bacterium F11
Average proteome isoelectric point is 7.44
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 1550 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A202DJG9|A0A202DJG9_9BACT Penicillin-insensitive transglycosylase OS=bacterium F11 OX=1932698 GN=BVX98_06415 PE=4 SV=1
MM1 pKa = 7.44 KK2 pKa = 9.92 KK3 pKa = 10.36 LYY5 pKa = 10.45 VIAFAVIGLLLGSAIADD22 pKa = 3.5 AQEE25 pKa = 4.46 APMSKK30 pKa = 10.71 AEE32 pKa = 4.0 VEE34 pKa = 4.45 AIITQIDD41 pKa = 3.6 SGNYY45 pKa = 8.78 ASGKK49 pKa = 9.53 EE50 pKa = 4.01 RR51 pKa = 11.84 MDD53 pKa = 3.79 LAQEE57 pKa = 4.34 CRR59 pKa = 11.84 DD60 pKa = 3.93 NLPYY64 pKa = 10.73 DD65 pKa = 3.75 YY66 pKa = 10.54 TNDD69 pKa = 3.81 GAIDD73 pKa = 4.57 DD74 pKa = 5.55 DD75 pKa = 5.15 DD76 pKa = 4.27 EE77 pKa = 6.26 AWMDD81 pKa = 3.42 NYY83 pKa = 10.56 CYY85 pKa = 10.67 EE86 pKa = 4.72 NGIPWFRR93 pKa = 11.84 GTAGGSMYY101 pKa = 9.91 WYY103 pKa = 10.23 RR104 pKa = 11.84 DD105 pKa = 2.79 MWQWKK110 pKa = 9.57 GISCPIPVDD119 pKa = 3.54 PVIDD123 pKa = 4.08 GVVNTADD130 pKa = 4.01 LNFIAINWYY139 pKa = 10.19 SEE141 pKa = 3.8 NPNLWADD148 pKa = 3.63 LTGDD152 pKa = 3.74 GDD154 pKa = 4.16 VTSSDD159 pKa = 4.39 LMQLLMNWW167 pKa = 3.28
Molecular weight: 18.73 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.723
IPC2_protein 3.706
IPC_protein 3.719
Toseland 3.49
ProMoST 3.897
Dawson 3.719
Bjellqvist 3.872
Wikipedia 3.681
Rodwell 3.541
Grimsley 3.401
Solomon 3.706
Lehninger 3.668
Nozaki 3.834
DTASelect 4.101
Thurlkill 3.554
EMBOSS 3.694
Sillero 3.834
Patrickios 0.693
IPC_peptide 3.706
IPC2_peptide 3.808
IPC2.peptide.svr19 3.752
Protein with the highest isoelectric point:
>tr|A0A202DNL6|A0A202DNL6_9BACT Uncharacterized protein OS=bacterium F11 OX=1932698 GN=BVX98_02405 PE=4 SV=1
MM1 pKa = 7.5 GPHH4 pKa = 6.62 GSKK7 pKa = 10.39 KK8 pKa = 10.02 KK9 pKa = 10.49 ALCAYY14 pKa = 9.96 LRR16 pKa = 11.84 EE17 pKa = 4.3 QNFPGVLFEE26 pKa = 4.3 FTTFVPSKK34 pKa = 10.32 DD35 pKa = 3.29 LYY37 pKa = 11.22 AGEE40 pKa = 4.27 EE41 pKa = 3.96 CRR43 pKa = 11.84 GIKK46 pKa = 10.31 FKK48 pKa = 10.06 VTNRR52 pKa = 11.84 NTIRR56 pKa = 11.84 PFQIFMVTFLYY67 pKa = 10.74 LPLRR71 pKa = 11.84 SRR73 pKa = 11.84 SQHH76 pKa = 4.47 GQFHH80 pKa = 6.22 GRR82 pKa = 11.84 SAGLGSVPIVCLGHH96 pKa = 7.19 RR97 pKa = 11.84 SHH99 pKa = 6.52 QRR101 pKa = 11.84 TGHH104 pKa = 5.4
Molecular weight: 11.79 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.328
IPC2_protein 9.619
IPC_protein 10.087
Toseland 10.482
ProMoST 10.116
Dawson 10.613
Bjellqvist 10.292
Wikipedia 10.774
Rodwell 10.965
Grimsley 10.657
Solomon 10.672
Lehninger 10.643
Nozaki 10.496
DTASelect 10.277
Thurlkill 10.496
EMBOSS 10.877
Sillero 10.54
Patrickios 10.716
IPC_peptide 10.672
IPC2_peptide 9.399
IPC2.peptide.svr19 8.486
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
1550
0
1550
621162
34
9211
400.7
45.05
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.395 ± 0.064
0.647 ± 0.023
5.489 ± 0.08
6.279 ± 0.081
4.839 ± 0.046
7.209 ± 0.068
2.189 ± 0.031
6.661 ± 0.063
6.423 ± 0.106
10.175 ± 0.107
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.121 ± 0.028
4.283 ± 0.055
4.698 ± 0.043
3.688 ± 0.035
5.391 ± 0.065
6.865 ± 0.071
5.535 ± 0.106
6.588 ± 0.049
1.485 ± 0.024
3.04 ± 0.04
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here