Thorsellia anophelis DSM 18579

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales; Thorselliaceae; Thorsellia; Thorsellia anophelis

Average proteome isoelectric point is 6.37

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2885 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1I0CBL5|A0A1I0CBL5_9GAMM ATP-dependent RNA helicase RhlB OS=Thorsellia anophelis DSM 18579 OX=1123402 GN=rhlB PE=3 SV=1
MM1 pKa = 7.64ASTYY5 pKa = 9.79PALTEE10 pKa = 3.99EE11 pKa = 4.09QIEE14 pKa = 4.53YY15 pKa = 10.57YY16 pKa = 10.01EE17 pKa = 4.05QIKK20 pKa = 10.5IEE22 pKa = 3.97TRR24 pKa = 11.84DD25 pKa = 3.41IIKK28 pKa = 10.63NLLDD32 pKa = 5.06DD33 pKa = 4.98GSLPDD38 pKa = 3.79ALYY41 pKa = 10.76LIEE44 pKa = 4.81HH45 pKa = 6.97HH46 pKa = 6.46FASDD50 pKa = 4.38DD51 pKa = 3.89FEE53 pKa = 4.41QLEE56 pKa = 4.23NLAVEE61 pKa = 4.32AFKK64 pKa = 11.22LDD66 pKa = 3.85FEE68 pKa = 4.61VTDD71 pKa = 3.86AEE73 pKa = 4.23EE74 pKa = 5.52LEE76 pKa = 4.59DD77 pKa = 3.78EE78 pKa = 4.3NGEE81 pKa = 4.32VFFACDD87 pKa = 3.58VVCEE91 pKa = 4.14MNLVAEE97 pKa = 5.11KK98 pKa = 9.94IDD100 pKa = 3.74EE101 pKa = 4.21QVEE104 pKa = 4.12LMIKK108 pKa = 10.43LAGKK112 pKa = 8.15FNCEE116 pKa = 3.21YY117 pKa = 10.55DD118 pKa = 2.91GWGTYY123 pKa = 10.14FEE125 pKa = 5.73DD126 pKa = 6.26GSDD129 pKa = 3.66EE130 pKa = 4.53DD131 pKa = 5.02DD132 pKa = 4.31GVIFDD137 pKa = 5.6DD138 pKa = 4.45EE139 pKa = 4.27

Molecular weight: 15.98 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1I0C427|A0A1I0C427_9GAMM CRISPR-associated protein Cas2 OS=Thorsellia anophelis DSM 18579 OX=1123402 GN=SAMN02583745_01476 PE=4 SV=1
MM1 pKa = 7.45KK2 pKa = 9.61RR3 pKa = 11.84TFQPSNTKK11 pKa = 10.11RR12 pKa = 11.84LRR14 pKa = 11.84THH16 pKa = 6.38GFRR19 pKa = 11.84ARR21 pKa = 11.84MATKK25 pKa = 10.37NGRR28 pKa = 11.84KK29 pKa = 9.2VLANRR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 10.28GRR39 pKa = 11.84ARR41 pKa = 11.84LTVSSKK47 pKa = 11.16

Molecular weight: 5.44 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2885

0

2885

965995

39

4569

334.8

37.44

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.573 ± 0.053

0.89 ± 0.016

5.334 ± 0.04

6.147 ± 0.04

4.427 ± 0.034

6.189 ± 0.051

2.102 ± 0.027

8.059 ± 0.045

6.043 ± 0.035

10.511 ± 0.068

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.33 ± 0.023

5.338 ± 0.052

3.906 ± 0.033

4.193 ± 0.034

4.009 ± 0.037

6.792 ± 0.041

5.738 ± 0.04

6.022 ± 0.036

1.145 ± 0.017

3.252 ± 0.03
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski