Mycobacterium phage Fishburne

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Pclasvirinae; Fishburnevirus; Mycobacterium virus Fishburne

Average proteome isoelectric point is 6.17

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 77 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|R4T9X1|R4T9X1_9CAUD Uncharacterized protein OS=Mycobacterium phage Fishburne OX=1327765 GN=41 PE=4 SV=1
MM1 pKa = 7.78SDD3 pKa = 3.47YY4 pKa = 11.31LDD6 pKa = 4.12ASGLTDD12 pKa = 4.02AGLPDD17 pKa = 4.67EE18 pKa = 5.45YY19 pKa = 11.43LDD21 pKa = 6.24LDD23 pKa = 4.75DD24 pKa = 6.48LDD26 pKa = 5.03LPRR29 pKa = 11.84STAPQCEE36 pKa = 4.53HH37 pKa = 7.06PPVACTAEE45 pKa = 4.19CRR47 pKa = 11.84GVRR50 pKa = 11.84AGSTVARR57 pKa = 11.84FLVTLGTTLVAFALVLALTLGAVLAA82 pKa = 4.94

Molecular weight: 8.51 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|R4TQU3|R4TQU3_9CAUD Uncharacterized protein OS=Mycobacterium phage Fishburne OX=1327765 GN=35 PE=4 SV=1
MM1 pKa = 7.69TPRR4 pKa = 11.84QTGSYY9 pKa = 9.41RR10 pKa = 11.84LVSSGRR16 pKa = 11.84EE17 pKa = 3.97GQFPMVNRR25 pKa = 11.84WRR27 pKa = 11.84TWQFAQSLSARR38 pKa = 11.84TVDD41 pKa = 3.31EE42 pKa = 3.98RR43 pKa = 11.84VATVRR48 pKa = 11.84RR49 pKa = 11.84MAVWCGVEE57 pKa = 3.95PEE59 pKa = 4.35FAQVEE64 pKa = 4.91HH65 pKa = 6.35IVSWLAEE72 pKa = 4.26GGQWSARR79 pKa = 11.84TRR81 pKa = 11.84WTYY84 pKa = 11.34YY85 pKa = 10.31GALSAWFLWLQQQGHH100 pKa = 5.14RR101 pKa = 11.84HH102 pKa = 6.2DD103 pKa = 4.56NPMVMIGRR111 pKa = 11.84PKK113 pKa = 10.09RR114 pKa = 11.84PKK116 pKa = 9.75SVPRR120 pKa = 11.84PVSNLDD126 pKa = 3.3MQRR129 pKa = 11.84LLAVRR134 pKa = 11.84AHH136 pKa = 6.47KK137 pKa = 9.25RR138 pKa = 11.84TKK140 pKa = 11.33AMILLAAFQGLRR152 pKa = 11.84VHH154 pKa = 7.38EE155 pKa = 4.11IAQIKK160 pKa = 10.05GEE162 pKa = 4.07HH163 pKa = 6.8LDD165 pKa = 4.12LIEE168 pKa = 4.13RR169 pKa = 11.84TMTVTGKK176 pKa = 11.13GNVTATLPLHH186 pKa = 5.96HH187 pKa = 6.79RR188 pKa = 11.84VVEE191 pKa = 4.15IAYY194 pKa = 8.39QMPRR198 pKa = 11.84KK199 pKa = 9.34GHH201 pKa = 6.4WFPGPDD207 pKa = 2.81RR208 pKa = 11.84GHH210 pKa = 5.51QRR212 pKa = 11.84RR213 pKa = 11.84EE214 pKa = 4.31SVSGTIKK221 pKa = 10.28EE222 pKa = 4.01AMIRR226 pKa = 11.84AGVVGSAHH234 pKa = 7.08CLRR237 pKa = 11.84HH238 pKa = 4.94WFGTALLEE246 pKa = 4.69AGVDD250 pKa = 3.56LRR252 pKa = 11.84TVQEE256 pKa = 4.11LMRR259 pKa = 11.84HH260 pKa = 4.7QSLTSTEE267 pKa = 3.62IYY269 pKa = 10.3TRR271 pKa = 11.84VTDD274 pKa = 3.31QRR276 pKa = 11.84RR277 pKa = 11.84AEE279 pKa = 4.58GIEE282 pKa = 4.09RR283 pKa = 11.84IDD285 pKa = 3.88PFRR288 pKa = 11.84VAPSTRR294 pKa = 11.84VSDD297 pKa = 3.72RR298 pKa = 11.84LLAQIAEE305 pKa = 4.42PVEE308 pKa = 4.59GAAGAAVSAAA318 pKa = 3.56

Molecular weight: 35.96 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

77

0

77

15258

29

1256

198.2

21.48

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.335 ± 0.557

0.937 ± 0.118

6.534 ± 0.182

6.154 ± 0.344

2.871 ± 0.189

8.946 ± 0.537

1.966 ± 0.161

4.273 ± 0.147

3.034 ± 0.166

7.956 ± 0.281

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.947 ± 0.106

2.969 ± 0.194

6.226 ± 0.183

3.67 ± 0.286

7.413 ± 0.482

5.322 ± 0.26

6.259 ± 0.244

7.085 ± 0.324

1.861 ± 0.147

2.241 ± 0.121
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski