candidate division SR1 bacterium
Average proteome isoelectric point is 6.86
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 1229 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A2W4KAX4|A0A2W4KAX4_9BACT Uncharacterized protein OS=candidate division SR1 bacterium OX=2044938 GN=DLD55_06150 PE=3 SV=1
MM1 pKa = 7.74 CGLFLSIFCWFQIKK15 pKa = 9.57 EE16 pKa = 4.06 VLVPYY21 pKa = 10.24 FPEE24 pKa = 4.12 FTFFQYY30 pKa = 10.63 EE31 pKa = 4.16 SSSFSLKK38 pKa = 10.11 NQPILVKK45 pKa = 10.4 RR46 pKa = 11.84 EE47 pKa = 3.83 EE48 pKa = 3.98 IEE50 pKa = 3.98 YY51 pKa = 10.78 EE52 pKa = 3.93 EE53 pKa = 5.53 VIFTDD58 pKa = 3.46 EE59 pKa = 4.32 FDD61 pKa = 3.77 WNSSFF66 pKa = 4.94
Molecular weight: 8.07 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.955
IPC2_protein 4.279
IPC_protein 4.075
Toseland 3.935
ProMoST 4.177
Dawson 3.999
Bjellqvist 4.164
Wikipedia 3.859
Rodwell 3.923
Grimsley 3.859
Solomon 3.986
Lehninger 3.935
Nozaki 4.139
DTASelect 4.164
Thurlkill 3.961
EMBOSS 3.872
Sillero 4.177
Patrickios 1.914
IPC_peptide 3.999
IPC2_peptide 4.164
IPC2.peptide.svr19 4.091
Protein with the highest isoelectric point:
>tr|A0A2W4III7|A0A2W4III7_9BACT Uncharacterized protein OS=candidate division SR1 bacterium OX=2044938 GN=DLD55_04775 PE=4 SV=1
MM1 pKa = 7.27 LRR3 pKa = 11.84 KK4 pKa = 9.93 RR5 pKa = 11.84 MPGALLRR12 pKa = 11.84 AIWLVRR18 pKa = 11.84 LATLILRR25 pKa = 11.84 QEE27 pKa = 4.18 EE28 pKa = 4.1 LSRR31 pKa = 11.84 AKK33 pKa = 10.65 LEE35 pKa = 4.11 PFLWLLFTILLFIGFLEE52 pKa = 4.53 SKK54 pKa = 10.56 LPSKK58 pKa = 10.49 KK59 pKa = 9.87 LRR61 pKa = 11.84 RR62 pKa = 11.84 FGYY65 pKa = 9.65 FLIAIYY71 pKa = 10.75 YY72 pKa = 8.71 LFLMLFFGFVFLMVPWDD89 pKa = 3.93 YY90 pKa = 11.65 FEE92 pKa = 5.1 LWSLGLMIIFLIVYY106 pKa = 9.48 LGDD109 pKa = 3.24 GMVRR113 pKa = 11.84 RR114 pKa = 11.84 NIIRR118 pKa = 11.84 FCSQRR123 pKa = 11.84 KK124 pKa = 8.2 KK125 pKa = 10.98
Molecular weight: 15.12 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.328
IPC2_protein 9.94
IPC_protein 10.76
Toseland 10.76
ProMoST 10.54
Dawson 10.862
Bjellqvist 10.599
Wikipedia 11.096
Rodwell 11.096
Grimsley 10.921
Solomon 10.979
Lehninger 10.95
Nozaki 10.73
DTASelect 10.599
Thurlkill 10.76
EMBOSS 11.155
Sillero 10.804
Patrickios 10.833
IPC_peptide 10.979
IPC2_peptide 9.56
IPC2.peptide.svr19 8.377
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
1229
0
1229
360686
29
4309
293.5
33.32
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.118 ± 0.081
0.993 ± 0.034
4.757 ± 0.052
7.61 ± 0.076
5.321 ± 0.062
6.537 ± 0.07
1.787 ± 0.031
7.305 ± 0.063
8.606 ± 0.074
10.865 ± 0.094
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.138 ± 0.04
4.767 ± 0.076
3.493 ± 0.049
4.758 ± 0.06
3.728 ± 0.046
6.264 ± 0.061
5.112 ± 0.066
5.055 ± 0.058
0.901 ± 0.025
3.883 ± 0.051
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here