Odonata-associated circular virus-10
Average proteome isoelectric point is 8.49
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A0B4UI33|A0A0B4UI33_9VIRU Putative capsid protein OS=Odonata-associated circular virus-10 OX=1592110 PE=4 SV=1
MM1 pKa = 7.55 LFGVCFTFNNWSAEE15 pKa = 4.21 TKK17 pKa = 10.06 KK18 pKa = 10.7 ACQKK22 pKa = 10.97 AIGQAGILYY31 pKa = 8.95 IIWGEE36 pKa = 3.99 EE37 pKa = 3.74 VGEE40 pKa = 4.31 EE41 pKa = 4.39 GTPHH45 pKa = 5.67 LQGYY49 pKa = 6.92 IQCNQDD55 pKa = 2.4 KK56 pKa = 9.86 YY57 pKa = 10.26 KK58 pKa = 11.04 RR59 pKa = 11.84 LMRR62 pKa = 11.84 VIGTCHH68 pKa = 5.78 MEE70 pKa = 4.12 KK71 pKa = 10.43 QKK73 pKa = 9.85 GTSDD77 pKa = 3.5 EE78 pKa = 3.99 ARR80 pKa = 11.84 DD81 pKa = 3.69 YY82 pKa = 11.17 CKK84 pKa = 10.44 KK85 pKa = 10.67 DD86 pKa = 3.98 GIWQEE91 pKa = 3.47 HH92 pKa = 6.76 GIYY95 pKa = 10.46 KK96 pKa = 10.62 FIDD99 pKa = 3.71 APKK102 pKa = 9.26 KK103 pKa = 9.46 RR104 pKa = 11.84 QGEE107 pKa = 4.1 RR108 pKa = 11.84 SDD110 pKa = 4.61 LILVKK115 pKa = 10.67 KK116 pKa = 10.76 SIDD119 pKa = 3.23 EE120 pKa = 4.26 GMSYY124 pKa = 11.55 DD125 pKa = 5.28 DD126 pKa = 3.28 ICEE129 pKa = 3.8 KK130 pKa = 10.8 HH131 pKa = 5.48 FAIVGKK137 pKa = 8.17 YY138 pKa = 9.17 SRR140 pKa = 11.84 FIKK143 pKa = 10.37 EE144 pKa = 3.53 RR145 pKa = 11.84 VQARR149 pKa = 11.84 DD150 pKa = 3.02 SKK152 pKa = 8.92 TQEE155 pKa = 3.98 TEE157 pKa = 3.81 LQEE160 pKa = 4.12 QFVNSSLRR168 pKa = 11.84 PWQQKK173 pKa = 10.44 LLDD176 pKa = 3.59 ITATEE181 pKa = 3.98 ACPRR185 pKa = 11.84 KK186 pKa = 9.62 IHH188 pKa = 6.66 WIWEE192 pKa = 4.09 DD193 pKa = 2.96 KK194 pKa = 11.42 GNVGKK199 pKa = 10.87 SFMTTYY205 pKa = 10.54 LGVMKK210 pKa = 10.61 GSALLTAGKK219 pKa = 9.31 KK220 pKa = 10.41 ADD222 pKa = 3.38 MAYY225 pKa = 9.56 IYY227 pKa = 10.08 AQKK230 pKa = 9.89 PSKK233 pKa = 10.19 IVCFDD238 pKa = 4.19 LARR241 pKa = 11.84 TNEE244 pKa = 3.94 EE245 pKa = 4.17 YY246 pKa = 11.19 LDD248 pKa = 3.96 GVYY251 pKa = 10.81 SLAEE255 pKa = 3.9 DD256 pKa = 4.11 LKK258 pKa = 10.22 NGRR261 pKa = 11.84 VVSTKK266 pKa = 10.49 YY267 pKa = 9.8 EE268 pKa = 4.14 SKK270 pKa = 9.47 TAFFKK275 pKa = 10.58 PPHH278 pKa = 6.01 VFVFANFLPNMKK290 pKa = 9.49 KK291 pKa = 9.37 WSAYY295 pKa = 9.24 RR296 pKa = 11.84 YY297 pKa = 10.36 NIIDD301 pKa = 4.25 LNRR304 pKa = 11.84 PLIKK308 pKa = 10.59 LPILPIVLCIDD319 pKa = 4.16 CARR322 pKa = 11.84 CDD324 pKa = 3.78 VVCIQSTIQVRR335 pKa = 11.84 PDD337 pKa = 3.15 YY338 pKa = 9.73 PHH340 pKa = 6.2 NHH342 pKa = 6.79 RR343 pKa = 11.84 IVYY346 pKa = 9.59
Molecular weight: 39.92 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 7.832
IPC2_protein 8.039
IPC_protein 7.849
Toseland 7.6
ProMoST 8.507
Dawson 8.639
Bjellqvist 8.902
Wikipedia 8.595
Rodwell 8.697
Grimsley 7.571
Solomon 8.712
Lehninger 8.712
Nozaki 9.092
DTASelect 8.653
Thurlkill 8.726
EMBOSS 8.829
Sillero 9.019
Patrickios 4.279
IPC_peptide 8.712
IPC2_peptide 7.732
IPC2.peptide.svr19 7.962
Protein with the highest isoelectric point:
>tr|A0A0B4UI33|A0A0B4UI33_9VIRU Putative capsid protein OS=Odonata-associated circular virus-10 OX=1592110 PE=4 SV=1
MM1 pKa = 7.66 INRR4 pKa = 11.84 AVMKK8 pKa = 10.55 KK9 pKa = 10.16 SEE11 pKa = 4.34 TKK13 pKa = 10.54 NLVASSEE20 pKa = 4.17 NIQLYY25 pKa = 9.54 HH26 pKa = 6.67 NNGAVSGAGGSTAAVIFNPWDD47 pKa = 4.35 AIGQGLTVRR56 pKa = 11.84 NRR58 pKa = 11.84 IGNEE62 pKa = 3.21 IYY64 pKa = 10.0 PRR66 pKa = 11.84 GMAVRR71 pKa = 11.84 MEE73 pKa = 4.74 LSNKK77 pKa = 9.56 LDD79 pKa = 3.81 RR80 pKa = 11.84 PNLTYY85 pKa = 10.54 RR86 pKa = 11.84 IIVAVIPKK94 pKa = 9.5 YY95 pKa = 10.8 YY96 pKa = 10.76 NNTVSSAAFDD106 pKa = 3.9 PRR108 pKa = 11.84 DD109 pKa = 3.77 NNASMNWMISWWKK122 pKa = 8.79 QDD124 pKa = 3.11 SGIKK128 pKa = 9.73 ILYY131 pKa = 8.85 DD132 pKa = 3.37 KK133 pKa = 10.66 SARR136 pKa = 11.84 AQLGFYY142 pKa = 9.99 AVPSADD148 pKa = 3.27 GDD150 pKa = 3.81 QDD152 pKa = 3.4 GKK154 pKa = 10.76 EE155 pKa = 3.73 ASKK158 pKa = 10.91 VVKK161 pKa = 10.33 LYY163 pKa = 10.11 IKK165 pKa = 10.12 SRR167 pKa = 11.84 RR168 pKa = 11.84 GQKK171 pKa = 10.51 LMWGQDD177 pKa = 3.12 QRR179 pKa = 11.84 LVNNPMIMWVIRR191 pKa = 11.84 SDD193 pKa = 4.43 LYY195 pKa = 10.95 GTLNTDD201 pKa = 3.08 NVASCAINTKK211 pKa = 10.25 HH212 pKa = 5.3 YY213 pKa = 9.97 WKK215 pKa = 10.64 DD216 pKa = 3.06 RR217 pKa = 3.72
Molecular weight: 24.45 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.149
IPC2_protein 9.253
IPC_protein 9.253
Toseland 9.809
ProMoST 9.633
Dawson 10.072
Bjellqvist 9.75
Wikipedia 10.248
Rodwell 10.409
Grimsley 10.16
Solomon 10.101
Lehninger 10.058
Nozaki 9.809
DTASelect 9.75
Thurlkill 9.897
EMBOSS 10.233
Sillero 9.984
Patrickios 7.6
IPC_peptide 10.087
IPC2_peptide 8.331
IPC2.peptide.svr19 8.302
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2
0
2
563
217
346
281.5
32.19
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
7.105 ± 1.006
2.309 ± 1.126
5.861 ± 0.079
4.618 ± 1.41
3.02 ± 0.997
6.394 ± 0.316
1.776 ± 0.521
7.815 ± 0.269
8.703 ± 1.091
6.394 ± 0.246
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
3.02 ± 0.687
5.684 ± 2.152
3.552 ± 0.199
4.44 ± 0.459
5.506 ± 0.576
6.039 ± 1.094
4.263 ± 0.351
6.217 ± 0.424
2.487 ± 0.45
4.796 ± 0.114
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here