Bacillus virus Blastoid

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Andromedavirus

Average proteome isoelectric point is 6.29

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 78 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|U5PW34|U5PW34_9CAUD Uncharacterized protein OS=Bacillus virus Blastoid OX=1406782 GN=Blastoid_8 PE=4 SV=1
MM1 pKa = 7.55LVRR4 pKa = 11.84MFAEE8 pKa = 3.95YY9 pKa = 10.7DD10 pKa = 3.74VIEE13 pKa = 5.18SYY15 pKa = 10.96PSDD18 pKa = 4.06DD19 pKa = 3.46STMLKK24 pKa = 9.37SDD26 pKa = 5.04RR27 pKa = 11.84YY28 pKa = 9.62IKK30 pKa = 10.56CCNSVDD36 pKa = 4.08LLNNPVPIVVHH47 pKa = 6.42SDD49 pKa = 3.03EE50 pKa = 5.82LSFFHH55 pKa = 6.98YY56 pKa = 10.59DD57 pKa = 3.24KK58 pKa = 10.81EE59 pKa = 4.58TDD61 pKa = 3.21TMIYY65 pKa = 10.16EE66 pKa = 4.16VDD68 pKa = 3.96FHH70 pKa = 8.41DD71 pKa = 5.1IEE73 pKa = 4.5AVYY76 pKa = 10.79

Molecular weight: 8.96 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|U5PS86|U5PS86_9CAUD Aldolase/citrate lyase family protein OS=Bacillus virus Blastoid OX=1406782 GN=Blastoid_26 PE=4 SV=1
MM1 pKa = 7.48SNVNMMISVDD11 pKa = 3.95LNNVPEE17 pKa = 4.22NLATVARR24 pKa = 11.84QVSDD28 pKa = 3.42FKK30 pKa = 11.28IPLRR34 pKa = 11.84QSAVYY39 pKa = 10.54LEE41 pKa = 4.59GSISRR46 pKa = 11.84RR47 pKa = 11.84FSSGGGSKK55 pKa = 11.03GNWKK59 pKa = 9.85RR60 pKa = 11.84LSPATIRR67 pKa = 11.84RR68 pKa = 11.84HH69 pKa = 4.25PHH71 pKa = 5.56RR72 pKa = 11.84SGGIPLSDD80 pKa = 3.34TGRR83 pKa = 11.84LKK85 pKa = 10.92QSVTSGAVKK94 pKa = 10.14QYY96 pKa = 10.82SPKK99 pKa = 9.68QLRR102 pKa = 11.84YY103 pKa = 7.89TLGSNVKK110 pKa = 9.28YY111 pKa = 10.67AQVHH115 pKa = 4.81NFGGGRR121 pKa = 11.84IPARR125 pKa = 11.84PFMYY129 pKa = 10.54VDD131 pKa = 3.35AKK133 pKa = 11.28DD134 pKa = 3.53NQEE137 pKa = 3.93VIKK140 pKa = 10.71IFTEE144 pKa = 4.33FLRR147 pKa = 11.84GVANQQ152 pKa = 3.21

Molecular weight: 16.82 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

78

0

78

15139

40

1029

194.1

22.0

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.143 ± 0.336

0.905 ± 0.122

6.203 ± 0.284

7.874 ± 0.503

4.525 ± 0.19

6.896 ± 0.397

2.147 ± 0.178

6.506 ± 0.177

8.151 ± 0.455

8.131 ± 0.35

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.768 ± 0.181

5.093 ± 0.325

3.256 ± 0.161

3.567 ± 0.165

4.366 ± 0.226

6.295 ± 0.338

5.793 ± 0.3

6.321 ± 0.2

1.136 ± 0.12

3.924 ± 0.24
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski