	5.465IleGly: 5.465 ± 0.075	0.849IleHis: 0.849 ± 0.027	1.796IleIle: 1.796 ± 0.041	1.104IleLys: 1.104 ± 0.033	4.128IleLeu: 4.128 ± 0.066	0.852IleMet: 0.852 ± 0.027	1.255IleAsn: 1.255 ± 0.039	2.225IlePro: 2.225 ± 0.047	1.111IleGln: 1.111 ± 0.033	3.23IleArg: 3.23 ± 0.059	2.262IleSer: 2.262 ± 0.047	2.762IleThr: 2.762 ± 0.062	4.586IleVal: 4.586 ± 0.062	0.535IleTrp: 0.535 ± 0.022	0.868IleTyr: 0.868 ± 0.026	0.002IleXaa: 0.002 ± 0.001
Lys	3.729LysAla: 3.729 ± 0.075	0.13LysCys: 0.13 ± 0.01	1.403LysAsp: 1.403 ± 0.036	0.839LysGlu: 0.839 ± 0.029	0.782LysPhe: 0.782 ± 0.024	2.331LysGly: 2.331 ± 0.056	0.448LysHis: 0.448 ± 0.018	1.343LysIle: 1.343 ± 0.037	0.842LysLys: 0.842 ± 0.035	2.832LysLeu: 2.832 ± 0.057	0.676LysMet: 0.676 ± 0.025	0.613LysAsn: 0.613 ± 0.025	1.968LysPro: 1.968 ± 0.047	0.818LysGln: 0.818 ± 0.028	2.093LysArg: 2.093 ± 0.045	1.421LysSer: 1.421 ± 0.036	1.818LysThr: 1.818 ± 0.043	1.928LysVal: 1.928 ± 0.048	0.31LysTrp: 0.31 ± 0.017	0.55LysTyr: 0.55 ± 0.021	0.001LysXaa: 0.001 ± 0.001
Leu	13.999LeuAla: 13.999 ± 0.129	0.708LeuCys: 0.708 ± 0.027	6.362LeuAsp: 6.362 ± 0.079	4.045LeuGlu: 4.045 ± 0.076	3.411LeuPhe: 3.411 ± 0.065	8.4LeuGly: 8.4 ± 0.099	1.728LeuHis: 1.728 ± 0.039	4.687LeuIle: 4.687 ± 0.07	2.732LeuLys: 2.732 ± 0.051	8.821LeuLeu: 8.821 ± 0.107	2.001LeuMet: 2.001 ± 0.044	2.306LeuAsn: 2.306 ± 0.048	5.346LeuPro: 5.346 ± 0.073	2.419LeuGln: 2.419 ± 0.048	6.648LeuArg: 6.648 ± 0.077	5.711LeuSer: 5.711 ± 0.081	5.886LeuThr: 5.886 ± 0.083	7.49LeuVal: 7.49 ± 0.086	1.187LeuTrp: 1.187 ± 0.032	1.996LeuTyr: 1.996 ± 0.041	0.001LeuXaa: 0.001 ± 0.001
Met	3.186MetAla: 3.186 ± 0.053	0.121MetCys: 0.121 ± 0.01	1.049MetAsp: 1.049 ± 0.032	0.812MetGlu: 0.812 ± 0.025	0.678MetPhe: 0.678 ± 0.025	1.733MetGly: 1.733 ± 0.038	0.407MetHis: 0.407 ± 0.017	1.48MetIle: 1.48 ± 0.033	0.804MetLys: 0.804 ± 0.023	2.705MetLeu: 2.705 ± 0.047	0.634MetMet: 0.634 ± 0.026	0.618MetAsn: 0.618 ± 0.025	1.53MetPro: 1.53 ± 0.038	0.699MetGln: 0.699 ± 0.025	1.76MetArg: 1.76 ± 0.037	1.314MetSer: 1.314 ± 0.037	2.056MetThr: 2.056 ± 0.045	1.629MetVal: 1.629 ± 0.037	0.169MetTrp: 0.169 ± 0.012	0.271MetTyr: 0.271 ± 0.015	0.002MetXaa: 0.002 ± 0.002
Asn	3.405AsnAla: 3.405 ± 0.052	0.208AsnCys: 0.208 ± 0.015	1.464AsnAsp: 1.464 ± 0.042	1.002AsnGlu: 1.002 ± 0.028	0.887AsnPhe: 0.887 ± 0.029	2.471AsnGly: 2.471 ± 0.063	0.451AsnHis: 0.451 ± 0.018	1.216AsnIle: 1.216 ± 0.035	0.557AsnLys: 0.557 ± 0.023	2.318AsnLeu: 2.318 ± 0.058	0.509AsnMet: 0.509 ± 0.022	0.676AsnAsn: 0.676 ± 0.039	1.663AsnPro: 1.663 ± 0.036	0.695AsnGln: 0.695 ± 0.023	1.728AsnArg: 1.728 ± 0.038	1.182AsnSer: 1.182 ± 0.06	1.29AsnThr: 1.29 ± 0.048	1.885AsnVal: 1.885 ± 0.054	0.378AsnTrp: 0.378 ± 0.017	0.676AsnTyr: 0.676 ± 0.033	0.0AsnXaa: 0.0 ± 0.0
Pro	7.457ProAla: 7.457 ± 0.107	0.294ProCys: 0.294 ± 0.016	3.91ProAsp: 3.91 ± 0.063	2.884ProGlu: 2.884 ± 0.052	1.834ProPhe: 1.834 ± 0.037	4.951ProGly: 4.951 ± 0.074	0.956ProHis: 0.956 ± 0.028	2.601ProIle: 2.601 ± 0.042	1.389ProLys: 1.389 ± 0.037	4.739ProLeu: 4.739 ± 0.067	1.217ProMet: 1.217 ± 0.029	1.249ProAsn: 1.249 ± 0.033	2.592ProPro: 2.592 ± 0.066	1.557ProGln: 1.557 ± 0.04	3.207ProArg: 3.207 ± 0.067	2.781ProSer: 2.781 ± 0.05	2.926ProThr: 2.926 ± 0.051	4.498ProVal: 4.498 ± 0.069	0.669ProTrp: 0.669 ± 0.026	1.116ProTyr: 1.116 ± 0.031	0.001ProXaa: 0.001 ± 0.001
Gln	4.172GlnAla: 4.172 ± 0.067	0.192GlnCys: 0.192 ± 0.012	1.41GlnAsp: 1.41 ± 0.036	0.962GlnGlu: 0.962 ± 0.03	1.034GlnPhe: 1.034 ± 0.028	2.416GlnGly: 2.416 ± 0.045	0.563GlnHis: 0.563 ± 0.022	1.719GlnIle: 1.719 ± 0.043	0.756GlnLys: 0.756 ± 0.028	2.878GlnLeu: 2.878 ± 0.056	0.761GlnMet: 0.761 ± 0.024	0.741GlnAsn: 0.741 ± 0.031	1.824GlnPro: 1.824 ± 0.048	1.064GlnGln: 1.064 ± 0.032	2.463GlnArg: 2.463 ± 0.047	1.656GlnSer: 1.656 ± 0.034	1.896GlnThr: 1.896 ± 0.047	2.269GlnVal: 2.269 ± 0.046	0.359GlnTrp: 0.359 ± 0.017	0.629GlnTyr: 0.629 ± 0.024	0.001GlnXaa: 0.001 ± 0.001
Arg	9.245ArgAla: 9.245 ± 0.111	0.456ArgCys: 0.456 ± 0.021	4.661ArgAsp: 4.661 ± 0.065	3.519ArgGlu: 3.519 ± 0.068	3.099ArgPhe: 3.099 ± 0.056	5.374ArgGly: 5.374 ± 0.082	1.652ArgHis: 1.652 ± 0.039	4.223ArgIle: 4.223 ± 0.063	1.847ArgLys: 1.847 ± 0.049	7.881ArgLeu: 7.881 ± 0.097	2.021ArgMet: 2.021 ± 0.041	1.796ArgAsn: 1.796 ± 0.041	3.613ArgPro: 3.613 ± 0.064	2.415ArgGln: 2.415 ± 0.047	5.784ArgArg: 5.784 ± 0.099	3.792ArgSer: 3.792 ± 0.061	4.179ArgThr: 4.179 ± 0.061	5.203ArgVal: 5.203 ± 0.074	1.232ArgTrp: 1.232 ± 0.034	1.983ArgTyr: 1.983 ± 0.045	0.002ArgXaa: 0.002 ± 0.001
Ser	6.497SerAla: 6.497 ± 0.074	0.345SerCys: 0.345 ± 0.017	3.093SerAsp: 3.093 ± 0.049	2.167SerGlu: 2.167 ± 0.04	2.098SerPhe: 2.098 ± 0.042	5.35SerGly: 5.35 ± 0.131	0.911SerHis: 0.911 ± 0.025	2.702SerIle: 2.702 ± 0.053	1.317SerLys: 1.317 ± 0.034	4.908SerLeu: 4.908 ± 0.059	1.181SerMet: 1.181 ± 0.033	1.436SerAsn: 1.436 ± 0.048	2.825SerPro: 2.825 ± 0.058	1.411SerGln: 1.411 ± 0.037	3.389SerArg: 3.389 ± 0.049	2.794SerSer: 2.794 ± 0.069	2.95SerThr: 2.95 ± 0.055	3.949SerVal: 3.949 ± 0.072	0.711SerTrp: 0.711 ± 0.022	1.396SerTyr: 1.396 ± 0.039	0.002SerXaa: 0.002 ± 0.001
Thr	7.661ThrAla: 7.661 ± 0.097	0.36ThrCys: 0.36 ± 0.015	3.355ThrAsp: 3.355 ± 0.055	2.301ThrGlu: 2.301 ± 0.043	1.83ThrPhe: 1.83 ± 0.042	6.117ThrGly: 6.117 ± 0.132	0.958ThrHis: 0.958 ± 0.029	3.622ThrIle: 3.622 ± 0.072	1.357ThrLys: 1.357 ± 0.037	6.74ThrLeu: 6.74 ± 0.103	1.388ThrMet: 1.388 ± 0.034	1.472ThrAsn: 1.472 ± 0.049	4.003ThrPro: 4.003 ± 0.064	1.668ThrGln: 1.668 ± 0.042	4.088ThrArg: 4.088 ± 0.069	2.964ThrSer: 2.964 ± 0.067	3.551ThrThr: 3.551 ± 0.059	4.597ThrVal: 4.597 ± 0.085	0.618ThrTrp: 0.618 ± 0.025	1.29ThrTyr: 1.29 ± 0.034	0.002ThrXaa: 0.002 ± 0.002
Val	11.09ValAla: 11.09 ± 0.106	0.487ValCys: 0.487 ± 0.02	4.776ValAsp: 4.776 ± 0.064	4.154ValGlu: 4.154 ± 0.064	2.376ValPhe: 2.376 ± 0.044	5.933ValGly: 5.933 ± 0.083	1.299ValHis: 1.299 ± 0.031	3.775ValIle: 3.775 ± 0.067	1.885ValLys: 1.885 ± 0.044	6.692ValLeu: 6.692 ± 0.085	1.72ValMet: 1.72 ± 0.037	1.951ValAsn: 1.951 ± 0.052	4.048ValPro: 4.048 ± 0.067	1.988ValGln: 1.988 ± 0.045	5.418ValArg: 5.418 ± 0.066	4.264ValSer: 4.264 ± 0.074	5.097ValThr: 5.097 ± 0.086	6.083ValVal: 6.083 ± 0.084	0.918ValTrp: 0.918 ± 0.026	1.42ValTyr: 1.42 ± 0.033	0.002ValXaa: 0.002 ± 0.001
Trp	1.382TrpAla: 1.382 ± 0.038	0.098TrpCys: 0.098 ± 0.009	0.705TrpAsp: 0.705 ± 0.025	0.474TrpGlu: 0.474 ± 0.019	0.512TrpPhe: 0.512 ± 0.023	0.93TrpGly: 0.93 ± 0.026	0.31TrpHis: 0.31 ± 0.017	0.698TrpIle: 0.698 ± 0.023	0.427TrpLys: 0.427 ± 0.019	1.616TrpLeu: 1.616 ± 0.039	0.327TrpMet: 0.327 ± 0.016	0.426TrpAsn: 0.426 ± 0.018	0.707TrpPro: 0.707 ± 0.025	0.52TrpGln: 0.52 ± 0.022	1.306TrpArg: 1.306 ± 0.032	0.902TrpSer: 0.902 ± 0.023	0.927TrpThr: 0.927 ± 0.031	0.804TrpVal: 0.804 ± 0.03	0.246TrpTrp: 0.246 ± 0.015	0.31TrpTyr: 0.31 ± 0.016	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.962TyrAla: 2.962 ± 0.052	0.201TyrCys: 0.201 ± 0.012	1.658TyrAsp: 1.658 ± 0.044	1.056TyrGlu: 1.056 ± 0.031	0.808TyrPhe: 0.808 ± 0.025	2.154TyrGly: 2.154 ± 0.046	0.492TyrHis: 0.492 ± 0.02	0.786TyrIle: 0.786 ± 0.026	0.526TyrLys: 0.526 ± 0.023	2.075TyrLeu: 2.075 ± 0.045	0.397TyrMet: 0.397 ± 0.018	0.577TyrAsn: 0.577 ± 0.027	1.062TyrPro: 1.062 ± 0.033	0.676TyrGln: 0.676 ± 0.024	1.926TyrArg: 1.926 ± 0.043	1.14TyrSer: 1.14 ± 0.034	1.085TyrThr: 1.085 ± 0.031	1.627TyrVal: 1.627 ± 0.039	0.329TyrTrp: 0.329 ± 0.017	0.651TyrTyr: 0.651 ± 0.026	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.002XaaAla: 0.002 ± 0.002	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.001XaaGly: 0.001 ± 0.001	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.001XaaLys: 0.001 ± 0.001	0.003XaaLeu: 0.003 ± 0.002	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.002XaaPro: 0.002 ± 0.001	0.001XaaGln: 0.001 ± 0.001	0.002XaaArg: 0.002 ± 0.002	0.001XaaSer: 0.001 ± 0.001	0.002XaaThr: 0.002 ± 0.001	0.002XaaVal: 0.002 ± 0.001	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 3757 proteins (1227095 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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