Weissella oryzae (strain DSM 25784 / JCM 18191 / SG25)
Average proteome isoelectric point is 6.45
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2217 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A069CXA8|A0A069CXA8_WEIOS Phage head-to-tail connecting protein OS=Weissella oryzae (strain DSM 25784 / JCM 18191 / SG25) OX=1329250 GN=WOSG25_230150 PE=4 SV=1
MM1 pKa = 7.33 LVYY4 pKa = 10.09 IEE6 pKa = 5.57 KK7 pKa = 9.2 YY8 pKa = 8.75 TIANILQTLNGLKK21 pKa = 10.17 YY22 pKa = 10.46 SHH24 pKa = 6.5 SQGDD28 pKa = 3.68 FTGTKK33 pKa = 9.63 SGAVTLIYY41 pKa = 10.38 EE42 pKa = 4.29 VDD44 pKa = 3.43 QASVSDD50 pKa = 4.18 SDD52 pKa = 4.22 SMSDD56 pKa = 3.34 SEE58 pKa = 5.08 SISEE62 pKa = 4.36 SVSEE66 pKa = 4.74 SISSSGLTLEE76 pKa = 4.9 SMSDD80 pKa = 3.4 LTNGSDD86 pKa = 4.38 SLPHH90 pKa = 6.48 NNSLPNTGMVDD101 pKa = 3.61 EE102 pKa = 5.19 VSLQVFSS109 pKa = 4.21
Molecular weight: 11.59 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.759
IPC2_protein 3.884
IPC_protein 3.808
Toseland 3.617
ProMoST 3.973
Dawson 3.795
Bjellqvist 3.961
Wikipedia 3.732
Rodwell 3.643
Grimsley 3.528
Solomon 3.77
Lehninger 3.732
Nozaki 3.923
DTASelect 4.113
Thurlkill 3.681
EMBOSS 3.745
Sillero 3.923
Patrickios 1.888
IPC_peptide 3.77
IPC2_peptide 3.897
IPC2.peptide.svr19 3.823
Protein with the highest isoelectric point:
>tr|A0A069CUA8|A0A069CUA8_WEIOS Uncharacterized protein OS=Weissella oryzae (strain DSM 25784 / JCM 18191 / SG25) OX=1329250 GN=WOSG25_050450 PE=4 SV=1
MM1 pKa = 7.62 LMGIRR6 pKa = 11.84 KK7 pKa = 9.86 SIRR10 pKa = 11.84 LSMLMGIRR18 pKa = 11.84 KK19 pKa = 9.9 SIRR22 pKa = 11.84 LSMLMGIRR30 pKa = 11.84 KK31 pKa = 9.9 SIRR34 pKa = 11.84 LSMLMGIRR42 pKa = 11.84 KK43 pKa = 9.9 SIRR46 pKa = 11.84 LSMLMGIRR54 pKa = 11.84 KK55 pKa = 9.9 SIRR58 pKa = 11.84 LSMLLGICMSIRR70 pKa = 11.84 LSMFKK75 pKa = 10.5 RR76 pKa = 11.84 ITQVILKK83 pKa = 10.05 SITRR87 pKa = 11.84 RR88 pKa = 11.84 II89 pKa = 3.62
Molecular weight: 10.36 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.472
IPC2_protein 11.038
IPC_protein 12.618
Toseland 12.793
ProMoST 13.276
Dawson 12.793
Bjellqvist 12.778
Wikipedia 13.261
Rodwell 12.471
Grimsley 12.822
Solomon 13.276
Lehninger 13.188
Nozaki 12.778
DTASelect 12.778
Thurlkill 12.778
EMBOSS 13.29
Sillero 12.778
Patrickios 12.193
IPC_peptide 13.29
IPC2_peptide 12.266
IPC2.peptide.svr19 9.097
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2217
0
2217
613410
20
3736
276.7
30.71
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
9.168 ± 0.138
0.175 ± 0.009
5.785 ± 0.054
5.78 ± 0.07
4.276 ± 0.056
6.593 ± 0.06
1.691 ± 0.022
7.02 ± 0.056
6.063 ± 0.067
9.926 ± 0.082
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.668 ± 0.031
5.167 ± 0.052
3.281 ± 0.034
4.487 ± 0.047
3.983 ± 0.06
6.14 ± 0.105
6.215 ± 0.07
7.061 ± 0.038
1.092 ± 0.018
3.416 ± 0.035
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here