Amino acid dipepetide frequency for Streptomyces sp. CB00455

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	23.885AlaAla: 23.885 ± 0.187	1.106AlaCys: 1.106 ± 0.026	8.389AlaAsp: 8.389 ± 0.076	8.722AlaGlu: 8.722 ± 0.111	3.665AlaPhe: 3.665 ± 0.052	14.703AlaGly: 14.703 ± 0.112	3.051AlaHis: 3.051 ± 0.045	3.312AlaIle: 3.312 ± 0.044	3.02AlaLys: 3.02 ± 0.061	14.932AlaLeu: 14.932 ± 0.118	2.595AlaMet: 2.595 ± 0.038	1.919AlaAsn: 1.919 ± 0.036	8.026AlaPro: 8.026 ± 0.102	4.149AlaGln: 4.149 ± 0.058	10.589AlaArg: 10.589 ± 0.097	5.865AlaSer: 5.865 ± 0.064	6.944AlaThr: 6.944 ± 0.068	12.707AlaVal: 12.707 ± 0.091	1.934AlaTrp: 1.934 ± 0.032	2.797AlaTyr: 2.797 ± 0.042	0.0AlaXaa: 0.0 ± 0.0
Cys	1.205CysAla: 1.205 ± 0.028	0.081CysCys: 0.081 ± 0.007	0.511CysAsp: 0.511 ± 0.016	0.424CysGlu: 0.424 ± 0.015	0.216CysPhe: 0.216 ± 0.011	1.009CysGly: 1.009 ± 0.027	0.186CysHis: 0.186 ± 0.01	0.176CysIle: 0.176 ± 0.009	0.12CysLys: 0.12 ± 0.008	0.787CysLeu: 0.787 ± 0.021	0.132CysMet: 0.132 ± 0.008	0.153CysAsn: 0.153 ± 0.009	0.49CysPro: 0.49 ± 0.017	0.164CysGln: 0.164 ± 0.01	0.592CysArg: 0.592 ± 0.021	0.406CysSer: 0.406 ± 0.014	0.528CysThr: 0.528 ± 0.019	0.689CysVal: 0.689 ± 0.017	0.133CysTrp: 0.133 ± 0.009	0.148CysTyr: 0.148 ± 0.009	0.0CysXaa: 0.0 ± 0.0
Asp	7.529AspAla: 7.529 ± 0.073	0.465AspCys: 0.465 ± 0.016	3.05AspAsp: 3.05 ± 0.046	3.568AspGlu: 3.568 ± 0.046	1.619AspPhe: 1.619 ± 0.03	6.148AspGly: 6.148 ± 0.062	1.431AspHis: 1.431 ± 0.026	1.801AspIle: 1.801 ± 0.037	1.215AspLys: 1.215 ± 0.03	6.1AspLeu: 6.1 ± 0.056	0.819AspMet: 0.819 ± 0.02	0.935AspAsn: 0.935 ± 0.026	4.56AspPro: 4.56 ± 0.048	1.502AspGln: 1.502 ± 0.029	4.694AspArg: 4.694 ± 0.062	2.439AspSer: 2.439 ± 0.039	3.062AspThr: 3.062 ± 0.043	4.366AspVal: 4.366 ± 0.051	0.963AspTrp: 0.963 ± 0.024	1.081AspTyr: 1.081 ± 0.027	0.0AspXaa: 0.0 ± 0.0
Glu	7.623GluAla: 7.623 ± 0.09	0.355GluCys: 0.355 ± 0.014	2.777GluAsp: 2.777 ± 0.04	3.591GluGlu: 3.591 ± 0.049	1.438GluPhe: 1.438 ± 0.028	4.464GluGly: 4.464 ± 0.055	1.46GluHis: 1.46 ± 0.03	2.192GluIle: 2.192 ± 0.035	1.376GluLys: 1.376 ± 0.032	6.718GluLeu: 6.718 ± 0.071	0.886GluMet: 0.886 ± 0.02	1.001GluAsn: 1.001 ± 0.025	3.249GluPro: 3.249 ± 0.046	2.212GluGln: 2.212 ± 0.036	5.349GluArg: 5.349 ± 0.059	2.407GluSer: 2.407 ± 0.041	2.681GluThr: 2.681 ± 0.037	4.585GluVal: 4.585 ± 0.057	0.717GluTrp: 0.717 ± 0.019	1.082GluTyr: 1.082 ± 0.026	0.0GluXaa: 0.0 ± 0.0
Phe	3.816PheAla: 3.816 ± 0.047	0.295PheCys: 0.295 ± 0.012	1.925PheAsp: 1.925 ± 0.032	1.436PheGlu: 1.436 ± 0.027	0.895PhePhe: 0.895 ± 0.025	3.033PheGly: 3.033 ± 0.045	0.613PheHis: 0.613 ± 0.017	0.723PheIle: 0.723 ± 0.017	0.548PheLys: 0.548 ± 0.02	2.57PheLeu: 2.57 ± 0.044	0.418PheMet: 0.418 ± 0.012	0.556PheAsn: 0.556 ± 0.019	1.447PhePro: 1.447 ± 0.025	0.668PheGln: 0.668 ± 0.019	1.827PheArg: 1.827 ± 0.032	1.452PheSer: 1.452 ± 0.028	2.02PheThr: 2.02 ± 0.03	2.123PheVal: 2.123 ± 0.039	0.404PheTrp: 0.404 ± 0.016	0.584PheTyr: 0.584 ± 0.017	0.0PheXaa: 0.0 ± 0.0
Gly	12.289GlyAla: 12.289 ± 0.105	0.863GlyCys: 0.863 ± 0.021	5.016GlyAsp: 5.016 ± 0.055	5.191GlyGlu: 5.191 ± 0.054	2.962GlyPhe: 2.962 ± 0.041	9.433GlyGly: 9.433 ± 0.103	2.434GlyHis: 2.434 ± 0.036	3.587GlyIle: 3.587 ± 0.045	2.503GlyLys: 2.503 ± 0.046	9.642GlyLeu: 9.642 ± 0.081	2.026GlyMet: 2.026 ± 0.031	1.784GlyAsn: 1.784 ± 0.038	5.675GlyPro: 5.675 ± 0.074	2.745GlyGln: 2.745 ± 0.041	8.152GlyArg: 8.152 ± 0.077	5.57GlySer: 5.57 ± 0.059	6.084GlyThr: 6.084 ± 0.081	7.326GlyVal: 7.326 ± 0.07	1.688GlyTrp: 1.688 ± 0.033	2.317GlyTyr: 2.317 ± 0.044	0.0GlyXaa: 0.0 ± 0.0
His	2.766HisAla: 2.766 ± 0.039	0.228HisCys: 0.228 ± 0.012	1.292HisAsp: 1.292 ± 0.028	1.183HisGlu: 1.183 ± 0.023	0.648HisPhe: 0.648 ± 0.019	2.455HisGly: 2.455 ± 0.037	0.702HisHis: 0.702 ± 0.02	0.666HisIle: 0.666 ± 0.02	0.384HisLys: 0.384 ± 0.015	2.414HisLeu: 2.414 ± 0.038	0.37HisMet: 0.37 ± 0.013	0.376HisAsn: 0.376 ± 0.014	1.852HisPro: 1.852 ± 0.031	0.658HisGln: 0.658 ± 0.021	2.097HisArg: 2.097 ± 0.034	1.0HisSer: 1.0 ± 0.022	1.369HisThr: 1.369 ± 0.028	1.699HisVal: 1.699 ± 0.03	0.378HisTrp: 0.378 ± 0.015	0.485HisTyr: 0.485 ± 0.017	0.0HisXaa: 0.0 ± 0.0
Ile	4.748IleAla: 4.748 ± 0.057	0.304IleCys: 0.304 ± 0.012	2.143IleAsp: 2.143 ± 0.034	1.982IleGlu: 1.982 ± 0.036	0.677IlePhe: 0.677 ± 0.02	3.588IleGly: 3.588 ± 0.044	0.625IleHis: 0.625 ± 0.018	0.852IleIle: 0.852 ± 0.025	0.745IleLys: 0.745 ± 0.024	2.384IleLeu: 2.384 ± 0.037	0.438IleMet: 0.438 ± 0.016	0.693IleAsn: 0.693 ± 0.018	1.707IlePro: 1.707 ± 0.029	0.747IleGln: 0.747 ± 0.022	2.231IleArg: 2.231 ± 0.035	1.636IleSer: 1.636 ± 0.027	2.178IleThr: 2.178 ± 0.035	2.574IleVal: 2.574 ± 0.034	0.352IleTrp: 0.352 ± 0.015	0.491IleTyr: 0.491 ± 0.017	0.0IleXaa: 0.0 ± 0.0
Lys	3.086LysAla: 3.086 ± 0.053	0.121LysCys: 0.121 ± 0.009	1.365LysAsp: 1.365 ± 0.038	1.179LysGlu: 1.179 ± 0.027	0.464LysPhe: 0.464 ± 0.016	1.893LysGly: 1.893 ± 0.039	0.399LysHis: 0.399 ± 0.015	0.899LysIle: 0.899 ± 0.026	0.8LysLys: 0.8 ± 0.031	1.981LysLeu: 1.981 ± 0.038	0.425LysMet: 0.425 ± 0.014	0.508LysAsn: 0.508 ± 0.019	1.345LysPro: 1.345 ± 0.031	0.687LysGln: 0.687 ± 0.018	1.39LysArg: 1.39 ± 0.029	1.122LysSer: 1.122 ± 0.031	1.336LysThr: 1.336 ± 0.035	1.9LysVal: 1.9 ± 0.037	0.258LysTrp: 0.258 ± 0.013	0.46LysTyr: 0.46 ± 0.016	0.0LysXaa: 0.0 ± 0.0
Leu	15.609LeuAla: 15.609 ± 0.107	0.908LeuCys: 0.908 ± 0.023	6.484LeuAsp: 6.484 ± 0.062	4.859LeuGlu: 4.859 ± 0.059	2.618LeuPhe: 2.618 ± 0.047	9.404LeuGly: 9.404 ± 0.084	2.313LeuHis: 2.313 ± 0.036	3.181LeuIle: 3.181 ± 0.048	1.901LeuLys: 1.901 ± 0.034	11.394LeuLeu: 11.394 ± 0.112	1.644LeuMet: 1.644 ± 0.033	1.601LeuAsn: 1.601 ± 0.03	6.572LeuPro: 6.572 ± 0.062	2.255LeuGln: 2.255 ± 0.036	8.617LeuArg: 8.617 ± 0.085	5.178LeuSer: 5.178 ± 0.048	6.653LeuThr: 6.653 ± 0.07	8.716LeuVal: 8.716 ± 0.077	1.285LeuTrp: 1.285 ± 0.029	1.859LeuTyr: 1.859 ± 0.031	0.001LeuXaa: 0.001 ± 0.0
Met	2.409MetAla: 2.409 ± 0.037	0.14MetCys: 0.14 ± 0.008	0.932MetAsp: 0.932 ± 0.02	0.784MetGlu: 0.784 ± 0.022	0.448MetPhe: 0.448 ± 0.016	1.365MetGly: 1.365 ± 0.03	0.35MetHis: 0.35 ± 0.014	0.674MetIle: 0.674 ± 0.021	0.444MetLys: 0.444 ± 0.014	1.714MetLeu: 1.714 ± 0.03	0.305MetMet: 0.305 ± 0.013	0.451MetAsn: 0.451 ± 0.014	1.178MetPro: 1.178 ± 0.026	0.439MetGln: 0.439 ± 0.016	1.448MetArg: 1.448 ± 0.028	1.334MetSer: 1.334 ± 0.024	1.507MetThr: 1.507 ± 0.03	1.256MetVal: 1.256 ± 0.026	0.211MetTrp: 0.211 ± 0.011	0.307MetTyr: 0.307 ± 0.014	0.0MetXaa: 0.0 ± 0.0
Asn	2.215AsnAla: 2.215 ± 0.035	0.155AsnCys: 0.155 ± 0.008	0.915AsnAsp: 0.915 ± 0.025	0.819AsnGlu: 0.819 ± 0.02	0.492AsnPhe: 0.492 ± 0.015	1.805AsnGly: 1.805 ± 0.038	0.377AsnHis: 0.377 ± 0.015	0.616AsnIle: 0.616 ± 0.017	0.415AsnLys: 0.415 ± 0.017	1.637AsnLeu: 1.637 ± 0.029	0.305AsnMet: 0.305 ± 0.013	0.429AsnAsn: 0.429 ± 0.022	1.344AsnPro: 1.344 ± 0.03	0.504AsnGln: 0.504 ± 0.019	1.254AsnArg: 1.254 ± 0.024	0.875AsnSer: 0.875 ± 0.025	1.093AsnThr: 1.093 ± 0.026	1.331AsnVal: 1.331 ± 0.025	0.288AsnTrp: 0.288 ± 0.013	0.38AsnTyr: 0.38 ± 0.015	0.0AsnXaa: 0.0 ± 0.0
Pro	10.056ProAla: 10.056 ± 0.112	0.345ProCys: 0.345 ± 0.013	4.256ProAsp: 4.256 ± 0.05	4.129ProGlu: 4.129 ± 0.054	1.554ProPhe: 1.554 ± 0.03	7.292ProGly: 7.292 ± 0.089	1.414ProHis: 1.414 ± 0.027	1.14ProIle: 1.14 ± 0.026	1.143ProLys: 1.143 ± 0.029	5.498ProLeu: 5.498 ± 0.061	1.013ProMet: 1.013 ± 0.02	0.846ProAsn: 0.846 ± 0.021	3.65ProPro: 3.65 ± 0.062	2.078ProGln: 2.078 ± 0.041	4.009ProArg: 4.009 ± 0.049	3.074ProSer: 3.074 ± 0.043	2.948ProThr: 2.948 ± 0.04	5.703ProVal: 5.703 ± 0.062	0.922ProTrp: 0.922 ± 0.024	1.406ProTyr: 1.406 ± 0.028	0.001ProXaa: 0.001 ± 0.001
Gln	3.921GlnAla: 3.921 ± 0.053	0.184GlnCys: 0.184 ± 0.009	1.517GlnAsp: 1.517 ± 0.028	1.574GlnGlu: 1.574 ± 0.035	0.688GlnPhe: 0.688 ± 0.02	2.464GlnGly: 2.464 ± 0.037	0.644GlnHis: 0.644 ± 0.019	1.097GlnIle: 1.097 ± 0.025	0.646GlnLys: 0.646 ± 0.021	2.99GlnLeu: 2.99 ± 0.037	0.515GlnMet: 0.515 ± 0.019	0.502GlnAsn: 0.502 ± 0.019	1.766GlnPro: 1.766 ± 0.042	1.258GlnGln: 1.258 ± 0.043	2.25GlnArg: 2.25 ± 0.036	1.31GlnSer: 1.31 ± 0.024	1.356GlnThr: 1.356 ± 0.027	2.366GlnVal: 2.366 ± 0.035	0.476GlnTrp: 0.476 ± 0.016	0.626GlnTyr: 0.626 ± 0.018	0.0GlnXaa: 0.0 ± 0.0
Arg	10.1ArgAla: 10.1 ± 0.108	0.58ArgCys: 0.58 ± 0.019	4.064ArgAsp: 4.064 ± 0.047	4.793ArgGlu: 4.793 ± 0.054	2.338ArgPhe: 2.338 ± 0.033	5.985ArgGly: 5.985 ± 0.061	2.121ArgHis: 2.121 ± 0.035	3.342ArgIle: 3.342 ± 0.042	1.606ArgLys: 1.606 ± 0.036	8.684ArgLeu: 8.684 ± 0.091	1.775ArgMet: 1.775 ± 0.032	1.269ArgAsn: 1.269 ± 0.03	5.042ArgPro: 5.042 ± 0.07	2.198ArgGln: 2.198 ± 0.032	7.52ArgArg: 7.52 ± 0.077	4.063ArgSer: 4.063 ± 0.05	5.405ArgThr: 5.405 ± 0.059	5.598ArgVal: 5.598 ± 0.057	1.399ArgTrp: 1.399 ± 0.027	1.77ArgTyr: 1.77 ± 0.028	0.001ArgXaa: 0.001 ± 0.001
Ser	6.955SerAla: 6.955 ± 0.069	0.443SerCys: 0.443 ± 0.016	2.426SerAsp: 2.426 ± 0.04	2.32SerGlu: 2.32 ± 0.03	1.539SerPhe: 1.539 ± 0.026	5.821SerGly: 5.821 ± 0.06	0.979SerHis: 0.979 ± 0.02	1.351SerIle: 1.351 ± 0.026	1.002SerLys: 1.002 ± 0.026	4.772SerLeu: 4.772 ± 0.052	1.058SerMet: 1.058 ± 0.024	0.824SerAsn: 0.824 ± 0.024	3.218SerPro: 3.218 ± 0.047	1.26SerGln: 1.26 ± 0.025	3.586SerArg: 3.586 ± 0.04	2.684SerSer: 2.684 ± 0.044	2.833SerThr: 2.833 ± 0.045	4.215SerVal: 4.215 ± 0.047	0.903SerTrp: 0.903 ± 0.023	1.184SerTyr: 1.184 ± 0.024	0.0SerXaa: 0.0 ± 0.0
Thr	8.973ThrAla: 8.973 ± 0.077	0.442ThrCys: 0.442 ± 0.014	3.335ThrAsp: 3.335 ± 0.04	3.004ThrGlu: 3.004 ± 0.039	1.538ThrPhe: 1.538 ± 0.033	6.808ThrGly: 6.808 ± 0.066	1.189ThrHis: 1.189 ± 0.026	1.581ThrIle: 1.581 ± 0.035	1.149ThrLys: 1.149 ± 0.03	5.535ThrLeu: 5.535 ± 0.056	0.953ThrMet: 0.953 ± 0.022	0.928ThrAsn: 0.928 ± 0.024	4.103ThrPro: 4.103 ± 0.059	1.33ThrGln: 1.33 ± 0.03	3.785ThrArg: 3.785 ± 0.041	2.882ThrSer: 2.882 ± 0.038	3.622ThrThr: 3.622 ± 0.062	5.788ThrVal: 5.788 ± 0.067	0.875ThrTrp: 0.875 ± 0.023	1.3ThrTyr: 1.3 ± 0.035	0.0ThrXaa: 0.0 ± 0.0
Val	10.566ValAla: 10.566 ± 0.091	0.795ValCys: 0.795 ± 0.02	4.72ValAsp: 4.72 ± 0.049	4.571ValGlu: 4.571 ± 0.052	2.411ValPhe: 2.411 ± 0.034	6.239ValGly: 6.239 ± 0.064	1.999ValHis: 1.999 ± 0.032	2.869ValIle: 2.869 ± 0.04	1.797ValLys: 1.797 ± 0.04	9.507ValLeu: 9.507 ± 0.091	1.448ValMet: 1.448 ± 0.031	1.681ValAsn: 1.681 ± 0.035	5.399ValPro: 5.399 ± 0.059	2.088ValGln: 2.088 ± 0.032	7.176ValArg: 7.176 ± 0.069	4.233ValSer: 4.233 ± 0.053	5.321ValThr: 5.321 ± 0.056	7.784ValVal: 7.784 ± 0.079	1.124ValTrp: 1.124 ± 0.022	1.566ValTyr: 1.566 ± 0.028	0.0ValXaa: 0.0 ± 0.0
Trp	1.671TrpAla: 1.671 ± 0.033	0.143TrpCys: 0.143 ± 0.008	0.826TrpAsp: 0.826 ± 0.022	0.731TrpGlu: 0.731 ± 0.019	0.501TrpPhe: 0.501 ± 0.016	1.046TrpGly: 1.046 ± 0.023	0.355TrpHis: 0.355 ± 0.013	0.571TrpIle: 0.571 ± 0.017	0.394TrpLys: 0.394 ± 0.016	1.73TrpLeu: 1.73 ± 0.029	0.309TrpMet: 0.309 ± 0.013	0.393TrpAsn: 0.393 ± 0.015	0.789TrpPro: 0.789 ± 0.023	0.626TrpGln: 0.626 ± 0.019	1.276TrpArg: 1.276 ± 0.026	0.935TrpSer: 0.935 ± 0.023	1.044TrpThr: 1.044 ± 0.027	0.949TrpVal: 0.949 ± 0.022	0.335TrpTrp: 0.335 ± 0.015	0.35TrpTyr: 0.35 ± 0.014	0.001TrpXaa: 0.001 ± 0.001
Tyr	2.89TyrAla: 2.89 ± 0.035	0.198TyrCys: 0.198 ± 0.01	1.403TyrAsp: 1.403 ± 0.032	1.304TyrGlu: 1.304 ± 0.029	0.643TyrPhe: 0.643 ± 0.02	2.333TyrGly: 2.333 ± 0.039	0.378TyrHis: 0.378 ± 0.012	0.463TyrIle: 0.463 ± 0.015	0.412TyrLys: 0.412 ± 0.017	2.063TyrLeu: 2.063 ± 0.035	0.261TyrMet: 0.261 ± 0.011	0.4TyrAsn: 0.4 ± 0.016	1.059TyrPro: 1.059 ± 0.024	0.582TyrGln: 0.582 ± 0.017	1.81TyrArg: 1.81 ± 0.029	0.91TyrSer: 0.91 ± 0.023	1.134TyrThr: 1.134 ± 0.024	1.621TyrVal: 1.621 ± 0.027	0.331TyrTrp: 0.331 ± 0.013	0.426TyrTyr: 0.426 ± 0.017	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.001XaaThr: 0.001 ± 0.001	0.001XaaVal: 0.001 ± 0.001	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 6119 proteins (1953217 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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