Pelagerythrobacter marensis
Average proteome isoelectric point is 6.02
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2659 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A0G3X824|A0A0G3X824_9SPHN Molybdopterin molybdenumtransferase OS=Pelagerythrobacter marensis OX=543877 GN=AM2010_1279 PE=3 SV=1
MM1 pKa = 7.18 KK2 pKa = 9.69 TIQRR6 pKa = 11.84 SIVLGCGLAILAGCGADD23 pKa = 4.05 EE24 pKa = 4.55 ISSPGTGGNVIINPPATPPPPPPPPPPPPATVSPAAGCPTIADD67 pKa = 3.88 PQGLLDD73 pKa = 4.58 GGTISGPTGEE83 pKa = 4.24 YY84 pKa = 9.94 RR85 pKa = 11.84 ICEE88 pKa = 3.94 LPEE91 pKa = 3.95 RR92 pKa = 11.84 FNASSTLPYY101 pKa = 10.56 VEE103 pKa = 4.27 GLLYY107 pKa = 10.96 FMDD110 pKa = 3.61 GRR112 pKa = 11.84 VDD114 pKa = 3.25 VGTDD118 pKa = 3.65 GGPTASNADD127 pKa = 3.33 TDD129 pKa = 3.89 VTLTIEE135 pKa = 4.39 PGAMFYY141 pKa = 10.25 ATGSSFLMVNRR152 pKa = 11.84 GNQINAVGTEE162 pKa = 3.94 DD163 pKa = 3.44 MPIIFTSRR171 pKa = 11.84 DD172 pKa = 3.19 NVLGINGEE180 pKa = 4.1 NSSGQWGGVVLAGRR194 pKa = 11.84 APITDD199 pKa = 3.3 CSGPNTTPGTVNCEE213 pKa = 4.04 RR214 pKa = 11.84 QVEE217 pKa = 4.57 GATDD221 pKa = 3.29 TALYY225 pKa = 10.61 GGATDD230 pKa = 4.21 NDD232 pKa = 3.98 SSGTMSYY239 pKa = 10.08 VQIRR243 pKa = 11.84 YY244 pKa = 9.98 SGFVLSGNNEE254 pKa = 4.1 LQSLTTGGTGTGTEE268 pKa = 4.36 LSHH271 pKa = 7.33 IMSYY275 pKa = 10.97 NSSDD279 pKa = 3.59 DD280 pKa = 3.45 GVEE283 pKa = 4.05 FFGGHH288 pKa = 4.73 VNLKK292 pKa = 9.78 NLIVVGAEE300 pKa = 4.01 DD301 pKa = 5.04 DD302 pKa = 5.12 ALDD305 pKa = 3.68 TDD307 pKa = 4.24 TGVKK311 pKa = 10.84 ANMQYY316 pKa = 10.99 VIAISRR322 pKa = 11.84 ANAGDD327 pKa = 3.88 TIIEE331 pKa = 4.19 ADD333 pKa = 3.64 STNTNIEE340 pKa = 3.75 NTPRR344 pKa = 11.84 QNTQISNATFIYY356 pKa = 10.16 NGAVRR361 pKa = 11.84 DD362 pKa = 3.45 QAIRR366 pKa = 11.84 IRR368 pKa = 11.84 GGADD372 pKa = 3.07 YY373 pKa = 11.01 AIVNSVLVDD382 pKa = 3.48 ATGSTPCIRR391 pKa = 11.84 IDD393 pKa = 3.51 DD394 pKa = 4.33 TEE396 pKa = 4.24 TTRR399 pKa = 11.84 AANGGLDD406 pKa = 3.89 DD407 pKa = 4.39 VGPPVFNSFVMDD419 pKa = 4.4 CATGFRR425 pKa = 11.84 NGSNGVSAADD435 pKa = 3.33 AQAIYY440 pKa = 10.68 DD441 pKa = 3.78 AGTNNNAGFTSTLAMTFVNGANEE464 pKa = 4.29 SGVTAFDD471 pKa = 3.58 PTALSSFFDD480 pKa = 3.33 NANYY484 pKa = 9.87 IGAVRR489 pKa = 11.84 DD490 pKa = 5.27 DD491 pKa = 3.9 ADD493 pKa = 2.82 TWYY496 pKa = 10.7 AGWTCNSATANFGTNTGDD514 pKa = 3.75 CTSLPIYY521 pKa = 10.61
Molecular weight: 53.7 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.724
IPC2_protein 3.846
IPC_protein 3.872
Toseland 3.643
ProMoST 4.037
Dawson 3.872
Bjellqvist 4.024
Wikipedia 3.808
Rodwell 3.694
Grimsley 3.554
Solomon 3.859
Lehninger 3.821
Nozaki 3.973
DTASelect 4.24
Thurlkill 3.694
EMBOSS 3.821
Sillero 3.999
Patrickios 0.896
IPC_peptide 3.859
IPC2_peptide 3.973
IPC2.peptide.svr19 3.871
Protein with the highest isoelectric point:
>tr|A0A0G3XC07|A0A0G3XC07_9SPHN Tail tape measure protein putative OS=Pelagerythrobacter marensis OX=543877 GN=AM2010_2062 PE=4 SV=1
MM1 pKa = 7.45 KK2 pKa = 9.61 RR3 pKa = 11.84 TFQPSNLVRR12 pKa = 11.84 KK13 pKa = 9.24 RR14 pKa = 11.84 RR15 pKa = 11.84 HH16 pKa = 4.93 GFFARR21 pKa = 11.84 KK22 pKa = 7.42 ATPGGRR28 pKa = 11.84 KK29 pKa = 8.05 VLRR32 pKa = 11.84 LRR34 pKa = 11.84 RR35 pKa = 11.84 RR36 pKa = 11.84 RR37 pKa = 11.84 GRR39 pKa = 11.84 KK40 pKa = 8.61 SLCAA44 pKa = 3.82
Molecular weight: 5.25 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.441
IPC2_protein 10.95
IPC_protein 12.544
Toseland 12.705
ProMoST 13.203
Dawson 12.705
Bjellqvist 12.705
Wikipedia 13.188
Rodwell 12.369
Grimsley 12.749
Solomon 13.203
Lehninger 13.1
Nozaki 12.705
DTASelect 12.705
Thurlkill 12.705
EMBOSS 13.203
Sillero 12.705
Patrickios 12.106
IPC_peptide 13.203
IPC2_peptide 12.193
IPC2.peptide.svr19 9.102
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2659
0
2659
874530
29
4338
328.9
35.55
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
13.257 ± 0.079
0.758 ± 0.013
6.34 ± 0.037
6.244 ± 0.05
3.56 ± 0.027
9.157 ± 0.065
1.898 ± 0.024
4.83 ± 0.034
2.617 ± 0.04
9.596 ± 0.065
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.369 ± 0.024
2.561 ± 0.048
5.225 ± 0.042
3.103 ± 0.022
7.542 ± 0.07
5.088 ± 0.044
5.187 ± 0.052
7.065 ± 0.036
1.39 ± 0.021
2.214 ± 0.033
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here