Amino acid dipepetide frequency for Streptomyces sp. SID10853

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
21.49AlaAla: 21.49 ± 0.153
1.026AlaCys: 1.026 ± 0.024
8.588AlaAsp: 8.588 ± 0.071
8.058AlaGlu: 8.058 ± 0.078
3.484AlaPhe: 3.484 ± 0.043
13.994AlaGly: 13.994 ± 0.101
2.939AlaHis: 2.939 ± 0.035
3.55AlaIle: 3.55 ± 0.042
2.916AlaLys: 2.916 ± 0.055
14.182AlaLeu: 14.182 ± 0.118
2.689AlaMet: 2.689 ± 0.042
1.885AlaAsn: 1.885 ± 0.034
7.09AlaPro: 7.09 ± 0.067
3.825AlaGln: 3.825 ± 0.048
9.771AlaArg: 9.771 ± 0.074
5.941AlaSer: 5.941 ± 0.062
6.882AlaThr: 6.882 ± 0.056
12.431AlaVal: 12.431 ± 0.087
1.692AlaTrp: 1.692 ± 0.027
2.724AlaTyr: 2.724 ± 0.036
0.0AlaXaa: 0.0 ± 0.0
Cys
1.08CysAla: 1.08 ± 0.025
0.098CysCys: 0.098 ± 0.008
0.475CysAsp: 0.475 ± 0.017
0.374CysGlu: 0.374 ± 0.013
0.235CysPhe: 0.235 ± 0.011
0.972CysGly: 0.972 ± 0.022
0.182CysHis: 0.182 ± 0.009
0.2CysIle: 0.2 ± 0.01
0.109CysLys: 0.109 ± 0.008
0.711CysLeu: 0.711 ± 0.018
0.136CysMet: 0.136 ± 0.007
0.148CysAsn: 0.148 ± 0.009
0.469CysPro: 0.469 ± 0.017
0.16CysGln: 0.16 ± 0.01
0.586CysArg: 0.586 ± 0.016
0.446CysSer: 0.446 ± 0.014
0.56CysThr: 0.56 ± 0.018
0.671CysVal: 0.671 ± 0.018
0.124CysTrp: 0.124 ± 0.008
0.162CysTyr: 0.162 ± 0.009
0.0CysXaa: 0.0 ± 0.0
Asp
7.507AspAla: 7.507 ± 0.068
0.397AspCys: 0.397 ± 0.016
3.417AspAsp: 3.417 ± 0.04
3.857AspGlu: 3.857 ± 0.047
1.666AspPhe: 1.666 ± 0.025
6.384AspGly: 6.384 ± 0.068
1.424AspHis: 1.424 ± 0.025
2.099AspIle: 2.099 ± 0.03
1.186AspLys: 1.186 ± 0.03
5.829AspLeu: 5.829 ± 0.056
0.85AspMet: 0.85 ± 0.019
0.951AspAsn: 0.951 ± 0.022
4.365AspPro: 4.365 ± 0.051
1.593AspGln: 1.593 ± 0.032
4.782AspArg: 4.782 ± 0.053
2.8AspSer: 2.8 ± 0.039
3.302AspThr: 3.302 ± 0.039
4.532AspVal: 4.532 ± 0.051
0.997AspTrp: 0.997 ± 0.024
1.058AspTyr: 1.058 ± 0.022
0.0AspXaa: 0.0 ± 0.0
Glu
6.736GluAla: 6.736 ± 0.071
0.37GluCys: 0.37 ± 0.012
2.369GluAsp: 2.369 ± 0.038
3.097GluGlu: 3.097 ± 0.047
1.448GluPhe: 1.448 ± 0.029
4.009GluGly: 4.009 ± 0.042
1.442GluHis: 1.442 ± 0.025
2.269GluIle: 2.269 ± 0.033
1.389GluLys: 1.389 ± 0.029
6.67GluLeu: 6.67 ± 0.064
0.908GluMet: 0.908 ± 0.022
1.036GluAsn: 1.036 ± 0.022
3.204GluPro: 3.204 ± 0.04
2.273GluGln: 2.273 ± 0.036
5.076GluArg: 5.076 ± 0.061
2.697GluSer: 2.697 ± 0.038
2.824GluThr: 2.824 ± 0.034
4.106GluVal: 4.106 ± 0.052
0.738GluTrp: 0.738 ± 0.019
1.101GluTyr: 1.101 ± 0.023
0.0GluXaa: 0.0 ± 0.0
Phe
3.815PheAla: 3.815 ± 0.038
0.284PheCys: 0.284 ± 0.011
1.988PheAsp: 1.988 ± 0.032
1.324PheGlu: 1.324 ± 0.026
0.925PhePhe: 0.925 ± 0.024
3.176PheGly: 3.176 ± 0.042
0.639PheHis: 0.639 ± 0.017
0.866PheIle: 0.866 ± 0.022
0.515PheLys: 0.515 ± 0.017
2.596PheLeu: 2.596 ± 0.041
0.438PheMet: 0.438 ± 0.016
0.59PheAsn: 0.59 ± 0.017
1.439PhePro: 1.439 ± 0.026
0.699PheGln: 0.699 ± 0.019
1.811PheArg: 1.811 ± 0.03
1.623PheSer: 1.623 ± 0.026
2.132PheThr: 2.132 ± 0.033
2.205PheVal: 2.205 ± 0.034
0.423PheTrp: 0.423 ± 0.013
0.585PheTyr: 0.585 ± 0.019
0.0PheXaa: 0.0 ± 0.0
Gly
11.833GlyAla: 11.833 ± 0.087
0.876GlyCys: 0.876 ± 0.021
5.107GlyAsp: 5.107 ± 0.055
4.892GlyGlu: 4.892 ± 0.052
3.034GlyPhe: 3.034 ± 0.047
9.138GlyGly: 9.138 ± 0.1
2.415GlyHis: 2.415 ± 0.035
3.766GlyIle: 3.766 ± 0.041
2.543GlyLys: 2.543 ± 0.042
9.784GlyLeu: 9.784 ± 0.077
2.104GlyMet: 2.104 ± 0.031
1.777GlyAsn: 1.777 ± 0.035
5.449GlyPro: 5.449 ± 0.061
2.855GlyGln: 2.855 ± 0.038
7.816GlyArg: 7.816 ± 0.065
5.965GlySer: 5.965 ± 0.065
6.54GlyThr: 6.54 ± 0.065
7.595GlyVal: 7.595 ± 0.071
1.723GlyTrp: 1.723 ± 0.034
2.301GlyTyr: 2.301 ± 0.032
0.0GlyXaa: 0.0 ± 0.0
His
2.661HisAla: 2.661 ± 0.039
0.221HisCys: 0.221 ± 0.01
1.314HisAsp: 1.314 ± 0.024
1.165HisGlu: 1.165 ± 0.026
0.733HisPhe: 0.733 ± 0.018
2.533HisGly: 2.533 ± 0.036
0.733HisHis: 0.733 ± 0.021
0.811HisIle: 0.811 ± 0.019
0.37HisLys: 0.37 ± 0.013
2.337HisLeu: 2.337 ± 0.035
0.364HisMet: 0.364 ± 0.014
0.4HisAsn: 0.4 ± 0.015
1.836HisPro: 1.836 ± 0.027
0.698HisGln: 0.698 ± 0.019
2.139HisArg: 2.139 ± 0.034
1.177HisSer: 1.177 ± 0.021
1.487HisThr: 1.487 ± 0.029
1.595HisVal: 1.595 ± 0.025
0.388HisTrp: 0.388 ± 0.014
0.478HisTyr: 0.478 ± 0.015
0.0HisXaa: 0.0 ± 0.0
Ile
5.106IleAla: 5.106 ± 0.055
0.343IleCys: 0.343 ± 0.013
2.365IleAsp: 2.365 ± 0.03
2.048IleGlu: 2.048 ± 0.033
0.765IlePhe: 0.765 ± 0.019
4.065IleGly: 4.065 ± 0.05
0.653IleHis: 0.653 ± 0.019
0.972IleIle: 0.972 ± 0.023
0.755IleLys: 0.755 ± 0.021
2.435IleLeu: 2.435 ± 0.034
0.487IleMet: 0.487 ± 0.016
0.749IleAsn: 0.749 ± 0.018
1.853IlePro: 1.853 ± 0.034
0.786IleGln: 0.786 ± 0.017
2.309IleArg: 2.309 ± 0.031
1.89IleSer: 1.89 ± 0.028
2.332IleThr: 2.332 ± 0.031
2.85IleVal: 2.85 ± 0.042
0.397IleTrp: 0.397 ± 0.013
0.559IleTyr: 0.559 ± 0.017
0.0IleXaa: 0.0 ± 0.0
Lys
2.86LysAla: 2.86 ± 0.048
0.123LysCys: 0.123 ± 0.007
1.383LysAsp: 1.383 ± 0.03
1.132LysGlu: 1.132 ± 0.025
0.487LysPhe: 0.487 ± 0.017
1.887LysGly: 1.887 ± 0.036
0.45LysHis: 0.45 ± 0.013
0.879LysIle: 0.879 ± 0.019
0.94LysLys: 0.94 ± 0.036
2.068LysLeu: 2.068 ± 0.038
0.417LysMet: 0.417 ± 0.015
0.558LysAsn: 0.558 ± 0.019
1.388LysPro: 1.388 ± 0.031
0.762LysGln: 0.762 ± 0.02
1.396LysArg: 1.396 ± 0.029
1.286LysSer: 1.286 ± 0.027
1.286LysThr: 1.286 ± 0.028
1.915LysVal: 1.915 ± 0.036
0.284LysTrp: 0.284 ± 0.012
0.487LysTyr: 0.487 ± 0.017
0.0LysXaa: 0.0 ± 0.0
Leu
14.535LeuAla: 14.535 ± 0.099
0.834LeuCys: 0.834 ± 0.019
6.506LeuAsp: 6.506 ± 0.053
4.397LeuGlu: 4.397 ± 0.045
2.746LeuPhe: 2.746 ± 0.041
9.41LeuGly: 9.41 ± 0.08
2.437LeuHis: 2.437 ± 0.033
3.552LeuIle: 3.552 ± 0.046
2.016LeuLys: 2.016 ± 0.035
11.161LeuLeu: 11.161 ± 0.119
1.713LeuMet: 1.713 ± 0.029
1.777LeuAsn: 1.777 ± 0.028
6.419LeuPro: 6.419 ± 0.056
2.249LeuGln: 2.249 ± 0.034
8.335LeuArg: 8.335 ± 0.082
5.632LeuSer: 5.632 ± 0.05
6.844LeuThr: 6.844 ± 0.062
8.725LeuVal: 8.725 ± 0.073
1.208LeuTrp: 1.208 ± 0.027
1.836LeuTyr: 1.836 ± 0.033
0.0LeuXaa: 0.0 ± 0.0
Met
2.349MetAla: 2.349 ± 0.037
0.155MetCys: 0.155 ± 0.008
0.923MetAsp: 0.923 ± 0.024
0.772MetGlu: 0.772 ± 0.017
0.466MetPhe: 0.466 ± 0.016
1.37MetGly: 1.37 ± 0.023
0.393MetHis: 0.393 ± 0.012
0.706MetIle: 0.706 ± 0.021
0.46MetLys: 0.46 ± 0.014
1.726MetLeu: 1.726 ± 0.027
0.342MetMet: 0.342 ± 0.011
0.481MetAsn: 0.481 ± 0.017
1.158MetPro: 1.158 ± 0.024
0.47MetGln: 0.47 ± 0.015
1.429MetArg: 1.429 ± 0.026
1.403MetSer: 1.403 ± 0.026
1.59MetThr: 1.59 ± 0.03
1.383MetVal: 1.383 ± 0.023
0.226MetTrp: 0.226 ± 0.01
0.362MetTyr: 0.362 ± 0.012
0.0MetXaa: 0.0 ± 0.0
Asn
2.194AsnAla: 2.194 ± 0.039
0.155AsnCys: 0.155 ± 0.009
0.961AsnAsp: 0.961 ± 0.021
0.814AsnGlu: 0.814 ± 0.022
0.491AsnPhe: 0.491 ± 0.014
1.942AsnGly: 1.942 ± 0.035
0.406AsnHis: 0.406 ± 0.015
0.706AsnIle: 0.706 ± 0.019
0.459AsnLys: 0.459 ± 0.016
1.645AsnLeu: 1.645 ± 0.027
0.31AsnMet: 0.31 ± 0.012
0.429AsnAsn: 0.429 ± 0.014
1.329AsnPro: 1.329 ± 0.024
0.523AsnGln: 0.523 ± 0.014
1.28AsnArg: 1.28 ± 0.028
1.003AsnSer: 1.003 ± 0.024
1.186AsnThr: 1.186 ± 0.027
1.347AsnVal: 1.347 ± 0.027
0.304AsnTrp: 0.304 ± 0.012
0.423AsnTyr: 0.423 ± 0.015
0.0AsnXaa: 0.0 ± 0.0
Pro
8.744ProAla: 8.744 ± 0.076
0.321ProCys: 0.321 ± 0.011
4.386ProAsp: 4.386 ± 0.049
3.956ProGlu: 3.956 ± 0.045
1.545ProPhe: 1.545 ± 0.025
6.885ProGly: 6.885 ± 0.066
1.426ProHis: 1.426 ± 0.027
1.181ProIle: 1.181 ± 0.025
1.184ProLys: 1.184 ± 0.029
5.394ProLeu: 5.394 ± 0.057
1.012ProMet: 1.012 ± 0.022
0.864ProAsn: 0.864 ± 0.02
3.299ProPro: 3.299 ± 0.051
1.97ProGln: 1.97 ± 0.046
3.699ProArg: 3.699 ± 0.041
3.191ProSer: 3.191 ± 0.044
2.935ProThr: 2.935 ± 0.038
5.683ProVal: 5.683 ± 0.065
0.821ProTrp: 0.821 ± 0.021
1.507ProTyr: 1.507 ± 0.029
0.0ProXaa: 0.0 ± 0.0
Gln
3.588GlnAla: 3.588 ± 0.046
0.188GlnCys: 0.188 ± 0.009
1.515GlnAsp: 1.515 ± 0.027
1.53GlnGlu: 1.53 ± 0.029
0.707GlnPhe: 0.707 ± 0.019
2.427GlnGly: 2.427 ± 0.035
0.758GlnHis: 0.758 ± 0.02
1.115GlnIle: 1.115 ± 0.022
0.678GlnLys: 0.678 ± 0.022
3.28GlnLeu: 3.28 ± 0.042
0.518GlnMet: 0.518 ± 0.018
0.521GlnAsn: 0.521 ± 0.016
1.728GlnPro: 1.728 ± 0.039
1.455GlnGln: 1.455 ± 0.041
2.292GlnArg: 2.292 ± 0.038
1.404GlnSer: 1.404 ± 0.024
1.371GlnThr: 1.371 ± 0.028
2.397GlnVal: 2.397 ± 0.038
0.461GlnTrp: 0.461 ± 0.016
0.683GlnTyr: 0.683 ± 0.021
0.0GlnXaa: 0.0 ± 0.0
Arg
9.418ArgAla: 9.418 ± 0.079
0.556ArgCys: 0.556 ± 0.017
4.095ArgAsp: 4.095 ± 0.043
4.478ArgGlu: 4.478 ± 0.051
2.321ArgPhe: 2.321 ± 0.035
5.692ArgGly: 5.692 ± 0.053
1.968ArgHis: 1.968 ± 0.033
3.476ArgIle: 3.476 ± 0.04
1.66ArgLys: 1.66 ± 0.029
8.38ArgLeu: 8.38 ± 0.072
1.724ArgMet: 1.724 ± 0.027
1.379ArgAsn: 1.379 ± 0.026
4.67ArgPro: 4.67 ± 0.053
2.196ArgGln: 2.196 ± 0.031
7.08ArgArg: 7.08 ± 0.068
4.168ArgSer: 4.168 ± 0.048
5.708ArgThr: 5.708 ± 0.054
5.432ArgVal: 5.432 ± 0.05
1.286ArgTrp: 1.286 ± 0.025
1.753ArgTyr: 1.753 ± 0.027
0.0ArgXaa: 0.0 ± 0.0
Ser
7.155SerAla: 7.155 ± 0.069
0.424SerCys: 0.424 ± 0.015
2.905SerAsp: 2.905 ± 0.041
2.381SerGlu: 2.381 ± 0.036
1.64SerPhe: 1.64 ± 0.027
6.518SerGly: 6.518 ± 0.066
1.151SerHis: 1.151 ± 0.022
1.545SerIle: 1.545 ± 0.024
1.155SerLys: 1.155 ± 0.028
5.031SerLeu: 5.031 ± 0.041
1.133SerMet: 1.133 ± 0.022
0.91SerAsn: 0.91 ± 0.023
3.215SerPro: 3.215 ± 0.041
1.336SerGln: 1.336 ± 0.027
3.78SerArg: 3.78 ± 0.041
3.196SerSer: 3.196 ± 0.055
3.324SerThr: 3.324 ± 0.047
4.511SerVal: 4.511 ± 0.045
0.923SerTrp: 0.923 ± 0.023
1.308SerTyr: 1.308 ± 0.025
0.0SerXaa: 0.0 ± 0.0
Thr
9.252ThrAla: 9.252 ± 0.081
0.41ThrCys: 0.41 ± 0.014
3.749ThrAsp: 3.749 ± 0.047
3.205ThrGlu: 3.205 ± 0.043
1.706ThrPhe: 1.706 ± 0.028
7.255ThrGly: 7.255 ± 0.063
1.233ThrHis: 1.233 ± 0.023
1.708ThrIle: 1.708 ± 0.029
1.161ThrLys: 1.161 ± 0.026
5.756ThrLeu: 5.756 ± 0.054
0.96ThrMet: 0.96 ± 0.021
0.943ThrAsn: 0.943 ± 0.025
4.016ThrPro: 4.016 ± 0.048
1.327ThrGln: 1.327 ± 0.024
3.755ThrArg: 3.755 ± 0.044
3.272ThrSer: 3.272 ± 0.038
3.757ThrThr: 3.757 ± 0.05
6.262ThrVal: 6.262 ± 0.058
0.827ThrTrp: 0.827 ± 0.019
1.305ThrTyr: 1.305 ± 0.025
0.0ThrXaa: 0.0 ± 0.0
Val
10.468ValAla: 10.468 ± 0.086
0.785ValCys: 0.785 ± 0.018
4.701ValAsp: 4.701 ± 0.046
4.41ValGlu: 4.41 ± 0.044
2.459ValPhe: 2.459 ± 0.038
6.502ValGly: 6.502 ± 0.056
2.016ValHis: 2.016 ± 0.029
3.2ValIle: 3.2 ± 0.046
1.715ValLys: 1.715 ± 0.033
9.488ValLeu: 9.488 ± 0.079
1.499ValMet: 1.499 ± 0.03
1.706ValAsn: 1.706 ± 0.034
5.264ValPro: 5.264 ± 0.05
2.217ValGln: 2.217 ± 0.033
7.082ValArg: 7.082 ± 0.06
4.505ValSer: 4.505 ± 0.049
5.546ValThr: 5.546 ± 0.059
7.787ValVal: 7.787 ± 0.067
1.099ValTrp: 1.099 ± 0.023
1.556ValTyr: 1.556 ± 0.025
0.0ValXaa: 0.0 ± 0.0
Trp
1.555TrpAla: 1.555 ± 0.027
0.148TrpCys: 0.148 ± 0.009
0.806TrpAsp: 0.806 ± 0.021
0.675TrpGlu: 0.675 ± 0.02
0.515TrpPhe: 0.515 ± 0.016
1.048TrpGly: 1.048 ± 0.025
0.369TrpHis: 0.369 ± 0.014
0.552TrpIle: 0.552 ± 0.018
0.387TrpLys: 0.387 ± 0.013
1.73TrpLeu: 1.73 ± 0.03
0.303TrpMet: 0.303 ± 0.013
0.401TrpAsn: 0.401 ± 0.015
0.746TrpPro: 0.746 ± 0.02
0.631TrpGln: 0.631 ± 0.02
1.197TrpArg: 1.197 ± 0.026
0.929TrpSer: 0.929 ± 0.019
1.013TrpThr: 1.013 ± 0.022
0.933TrpVal: 0.933 ± 0.02
0.306TrpTrp: 0.306 ± 0.011
0.339TrpTyr: 0.339 ± 0.013
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.811TyrAla: 2.811 ± 0.035
0.184TyrCys: 0.184 ± 0.009
1.444TyrAsp: 1.444 ± 0.03
1.197TyrGlu: 1.197 ± 0.024
0.697TyrPhe: 0.697 ± 0.019
2.307TyrGly: 2.307 ± 0.034
0.407TyrHis: 0.407 ± 0.015
0.563TyrIle: 0.563 ± 0.017
0.389TyrLys: 0.389 ± 0.013
2.13TyrLeu: 2.13 ± 0.033
0.257TyrMet: 0.257 ± 0.011
0.404TyrAsn: 0.404 ± 0.014
1.066TyrPro: 1.066 ± 0.022
0.591TyrGln: 0.591 ± 0.019
1.826TyrArg: 1.826 ± 0.027
1.012TyrSer: 1.012 ± 0.023
1.24TyrThr: 1.24 ± 0.026
1.648TyrVal: 1.648 ± 0.026
0.355TyrTrp: 0.355 ± 0.013
0.472TyrTyr: 0.472 ± 0.016
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 7031 proteins (2203673 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski