Apis mellifera associated microvirus 5

Taxonomy: Viruses; Monodnaviria; Sangervirae; Phixviricota; Malgrandaviricetes; Petitvirales; Microviridae; unclassified Microviridae

Average proteome isoelectric point is 6.82

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 8 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A3Q8U5D1|A0A3Q8U5D1_9VIRU Uncharacterized protein OS=Apis mellifera associated microvirus 5 OX=2494780 PE=4 SV=1
MM1 pKa = 7.73EE2 pKa = 4.68FQSAYY7 pKa = 10.32SKK9 pKa = 10.51PFRR12 pKa = 11.84GSDD15 pKa = 3.86LKK17 pKa = 11.3CDD19 pKa = 4.18DD20 pKa = 4.63PSLAQQHH27 pKa = 6.04FKK29 pKa = 11.3DD30 pKa = 4.14DD31 pKa = 3.1VDD33 pKa = 3.97INVLLEE39 pKa = 3.97RR40 pKa = 11.84FKK42 pKa = 11.01VTGQLPQGVRR52 pKa = 11.84LPTYY56 pKa = 11.01GDD58 pKa = 3.48FTGVTDD64 pKa = 4.4FRR66 pKa = 11.84QANEE70 pKa = 4.54AIRR73 pKa = 11.84HH74 pKa = 5.13ANEE77 pKa = 3.94SFMDD81 pKa = 4.07LPANIRR87 pKa = 11.84ARR89 pKa = 11.84FQNDD93 pKa = 2.71PVEE96 pKa = 4.29FVEE99 pKa = 5.2FCSDD103 pKa = 3.88KK104 pKa = 11.36EE105 pKa = 4.02NLPEE109 pKa = 3.92LRR111 pKa = 11.84KK112 pKa = 9.52MGLAPEE118 pKa = 4.23ILGSGAEE125 pKa = 4.13PQVGPSGAAAPEE137 pKa = 4.55GPKK140 pKa = 10.18VV141 pKa = 3.3

Molecular weight: 15.57 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A3Q8U5D3|A0A3Q8U5D3_9VIRU Uncharacterized protein OS=Apis mellifera associated microvirus 5 OX=2494780 PE=4 SV=1
MM1 pKa = 7.87RR2 pKa = 11.84LAQFPLDD9 pKa = 3.54VTVLVDD15 pKa = 4.22TACPWAAVSLQLPYY29 pKa = 9.76LTSLEE34 pKa = 4.62SIMSDD39 pKa = 3.37RR40 pKa = 11.84FPVSRR45 pKa = 11.84YY46 pKa = 8.77ASARR50 pKa = 11.84SFEE53 pKa = 4.17RR54 pKa = 11.84SAGVTRR60 pKa = 11.84VMNVITPLRR69 pKa = 11.84GGWRR73 pKa = 11.84LL74 pKa = 3.22

Molecular weight: 8.27 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

8

0

8

1541

67

541

192.6

21.2

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.955 ± 1.494

1.298 ± 0.712

5.191 ± 0.678

4.478 ± 0.453

5.191 ± 0.928

7.917 ± 0.794

1.947 ± 0.437

4.672 ± 0.46

3.245 ± 0.775

8.631 ± 0.917

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.855 ± 0.329

3.374 ± 0.631

5.581 ± 0.736

3.958 ± 1.019

6.684 ± 0.932

8.501 ± 1.001

5.711 ± 1.139

7.333 ± 0.387

0.844 ± 0.259

3.634 ± 0.525
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski