Lactobacillus selangorensis
Average proteome isoelectric point is 6.54
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2031 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A0R2FH13|A0A0R2FH13_9LACO Abhydrolase_3 domain-containing protein OS=Lactobacillus selangorensis OX=81857 GN=IV38_GL001989 PE=4 SV=1
MM1 pKa = 7.75 AYY3 pKa = 10.08 DD4 pKa = 4.64 LKK6 pKa = 10.34 TLEE9 pKa = 4.17 TDD11 pKa = 4.38 FPNGYY16 pKa = 10.12 DD17 pKa = 3.41 DD18 pKa = 5.07 EE19 pKa = 4.53 YY20 pKa = 11.02 RR21 pKa = 11.84 QKK23 pKa = 11.14 LVDD26 pKa = 4.08 NFTNLNLYY34 pKa = 8.85 WLTALNNIVADD45 pKa = 3.3 NHH47 pKa = 5.25 YY48 pKa = 10.62 QYY50 pKa = 11.89 GFITDD55 pKa = 3.38 NQKK58 pKa = 10.87 AIKK61 pKa = 9.73 QLQDD65 pKa = 2.99 NLAALVDD72 pKa = 3.56 ILGTYY77 pKa = 8.62 GIPLGYY83 pKa = 10.32 DD84 pKa = 3.04 ADD86 pKa = 4.2 GNIVDD91 pKa = 4.9 TSQDD95 pKa = 3.58 DD96 pKa = 3.89 YY97 pKa = 12.08 DD98 pKa = 6.18 DD99 pKa = 5.58 DD100 pKa = 5.79 GSLL103 pKa = 3.89
Molecular weight: 11.7 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.788
IPC2_protein 3.592
IPC_protein 3.592
Toseland 3.363
ProMoST 3.808
Dawson 3.63
Bjellqvist 3.783
Wikipedia 3.63
Rodwell 3.427
Grimsley 3.274
Solomon 3.605
Lehninger 3.567
Nozaki 3.757
DTASelect 4.075
Thurlkill 3.452
EMBOSS 3.63
Sillero 3.732
Patrickios 0.362
IPC_peptide 3.592
IPC2_peptide 3.694
IPC2.peptide.svr19 3.683
Protein with the highest isoelectric point:
>tr|A0A0R2FHP8|A0A0R2FHP8_9LACO Cystathionine beta-lyase PatB OS=Lactobacillus selangorensis OX=81857 GN=IV38_GL001582 PE=4 SV=1
MM1 pKa = 7.91 PYY3 pKa = 9.88 WLQLVVQISFSYY15 pKa = 10.76 LATCAFAICINIPRR29 pKa = 11.84 KK30 pKa = 9.77 ALNACGIAGSVGWIVYY46 pKa = 8.47 WLLKK50 pKa = 10.35 NLSTGRR56 pKa = 11.84 MVANLMGALAIGFCGILMARR76 pKa = 11.84 RR77 pKa = 11.84 KK78 pKa = 9.69 GMPVIIFNIPALVPLVPGATAYY100 pKa = 10.36 RR101 pKa = 11.84 AIRR104 pKa = 11.84 ALVLGNIDD112 pKa = 4.58 LAIQLIVSVTMVAGSIAVGFMLAQLIGEE140 pKa = 4.19 LTVYY144 pKa = 10.64 HH145 pKa = 6.4 SRR147 pKa = 11.84 SLPKK151 pKa = 10.05 LRR153 pKa = 11.84 RR154 pKa = 11.84 PGQRR158 pKa = 11.84 YY159 pKa = 8.44 KK160 pKa = 11.21
Molecular weight: 17.35 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.326
IPC2_protein 9.984
IPC_protein 10.701
Toseland 10.452
ProMoST 10.306
Dawson 10.643
Bjellqvist 10.409
Wikipedia 10.877
Rodwell 10.862
Grimsley 10.73
Solomon 10.716
Lehninger 10.672
Nozaki 10.496
DTASelect 10.394
Thurlkill 10.511
EMBOSS 10.862
Sillero 10.57
Patrickios 10.57
IPC_peptide 10.716
IPC2_peptide 9.663
IPC2.peptide.svr19 8.177
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2031
0
2031
611575
50
2514
301.1
33.58
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
8.723 ± 0.086
0.436 ± 0.013
6.113 ± 0.057
5.086 ± 0.055
4.158 ± 0.052
6.567 ± 0.059
2.465 ± 0.031
6.469 ± 0.058
6.023 ± 0.066
9.827 ± 0.075
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.686 ± 0.023
4.455 ± 0.041
3.851 ± 0.031
5.384 ± 0.077
4.052 ± 0.041
5.574 ± 0.078
6.498 ± 0.078
6.919 ± 0.04
1.094 ± 0.02
3.618 ± 0.039
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here