bacterium F11

Taxonomy: cellular organisms; Bacteria; unclassified Bacteria

Average proteome isoelectric point is 7.44

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 1550 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    

Protein with the lowest isoelectric point:
>tr|A0A202DJG9|A0A202DJG9_9BACT Penicillin-insensitive transglycosylase OS=bacterium F11 OX=1932698 GN=BVX98_06415 PE=4 SV=1
MM1 pKa = 7.44KK2 pKa = 9.92KK3 pKa = 10.36LYY5 pKa = 10.45VIAFAVIGLLLGSAIADD22 pKa = 3.5AQEE25 pKa = 4.46APMSKK30 pKa = 10.71AEE32 pKa = 4.0VEE34 pKa = 4.45AIITQIDD41 pKa = 3.6SGNYY45 pKa = 8.78ASGKK49 pKa = 9.53EE50 pKa = 4.01RR51 pKa = 11.84MDD53 pKa = 3.79LAQEE57 pKa = 4.34CRR59 pKa = 11.84DD60 pKa = 3.93NLPYY64 pKa = 10.73DD65 pKa = 3.75YY66 pKa = 10.54TNDD69 pKa = 3.81GAIDD73 pKa = 4.57DD74 pKa = 5.55DD75 pKa = 5.15DD76 pKa = 4.27EE77 pKa = 6.26AWMDD81 pKa = 3.42NYY83 pKa = 10.56CYY85 pKa = 10.67EE86 pKa = 4.72NGIPWFRR93 pKa = 11.84GTAGGSMYY101 pKa = 9.91WYY103 pKa = 10.23RR104 pKa = 11.84DD105 pKa = 2.79MWQWKK110 pKa = 9.57GISCPIPVDD119 pKa = 3.54PVIDD123 pKa = 4.08GVVNTADD130 pKa = 4.01LNFIAINWYY139 pKa = 10.19SEE141 pKa = 3.8NPNLWADD148 pKa = 3.63LTGDD152 pKa = 3.74GDD154 pKa = 4.16VTSSDD159 pKa = 4.39LMQLLMNWW167 pKa = 3.28

Molecular weight:
18.73 kDa
Isoelectric point according different methods:






Protein with the highest isoelectric point:
>tr|A0A202DNL6|A0A202DNL6_9BACT Uncharacterized protein OS=bacterium F11 OX=1932698 GN=BVX98_02405 PE=4 SV=1
MM1 pKa = 7.5GPHH4 pKa = 6.62GSKK7 pKa = 10.39KK8 pKa = 10.02KK9 pKa = 10.49ALCAYY14 pKa = 9.96LRR16 pKa = 11.84EE17 pKa = 4.3QNFPGVLFEE26 pKa = 4.3FTTFVPSKK34 pKa = 10.32DD35 pKa = 3.29LYY37 pKa = 11.22AGEE40 pKa = 4.27EE41 pKa = 3.96CRR43 pKa = 11.84GIKK46 pKa = 10.31FKK48 pKa = 10.06VTNRR52 pKa = 11.84NTIRR56 pKa = 11.84PFQIFMVTFLYY67 pKa = 10.74LPLRR71 pKa = 11.84SRR73 pKa = 11.84SQHH76 pKa = 4.47GQFHH80 pKa = 6.22GRR82 pKa = 11.84SAGLGSVPIVCLGHH96 pKa = 7.19RR97 pKa = 11.84SHH99 pKa = 6.52QRR101 pKa = 11.84TGHH104 pKa = 5.4

Molecular weight:
11.79 kDa
Isoelectric point according different methods:






Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

1550

0

1550

621162

34

9211

400.7

45.05

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.395 ± 0.064

0.647 ± 0.023

5.489 ± 0.08

6.279 ± 0.081

4.839 ± 0.046

7.209 ± 0.068

2.189 ± 0.031

6.661 ± 0.063

6.423 ± 0.106

10.175 ± 0.107

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.121 ± 0.028

4.283 ± 0.055

4.698 ± 0.043

3.688 ± 0.035

5.391 ± 0.065

6.865 ± 0.071

5.535 ± 0.106

6.588 ± 0.049

1.485 ± 0.024

3.04 ± 0.04

Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski