Arthrobacter phage vB_ArS-ArV2

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.57

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 68 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|V5R901|V5R901_9CAUD Uncharacterized protein OS=Arthrobacter phage vB_ArS-ArV2 OX=1414742 GN=ArV2_gp27 PE=4 SV=1
MM1 pKa = 7.48WALFLCPEE9 pKa = 5.0GDD11 pKa = 3.3THH13 pKa = 6.48MADD16 pKa = 3.48YY17 pKa = 9.25PYY19 pKa = 10.61DD20 pKa = 3.85GQLIADD26 pKa = 4.12PVTFQRR32 pKa = 11.84ATNAQITVYY41 pKa = 10.66DD42 pKa = 3.94AADD45 pKa = 3.46TGNTTKK51 pKa = 10.82LALKK55 pKa = 8.66DD56 pKa = 3.64TSGLPLANPLTSTADD71 pKa = 3.16AFTVPFYY78 pKa = 11.22APSQDD83 pKa = 2.86IKK85 pKa = 11.34LVGAGLTVFVSSAKK99 pKa = 10.2GMRR102 pKa = 11.84DD103 pKa = 2.96AAAAAAAAAQAAASNAATEE122 pKa = 4.15AAAGVASVVSAAASAKK138 pKa = 9.02TAAEE142 pKa = 3.93TAAASAASAAALVNAPADD160 pKa = 3.75AAIEE164 pKa = 3.9AAILGAGTKK173 pKa = 9.09TKK175 pKa = 10.6AALSATYY182 pKa = 10.75VVFRR186 pKa = 11.84NHH188 pKa = 7.37DD189 pKa = 3.94GTPVAAPKK197 pKa = 10.45VVAITLTADD206 pKa = 3.4GADD209 pKa = 3.34IDD211 pKa = 4.82NIAVYY216 pKa = 10.75NSLEE220 pKa = 4.23EE221 pKa = 4.23VGSS224 pKa = 3.72

Molecular weight: 22.14 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|V5RAE4|V5RAE4_9CAUD DNA N-6-adenine-methyltransferase OS=Arthrobacter phage vB_ArS-ArV2 OX=1414742 GN=ArV2_gp64 PE=4 SV=1
MM1 pKa = 7.37AHH3 pKa = 7.22RR4 pKa = 11.84RR5 pKa = 11.84LQPPPRR11 pKa = 11.84PNSPTSHH18 pKa = 6.73PQRR21 pKa = 11.84HH22 pKa = 5.04EE23 pKa = 4.17RR24 pKa = 11.84KK25 pKa = 8.03EE26 pKa = 4.24TAQMTYY32 pKa = 10.28VRR34 pKa = 11.84GKK36 pKa = 10.07AHH38 pKa = 6.87PQTIPVLQATISDD51 pKa = 4.19LLKK54 pKa = 10.61EE55 pKa = 4.06RR56 pKa = 11.84ASLKK60 pKa = 9.66RR61 pKa = 11.84TIEE64 pKa = 4.07ALSSQVQALNAVPANFRR81 pKa = 11.84RR82 pKa = 11.84AEE84 pKa = 4.26PVCGTYY90 pKa = 10.04SGYY93 pKa = 10.27QKK95 pKa = 10.65HH96 pKa = 6.3IRR98 pKa = 11.84TKK100 pKa = 8.36EE101 pKa = 4.38TPCFPCKK108 pKa = 9.91AARR111 pKa = 11.84AEE113 pKa = 3.93YY114 pKa = 8.52TRR116 pKa = 11.84NYY118 pKa = 10.21RR119 pKa = 11.84KK120 pKa = 10.18LKK122 pKa = 10.62AVAA125 pKa = 3.95

Molecular weight: 14.2 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

68

0

68

12048

42

1208

177.2

19.3

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.06 ± 0.448

1.104 ± 0.199

5.852 ± 0.236

5.76 ± 0.406

3.196 ± 0.294

8.599 ± 0.559

2.307 ± 0.175

4.407 ± 0.374

4.299 ± 0.291

7.503 ± 0.271

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.216 ± 0.14

2.88 ± 0.232

5.835 ± 0.341

3.619 ± 0.205

6.781 ± 0.545

5.86 ± 0.23

6.441 ± 0.281

6.906 ± 0.356

2.083 ± 0.187

2.291 ± 0.172
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski