Pseudooceanicola nitratireducens

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Rhodobacterales; Rhodobacteraceae; Pseudooceanicola

Average proteome isoelectric point is 6.08

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3828 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1I1N3D5|A0A1I1N3D5_9RHOB Hydrogenase maturation protease OS=Pseudooceanicola nitratireducens OX=517719 GN=SAMN05421762_2745 PE=3 SV=1
MM1 pKa = 8.02ADD3 pKa = 3.23GKK5 pKa = 11.19YY6 pKa = 10.22DD7 pKa = 3.6GSSGSDD13 pKa = 3.09NINRR17 pKa = 11.84TSVDD21 pKa = 3.09GGGDD25 pKa = 3.42QVDD28 pKa = 4.62GPDD31 pKa = 3.74GLNDD35 pKa = 3.89SIYY38 pKa = 10.76GYY40 pKa = 10.56GGNDD44 pKa = 3.33TIEE47 pKa = 4.37AGRR50 pKa = 11.84GSDD53 pKa = 3.01TVYY56 pKa = 10.78GGEE59 pKa = 4.19GADD62 pKa = 3.76SIEE65 pKa = 4.16GRR67 pKa = 11.84SGDD70 pKa = 3.61DD71 pKa = 3.16VLYY74 pKa = 11.02GGAGRR79 pKa = 11.84DD80 pKa = 3.9TIEE83 pKa = 4.66GDD85 pKa = 3.19AGNDD89 pKa = 3.6TIYY92 pKa = 10.96VGSGDD97 pKa = 3.82DD98 pKa = 4.52DD99 pKa = 4.26IYY101 pKa = 11.64GEE103 pKa = 4.68SGDD106 pKa = 3.95DD107 pKa = 4.13LIRR110 pKa = 11.84VTRR113 pKa = 11.84GTSAWIDD120 pKa = 3.73GQSGDD125 pKa = 4.07DD126 pKa = 3.54TLDD129 pKa = 3.45FSGLTGTTISSINYY143 pKa = 9.57YY144 pKa = 10.76GGDD147 pKa = 3.63PSNGYY152 pKa = 10.06IIFANGEE159 pKa = 3.83RR160 pKa = 11.84MEE162 pKa = 4.03YY163 pKa = 10.28RR164 pKa = 11.84GIEE167 pKa = 4.67AITGTQDD174 pKa = 2.69GRR176 pKa = 11.84VDD178 pKa = 3.6GSTGADD184 pKa = 3.63SIGDD188 pKa = 4.21GYY190 pKa = 9.7TDD192 pKa = 3.56GQLDD196 pKa = 3.83AVGDD200 pKa = 4.16ADD202 pKa = 4.67GSHH205 pKa = 6.74NDD207 pKa = 3.18SIYY210 pKa = 10.73AYY212 pKa = 10.12GGNDD216 pKa = 3.2TVASGAGNDD225 pKa = 3.77TVYY228 pKa = 11.22GGAGTDD234 pKa = 3.35ALYY237 pKa = 11.06GGAGNDD243 pKa = 3.78ALDD246 pKa = 4.39GGSGDD251 pKa = 4.34DD252 pKa = 4.0ALSGGSGDD260 pKa = 3.7DD261 pKa = 3.31VLYY264 pKa = 11.0GGEE267 pKa = 4.3GWDD270 pKa = 3.49TLYY273 pKa = 11.23SGLGNDD279 pKa = 3.72TYY281 pKa = 11.71YY282 pKa = 11.29GGTGLDD288 pKa = 3.78YY289 pKa = 10.86IDD291 pKa = 5.7FGAEE295 pKa = 3.61TGGVYY300 pKa = 10.47VDD302 pKa = 5.0LSNGTIGGAATGDD315 pKa = 3.73VMGSGIDD322 pKa = 3.6GVFGSNHH329 pKa = 7.59DD330 pKa = 3.69DD331 pKa = 3.47TLIGFDD337 pKa = 3.54PWSPTGDD344 pKa = 3.01VYY346 pKa = 11.68TNVFFGRR353 pKa = 11.84AGNDD357 pKa = 3.1VFDD360 pKa = 4.53GRR362 pKa = 11.84GSPDD366 pKa = 3.39YY367 pKa = 10.23MDD369 pKa = 5.04GGSGDD374 pKa = 4.12DD375 pKa = 3.58TFLMTGTPGSDD386 pKa = 3.3TIIGGEE392 pKa = 4.2GGSDD396 pKa = 2.87QDD398 pKa = 4.23VIDD401 pKa = 4.57FSSVSGPITVDD412 pKa = 3.16YY413 pKa = 10.19TGAEE417 pKa = 4.22AGTITHH423 pKa = 6.83GADD426 pKa = 2.6TVTFSQIEE434 pKa = 4.27RR435 pKa = 11.84IYY437 pKa = 9.69ATSSGDD443 pKa = 3.62SLTGGAGTDD452 pKa = 3.1RR453 pKa = 11.84VEE455 pKa = 4.13GRR457 pKa = 11.84GGSDD461 pKa = 2.91TLYY464 pKa = 10.95GGAGGDD470 pKa = 3.89EE471 pKa = 4.39LSGDD475 pKa = 3.87SGDD478 pKa = 3.82DD479 pKa = 3.31SLYY482 pKa = 11.13GGTGADD488 pKa = 3.24TLYY491 pKa = 11.15GGLGADD497 pKa = 4.22RR498 pKa = 11.84IEE500 pKa = 4.84AGSGDD505 pKa = 3.61DD506 pKa = 4.35QIYY509 pKa = 10.68GGAGADD515 pKa = 4.1AIYY518 pKa = 10.36AGQGADD524 pKa = 3.51SIYY527 pKa = 10.91AASGDD532 pKa = 3.63DD533 pKa = 4.01AIYY536 pKa = 10.36GGSGSDD542 pKa = 3.39VVTLTGPGNYY552 pKa = 10.25ALFGGEE558 pKa = 4.18DD559 pKa = 3.44AGDD562 pKa = 3.86ADD564 pKa = 5.04VDD566 pKa = 3.98VLDD569 pKa = 4.71ISSLGAGGQVTAITYY584 pKa = 10.43NSGDD588 pKa = 4.25DD589 pKa = 3.57EE590 pKa = 5.42SGTITFSNGDD600 pKa = 3.27VATFSGFEE608 pKa = 4.14MIVCFAQGTRR618 pKa = 11.84IDD620 pKa = 3.9TPRR623 pKa = 11.84GPVAVEE629 pKa = 3.67DD630 pKa = 4.54LRR632 pKa = 11.84PGDD635 pKa = 3.9AVMTADD641 pKa = 5.34HH642 pKa = 6.92GPQPLRR648 pKa = 11.84WTGSRR653 pKa = 11.84RR654 pKa = 11.84VPARR658 pKa = 11.84GGSAPVTFATGAIGNTRR675 pKa = 11.84PLTLSPQHH683 pKa = 6.31RR684 pKa = 11.84VLITGWKK691 pKa = 8.98PEE693 pKa = 3.97MLFGEE698 pKa = 4.6AEE700 pKa = 4.14VLVAAKK706 pKa = 10.27HH707 pKa = 5.73LVDD710 pKa = 3.53GHH712 pKa = 6.27EE713 pKa = 4.5VTQSEE718 pKa = 5.03GSQITYY724 pKa = 9.89HH725 pKa = 6.28HH726 pKa = 7.11LCLDD730 pKa = 3.22RR731 pKa = 11.84HH732 pKa = 5.52EE733 pKa = 4.51VLFAEE738 pKa = 5.35GAPVEE743 pKa = 4.42SFLPAEE749 pKa = 4.22EE750 pKa = 4.74ALFSLTPAQRR760 pKa = 11.84RR761 pKa = 11.84DD762 pKa = 3.2LCAALPDD769 pKa = 3.9LDD771 pKa = 4.01AAARR775 pKa = 11.84PARR778 pKa = 11.84PILRR782 pKa = 11.84GYY784 pKa = 8.28EE785 pKa = 3.94AQLLGPSLPPPDD797 pKa = 4.26LRR799 pKa = 11.84RR800 pKa = 11.84AGHH803 pKa = 6.38RR804 pKa = 11.84MANRR808 pKa = 11.84ILL810 pKa = 3.66

Molecular weight: 82.12 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1I1JP25|A0A1I1JP25_9RHOB Chaperone required for the assembly of the F1-ATPase OS=Pseudooceanicola nitratireducens OX=517719 GN=SAMN05421762_1043 PE=3 SV=1
MM1 pKa = 7.35KK2 pKa = 9.44RR3 pKa = 11.84TYY5 pKa = 10.31QPSNLVRR12 pKa = 11.84KK13 pKa = 9.18RR14 pKa = 11.84RR15 pKa = 11.84HH16 pKa = 4.42GFRR19 pKa = 11.84ARR21 pKa = 11.84MATKK25 pKa = 10.37AGRR28 pKa = 11.84KK29 pKa = 8.54ILNARR34 pKa = 11.84RR35 pKa = 11.84ARR37 pKa = 11.84GRR39 pKa = 11.84KK40 pKa = 9.07SLSAA44 pKa = 3.93

Molecular weight: 5.15 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3828

0

3828

1200732

30

2517

313.7

33.99

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.317 ± 0.055

0.852 ± 0.011

6.35 ± 0.039

5.701 ± 0.043

3.622 ± 0.023

8.824 ± 0.041

2.02 ± 0.02

5.137 ± 0.027

3.166 ± 0.032

10.063 ± 0.047

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.814 ± 0.02

2.502 ± 0.023

5.184 ± 0.032

3.305 ± 0.025

6.712 ± 0.038

5.075 ± 0.028

5.577 ± 0.03

7.238 ± 0.03

1.333 ± 0.017

2.21 ± 0.018
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski