Enterococcus phage EF62phi

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Podoviridae; unclassified Podoviridae

Average proteome isoelectric point is 6.08

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 48 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|F0PIH6|F0PIH6_9CAUD Uncharacterized protein OS=Enterococcus phage EF62phi OX=977801 GN=EF62_phi0011 PE=4 SV=1
MM1 pKa = 7.57KK2 pKa = 9.9PGKK5 pKa = 10.41LSFLDD10 pKa = 3.43LALIIEE16 pKa = 5.24GIYY19 pKa = 10.7YY20 pKa = 8.67GTEE23 pKa = 3.69DD24 pKa = 3.84VDD26 pKa = 3.24ICEE29 pKa = 4.57IEE31 pKa = 4.72DD32 pKa = 3.73LLEE35 pKa = 4.04YY36 pKa = 10.85LHH38 pKa = 7.46DD39 pKa = 4.54NGHH42 pKa = 5.71MVAVLNTFEE51 pKa = 4.79EE52 pKa = 4.72EE53 pKa = 4.3VEE55 pKa = 4.08

Molecular weight: 6.29 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|F0PIL3|F0PIL3_9CAUD Uncharacterized protein OS=Enterococcus phage EF62phi OX=977801 GN=EF62_phi0048 PE=4 SV=1
MM1 pKa = 7.1FAISSVVSTLRR12 pKa = 11.84RR13 pKa = 11.84KK14 pKa = 10.03RR15 pKa = 11.84LGLCLFPRR23 pKa = 11.84VILFLYY29 pKa = 10.37SLSFLIGNPLSLPQAEE45 pKa = 3.99RR46 pKa = 11.84TIKK49 pKa = 10.68PEE51 pKa = 3.71GLVCHH56 pKa = 6.69AAGIALGYY64 pKa = 10.48NRR66 pKa = 11.84TASLSINHH74 pKa = 5.66YY75 pKa = 10.47FII77 pKa = 6.31

Molecular weight: 8.54 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

48

0

48

9264

43

824

193.0

22.19

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.041 ± 0.264

0.788 ± 0.117

6.142 ± 0.244

8.56 ± 0.569

4.674 ± 0.24

5.764 ± 0.405

1.306 ± 0.116

7.222 ± 0.351

8.733 ± 0.363

8.236 ± 0.395

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.472 ± 0.134

7.297 ± 0.412

3.433 ± 0.368

3.886 ± 0.248

3.713 ± 0.288

5.592 ± 0.307

5.624 ± 0.283

5.602 ± 0.296

1.144 ± 0.203

4.771 ± 0.267
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski