Lachnospiraceae bacterium C7

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Clostridia; Eubacteriales; Lachnospiraceae; unclassified Lachnospiraceae

Average proteome isoelectric point is 6.3

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2493 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1I2RQG0|A0A1I2RQG0_9FIRM Flavorubredoxin OS=Lachnospiraceae bacterium C7 OX=1792306 GN=SAMN04487761_11830 PE=3 SV=1
MM1 pKa = 8.19IEE3 pKa = 4.64FKK5 pKa = 11.04YY6 pKa = 9.74DD7 pKa = 3.29TQLLIEE13 pKa = 4.36GHH15 pKa = 7.06DD16 pKa = 4.01LDD18 pKa = 4.72EE19 pKa = 5.5DD20 pKa = 4.68AINDD24 pKa = 3.67YY25 pKa = 8.87FTEE28 pKa = 4.12NFKK31 pKa = 11.06GDD33 pKa = 3.68CLLAVGDD40 pKa = 4.4DD41 pKa = 3.96EE42 pKa = 5.76LIKK45 pKa = 10.37IHH47 pKa = 5.82YY48 pKa = 7.07HH49 pKa = 4.99TNEE52 pKa = 3.45PWLVLEE58 pKa = 4.59YY59 pKa = 10.35CASLGEE65 pKa = 4.29IYY67 pKa = 10.46DD68 pKa = 3.76IVVEE72 pKa = 4.74DD73 pKa = 4.1MDD75 pKa = 3.77RR76 pKa = 11.84QARR79 pKa = 11.84GLQGG83 pKa = 3.1

Molecular weight: 9.63 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1I2UJX8|A0A1I2UJX8_9FIRM Putative peptidoglycan binding domain-containing protein OS=Lachnospiraceae bacterium C7 OX=1792306 GN=SAMN04487761_15011 PE=4 SV=1
MM1 pKa = 7.67KK2 pKa = 8.72MTFQPKK8 pKa = 8.95KK9 pKa = 7.58RR10 pKa = 11.84QRR12 pKa = 11.84SKK14 pKa = 9.07VHH16 pKa = 5.95GFRR19 pKa = 11.84KK20 pKa = 9.97RR21 pKa = 11.84MSTAGGRR28 pKa = 11.84KK29 pKa = 8.71VLAARR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 10.1GRR39 pKa = 11.84KK40 pKa = 8.83KK41 pKa = 10.63LSAA44 pKa = 3.95

Molecular weight: 5.07 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2493

0

2493

815008

8

1558

326.9

36.88

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.523 ± 0.042

1.297 ± 0.016

6.079 ± 0.041

7.532 ± 0.057

4.233 ± 0.035

6.374 ± 0.046

1.55 ± 0.018

8.229 ± 0.052

8.865 ± 0.047

8.234 ± 0.048

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.91 ± 0.022

5.67 ± 0.037

2.79 ± 0.023

2.903 ± 0.03

3.518 ± 0.033

6.061 ± 0.042

5.298 ± 0.037

6.909 ± 0.04

0.728 ± 0.014

4.297 ± 0.042
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski