Gordonia phage Yakult
Average proteome isoelectric point is 6.63
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 74 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A4Y5NY52|A0A4Y5NY52_9CAUD Head-to-tail adaptor OS=Gordonia phage Yakult OX=2584494 GN=10 PE=4 SV=1
MM1 pKa = 7.15 SQQTLAEE8 pKa = 4.07 LQRR11 pKa = 11.84 AIEE14 pKa = 4.02 AHH16 pKa = 5.98 VASQFAEE23 pKa = 4.37 DD24 pKa = 5.0 EE25 pKa = 4.43 PDD27 pKa = 3.18 ATPPIVNCWFLGVGLSGVSDD47 pKa = 4.34 DD48 pKa = 5.51 NIVHH52 pKa = 6.86 DD53 pKa = 3.94 VTYY56 pKa = 10.82 VVSDD60 pKa = 3.77 SPAYY64 pKa = 10.56 ASMGACDD71 pKa = 3.66 IALEE75 pKa = 4.06 QARR78 pKa = 11.84 AHH80 pKa = 5.57 VLYY83 pKa = 11.11 GNDD86 pKa = 3.7 DD87 pKa = 3.66 SAA89 pKa = 5.75
Molecular weight: 9.47 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.788
IPC2_protein 3.859
IPC_protein 3.795
Toseland 3.592
ProMoST 3.986
Dawson 3.795
Bjellqvist 3.961
Wikipedia 3.757
Rodwell 3.63
Grimsley 3.503
Solomon 3.783
Lehninger 3.732
Nozaki 3.935
DTASelect 4.151
Thurlkill 3.668
EMBOSS 3.77
Sillero 3.923
Patrickios 0.477
IPC_peptide 3.77
IPC2_peptide 3.897
IPC2.peptide.svr19 3.822
Protein with the highest isoelectric point:
>tr|A0A4Y5NYD6|A0A4Y5NYD6_9CAUD Uncharacterized protein OS=Gordonia phage Yakult OX=2584494 GN=67 PE=4 SV=1
MM1 pKa = 8.16 AIRR4 pKa = 11.84 CAKK7 pKa = 10.2 SPPHH11 pKa = 6.54 NMTVMRR17 pKa = 11.84 IPAQLIHH24 pKa = 6.11 GTLMGYY30 pKa = 9.95 RR31 pKa = 11.84 YY32 pKa = 9.16 HH33 pKa = 7.09 HH34 pKa = 6.41 CHH36 pKa = 7.2 CEE38 pKa = 3.61 ACRR41 pKa = 11.84 QANTDD46 pKa = 3.0 YY47 pKa = 10.94 CRR49 pKa = 11.84 RR50 pKa = 11.84 QRR52 pKa = 11.84 EE53 pKa = 3.77 KK54 pKa = 10.64 RR55 pKa = 11.84 LARR58 pKa = 11.84 GIPKK62 pKa = 9.73 HH63 pKa = 4.94 VHH65 pKa = 4.59 GTANGYY71 pKa = 10.22 GNYY74 pKa = 10.02 GCRR77 pKa = 11.84 CSRR80 pKa = 11.84 CYY82 pKa = 10.44 AAWSAKK88 pKa = 8.59 MKK90 pKa = 10.3 QRR92 pKa = 11.84 TRR94 pKa = 11.84 RR95 pKa = 11.84 DD96 pKa = 3.09 RR97 pKa = 3.95
Molecular weight: 11.32 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.341
IPC2_protein 9.692
IPC_protein 10.438
Toseland 10.248
ProMoST 10.233
Dawson 10.467
Bjellqvist 10.262
Wikipedia 10.701
Rodwell 10.628
Grimsley 10.555
Solomon 10.54
Lehninger 10.496
Nozaki 10.379
DTASelect 10.233
Thurlkill 10.335
EMBOSS 10.672
Sillero 10.423
Patrickios 10.218
IPC_peptide 10.526
IPC2_peptide 9.721
IPC2.peptide.svr19 8.388
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
74
0
74
15231
31
1620
205.8
22.73
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
10.439 ± 0.446
0.998 ± 0.163
6.9 ± 0.274
5.515 ± 0.328
3.053 ± 0.198
8.437 ± 0.581
2.108 ± 0.223
4.524 ± 0.183
4.53 ± 0.329
7.813 ± 0.226
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.692 ± 0.163
2.935 ± 0.161
5.344 ± 0.195
3.769 ± 0.226
7.124 ± 0.484
5.502 ± 0.221
6.145 ± 0.279
7.071 ± 0.238
2.272 ± 0.165
2.83 ± 0.205
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here