Gayfeather mild mottle virus

Taxonomy: Viruses; Riboviria; Orthornavirae; Kitrinoviricota; Alsuviricetes; Martellivirales; Bromoviridae; Cucumovirus

Average proteome isoelectric point is 6.73

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|C0MNB4|C0MNB4_9BROM 2b protein OS=Gayfeather mild mottle virus OX=578305 GN=2b PE=4 SV=1
MM1 pKa = 7.83ASTDD5 pKa = 3.68VIFSLEE11 pKa = 3.77NLLNGSYY18 pKa = 10.96GVVTPEE24 pKa = 3.98DD25 pKa = 3.52DD26 pKa = 3.47ALFRR30 pKa = 11.84QRR32 pKa = 11.84SMYY35 pKa = 9.89DD36 pKa = 2.94ASVSDD41 pKa = 4.86DD42 pKa = 4.81SIITDD47 pKa = 4.35DD48 pKa = 4.38DD49 pKa = 3.98DD50 pKa = 5.68AVAFTCDD57 pKa = 3.41DD58 pKa = 3.41VSADD62 pKa = 3.35GHH64 pKa = 6.14GEE66 pKa = 4.25SNPSDD71 pKa = 3.65PCSEE75 pKa = 5.16DD76 pKa = 3.07GCQFNDD82 pKa = 3.35NPVLYY87 pKa = 10.45DD88 pKa = 3.31IEE90 pKa = 4.78EE91 pKa = 4.2YY92 pKa = 10.42QALSDD97 pKa = 4.13SEE99 pKa = 5.0LFALFDD105 pKa = 4.57TIVKK109 pKa = 9.27PIKK112 pKa = 9.98FGSILTPEE120 pKa = 4.65FSRR123 pKa = 11.84PRR125 pKa = 11.84FYY127 pKa = 10.65TALSLAKK134 pKa = 9.78SVSVISNRR142 pKa = 11.84PSTFGEE148 pKa = 4.2VSPEE152 pKa = 4.16LIKK155 pKa = 10.71AIYY158 pKa = 9.28IGEE161 pKa = 5.04PITSVSGAHH170 pKa = 6.37AVGDD174 pKa = 4.04TPDD177 pKa = 3.66DD178 pKa = 3.74CEE180 pKa = 5.44GYY182 pKa = 10.68VFVPSEE188 pKa = 3.94PTSNFEE194 pKa = 4.05PPPICEE200 pKa = 4.14EE201 pKa = 4.25CGLAAYY207 pKa = 8.06HH208 pKa = 6.64CPHH211 pKa = 7.0FDD213 pKa = 3.48FNALKK218 pKa = 8.62EE219 pKa = 4.26THH221 pKa = 6.49NDD223 pKa = 2.81HH224 pKa = 6.79TISHH228 pKa = 7.32DD229 pKa = 3.88YY230 pKa = 10.63EE231 pKa = 5.6IEE233 pKa = 4.12TLTGVIDD240 pKa = 5.07DD241 pKa = 4.49ATLLMNLGPFLVPIQCEE258 pKa = 3.83YY259 pKa = 11.57SKK261 pKa = 9.93TFEE264 pKa = 4.59SKK266 pKa = 10.52HH267 pKa = 4.5ATKK270 pKa = 10.31PSLARR275 pKa = 11.84PTDD278 pKa = 3.52RR279 pKa = 11.84VDD281 pKa = 3.14VDD283 pKa = 3.75VVQAVCDD290 pKa = 3.92SMLPTHH296 pKa = 6.68VNYY299 pKa = 10.61DD300 pKa = 3.53DD301 pKa = 4.66TYY303 pKa = 9.07HH304 pKa = 5.8QVFVEE309 pKa = 4.29DD310 pKa = 3.31SDD312 pKa = 4.5YY313 pKa = 11.69SVDD316 pKa = 3.29IDD318 pKa = 5.17RR319 pKa = 11.84IRR321 pKa = 11.84LKK323 pKa = 10.79QSDD326 pKa = 4.85LLPKK330 pKa = 10.52VSDD333 pKa = 4.56DD334 pKa = 3.5GHH336 pKa = 5.5MHH338 pKa = 6.63PVLNTGSGHH347 pKa = 6.19KK348 pKa = 10.0RR349 pKa = 11.84VGTQKK354 pKa = 10.21EE355 pKa = 3.95VHH357 pKa = 5.29TAIKK361 pKa = 9.92KK362 pKa = 9.94RR363 pKa = 11.84NADD366 pKa = 3.49VPEE369 pKa = 5.43LGDD372 pKa = 3.84SVNLTRR378 pKa = 11.84LSEE381 pKa = 4.14AVAEE385 pKa = 4.37RR386 pKa = 11.84FISSYY391 pKa = 10.49MNVNALVSSNFINVVGNFHH410 pKa = 7.75AYY412 pKa = 5.51MQKK415 pKa = 8.24WHH417 pKa = 6.91SSLSYY422 pKa = 11.27DD423 pKa = 5.43DD424 pKa = 6.08LPDD427 pKa = 4.0LNAEE431 pKa = 3.83NLQFYY436 pKa = 9.9EE437 pKa = 4.57HH438 pKa = 6.27MVKK441 pKa = 10.41SDD443 pKa = 3.47VKK445 pKa = 10.83PSVTDD450 pKa = 3.2TLNVDD455 pKa = 3.69RR456 pKa = 11.84PVPATITFHH465 pKa = 6.94KK466 pKa = 10.27KK467 pKa = 10.11QITSQFSPLFTALFEE482 pKa = 4.23RR483 pKa = 11.84FQRR486 pKa = 11.84CLRR489 pKa = 11.84SKK491 pKa = 10.77VVLPVGKK498 pKa = 9.93ISSLEE503 pKa = 3.97LEE505 pKa = 4.67DD506 pKa = 4.67FSVIGKK512 pKa = 8.83HH513 pKa = 5.79CLEE516 pKa = 4.76IDD518 pKa = 3.42LSKK521 pKa = 10.66FDD523 pKa = 3.77KK524 pKa = 11.17SQGEE528 pKa = 4.26LHH530 pKa = 7.24LMIQEE535 pKa = 4.23QILNRR540 pKa = 11.84LGCPAHH546 pKa = 6.52VSKK549 pKa = 9.66WWCDD553 pKa = 3.94FHH555 pKa = 7.13RR556 pKa = 11.84QSYY559 pKa = 10.55IKK561 pKa = 10.22DD562 pKa = 3.05KK563 pKa = 10.76RR564 pKa = 11.84AGVGMALSFQRR575 pKa = 11.84RR576 pKa = 11.84TGDD579 pKa = 2.78AFTYY583 pKa = 10.33FGNTLVTMALFCWCYY598 pKa = 10.03DD599 pKa = 3.43TEE601 pKa = 4.39QFDD604 pKa = 4.07RR605 pKa = 11.84MLFSGDD611 pKa = 3.53DD612 pKa = 3.49SLAFSSIAPVGDD624 pKa = 3.37PSKK627 pKa = 10.44FTTLFNMEE635 pKa = 4.51AKK637 pKa = 10.46VMEE640 pKa = 4.39PSVPYY645 pKa = 10.05ICSKK649 pKa = 10.76FLLTDD654 pKa = 3.4EE655 pKa = 5.17FGQTFSVPDD664 pKa = 4.32PLRR667 pKa = 11.84EE668 pKa = 3.88IQRR671 pKa = 11.84LGSKK675 pKa = 10.01KK676 pKa = 9.99IPYY679 pKa = 9.92SDD681 pKa = 4.79DD682 pKa = 4.37DD683 pKa = 4.53SFLHH687 pKa = 5.43AHH689 pKa = 6.21FMSFVDD695 pKa = 3.25RR696 pKa = 11.84LKK698 pKa = 10.69FLEE701 pKa = 4.45RR702 pKa = 11.84MSQFTIDD709 pKa = 3.66QLTLFYY715 pKa = 10.93EE716 pKa = 4.45MKK718 pKa = 9.82YY719 pKa = 10.41KK720 pKa = 10.73KK721 pKa = 10.34SGADD725 pKa = 3.08ASLVLGAFKK734 pKa = 10.79KK735 pKa = 9.65YY736 pKa = 5.61TTNFNAYY743 pKa = 9.19KK744 pKa = 10.07EE745 pKa = 5.0LYY747 pKa = 10.17FSDD750 pKa = 4.16RR751 pKa = 11.84HH752 pKa = 5.3QCDD755 pKa = 4.47LINSFSIGDD764 pKa = 3.84FVIKK768 pKa = 10.48LYY770 pKa = 10.14VEE772 pKa = 4.3KK773 pKa = 10.08PARR776 pKa = 11.84RR777 pKa = 3.59

Molecular weight: 87.55 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|C0MNB6|C0MNB6_9BROM Capsid protein OS=Gayfeather mild mottle virus OX=578305 GN=cp PE=3 SV=1
MM1 pKa = 7.18AQSGTGGSSRR11 pKa = 11.84RR12 pKa = 11.84PRR14 pKa = 11.84RR15 pKa = 11.84GRR17 pKa = 11.84RR18 pKa = 11.84NNNNRR23 pKa = 11.84NNDD26 pKa = 3.21RR27 pKa = 11.84DD28 pKa = 3.56KK29 pKa = 11.35ALRR32 pKa = 11.84DD33 pKa = 3.13LTVQVKK39 pKa = 9.96RR40 pKa = 11.84LAIIAASTAPSLQHH54 pKa = 5.5PTFIASQRR62 pKa = 11.84CRR64 pKa = 11.84PGYY67 pKa = 9.3TYY69 pKa = 10.71TSLDD73 pKa = 3.28VRR75 pKa = 11.84PTKK78 pKa = 9.23TEE80 pKa = 3.71KK81 pKa = 10.59GHH83 pKa = 6.88SFGQRR88 pKa = 11.84LTIPVPVSDD97 pKa = 4.09YY98 pKa = 10.35PKK100 pKa = 10.6KK101 pKa = 10.39KK102 pKa = 9.77VSCVQVRR109 pKa = 11.84LNPSPKK115 pKa = 9.1FDD117 pKa = 3.39STIWVSLRR125 pKa = 11.84RR126 pKa = 11.84LDD128 pKa = 3.37EE129 pKa = 4.21TTILTSEE136 pKa = 4.2NVFKK140 pKa = 11.3LFTDD144 pKa = 5.01GAASVLIYY152 pKa = 9.41QHH154 pKa = 5.69VTTGVQPNNKK164 pKa = 8.2ITFDD168 pKa = 3.6MSNVDD173 pKa = 4.76AEE175 pKa = 4.21IGDD178 pKa = 3.83MNRR181 pKa = 11.84YY182 pKa = 9.3ALIVYY187 pKa = 10.31SKK189 pKa = 11.29DD190 pKa = 3.38DD191 pKa = 3.49VLEE194 pKa = 4.46ADD196 pKa = 3.76EE197 pKa = 4.52MVIHH201 pKa = 6.73VDD203 pKa = 3.51VEE205 pKa = 4.22HH206 pKa = 6.35QRR208 pKa = 11.84IPSATSLPVV217 pKa = 2.91

Molecular weight: 24.28 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5

0

5

2382

120

988

476.4

53.52

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.549 ± 0.574

2.141 ± 0.153

7.095 ± 0.861

5.5 ± 0.326

5.122 ± 0.711

5.248 ± 0.444

3.107 ± 0.227

4.492 ± 0.396

6.003 ± 0.349

8.564 ± 0.402

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.519 ± 0.312

4.072 ± 0.219

4.492 ± 0.752

3.568 ± 0.191

5.206 ± 0.691

8.606 ± 0.925

6.549 ± 0.707

7.263 ± 0.281

0.924 ± 0.177

2.981 ± 0.257
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski