Beihai picorna-like virus 119
Average proteome isoelectric point is 5.41
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A1L3KHD1|A0A1L3KHD1_9VIRU Calici_coat domain-containing protein OS=Beihai picorna-like virus 119 OX=1922548 PE=4 SV=1
MM1 pKa = 7.16 ATSDD5 pKa = 4.47 GLTEE9 pKa = 4.67 DD10 pKa = 4.62 LSSNSPPTGGVTSEE24 pKa = 4.26 EE25 pKa = 4.16 VQPDD29 pKa = 3.83 LTADD33 pKa = 3.52 FQKK36 pKa = 11.22 NVFTHH41 pKa = 5.22 NTIDD45 pKa = 3.57 SYY47 pKa = 11.76 LVGHH51 pKa = 6.82 PFVVGHH57 pKa = 6.15 FSWNTNQVEE66 pKa = 4.57 GTVLHH71 pKa = 6.08 SLKK74 pKa = 10.32 LHH76 pKa = 6.69 PSNFPPEE83 pKa = 3.95 LRR85 pKa = 11.84 ALVMMYY91 pKa = 9.27 EE92 pKa = 4.29 YY93 pKa = 8.21 WTGSVDD99 pKa = 3.99 VVLTIPGTGLHH110 pKa = 6.36 GGKK113 pKa = 10.36 LMVYY117 pKa = 9.74 SMPPGRR123 pKa = 11.84 PIEE126 pKa = 4.27 EE127 pKa = 4.11 GVIEE131 pKa = 3.98 QSIYY135 pKa = 10.6 PWISLDD141 pKa = 3.63 AKK143 pKa = 9.36 EE144 pKa = 4.1 QSSLAFQVQDD154 pKa = 3.13 QCRR157 pKa = 11.84 VKK159 pKa = 9.44 WHH161 pKa = 4.76 QTSTFEE167 pKa = 4.11 EE168 pKa = 4.16 EE169 pKa = 4.54 DD170 pKa = 3.47 YY171 pKa = 11.6 GRR173 pKa = 11.84 TIVMTVLSKK182 pKa = 10.94 LIATTPQASDD192 pKa = 3.21 VRR194 pKa = 11.84 VVVSFAIRR202 pKa = 11.84 SDD204 pKa = 3.66 FRR206 pKa = 11.84 VSTLKK211 pKa = 10.55 PMPFTKK217 pKa = 10.29 PVATAFLRR225 pKa = 11.84 DD226 pKa = 3.6 FDD228 pKa = 5.49 FSDD231 pKa = 3.7 ALDD234 pKa = 4.22 RR235 pKa = 11.84 LHH237 pKa = 7.28 PMIDD241 pKa = 3.53 FEE243 pKa = 5.07 LNEE246 pKa = 4.18 IRR248 pKa = 11.84 VSPNTTAGAEE258 pKa = 4.24 FADD261 pKa = 3.71 TTVNFKK267 pKa = 10.81 GINPKK272 pKa = 9.93 QNHH275 pKa = 4.46 QSYY278 pKa = 10.84 RR279 pKa = 11.84 DD280 pKa = 3.89 AQLHH284 pKa = 5.0 GALWIDD290 pKa = 3.35 RR291 pKa = 11.84 GEE293 pKa = 4.05 EE294 pKa = 3.26 TGYY297 pKa = 11.47 ARR299 pKa = 11.84 IHH301 pKa = 6.47 KK302 pKa = 10.22 EE303 pKa = 4.01 SVPDD307 pKa = 3.65 KK308 pKa = 11.57 AEE310 pKa = 3.95 GDD312 pKa = 3.44 QYY314 pKa = 11.49 RR315 pKa = 11.84 IFFVGLKK322 pKa = 8.38 MVRR325 pKa = 11.84 SNGAGDD331 pKa = 3.52 TDD333 pKa = 3.06 ISFNHH338 pKa = 6.41 PVGGAQLTINVPGSEE353 pKa = 3.84 RR354 pKa = 11.84 AALLSIDD361 pKa = 4.33 APRR364 pKa = 11.84 HH365 pKa = 5.13 GNWGAIPDD373 pKa = 4.16 GPVAINDD380 pKa = 3.7 AGEE383 pKa = 4.22 SMVYY387 pKa = 10.44 FSFTDD392 pKa = 3.15 DD393 pKa = 4.05 HH394 pKa = 6.4 EE395 pKa = 4.77 AQGVALPFLVSEE407 pKa = 5.41 AILNGAFEE415 pKa = 4.59 SLPDD419 pKa = 3.96 DD420 pKa = 4.21 VSVVWDD426 pKa = 3.85 VKK428 pKa = 11.03 HH429 pKa = 6.15 KK430 pKa = 10.12 VTGAQYY436 pKa = 11.12 GKK438 pKa = 10.04 IRR440 pKa = 11.84 LWPSGFFTTGRR451 pKa = 11.84 VATWTVIPLEE461 pKa = 4.06 NLRR464 pKa = 11.84 FTNPVTWPIRR474 pKa = 11.84 SALPFNDD481 pKa = 3.65 SGAQAALANMQLAQAQRR498 pKa = 11.84 QIKK501 pKa = 10.25 DD502 pKa = 3.08 LEE504 pKa = 4.12 QRR506 pKa = 11.84 VNALAIAVDD515 pKa = 4.08 DD516 pKa = 4.76 GEE518 pKa = 4.17
Molecular weight: 57.06 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.937
IPC2_protein 4.902
IPC_protein 4.876
Toseland 4.813
ProMoST 4.991
Dawson 4.889
Bjellqvist 5.029
Wikipedia 4.774
Rodwell 4.787
Grimsley 4.736
Solomon 4.889
Lehninger 4.838
Nozaki 5.003
DTASelect 5.194
Thurlkill 4.825
EMBOSS 4.825
Sillero 5.067
Patrickios 4.113
IPC_peptide 4.889
IPC2_peptide 5.067
IPC2.peptide.svr19 4.962
Protein with the highest isoelectric point:
>tr|A0A1L3KHD1|A0A1L3KHD1_9VIRU Calici_coat domain-containing protein OS=Beihai picorna-like virus 119 OX=1922548 PE=4 SV=1
MM1 pKa = 7.78 ALPPEE6 pKa = 4.37 YY7 pKa = 10.26 KK8 pKa = 9.67 PCTINRR14 pKa = 11.84 TDD16 pKa = 3.11 HH17 pKa = 5.33 TRR19 pKa = 11.84 FRR21 pKa = 11.84 TMLDD25 pKa = 3.03 HH26 pKa = 7.67 PEE28 pKa = 5.63 DD29 pKa = 4.0 FFQDD33 pKa = 4.0 CLPEE37 pKa = 4.14 PLQPLPLSFVRR48 pKa = 11.84 GATQRR53 pKa = 11.84 VAPHH57 pKa = 6.09 SSKK60 pKa = 9.16 FTKK63 pKa = 9.76 MLPKK67 pKa = 10.03 QFKK70 pKa = 10.26 QILKK74 pKa = 5.55 TTCNEE79 pKa = 3.7 RR80 pKa = 11.84 TGRR83 pKa = 11.84 TIHH86 pKa = 6.61 LVGCIYY92 pKa = 10.07 QVPHH96 pKa = 6.49 HH97 pKa = 6.69 VLTGQTLQPEE107 pKa = 5.51 DD108 pKa = 4.33 IPNLCPDD115 pKa = 4.25 HH116 pKa = 7.84 DD117 pKa = 5.24 SIQYY121 pKa = 10.53 LYY123 pKa = 9.62 TRR125 pKa = 11.84 NGRR128 pKa = 11.84 LMRR131 pKa = 11.84 CYY133 pKa = 9.18 HH134 pKa = 6.04 TNSKK138 pKa = 10.72 RR139 pKa = 11.84 NIARR143 pKa = 11.84 YY144 pKa = 8.49 YY145 pKa = 8.94 WDD147 pKa = 4.01 RR148 pKa = 11.84 FDD150 pKa = 4.27 TFTDD154 pKa = 3.33 QAQQIFIHH162 pKa = 6.79 RR163 pKa = 11.84 EE164 pKa = 3.57 DD165 pKa = 3.7 EE166 pKa = 4.9 GYY168 pKa = 10.82 HH169 pKa = 4.75 SAEE172 pKa = 3.89 YY173 pKa = 10.49 NRR175 pKa = 11.84 WLEE178 pKa = 4.28 LEE180 pKa = 4.15 ALQDD184 pKa = 3.64 EE185 pKa = 4.8 SLSVAKK191 pKa = 10.41 LARR194 pKa = 11.84 PEE196 pKa = 4.54 RR197 pKa = 11.84 INTDD201 pKa = 3.16 ALLLKK206 pKa = 9.21 PQQFGAHH213 pKa = 6.31 PFEE216 pKa = 5.15 VDD218 pKa = 2.92 SLMIRR223 pKa = 11.84 CVSNWKK229 pKa = 10.35 ADD231 pKa = 3.77 TPSDD235 pKa = 3.55 GLQDD239 pKa = 4.4 HH240 pKa = 7.13 FDD242 pKa = 3.72 CHH244 pKa = 5.03 FHH246 pKa = 7.42 VMRR249 pKa = 11.84 EE250 pKa = 4.38 LNWKK254 pKa = 8.55 AQQIADD260 pKa = 3.73 EE261 pKa = 4.38 DD262 pKa = 4.06 TLRR265 pKa = 11.84 RR266 pKa = 11.84 HH267 pKa = 5.64 HH268 pKa = 6.98 VINITDD274 pKa = 3.53 RR275 pKa = 11.84 AEE277 pKa = 4.05 WFDD280 pKa = 5.15 HH281 pKa = 6.61 FDD283 pKa = 3.36 EE284 pKa = 4.71 DD285 pKa = 3.92 HH286 pKa = 7.11 PPRR289 pKa = 11.84 ALVKK293 pKa = 10.09 QYY295 pKa = 8.43 PQHH298 pKa = 5.55 VTTHH302 pKa = 6.91 DD303 pKa = 3.73 KK304 pKa = 11.31 DD305 pKa = 3.81 NEE307 pKa = 4.43 LIALFPSICLSFDD320 pKa = 4.05 LDD322 pKa = 3.06 WDD324 pKa = 3.96 YY325 pKa = 12.22 GEE327 pKa = 5.28 NNYY330 pKa = 10.68 GMMDD334 pKa = 3.88 RR335 pKa = 11.84 SRR337 pKa = 11.84 TGAMCGYY344 pKa = 9.12 FLPPSEE350 pKa = 5.09 DD351 pKa = 4.04 CDD353 pKa = 3.77 DD354 pKa = 3.68 ASYY357 pKa = 10.42 WEE359 pKa = 3.8 QRR361 pKa = 11.84 RR362 pKa = 11.84 NIRR365 pKa = 11.84 TNCSGFANVEE375 pKa = 3.87 RR376 pKa = 11.84 GILRR380 pKa = 11.84 VAGEE384 pKa = 3.71 IMDD387 pKa = 3.95 YY388 pKa = 10.87 FRR390 pKa = 11.84 DD391 pKa = 3.89 DD392 pKa = 3.67 PEE394 pKa = 5.04 FYY396 pKa = 11.08 AEE398 pKa = 5.32 LGDD401 pKa = 3.73 TRR403 pKa = 11.84 DD404 pKa = 4.01 FILNKK409 pKa = 9.96 ILVGWSRR416 pKa = 11.84 PDD418 pKa = 3.15 VKK420 pKa = 10.66 HH421 pKa = 6.15 YY422 pKa = 8.51 STSDD426 pKa = 3.25 FRR428 pKa = 11.84 RR429 pKa = 11.84 DD430 pKa = 3.28 FQRR433 pKa = 11.84 QIDD436 pKa = 3.91 GRR438 pKa = 11.84 SPQDD442 pKa = 3.27 QPPIQQTYY450 pKa = 10.19 DD451 pKa = 3.02 KK452 pKa = 11.08 VSRR455 pKa = 11.84 TFVNEE460 pKa = 3.71 IKK462 pKa = 10.57 SCEE465 pKa = 3.98 LQNWNVWKK473 pKa = 10.41 RR474 pKa = 11.84 IKK476 pKa = 9.45 QLRR479 pKa = 11.84 VQVRR483 pKa = 11.84 DD484 pKa = 3.72 QNCAPALYY492 pKa = 10.35 LRR494 pKa = 11.84 WTNTIFLEE502 pKa = 4.31 MQRR505 pKa = 11.84 RR506 pKa = 11.84 MYY508 pKa = 11.16 SFVTEE513 pKa = 5.0 DD514 pKa = 3.01 SCQVNRR520 pKa = 11.84 EE521 pKa = 4.0 NQDD524 pKa = 2.66 ADD526 pKa = 3.38 VGQRR530 pKa = 11.84 VPADD534 pKa = 3.49 MPIQQAGNWTQQVGAQGRR552 pKa = 11.84 FWIDD556 pKa = 3.2 DD557 pKa = 3.7 EE558 pKa = 5.74 GNTTTEE564 pKa = 5.58 DD565 pKa = 3.72 PDD567 pKa = 3.59 QNQITLDD574 pKa = 3.67 LGKK577 pKa = 10.91 LNISDD582 pKa = 3.43 IFGAIAQHH590 pKa = 6.4 ANPLTFALEE599 pKa = 4.41 GASQGLDD606 pKa = 2.92 KK607 pKa = 11.26 AFEE610 pKa = 4.32 LLQFLKK616 pKa = 10.59 PVNTTEE622 pKa = 3.95 HH623 pKa = 4.78 VMKK626 pKa = 10.51 IIRR629 pKa = 11.84 APTWSEE635 pKa = 3.96 KK636 pKa = 9.79 IAGAIRR642 pKa = 11.84 LAEE645 pKa = 3.84 THH647 pKa = 6.55 GILWEE652 pKa = 4.2 TKK654 pKa = 9.48 LLQKK658 pKa = 10.21 VFQHH662 pKa = 7.4 AYY664 pKa = 9.29 RR665 pKa = 11.84 ALNSAVSSCGLEE677 pKa = 3.89 IPEE680 pKa = 4.69 EE681 pKa = 4.22 GLPKK685 pKa = 10.1 QQAGVNDD692 pKa = 4.59 DD693 pKa = 3.97 DD694 pKa = 4.4 KK695 pKa = 11.06 TWTSSMLGFEE705 pKa = 5.18 CPTLSPVAFAAVTIGCSIAAMLGLTIVTRR734 pKa = 11.84 GGDD737 pKa = 3.56 SQGFLSKK744 pKa = 9.77 WGKK747 pKa = 10.44 DD748 pKa = 3.31 FGLLARR754 pKa = 11.84 GITSIGALFMQIYY767 pKa = 9.28 TGVKK771 pKa = 8.07 WFLTSVCGFKK781 pKa = 9.96 IDD783 pKa = 5.35 DD784 pKa = 3.84 PQEE787 pKa = 4.1 RR788 pKa = 11.84 NRR790 pKa = 11.84 EE791 pKa = 3.99 RR792 pKa = 11.84 LISALTEE799 pKa = 3.75 LRR801 pKa = 11.84 LKK803 pKa = 10.54 VSHH806 pKa = 6.73 IVSDD810 pKa = 4.78 LNANMQDD817 pKa = 3.43 FLLNPGVYY825 pKa = 9.45 TEE827 pKa = 4.32 ITRR830 pKa = 11.84 SADD833 pKa = 3.01 KK834 pKa = 10.76 LYY836 pKa = 11.22 AEE838 pKa = 4.96 ILEE841 pKa = 4.06 EE842 pKa = 3.97 HH843 pKa = 5.56 QRR845 pKa = 11.84 KK846 pKa = 8.7 NFNSHH851 pKa = 5.11 MKK853 pKa = 10.15 LATEE857 pKa = 3.94 MWEE860 pKa = 4.0 EE861 pKa = 3.75 AKK863 pKa = 10.61 KK864 pKa = 10.54 LKK866 pKa = 10.64 KK867 pKa = 10.38 LICTAQTQCGFKK879 pKa = 9.0 QTPACIWIYY888 pKa = 11.02 GKK890 pKa = 8.42 TGCGKK895 pKa = 8.89 TNFCKK900 pKa = 10.77 YY901 pKa = 9.31 LAKK904 pKa = 10.58 RR905 pKa = 11.84 LQQRR909 pKa = 11.84 SGRR912 pKa = 11.84 LLLQYY917 pKa = 9.55 QRR919 pKa = 11.84 KK920 pKa = 7.48 ATNKK924 pKa = 9.61 HH925 pKa = 3.95 WDD927 pKa = 3.85 GYY929 pKa = 9.25 QGQPIVIFDD938 pKa = 5.16 DD939 pKa = 3.54 IGSKK943 pKa = 9.9 IGQDD947 pKa = 3.57 DD948 pKa = 4.3 LGDD951 pKa = 3.59 FASIMDD957 pKa = 4.49 AGAWITPQADD967 pKa = 3.85 LQDD970 pKa = 3.71 KK971 pKa = 9.81 GKK973 pKa = 8.87 PFCSTIVVCTSNFPTIDD990 pKa = 3.32 AKK992 pKa = 10.97 EE993 pKa = 4.06 LLRR996 pKa = 11.84 DD997 pKa = 3.59 PEE999 pKa = 3.63 ITMRR1003 pKa = 11.84 RR1004 pKa = 11.84 RR1005 pKa = 11.84 HH1006 pKa = 5.84 IVVEE1010 pKa = 4.32 MVDD1013 pKa = 3.63 KK1014 pKa = 10.42 TWTKK1018 pKa = 8.74 EE1019 pKa = 3.6 TAGDD1023 pKa = 3.52 VRR1025 pKa = 11.84 RR1026 pKa = 11.84 DD1027 pKa = 3.12 IEE1029 pKa = 4.39 DD1030 pKa = 3.52 FQEE1033 pKa = 4.78 VEE1035 pKa = 4.19 FHH1037 pKa = 6.72 LKK1039 pKa = 10.67 NPMAVTKK1046 pKa = 10.2 GRR1048 pKa = 11.84 PRR1050 pKa = 11.84 NFYY1053 pKa = 10.19 DD1054 pKa = 3.46 QSGDD1058 pKa = 3.51 DD1059 pKa = 3.86 SCVVEE1064 pKa = 4.92 AEE1066 pKa = 4.13 WLVEE1070 pKa = 3.98 EE1071 pKa = 4.36 VLKK1074 pKa = 10.97 IEE1076 pKa = 3.99 ARR1078 pKa = 11.84 EE1079 pKa = 3.62 RR1080 pKa = 11.84 AEE1082 pKa = 4.02 FQKK1085 pKa = 8.07 TTQDD1089 pKa = 3.15 QAGFAEE1095 pKa = 4.6 LRR1097 pKa = 11.84 STTPHH1102 pKa = 5.63 ATKK1105 pKa = 9.99 TPKK1108 pKa = 10.16 FYY1110 pKa = 10.18 PRR1112 pKa = 11.84 KK1113 pKa = 8.09 PTPQPKK1119 pKa = 9.58 EE1120 pKa = 3.8 ALEE1123 pKa = 5.17 DD1124 pKa = 3.84 SDD1126 pKa = 6.52 DD1127 pKa = 5.28 DD1128 pKa = 5.57 DD1129 pKa = 5.64 FDD1131 pKa = 5.0 YY1132 pKa = 11.47 DD1133 pKa = 4.3 PALLTWNPSSTKK1145 pKa = 10.14 FSEE1148 pKa = 4.08 QKK1150 pKa = 10.33 QEE1152 pKa = 3.67 IEE1154 pKa = 3.63 RR1155 pKa = 11.84 HH1156 pKa = 4.76 IRR1158 pKa = 11.84 VLSTCIRR1165 pKa = 11.84 QMEE1168 pKa = 4.51 KK1169 pKa = 10.83 ALSDD1173 pKa = 3.73 PDD1175 pKa = 4.53 LEE1177 pKa = 4.95 LEE1179 pKa = 5.01 RR1180 pKa = 11.84 DD1181 pKa = 3.71 DD1182 pKa = 5.37 TPIDD1186 pKa = 3.97 DD1187 pKa = 4.7 LLEE1190 pKa = 4.4 ARR1192 pKa = 11.84 DD1193 pKa = 3.53 RR1194 pKa = 11.84 WRR1196 pKa = 11.84 KK1197 pKa = 8.82 EE1198 pKa = 3.51 LLVDD1202 pKa = 4.5 PKK1204 pKa = 10.9 KK1205 pKa = 10.04 QAKK1208 pKa = 8.19 KK1209 pKa = 10.6 EE1210 pKa = 3.99 KK1211 pKa = 7.93 MKK1213 pKa = 11.22 KK1214 pKa = 7.98 MFKK1217 pKa = 9.83 KK1218 pKa = 10.48 AKK1220 pKa = 8.14 SPKK1223 pKa = 9.74 VRR1225 pKa = 11.84 TQEE1228 pKa = 3.87 NPEE1231 pKa = 4.08 PEE1233 pKa = 4.64 SFYY1236 pKa = 11.16 KK1237 pKa = 10.31 PWWNKK1242 pKa = 9.59 RR1243 pKa = 11.84 PQDD1246 pKa = 4.51 LGDD1249 pKa = 3.85 EE1250 pKa = 4.34 KK1251 pKa = 11.05 EE1252 pKa = 4.14 KK1253 pKa = 11.2 ALVIPTVATIVKK1265 pKa = 9.5 QVAKK1269 pKa = 10.74 LNEE1272 pKa = 4.15 TLKK1275 pKa = 10.32 EE1276 pKa = 4.09 PKK1278 pKa = 9.74 SEE1280 pKa = 4.2 STLPPSWVAPTRR1292 pKa = 11.84 GDD1294 pKa = 3.91 GRR1296 pKa = 11.84 CLLHH1300 pKa = 6.97 AVQPFTGMSVQEE1312 pKa = 4.33 LCDD1315 pKa = 4.26 KK1316 pKa = 10.88 IIAFTANEE1324 pKa = 4.05 DD1325 pKa = 4.01 TLTGLFDD1332 pKa = 3.96 MDD1334 pKa = 4.01 PEE1336 pKa = 4.59 EE1337 pKa = 4.18 IAGIMGSQAWRR1348 pKa = 11.84 QEE1350 pKa = 3.62 IADD1353 pKa = 4.44 WILTGNFHH1361 pKa = 7.23 HH1362 pKa = 6.69 EE1363 pKa = 3.73 AATRR1367 pKa = 11.84 AGTLLGKK1374 pKa = 10.62 LFGIPIAYY1382 pKa = 9.35 IKK1384 pKa = 10.36 WSDD1387 pKa = 2.92 LHH1389 pKa = 6.61 QRR1391 pKa = 11.84 YY1392 pKa = 8.36 VVQGAAGDD1400 pKa = 3.68 HH1401 pKa = 6.61 SIPVFYY1407 pKa = 10.61 ACQHH1411 pKa = 5.95 FSTWLGGEE1419 pKa = 4.25 LPKK1422 pKa = 10.61 QQGGNVEE1429 pKa = 4.05 MTSRR1433 pKa = 11.84 KK1434 pKa = 9.39 IPVFLGPAGTGKK1446 pKa = 7.79 THH1448 pKa = 7.65 LATKK1452 pKa = 9.46 FSPDD1456 pKa = 3.16 KK1457 pKa = 10.44 IAPSEE1462 pKa = 3.91 LRR1464 pKa = 11.84 PEE1466 pKa = 4.99 FINGKK1471 pKa = 5.63 TLHH1474 pKa = 6.65 ISDD1477 pKa = 4.29 FTSTAEE1483 pKa = 3.74 NFEE1486 pKa = 4.19 AFRR1489 pKa = 11.84 AFVMEE1494 pKa = 4.6 YY1495 pKa = 9.63 YY1496 pKa = 10.6 DD1497 pKa = 3.77 KK1498 pKa = 10.75 STTEE1502 pKa = 4.12 GVVATGNGTTFEE1514 pKa = 4.58 KK1515 pKa = 10.66 RR1516 pKa = 11.84 LSDD1519 pKa = 4.89 LVEE1522 pKa = 4.2 EE1523 pKa = 4.79 DD1524 pKa = 3.08 QDD1526 pKa = 3.86 LAEE1529 pKa = 4.36 AFLRR1533 pKa = 11.84 RR1534 pKa = 11.84 LEE1536 pKa = 4.25 IYY1538 pKa = 10.34 DD1539 pKa = 3.89 FKK1541 pKa = 11.3 FKK1543 pKa = 11.3 NKK1545 pKa = 9.23 FLGWSSYY1552 pKa = 9.35 TRR1554 pKa = 11.84 DD1555 pKa = 4.05 DD1556 pKa = 3.38 VDD1558 pKa = 3.99 ALGGDD1563 pKa = 3.92 SEE1565 pKa = 5.55 KK1566 pKa = 9.92 ITTLVSIHH1574 pKa = 6.33 KK1575 pKa = 10.12 RR1576 pKa = 11.84 STLEE1580 pKa = 3.9 AMTQEE1585 pKa = 4.37 EE1586 pKa = 4.84 CLSEE1590 pKa = 4.23 VFEE1593 pKa = 4.44 AKK1595 pKa = 10.11 ARR1597 pKa = 11.84 NTQVYY1602 pKa = 9.99 INTSLLRR1609 pKa = 11.84 LNPASYY1615 pKa = 8.71 THH1617 pKa = 6.89 IVQVDD1622 pKa = 3.43 MTLRR1626 pKa = 11.84 TLLEE1630 pKa = 4.41 RR1631 pKa = 11.84 PLSSWAAEE1639 pKa = 4.08 IPQVIAGTSRR1649 pKa = 11.84 LGIRR1653 pKa = 11.84 LIKK1656 pKa = 10.77 GCFTDD1661 pKa = 3.32 KK1662 pKa = 11.23 GVMHH1666 pKa = 7.62 LFTSLTGKK1674 pKa = 9.81 HH1675 pKa = 5.16 YY1676 pKa = 11.25 NRR1678 pKa = 11.84 MGTVEE1683 pKa = 5.54 KK1684 pKa = 10.86 YY1685 pKa = 10.65 LQTINNSRR1693 pKa = 11.84 IKK1695 pKa = 10.64 SVAPCSLKK1703 pKa = 10.42 IICRR1707 pKa = 11.84 DD1708 pKa = 3.38 TTLVAVTDD1716 pKa = 4.29 DD1717 pKa = 4.7 DD1718 pKa = 4.74 LFIHH1722 pKa = 6.81 IGMLGEE1728 pKa = 4.09 MIYY1731 pKa = 10.61 YY1732 pKa = 10.18 KK1733 pKa = 10.17 RR1734 pKa = 11.84 DD1735 pKa = 3.11 GVGYY1739 pKa = 8.1 RR1740 pKa = 11.84 TDD1742 pKa = 3.31 GDD1744 pKa = 3.94 GFEE1747 pKa = 4.99 EE1748 pKa = 4.51 EE1749 pKa = 4.17 VDD1751 pKa = 3.22 PDD1753 pKa = 4.85 FFDD1756 pKa = 4.99 FLCTLKK1762 pKa = 11.08 GVVCDD1767 pKa = 3.78 NDD1769 pKa = 3.95 QVPEE1773 pKa = 4.03 PAEE1776 pKa = 4.23 VPSGGPDD1783 pKa = 3.23 EE1784 pKa = 5.28 VPPSDD1789 pKa = 3.74 EE1790 pKa = 4.09 AQFMNPWLRR1799 pKa = 11.84 SLFTLGNIFVKK1810 pKa = 10.58 LLSVFGAFWAISDD1823 pKa = 4.15 SVNNTGKK1830 pKa = 10.08 EE1831 pKa = 3.9 KK1832 pKa = 10.49 KK1833 pKa = 8.96 RR1834 pKa = 11.84 QEE1836 pKa = 4.12 RR1837 pKa = 11.84 ATLEE1841 pKa = 4.01 QIQIPDD1847 pKa = 3.71 EE1848 pKa = 4.31 EE1849 pKa = 4.64 QPGPRR1854 pKa = 11.84 EE1855 pKa = 4.07 ARR1857 pKa = 11.84 STKK1860 pKa = 9.58 NNRR1863 pKa = 11.84 RR1864 pKa = 11.84 AMNEE1868 pKa = 4.03 SPTGGVTPEE1877 pKa = 4.06 EE1878 pKa = 3.95 RR1879 pKa = 11.84 DD1880 pKa = 3.69 SFLLEE1885 pKa = 3.86 ANAYY1889 pKa = 8.44 DD1890 pKa = 3.86 IPNNKK1895 pKa = 8.93 KK1896 pKa = 10.33 DD1897 pKa = 3.72 EE1898 pKa = 4.22 EE1899 pKa = 4.52 VVLEE1903 pKa = 4.27 KK1904 pKa = 10.95 KK1905 pKa = 10.24 KK1906 pKa = 10.87 GRR1908 pKa = 11.84 NKK1910 pKa = 9.92 KK1911 pKa = 10.27 AKK1913 pKa = 9.36 TPEE1916 pKa = 3.82 EE1917 pKa = 4.35 SPGKK1921 pKa = 9.76 GQHH1924 pKa = 6.14 DD1925 pKa = 3.12 WRR1927 pKa = 11.84 MPNRR1931 pKa = 11.84 FRR1933 pKa = 11.84 LEE1935 pKa = 3.86 EE1936 pKa = 4.02 FEE1938 pKa = 4.37 EE1939 pKa = 4.59 LSEE1942 pKa = 4.02 EE1943 pKa = 5.16 LEE1945 pKa = 4.53 DD1946 pKa = 5.81 LSDD1949 pKa = 5.55 DD1950 pKa = 3.74 IQAEE1954 pKa = 4.27 ATKK1957 pKa = 10.6 DD1958 pKa = 3.74 PQCDD1962 pKa = 3.38 ALIDD1966 pKa = 4.15 LLPKK1970 pKa = 10.61 KK1971 pKa = 9.11 MVRR1974 pKa = 11.84 VLGGQNNTFAYY1985 pKa = 9.18 GIHH1988 pKa = 5.26 YY1989 pKa = 10.13 RR1990 pKa = 11.84 GRR1992 pKa = 11.84 CVVTVKK1998 pKa = 10.69 HH1999 pKa = 6.01 LGKK2002 pKa = 10.71 GPFWIQPIQGGDD2014 pKa = 2.75 KK2015 pKa = 9.07 WKK2017 pKa = 10.61 ARR2019 pKa = 11.84 SVFQSQKK2026 pKa = 10.63 KK2027 pKa = 10.01 DD2028 pKa = 3.58 FQILEE2033 pKa = 4.28 MEE2035 pKa = 4.54 EE2036 pKa = 4.3 KK2037 pKa = 10.62 APQGKK2042 pKa = 10.08 DD2043 pKa = 2.8 ITGHH2047 pKa = 4.89 FRR2049 pKa = 11.84 ARR2051 pKa = 11.84 GLTGSVEE2058 pKa = 4.23 HH2059 pKa = 5.21 QHH2061 pKa = 5.56 GVLMIPNRR2069 pKa = 11.84 INHH2072 pKa = 6.05 GSVNCWNVTLEE2083 pKa = 3.92 KK2084 pKa = 10.08 EE2085 pKa = 4.28 QLVSCLDD2092 pKa = 3.95 ANDD2095 pKa = 4.15 SVQTTLGAHH2104 pKa = 5.73 YY2105 pKa = 9.71 KK2106 pKa = 9.72 VAAGGIASPNCTRR2119 pKa = 11.84 PGDD2122 pKa = 3.87 CGAPVLLKK2130 pKa = 10.67 DD2131 pKa = 3.62 PKK2133 pKa = 10.76 SPKK2136 pKa = 9.72 KK2137 pKa = 10.76 LLGIHH2142 pKa = 5.6 TAGNQSVSQMCFITQEE2158 pKa = 4.1 DD2159 pKa = 5.13 LIQNVMPKK2167 pKa = 10.27 EE2168 pKa = 4.37 KK2169 pKa = 9.98 IEE2171 pKa = 4.06 SLPYY2175 pKa = 7.99 QQCVFDD2181 pKa = 5.19 PDD2183 pKa = 4.0 CEE2185 pKa = 4.24 APNEE2189 pKa = 4.07 EE2190 pKa = 4.38 KK2191 pKa = 10.72 AILIDD2196 pKa = 3.69 STGEE2200 pKa = 3.74 QIKK2203 pKa = 7.55 MHH2205 pKa = 6.44 RR2206 pKa = 11.84 PKK2208 pKa = 9.83 KK2209 pKa = 7.88 TSFWRR2214 pKa = 11.84 SPLEE2218 pKa = 3.61 WKK2220 pKa = 10.07 EE2221 pKa = 3.43 RR2222 pKa = 11.84 LKK2224 pKa = 11.21 YY2225 pKa = 10.24 YY2226 pKa = 10.3 FEE2228 pKa = 5.08 PSVLDD2233 pKa = 3.82 AKK2235 pKa = 10.51 DD2236 pKa = 3.36 PRR2238 pKa = 11.84 MEE2240 pKa = 4.45 KK2241 pKa = 10.3 PVDD2244 pKa = 3.44 PMKK2247 pKa = 10.78 QGLEE2251 pKa = 3.86 KK2252 pKa = 10.17 WIVPPPRR2259 pKa = 11.84 VHH2261 pKa = 6.46 LASVEE2266 pKa = 4.25 AIAEE2270 pKa = 4.14 EE2271 pKa = 4.26 VADD2274 pKa = 3.66 YY2275 pKa = 10.81 LAYY2278 pKa = 10.31 NVQKK2282 pKa = 10.67 RR2283 pKa = 11.84 GIKK2286 pKa = 9.43 TKK2288 pKa = 10.49 VFSKK2292 pKa = 10.86 KK2293 pKa = 8.81 EE2294 pKa = 3.91 AINRR2298 pKa = 11.84 NTAYY2302 pKa = 10.71 VDD2304 pKa = 3.62 SNPLYY2309 pKa = 9.93 RR2310 pKa = 11.84 RR2311 pKa = 11.84 SSAGFPWSTEE2321 pKa = 3.43 PGVRR2325 pKa = 11.84 QKK2327 pKa = 11.42 DD2328 pKa = 3.58 PYY2330 pKa = 10.69 IVFNEE2335 pKa = 4.1 SEE2337 pKa = 4.72 GIHH2340 pKa = 6.47 EE2341 pKa = 4.78 INMEE2345 pKa = 4.04 HH2346 pKa = 7.26 ALGRR2350 pKa = 11.84 KK2351 pKa = 9.04 LNASIDD2357 pKa = 3.9 QLVAAAVSGKK2367 pKa = 8.62 RR2368 pKa = 11.84 TATVFQGSLKK2378 pKa = 10.71 DD2379 pKa = 3.63 EE2380 pKa = 4.41 PLKK2383 pKa = 10.84 KK2384 pKa = 10.41 EE2385 pKa = 3.97 KK2386 pKa = 10.45 IKK2388 pKa = 10.54 IGKK2391 pKa = 6.82 TRR2393 pKa = 11.84 SFAASPLDD2401 pKa = 3.29 YY2402 pKa = 10.45 TIAVRR2407 pKa = 11.84 QYY2409 pKa = 10.31 FGAATAALKK2418 pKa = 6.86 TTRR2421 pKa = 11.84 DD2422 pKa = 3.43 LHH2424 pKa = 6.01 PIKK2427 pKa = 10.86 VGINPHH2433 pKa = 4.65 SRR2435 pKa = 11.84 EE2436 pKa = 3.56 WDD2438 pKa = 3.56 LLAKK2442 pKa = 9.86 SLKK2445 pKa = 10.43 KK2446 pKa = 10.32 KK2447 pKa = 10.65 GPYY2450 pKa = 10.05 CFDD2453 pKa = 3.88 YY2454 pKa = 10.89 DD2455 pKa = 3.84 VKK2457 pKa = 10.77 RR2458 pKa = 11.84 WDD2460 pKa = 3.28 SSLPRR2465 pKa = 11.84 VFIEE2469 pKa = 4.21 QVPTIWNRR2477 pKa = 11.84 IYY2479 pKa = 11.02 QKK2481 pKa = 9.53 TDD2483 pKa = 3.5 PFWKK2487 pKa = 10.13 PSDD2490 pKa = 3.57 DD2491 pKa = 4.01 VIRR2494 pKa = 11.84 TNLHH2498 pKa = 5.7 KK2499 pKa = 10.55 AIHH2502 pKa = 6.47 GPLIANGSSVLQMPGGMASGYY2523 pKa = 8.77 PCTVEE2528 pKa = 4.27 EE2529 pKa = 4.3 NSITHH2534 pKa = 6.38 LMMVVYY2540 pKa = 9.27 LWKK2543 pKa = 9.38 MLCMLYY2549 pKa = 10.52 NKK2551 pKa = 9.81 PKK2553 pKa = 10.59 DD2554 pKa = 3.09 WGTLRR2559 pKa = 11.84 GFLKK2563 pKa = 10.36 RR2564 pKa = 11.84 VSPVIYY2570 pKa = 10.61 GDD2572 pKa = 4.81 DD2573 pKa = 3.57 YY2574 pKa = 11.69 LNNVSPNVRR2583 pKa = 11.84 HH2584 pKa = 5.48 MWNFSTVSKK2593 pKa = 10.7 AFKK2596 pKa = 10.01 HH2597 pKa = 4.94 LLRR2600 pKa = 11.84 YY2601 pKa = 7.5 EE2602 pKa = 4.07 VCSPDD2607 pKa = 3.52 KK2608 pKa = 11.03 EE2609 pKa = 4.35 SDD2611 pKa = 2.92 EE2612 pKa = 4.35 RR2613 pKa = 11.84 RR2614 pKa = 11.84 ITKK2617 pKa = 10.21 SLEE2620 pKa = 3.59 QCTFLHH2626 pKa = 6.35 RR2627 pKa = 11.84 RR2628 pKa = 11.84 FVQHH2632 pKa = 6.73 EE2633 pKa = 4.04 DD2634 pKa = 3.63 LYY2636 pKa = 11.3 LGPLTDD2642 pKa = 5.28 DD2643 pKa = 4.72 SIEE2646 pKa = 4.22 KK2647 pKa = 9.61 MLSLTQRR2654 pKa = 11.84 RR2655 pKa = 11.84 VRR2657 pKa = 11.84 HH2658 pKa = 4.89 VWTDD2662 pKa = 3.07 EE2663 pKa = 4.07 DD2664 pKa = 3.69 QTISYY2669 pKa = 11.14 DD2670 pKa = 3.4 MEE2672 pKa = 4.71 TIQATMYY2679 pKa = 10.61 SFMRR2683 pKa = 11.84 EE2684 pKa = 3.56 AVLQGKK2690 pKa = 8.4 PFFQSAQHH2698 pKa = 5.66 HH2699 pKa = 5.88 LEE2701 pKa = 4.15 RR2702 pKa = 11.84 VSRR2705 pKa = 11.84 EE2706 pKa = 3.79 YY2707 pKa = 10.54 EE2708 pKa = 3.84 IPIPPMEE2715 pKa = 4.42 SFEE2718 pKa = 4.11 QQFLEE2723 pKa = 5.17 LIDD2726 pKa = 4.15 RR2727 pKa = 11.84 SPIRR2731 pKa = 3.89
Molecular weight: 311.94 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 5.875
IPC2_protein 5.817
IPC_protein 5.881
Toseland 6.224
ProMoST 6.237
Dawson 6.148
Bjellqvist 6.16
Wikipedia 6.148
Rodwell 6.135
Grimsley 6.364
Solomon 6.148
Lehninger 6.135
Nozaki 6.415
DTASelect 6.605
Thurlkill 6.605
EMBOSS 6.576
Sillero 6.517
Patrickios 4.355
IPC_peptide 6.16
IPC2_peptide 6.465
IPC2.peptide.svr19 6.469
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2
0
2
3249
518
2731
1624.5
184.5
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.525 ± 0.797
1.6 ± 0.708
6.894 ± 0.069
6.802 ± 0.703
4.555 ± 0.331
5.971 ± 0.687
2.647 ± 0.125
5.448 ± 0.216
6.248 ± 1.493
8.126 ± 0.3
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.247 ± 0.062
3.817 ± 0.217
5.663 ± 0.259
4.986 ± 0.275
5.725 ± 0.647
5.848 ± 0.652
6.433 ± 0.26
5.94 ± 1.383
1.939 ± 0.004
2.585 ± 0.232
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here