Hubei permutotetra-like virus 5

Taxonomy: Viruses; Riboviria; unclassified Riboviria; unclassified RNA viruses ShiM-2016

Average proteome isoelectric point is 7.51

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    

Protein with the lowest isoelectric point:
>tr|A0A1L3KHS8|A0A1L3KHS8_9VIRU C2H2-type domain-containing protein OS=Hubei permutotetra-like virus 5 OX=1923079 PE=4 SV=1
MM1 pKa = 7.6DD2 pKa = 4.35TSNPIDD8 pKa = 3.55NADD11 pKa = 3.67RR12 pKa = 11.84VTIGAALKK20 pKa = 10.31SAEE23 pKa = 4.1LVRR26 pKa = 11.84RR27 pKa = 11.84QDD29 pKa = 3.47LQFLQEE35 pKa = 3.98LTATARR41 pKa = 11.84RR42 pKa = 11.84TILKK46 pKa = 10.29VEE48 pKa = 4.53TKK50 pKa = 10.16PLDD53 pKa = 3.72NGEE56 pKa = 4.23VKK58 pKa = 10.95LIRR61 pKa = 11.84SQYY64 pKa = 9.83LAQLPKK70 pKa = 10.36VDD72 pKa = 3.78TTDD75 pKa = 3.36EE76 pKa = 4.44PEE78 pKa = 4.34FPDD81 pKa = 4.34VLIEE85 pKa = 5.59KK86 pKa = 9.97YY87 pKa = 10.33DD88 pKa = 3.61QLPVCGIIFEE98 pKa = 4.78RR99 pKa = 11.84KK100 pKa = 8.89PIHH103 pKa = 6.19PPGRR107 pKa = 11.84NQLAGSVSTRR117 pKa = 11.84RR118 pKa = 11.84VFGGAATAAPLGFQQIAEE136 pKa = 4.22IAGSNVLQVPSVYY149 pKa = 9.87TAGTKK154 pKa = 10.09SGFYY158 pKa = 10.89NRR160 pKa = 11.84INKK163 pKa = 8.89QMGRR167 pKa = 11.84KK168 pKa = 6.89TVSIQKK174 pKa = 10.07AYY176 pKa = 11.09SRR178 pKa = 11.84VLGRR182 pKa = 11.84NVSQRR187 pKa = 11.84EE188 pKa = 4.0LVRR191 pKa = 11.84DD192 pKa = 3.58LMEE195 pKa = 4.05IMPRR199 pKa = 11.84KK200 pKa = 8.47RR201 pKa = 11.84TPEE204 pKa = 3.87TFPDD208 pKa = 3.69LEE210 pKa = 4.44EE211 pKa = 5.8DD212 pKa = 4.46LDD214 pKa = 3.98IQLSSLKK221 pKa = 8.92ITRR224 pKa = 11.84NASAGAPYY232 pKa = 9.16WRR234 pKa = 11.84NKK236 pKa = 9.58GEE238 pKa = 4.7CITEE242 pKa = 4.56IIHH245 pKa = 6.61GVLPHH250 pKa = 6.49IVAAIKK256 pKa = 10.33DD257 pKa = 3.94GKK259 pKa = 10.7LEE261 pKa = 3.97EE262 pKa = 4.53LFRR265 pKa = 11.84EE266 pKa = 4.24QPEE269 pKa = 4.29LFLVEE274 pKa = 5.34LKK276 pKa = 11.23NKK278 pKa = 7.79MDD280 pKa = 3.85RR281 pKa = 11.84YY282 pKa = 10.58KK283 pKa = 11.01VGEE286 pKa = 4.59LNTKK290 pKa = 6.34TRR292 pKa = 11.84PYY294 pKa = 10.31VAVPAHH300 pKa = 5.89WAFLFSVLSQRR311 pKa = 11.84FQEE314 pKa = 4.23TLEE317 pKa = 4.15VFDD320 pKa = 5.35GNSKK324 pKa = 10.16SANAYY329 pKa = 8.95GFSSAGGGLTRR340 pKa = 11.84MYY342 pKa = 11.02KK343 pKa = 9.57WMRR346 pKa = 11.84NTKK349 pKa = 9.98KK350 pKa = 10.68GEE352 pKa = 3.87PRR354 pKa = 11.84FACYY358 pKa = 10.54GDD360 pKa = 3.89DD361 pKa = 3.38TCLVVNHH368 pKa = 7.21KK369 pKa = 9.27GTIWRR374 pKa = 11.84VDD376 pKa = 3.32PDD378 pKa = 4.07FQQMDD383 pKa = 3.46GSIDD387 pKa = 3.73YY388 pKa = 11.0DD389 pKa = 3.94DD390 pKa = 4.46VKK392 pKa = 10.95LTIAWIMNTFKK403 pKa = 10.92QEE405 pKa = 3.85NPYY408 pKa = 10.68KK409 pKa = 10.2DD410 pKa = 3.38LKK412 pKa = 10.45FWEE415 pKa = 5.22VVCNMWLDD423 pKa = 3.83MAVDD427 pKa = 4.13PLMILQGPTIYY438 pKa = 10.61VKK440 pKa = 10.21RR441 pKa = 11.84YY442 pKa = 9.81KK443 pKa = 10.54SGLLTGIPGTTLFDD457 pKa = 3.76TVKK460 pKa = 10.51SVLMYY465 pKa = 10.67RR466 pKa = 11.84LYY468 pKa = 9.77TLEE471 pKa = 4.15YY472 pKa = 10.36ASDD475 pKa = 3.88PQRR478 pKa = 11.84MLDD481 pKa = 3.92ASNATNFFQKK491 pKa = 10.26YY492 pKa = 9.61GLVIKK497 pKa = 10.36PGTWSPAAIPMTPEE511 pKa = 4.04EE512 pKa = 4.67DD513 pKa = 4.81CLITDD518 pKa = 4.01HH519 pKa = 6.99KK520 pKa = 10.97FLGVQMKK527 pKa = 9.54FATYY531 pKa = 8.43RR532 pKa = 11.84DD533 pKa = 3.63KK534 pKa = 11.33FIVVPTIPTEE544 pKa = 3.79EE545 pKa = 5.03AINLMVIQKK554 pKa = 10.35DD555 pKa = 3.59DD556 pKa = 3.72PFNFKK561 pKa = 10.59NSATSNSRR569 pKa = 11.84LLFDD573 pKa = 4.49RR574 pKa = 11.84MRR576 pKa = 11.84GNYY579 pKa = 7.62ITFGFSDD586 pKa = 5.53PIIQDD591 pKa = 3.57IIHH594 pKa = 6.19NVVNSIDD601 pKa = 3.44PVAIVMRR608 pKa = 11.84TQVEE612 pKa = 4.42TGQKK616 pKa = 9.22PEE618 pKa = 4.08HH619 pKa = 6.91LIMSDD624 pKa = 3.08YY625 pKa = 10.9SYY627 pKa = 10.56PDD629 pKa = 2.96SSGFPSRR636 pKa = 11.84DD637 pKa = 3.2FCLSLYY643 pKa = 10.75AGEE646 pKa = 4.44EE647 pKa = 4.23EE648 pKa = 4.1RR649 pKa = 11.84AHH651 pKa = 6.98WIAIYY656 pKa = 10.1PEE658 pKa = 4.36LVEE661 pKa = 5.9IITDD665 pKa = 3.44LHH667 pKa = 5.15KK668 pKa = 9.72TGRR671 pKa = 11.84NSKK674 pKa = 10.21LRR676 pKa = 11.84IVKK679 pKa = 9.57QDD681 pKa = 3.05PNMRR685 pKa = 11.84IEE687 pKa = 4.17EE688 pKa = 3.92QLEE691 pKa = 4.19PEE693 pKa = 3.92EE694 pKa = 4.26SLPHH698 pKa = 7.13KK699 pKa = 9.79EE700 pKa = 3.99FAVVAVLPNVTGTIPKK716 pKa = 10.12KK717 pKa = 9.76VDD719 pKa = 3.14PNKK722 pKa = 10.05RR723 pKa = 11.84SRR725 pKa = 11.84IVDD728 pKa = 3.2EE729 pKa = 4.8HH730 pKa = 5.88GQPGKK735 pKa = 10.49YY736 pKa = 10.29LPDD739 pKa = 4.02LAQTIVYY746 pKa = 8.2EE747 pKa = 4.12LKK749 pKa = 10.46KK750 pKa = 10.6RR751 pKa = 11.84GIVSIKK757 pKa = 10.19DD758 pKa = 3.34LCFHH762 pKa = 7.0LNLDD766 pKa = 3.73RR767 pKa = 11.84EE768 pKa = 4.46QIRR771 pKa = 11.84RR772 pKa = 11.84NNRR775 pKa = 11.84GFYY778 pKa = 7.61TTGFSDD784 pKa = 3.76EE785 pKa = 5.0DD786 pKa = 3.9LASLHH791 pKa = 6.65PIEE794 pKa = 4.37TAIATSQEE802 pKa = 3.97AVLEE806 pKa = 4.19QCTQQAQIIKK816 pKa = 9.14TSNFGEE822 pKa = 3.9NRR824 pKa = 11.84LRR826 pKa = 11.84QLQEE830 pKa = 3.94GKK832 pKa = 9.98QEE834 pKa = 3.82LSAVVEE840 pKa = 4.2TAPSKK845 pKa = 11.02VYY847 pKa = 10.28IDD849 pKa = 3.27TMYY852 pKa = 8.98FTSIPGEE859 pKa = 4.07PKK861 pKa = 9.9PGKK864 pKa = 9.72DD865 pKa = 3.09QNEE868 pKa = 4.95CIRR871 pKa = 11.84NMILWMSAKK880 pKa = 10.12CRR882 pKa = 11.84VTSRR886 pKa = 11.84WRR888 pKa = 11.84THH890 pKa = 5.43MSPMNDD896 pKa = 2.48LFVFLEE902 pKa = 4.3VMHH905 pKa = 6.13QGRR908 pKa = 11.84ARR910 pKa = 11.84VVASCRR916 pKa = 11.84CLNQKK921 pKa = 9.64LGKK924 pKa = 10.32AYY926 pKa = 8.24ITRR929 pKa = 11.84IVYY932 pKa = 8.65VLKK935 pKa = 10.59DD936 pKa = 3.59VPVPSDD942 pKa = 3.1NFTASSADD950 pKa = 3.44GTSWAAQVEE959 pKa = 4.59AEE961 pKa = 4.47KK962 pKa = 10.82RR963 pKa = 11.84PLPEE967 pKa = 4.75IVDD970 pKa = 4.1YY971 pKa = 10.48VWKK974 pKa = 9.25RR975 pKa = 11.84TEE977 pKa = 4.4DD978 pKa = 3.35YY979 pKa = 10.74KK980 pKa = 11.37KK981 pKa = 10.39QIKK984 pKa = 8.88TGLEE988 pKa = 3.76RR989 pKa = 11.84MDD991 pKa = 3.86LVIRR995 pKa = 11.84NPSVATTIGKK1005 pKa = 9.28GAAKK1009 pKa = 10.0AALGTVEE1016 pKa = 5.27SYY1018 pKa = 11.35PDD1020 pKa = 3.04VDD1022 pKa = 4.29FFKK1025 pKa = 10.96DD1026 pKa = 4.55FYY1028 pKa = 11.63KK1029 pKa = 11.01SMPTSNRR1036 pKa = 11.84VPKK1039 pKa = 10.08KK1040 pKa = 10.52APPHH1044 pKa = 5.94HH1045 pKa = 6.47FF1046 pKa = 3.79

Molecular weight:
118.66 kDa
Isoelectric point according different methods:






Protein with the highest isoelectric point:
>tr|A0A1L3KHS8|A0A1L3KHS8_9VIRU C2H2-type domain-containing protein OS=Hubei permutotetra-like virus 5 OX=1923079 PE=4 SV=1
MM1 pKa = 7.5KK2 pKa = 10.66CNVCDD7 pKa = 4.03RR8 pKa = 11.84NFKK11 pKa = 9.16TVQDD15 pKa = 4.33CAKK18 pKa = 8.28HH19 pKa = 4.68TAMVHH24 pKa = 4.52PTKK27 pKa = 10.79KK28 pKa = 9.84PGKK31 pKa = 4.28TTKK34 pKa = 9.41RR35 pKa = 11.84TNRR38 pKa = 11.84RR39 pKa = 11.84NRR41 pKa = 11.84NRR43 pKa = 11.84RR44 pKa = 11.84LGAVNNQLGKK54 pKa = 8.09QTSVGHH60 pKa = 6.2DD61 pKa = 4.23LIGIYY66 pKa = 9.07PVKK69 pKa = 10.66KK70 pKa = 10.2DD71 pKa = 3.84MKK73 pKa = 9.55PGTIVAYY80 pKa = 10.11HH81 pKa = 5.8VLSPQHH87 pKa = 5.72MVPSRR92 pKa = 11.84LNTEE96 pKa = 3.69AALFSRR102 pKa = 11.84WKK104 pKa = 9.77PLSLKK109 pKa = 9.32VTVTNSGAFTTFGALTVAWCPDD131 pKa = 3.11PTISHH136 pKa = 6.74RR137 pKa = 11.84AEE139 pKa = 3.87NSVQLARR146 pKa = 11.84ISSFRR151 pKa = 11.84PSKK154 pKa = 10.39VVRR157 pKa = 11.84LNNSVVLNIPTEE169 pKa = 4.32MPTKK173 pKa = 9.9WYY175 pKa = 9.52HH176 pKa = 6.55CDD178 pKa = 4.25GIPEE182 pKa = 4.31LTSHH186 pKa = 6.12GTLVIMVSAQTGGFDD201 pKa = 3.75GFLSSTVHH209 pKa = 6.44LEE211 pKa = 3.39WRR213 pKa = 11.84VQFEE217 pKa = 4.8GIEE220 pKa = 4.04MPAVIDD226 pKa = 3.38AAEE229 pKa = 4.31ILRR232 pKa = 11.84PDD234 pKa = 4.53PGWNPPFFTTSDD246 pKa = 3.03GGYY249 pKa = 10.27DD250 pKa = 3.68GSRR253 pKa = 11.84LILKK257 pKa = 7.09EE258 pKa = 3.97HH259 pKa = 7.25PGGDD263 pKa = 3.15MVFFSAGRR271 pKa = 11.84YY272 pKa = 5.94DD273 pKa = 4.37TIYY276 pKa = 10.93GPADD280 pKa = 3.84GEE282 pKa = 4.28PPIYY286 pKa = 10.72AHH288 pKa = 7.05LEE290 pKa = 4.17NGSSTEE296 pKa = 4.02VKK298 pKa = 10.41HH299 pKa = 6.29FAIVQNYY306 pKa = 4.47VHH308 pKa = 7.14KK309 pKa = 10.92GLACFNSLEE318 pKa = 4.18EE319 pKa = 4.02ARR321 pKa = 11.84SYY323 pKa = 11.71VLTGSPDD330 pKa = 3.13KK331 pKa = 10.85IMRR334 pKa = 11.84YY335 pKa = 9.25IKK337 pKa = 10.73AGDD340 pKa = 3.51YY341 pKa = 11.44SNISKK346 pKa = 10.17FKK348 pKa = 10.19IIEE351 pKa = 4.11VPKK354 pKa = 10.76SVTQIQAEE362 pKa = 4.11IDD364 pKa = 3.35KK365 pKa = 10.23LQQQIEE371 pKa = 4.34DD372 pKa = 4.07QVFLDD377 pKa = 5.04CPALL381 pKa = 3.88

Molecular weight:
42.33 kDa
Isoelectric point according different methods:






Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2

0

2

1427

381

1046

713.5

80.49

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.377 ± 0.04

1.402 ± 0.222

5.746 ± 0.388

5.746 ± 0.655

4.205 ± 0.003

5.886 ± 0.612

2.172 ± 0.633

6.377 ± 0.174

6.657 ± 0.183

7.638 ± 0.416

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.663 ± 0.153

4.345 ± 0.194

6.167 ± 0.202

4.205 ± 0.271

5.746 ± 0.388

6.097 ± 0.505

6.657 ± 0.219

7.358 ± 0.263

1.261 ± 0.026

3.294 ± 0.207

Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski