Hubei permutotetra-like virus 5
Average proteome isoelectric point is 7.51
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A1L3KHS8|A0A1L3KHS8_9VIRU C2H2-type domain-containing protein OS=Hubei permutotetra-like virus 5 OX=1923079 PE=4 SV=1
MM1 pKa = 7.6 DD2 pKa = 4.35 TSNPIDD8 pKa = 3.55 NADD11 pKa = 3.67 RR12 pKa = 11.84 VTIGAALKK20 pKa = 10.31 SAEE23 pKa = 4.1 LVRR26 pKa = 11.84 RR27 pKa = 11.84 QDD29 pKa = 3.47 LQFLQEE35 pKa = 3.98 LTATARR41 pKa = 11.84 RR42 pKa = 11.84 TILKK46 pKa = 10.29 VEE48 pKa = 4.53 TKK50 pKa = 10.16 PLDD53 pKa = 3.72 NGEE56 pKa = 4.23 VKK58 pKa = 10.95 LIRR61 pKa = 11.84 SQYY64 pKa = 9.83 LAQLPKK70 pKa = 10.36 VDD72 pKa = 3.78 TTDD75 pKa = 3.36 EE76 pKa = 4.44 PEE78 pKa = 4.34 FPDD81 pKa = 4.34 VLIEE85 pKa = 5.59 KK86 pKa = 9.97 YY87 pKa = 10.33 DD88 pKa = 3.61 QLPVCGIIFEE98 pKa = 4.78 RR99 pKa = 11.84 KK100 pKa = 8.89 PIHH103 pKa = 6.19 PPGRR107 pKa = 11.84 NQLAGSVSTRR117 pKa = 11.84 RR118 pKa = 11.84 VFGGAATAAPLGFQQIAEE136 pKa = 4.22 IAGSNVLQVPSVYY149 pKa = 9.87 TAGTKK154 pKa = 10.09 SGFYY158 pKa = 10.89 NRR160 pKa = 11.84 INKK163 pKa = 8.89 QMGRR167 pKa = 11.84 KK168 pKa = 6.89 TVSIQKK174 pKa = 10.07 AYY176 pKa = 11.09 SRR178 pKa = 11.84 VLGRR182 pKa = 11.84 NVSQRR187 pKa = 11.84 EE188 pKa = 4.0 LVRR191 pKa = 11.84 DD192 pKa = 3.58 LMEE195 pKa = 4.05 IMPRR199 pKa = 11.84 KK200 pKa = 8.47 RR201 pKa = 11.84 TPEE204 pKa = 3.87 TFPDD208 pKa = 3.69 LEE210 pKa = 4.44 EE211 pKa = 5.8 DD212 pKa = 4.46 LDD214 pKa = 3.98 IQLSSLKK221 pKa = 8.92 ITRR224 pKa = 11.84 NASAGAPYY232 pKa = 9.16 WRR234 pKa = 11.84 NKK236 pKa = 9.58 GEE238 pKa = 4.7 CITEE242 pKa = 4.56 IIHH245 pKa = 6.61 GVLPHH250 pKa = 6.49 IVAAIKK256 pKa = 10.33 DD257 pKa = 3.94 GKK259 pKa = 10.7 LEE261 pKa = 3.97 EE262 pKa = 4.53 LFRR265 pKa = 11.84 EE266 pKa = 4.24 QPEE269 pKa = 4.29 LFLVEE274 pKa = 5.34 LKK276 pKa = 11.23 NKK278 pKa = 7.79 MDD280 pKa = 3.85 RR281 pKa = 11.84 YY282 pKa = 10.58 KK283 pKa = 11.01 VGEE286 pKa = 4.59 LNTKK290 pKa = 6.34 TRR292 pKa = 11.84 PYY294 pKa = 10.31 VAVPAHH300 pKa = 5.89 WAFLFSVLSQRR311 pKa = 11.84 FQEE314 pKa = 4.23 TLEE317 pKa = 4.15 VFDD320 pKa = 5.35 GNSKK324 pKa = 10.16 SANAYY329 pKa = 8.95 GFSSAGGGLTRR340 pKa = 11.84 MYY342 pKa = 11.02 KK343 pKa = 9.57 WMRR346 pKa = 11.84 NTKK349 pKa = 9.98 KK350 pKa = 10.68 GEE352 pKa = 3.87 PRR354 pKa = 11.84 FACYY358 pKa = 10.54 GDD360 pKa = 3.89 DD361 pKa = 3.38 TCLVVNHH368 pKa = 7.21 KK369 pKa = 9.27 GTIWRR374 pKa = 11.84 VDD376 pKa = 3.32 PDD378 pKa = 4.07 FQQMDD383 pKa = 3.46 GSIDD387 pKa = 3.73 YY388 pKa = 11.0 DD389 pKa = 3.94 DD390 pKa = 4.46 VKK392 pKa = 10.95 LTIAWIMNTFKK403 pKa = 10.92 QEE405 pKa = 3.85 NPYY408 pKa = 10.68 KK409 pKa = 10.2 DD410 pKa = 3.38 LKK412 pKa = 10.45 FWEE415 pKa = 5.22 VVCNMWLDD423 pKa = 3.83 MAVDD427 pKa = 4.13 PLMILQGPTIYY438 pKa = 10.61 VKK440 pKa = 10.21 RR441 pKa = 11.84 YY442 pKa = 9.81 KK443 pKa = 10.54 SGLLTGIPGTTLFDD457 pKa = 3.76 TVKK460 pKa = 10.51 SVLMYY465 pKa = 10.67 RR466 pKa = 11.84 LYY468 pKa = 9.77 TLEE471 pKa = 4.15 YY472 pKa = 10.36 ASDD475 pKa = 3.88 PQRR478 pKa = 11.84 MLDD481 pKa = 3.92 ASNATNFFQKK491 pKa = 10.26 YY492 pKa = 9.61 GLVIKK497 pKa = 10.36 PGTWSPAAIPMTPEE511 pKa = 4.04 EE512 pKa = 4.67 DD513 pKa = 4.81 CLITDD518 pKa = 4.01 HH519 pKa = 6.99 KK520 pKa = 10.97 FLGVQMKK527 pKa = 9.54 FATYY531 pKa = 8.43 RR532 pKa = 11.84 DD533 pKa = 3.63 KK534 pKa = 11.33 FIVVPTIPTEE544 pKa = 3.79 EE545 pKa = 5.03 AINLMVIQKK554 pKa = 10.35 DD555 pKa = 3.59 DD556 pKa = 3.72 PFNFKK561 pKa = 10.59 NSATSNSRR569 pKa = 11.84 LLFDD573 pKa = 4.49 RR574 pKa = 11.84 MRR576 pKa = 11.84 GNYY579 pKa = 7.62 ITFGFSDD586 pKa = 5.53 PIIQDD591 pKa = 3.57 IIHH594 pKa = 6.19 NVVNSIDD601 pKa = 3.44 PVAIVMRR608 pKa = 11.84 TQVEE612 pKa = 4.42 TGQKK616 pKa = 9.22 PEE618 pKa = 4.08 HH619 pKa = 6.91 LIMSDD624 pKa = 3.08 YY625 pKa = 10.9 SYY627 pKa = 10.56 PDD629 pKa = 2.96 SSGFPSRR636 pKa = 11.84 DD637 pKa = 3.2 FCLSLYY643 pKa = 10.75 AGEE646 pKa = 4.44 EE647 pKa = 4.23 EE648 pKa = 4.1 RR649 pKa = 11.84 AHH651 pKa = 6.98 WIAIYY656 pKa = 10.1 PEE658 pKa = 4.36 LVEE661 pKa = 5.9 IITDD665 pKa = 3.44 LHH667 pKa = 5.15 KK668 pKa = 9.72 TGRR671 pKa = 11.84 NSKK674 pKa = 10.21 LRR676 pKa = 11.84 IVKK679 pKa = 9.57 QDD681 pKa = 3.05 PNMRR685 pKa = 11.84 IEE687 pKa = 4.17 EE688 pKa = 3.92 QLEE691 pKa = 4.19 PEE693 pKa = 3.92 EE694 pKa = 4.26 SLPHH698 pKa = 7.13 KK699 pKa = 9.79 EE700 pKa = 3.99 FAVVAVLPNVTGTIPKK716 pKa = 10.12 KK717 pKa = 9.76 VDD719 pKa = 3.14 PNKK722 pKa = 10.05 RR723 pKa = 11.84 SRR725 pKa = 11.84 IVDD728 pKa = 3.2 EE729 pKa = 4.8 HH730 pKa = 5.88 GQPGKK735 pKa = 10.49 YY736 pKa = 10.29 LPDD739 pKa = 4.02 LAQTIVYY746 pKa = 8.2 EE747 pKa = 4.12 LKK749 pKa = 10.46 KK750 pKa = 10.6 RR751 pKa = 11.84 GIVSIKK757 pKa = 10.19 DD758 pKa = 3.34 LCFHH762 pKa = 7.0 LNLDD766 pKa = 3.73 RR767 pKa = 11.84 EE768 pKa = 4.46 QIRR771 pKa = 11.84 RR772 pKa = 11.84 NNRR775 pKa = 11.84 GFYY778 pKa = 7.61 TTGFSDD784 pKa = 3.76 EE785 pKa = 5.0 DD786 pKa = 3.9 LASLHH791 pKa = 6.65 PIEE794 pKa = 4.37 TAIATSQEE802 pKa = 3.97 AVLEE806 pKa = 4.19 QCTQQAQIIKK816 pKa = 9.14 TSNFGEE822 pKa = 3.9 NRR824 pKa = 11.84 LRR826 pKa = 11.84 QLQEE830 pKa = 3.94 GKK832 pKa = 9.98 QEE834 pKa = 3.82 LSAVVEE840 pKa = 4.2 TAPSKK845 pKa = 11.02 VYY847 pKa = 10.28 IDD849 pKa = 3.27 TMYY852 pKa = 8.98 FTSIPGEE859 pKa = 4.07 PKK861 pKa = 9.9 PGKK864 pKa = 9.72 DD865 pKa = 3.09 QNEE868 pKa = 4.95 CIRR871 pKa = 11.84 NMILWMSAKK880 pKa = 10.12 CRR882 pKa = 11.84 VTSRR886 pKa = 11.84 WRR888 pKa = 11.84 THH890 pKa = 5.43 MSPMNDD896 pKa = 2.48 LFVFLEE902 pKa = 4.3 VMHH905 pKa = 6.13 QGRR908 pKa = 11.84 ARR910 pKa = 11.84 VVASCRR916 pKa = 11.84 CLNQKK921 pKa = 9.64 LGKK924 pKa = 10.32 AYY926 pKa = 8.24 ITRR929 pKa = 11.84 IVYY932 pKa = 8.65 VLKK935 pKa = 10.59 DD936 pKa = 3.59 VPVPSDD942 pKa = 3.1 NFTASSADD950 pKa = 3.44 GTSWAAQVEE959 pKa = 4.59 AEE961 pKa = 4.47 KK962 pKa = 10.82 RR963 pKa = 11.84 PLPEE967 pKa = 4.75 IVDD970 pKa = 4.1 YY971 pKa = 10.48 VWKK974 pKa = 9.25 RR975 pKa = 11.84 TEE977 pKa = 4.4 DD978 pKa = 3.35 YY979 pKa = 10.74 KK980 pKa = 11.37 KK981 pKa = 10.39 QIKK984 pKa = 8.88 TGLEE988 pKa = 3.76 RR989 pKa = 11.84 MDD991 pKa = 3.86 LVIRR995 pKa = 11.84 NPSVATTIGKK1005 pKa = 9.28 GAAKK1009 pKa = 10.0 AALGTVEE1016 pKa = 5.27 SYY1018 pKa = 11.35 PDD1020 pKa = 3.04 VDD1022 pKa = 4.29 FFKK1025 pKa = 10.96 DD1026 pKa = 4.55 FYY1028 pKa = 11.63 KK1029 pKa = 11.01 SMPTSNRR1036 pKa = 11.84 VPKK1039 pKa = 10.08 KK1040 pKa = 10.52 APPHH1044 pKa = 5.94 HH1045 pKa = 6.47 FF1046 pKa = 3.79
Molecular weight: 118.66 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 7.028
IPC2_protein 7.512
IPC_protein 7.351
Toseland 7.132
ProMoST 7.922
Dawson 8.097
Bjellqvist 8.39
Wikipedia 8.039
Rodwell 8.141
Grimsley 7.205
Solomon 8.156
Lehninger 8.156
Nozaki 8.536
DTASelect 8.185
Thurlkill 8.244
EMBOSS 8.317
Sillero 8.536
Patrickios 4.507
IPC_peptide 8.141
IPC2_peptide 7.263
IPC2.peptide.svr19 7.553
Protein with the highest isoelectric point:
>tr|A0A1L3KHS8|A0A1L3KHS8_9VIRU C2H2-type domain-containing protein OS=Hubei permutotetra-like virus 5 OX=1923079 PE=4 SV=1
MM1 pKa = 7.5 KK2 pKa = 10.66 CNVCDD7 pKa = 4.03 RR8 pKa = 11.84 NFKK11 pKa = 9.16 TVQDD15 pKa = 4.33 CAKK18 pKa = 8.28 HH19 pKa = 4.68 TAMVHH24 pKa = 4.52 PTKK27 pKa = 10.79 KK28 pKa = 9.84 PGKK31 pKa = 4.28 TTKK34 pKa = 9.41 RR35 pKa = 11.84 TNRR38 pKa = 11.84 RR39 pKa = 11.84 NRR41 pKa = 11.84 NRR43 pKa = 11.84 RR44 pKa = 11.84 LGAVNNQLGKK54 pKa = 8.09 QTSVGHH60 pKa = 6.2 DD61 pKa = 4.23 LIGIYY66 pKa = 9.07 PVKK69 pKa = 10.66 KK70 pKa = 10.2 DD71 pKa = 3.84 MKK73 pKa = 9.55 PGTIVAYY80 pKa = 10.11 HH81 pKa = 5.8 VLSPQHH87 pKa = 5.72 MVPSRR92 pKa = 11.84 LNTEE96 pKa = 3.69 AALFSRR102 pKa = 11.84 WKK104 pKa = 9.77 PLSLKK109 pKa = 9.32 VTVTNSGAFTTFGALTVAWCPDD131 pKa = 3.11 PTISHH136 pKa = 6.74 RR137 pKa = 11.84 AEE139 pKa = 3.87 NSVQLARR146 pKa = 11.84 ISSFRR151 pKa = 11.84 PSKK154 pKa = 10.39 VVRR157 pKa = 11.84 LNNSVVLNIPTEE169 pKa = 4.32 MPTKK173 pKa = 9.9 WYY175 pKa = 9.52 HH176 pKa = 6.55 CDD178 pKa = 4.25 GIPEE182 pKa = 4.31 LTSHH186 pKa = 6.12 GTLVIMVSAQTGGFDD201 pKa = 3.75 GFLSSTVHH209 pKa = 6.44 LEE211 pKa = 3.39 WRR213 pKa = 11.84 VQFEE217 pKa = 4.8 GIEE220 pKa = 4.04 MPAVIDD226 pKa = 3.38 AAEE229 pKa = 4.31 ILRR232 pKa = 11.84 PDD234 pKa = 4.53 PGWNPPFFTTSDD246 pKa = 3.03 GGYY249 pKa = 10.27 DD250 pKa = 3.68 GSRR253 pKa = 11.84 LILKK257 pKa = 7.09 EE258 pKa = 3.97 HH259 pKa = 7.25 PGGDD263 pKa = 3.15 MVFFSAGRR271 pKa = 11.84 YY272 pKa = 5.94 DD273 pKa = 4.37 TIYY276 pKa = 10.93 GPADD280 pKa = 3.84 GEE282 pKa = 4.28 PPIYY286 pKa = 10.72 AHH288 pKa = 7.05 LEE290 pKa = 4.17 NGSSTEE296 pKa = 4.02 VKK298 pKa = 10.41 HH299 pKa = 6.29 FAIVQNYY306 pKa = 4.47 VHH308 pKa = 7.14 KK309 pKa = 10.92 GLACFNSLEE318 pKa = 4.18 EE319 pKa = 4.02 ARR321 pKa = 11.84 SYY323 pKa = 11.71 VLTGSPDD330 pKa = 3.13 KK331 pKa = 10.85 IMRR334 pKa = 11.84 YY335 pKa = 9.25 IKK337 pKa = 10.73 AGDD340 pKa = 3.51 YY341 pKa = 11.44 SNISKK346 pKa = 10.17 FKK348 pKa = 10.19 IIEE351 pKa = 4.11 VPKK354 pKa = 10.76 SVTQIQAEE362 pKa = 4.11 IDD364 pKa = 3.35 KK365 pKa = 10.23 LQQQIEE371 pKa = 4.34 DD372 pKa = 4.07 QVFLDD377 pKa = 5.04 CPALL381 pKa = 3.88
Molecular weight: 42.33 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 7.983
IPC2_protein 8.097
IPC_protein 7.98
Toseland 8.097
ProMoST 8.521
Dawson 8.785
Bjellqvist 8.931
Wikipedia 8.785
Rodwell 8.858
Grimsley 8.039
Solomon 8.902
Lehninger 8.887
Nozaki 9.121
DTASelect 8.741
Thurlkill 8.829
EMBOSS 8.975
Sillero 9.077
Patrickios 4.571
IPC_peptide 8.902
IPC2_peptide 7.746
IPC2.peptide.svr19 7.786
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2
0
2
1427
381
1046
713.5
80.49
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.377 ± 0.04
1.402 ± 0.222
5.746 ± 0.388
5.746 ± 0.655
4.205 ± 0.003
5.886 ± 0.612
2.172 ± 0.633
6.377 ± 0.174
6.657 ± 0.183
7.638 ± 0.416
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.663 ± 0.153
4.345 ± 0.194
6.167 ± 0.202
4.205 ± 0.271
5.746 ± 0.388
6.097 ± 0.505
6.657 ± 0.219
7.358 ± 0.263
1.261 ± 0.026
3.294 ± 0.207
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here