Streptomyces sp. WAC06614

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Streptomycetales; Streptomycetaceae; Streptomyces; unclassified Streptomyces

Average proteome isoelectric point is 6.51

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 6875 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A3S0CM17|A0A3S0CM17_9ACTN zf-CGNR domain-containing protein OS=Streptomyces sp. WAC06614 OX=2487416 GN=EF918_23665 PE=4 SV=1
MM1 pKa = 7.2STPEE5 pKa = 3.88QNGYY9 pKa = 7.83EE10 pKa = 4.18APASPEE16 pKa = 3.66PTADD20 pKa = 3.43KK21 pKa = 10.94DD22 pKa = 3.75PYY24 pKa = 9.93ATQTWQTPTWDD35 pKa = 3.22TTYY38 pKa = 10.86QPPQPGLDD46 pKa = 3.19QGQAGLGQGQGQGHH60 pKa = 7.05AGLGQGHH67 pKa = 6.49DD68 pKa = 3.5QGHH71 pKa = 6.88AGLGQGQGQAGLGQGQAGLGQGPAGPEE98 pKa = 3.6AGQAWNAAEE107 pKa = 4.44PAPAAAPAPGQDD119 pKa = 3.14WAPAPDD125 pKa = 3.97QGWAATPAPGTDD137 pKa = 2.77WSAAGQAWAAAAPGAEE153 pKa = 4.69PGWAATPGTGEE164 pKa = 4.02GWTGSRR170 pKa = 11.84PGTEE174 pKa = 4.7DD175 pKa = 3.26PGSAQGWAAPAMTDD189 pKa = 3.42TPGSGSGSGEE199 pKa = 3.28AWAPGAAAPGPTGADD214 pKa = 3.35HH215 pKa = 6.68GWAAGAAQAGPGQSWAAEE233 pKa = 3.79PAAAGGSAEE242 pKa = 5.32AGQSWTAGAAQAEE255 pKa = 4.53PRR257 pKa = 11.84QGWAAGPTGADD268 pKa = 3.4QGWTGGGLGGVPAQQPPAAYY288 pKa = 9.94GSVPAQGASPEE299 pKa = 4.08PLAPAGWFRR308 pKa = 11.84DD309 pKa = 3.77EE310 pKa = 5.05APAAAQPAAYY320 pKa = 9.2PEE322 pKa = 4.17QTAPAPGPGTGSLAAPDD339 pKa = 3.52EE340 pKa = 4.47WFLSFF345 pKa = 5.57

Molecular weight: 33.23 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A3R9V5Z8|A0A3R9V5Z8_9ACTN Uncharacterized protein OS=Streptomyces sp. WAC06614 OX=2487416 GN=EF918_31120 PE=4 SV=1
MM1 pKa = 7.69SKK3 pKa = 9.0RR4 pKa = 11.84TFQPNNRR11 pKa = 11.84RR12 pKa = 11.84RR13 pKa = 11.84AKK15 pKa = 8.7THH17 pKa = 5.15GFRR20 pKa = 11.84LRR22 pKa = 11.84MRR24 pKa = 11.84TRR26 pKa = 11.84AGRR29 pKa = 11.84AILAARR35 pKa = 11.84RR36 pKa = 11.84GKK38 pKa = 10.37GRR40 pKa = 11.84ARR42 pKa = 11.84LSAA45 pKa = 3.91

Molecular weight: 5.24 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

6875

0

6875

2221611

24

4043

323.1

34.46

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

14.242 ± 0.05

0.815 ± 0.009

5.77 ± 0.022

5.572 ± 0.036

2.691 ± 0.016

9.727 ± 0.03

2.344 ± 0.019

2.844 ± 0.02

2.021 ± 0.028

10.498 ± 0.037

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.661 ± 0.012

1.623 ± 0.014

6.421 ± 0.028

2.746 ± 0.017

8.165 ± 0.034

4.681 ± 0.021

6.127 ± 0.026

8.517 ± 0.027

1.498 ± 0.014

2.036 ± 0.013
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski