Marinobacter daqiaonensis
Average proteome isoelectric point is 6.02
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3504 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A1I6H1L6|A0A1I6H1L6_9ALTE Cold-shock DNA-binding protein family OS=Marinobacter daqiaonensis OX=650891 GN=SAMN05216203_0737 PE=4 SV=1
MM1 pKa = 7.61 KK2 pKa = 10.05 KK3 pKa = 10.59 LPIAVTASLLAFAPVASADD22 pKa = 3.54 VLGFGASVGYY32 pKa = 9.37 WDD34 pKa = 4.81 SDD36 pKa = 3.65 LSGEE40 pKa = 4.09 ASRR43 pKa = 11.84 GNDD46 pKa = 3.11 QVDD49 pKa = 3.54 VEE51 pKa = 4.37 NDD53 pKa = 3.32 LNLSNNDD60 pKa = 3.84 EE61 pKa = 4.63 INLSAYY67 pKa = 10.26 LEE69 pKa = 4.33 HH70 pKa = 7.8 PIPLLPNVRR79 pKa = 11.84 LSYY82 pKa = 11.04 VRR84 pKa = 11.84 IDD86 pKa = 3.35 QEE88 pKa = 4.54 GRR90 pKa = 11.84 GQLSTSFDD98 pKa = 3.7 VLPSGVNVSSQLDD111 pKa = 3.42 WTQADD116 pKa = 3.5 LTLYY120 pKa = 11.24 YY121 pKa = 10.3 EE122 pKa = 5.11 VLDD125 pKa = 3.39 NWVNLDD131 pKa = 3.71 LGLTARR137 pKa = 11.84 NLDD140 pKa = 3.51 AEE142 pKa = 4.54 LVVQEE147 pKa = 4.47 VNSTAVSRR155 pKa = 11.84 TEE157 pKa = 3.65 VDD159 pKa = 2.97 AVLPMLYY166 pKa = 10.24 AAARR170 pKa = 11.84 FDD172 pKa = 4.42 LPLTGLSVGAHH183 pKa = 5.89 GNVIGYY189 pKa = 9.85 DD190 pKa = 3.28 GDD192 pKa = 4.09 SIYY195 pKa = 10.85 DD196 pKa = 3.44 YY197 pKa = 11.15 DD198 pKa = 5.74 AYY200 pKa = 10.78 AQYY203 pKa = 11.15 QFVGVQLRR211 pKa = 11.84 AGYY214 pKa = 10.03 RR215 pKa = 11.84 QMTVDD220 pKa = 4.73 YY221 pKa = 10.63 EE222 pKa = 4.93 DD223 pKa = 4.7 GDD225 pKa = 4.05 DD226 pKa = 5.06 RR227 pKa = 11.84 LDD229 pKa = 3.43 VDD231 pKa = 3.23 IAGPFVSAGFSFF243 pKa = 4.27
Molecular weight: 26.42 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.718
IPC2_protein 3.783
IPC_protein 3.808
Toseland 3.579
ProMoST 3.986
Dawson 3.808
Bjellqvist 3.973
Wikipedia 3.77
Rodwell 3.63
Grimsley 3.49
Solomon 3.808
Lehninger 3.77
Nozaki 3.923
DTASelect 4.202
Thurlkill 3.643
EMBOSS 3.783
Sillero 3.935
Patrickios 1.901
IPC_peptide 3.795
IPC2_peptide 3.91
IPC2.peptide.svr19 3.831
Protein with the highest isoelectric point:
>tr|A0A1I6IGQ3|A0A1I6IGQ3_9ALTE Cytochrome c oxidase assembly protein subunit 15 OS=Marinobacter daqiaonensis OX=650891 GN=SAMN05216203_2277 PE=4 SV=1
MM1 pKa = 7.45 KK2 pKa = 9.51 RR3 pKa = 11.84 TFQPSVLKK11 pKa = 10.34 RR12 pKa = 11.84 KK13 pKa = 9.1 RR14 pKa = 11.84 VHH16 pKa = 6.26 GFRR19 pKa = 11.84 ARR21 pKa = 11.84 MATANGRR28 pKa = 11.84 KK29 pKa = 8.91 VLSRR33 pKa = 11.84 RR34 pKa = 11.84 RR35 pKa = 11.84 AKK37 pKa = 10.44 GRR39 pKa = 11.84 ARR41 pKa = 11.84 LSAA44 pKa = 3.91
Molecular weight: 5.11 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.523
IPC2_protein 11.242
IPC_protein 12.837
Toseland 13.013
ProMoST 13.495
Dawson 13.013
Bjellqvist 12.998
Wikipedia 13.481
Rodwell 12.676
Grimsley 13.042
Solomon 13.495
Lehninger 13.408
Nozaki 13.013
DTASelect 12.998
Thurlkill 13.013
EMBOSS 13.51
Sillero 13.013
Patrickios 12.398
IPC_peptide 13.51
IPC2_peptide 12.486
IPC2.peptide.svr19 9.177
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3504
0
3504
1168533
29
2544
333.5
36.8
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
9.852 ± 0.036
0.891 ± 0.012
5.84 ± 0.029
6.784 ± 0.045
3.663 ± 0.029
8.12 ± 0.035
2.238 ± 0.021
5.083 ± 0.028
3.164 ± 0.032
10.774 ± 0.048
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.623 ± 0.019
2.987 ± 0.022
4.847 ± 0.024
3.859 ± 0.024
7.125 ± 0.042
5.754 ± 0.03
5.145 ± 0.024
7.351 ± 0.032
1.399 ± 0.02
2.501 ± 0.02
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here